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Citations for Takara Bio :
601 - 650 of 1168 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and ligated into the Nhe1 site of the pBS32-Flag-MS2CP parent plasmid using InFusion cloning (Clontech). The PTBP1 Y247Q ...
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bioRxiv - Bioengineering 2021Quote: ... an endotoxin-free plasmid midiprep DNA purification took place using NucleoBond Xtra Midi EF kit (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The sequence encoding amino acids 2-1273 were cloned into a shuttle plasmid following InFusion cloning (Clontech). The shuttle plasmid encodes a modified human cytomegalovirus major immediate early promoter (IE CMV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was used to transfect cells at 70% confluency with 20 ng of CRE-SEAP reporter plasmid (Clontech) and 20 ng of receptor or empty vector pcDNA-Zeo-tetO DNA per well ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmid assembling steps were performed with the In-Fusion HD Cloning Kit (Clontech, Mountain View, CA, USA). The gene cassettes used for the cell-surface expression of hemicellulases were previously optimized [43 ...
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bioRxiv - Molecular Biology 2021Quote: All PCR amplification and insertion into plasmids were performed with PrimeSTAR MAX and In-Fusion HD (TaKaRa), respectively.
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bioRxiv - Evolutionary Biology 2020Quote: ... the promoter was inserted into pRS41H-lacZ plasmid using an In-Fusion kit (Takara Bio USA Inc.). The pRS41H-lacZ plasmid was constructed by inserting the lacZ fragment amplified from the pSH18-34 plasmid into the pRS41H plasmid (Estojak et al ...
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bioRxiv - Developmental Biology 2020Quote: ... The two DNA fragments were then ligated into one plasmid with the InFusion HD cloning kit (Takara).
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bioRxiv - Neuroscience 2020Quote: ... The APP-mCherry plasmid was generated by introducing the APP751 sequence in the pmCherry-N1 vector (Clontech) at the XmaI/AgeI restriction site ...
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bioRxiv - Cell Biology 2019Quote: ... CDCP1 and its respective mutants were subcloned into either the pEGFP-N1 or pmCherry-N1 plasmid (Clontech) and then subcloned into the pRetroX-TRE3G retroviral plasmid (Clontech) ...
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bioRxiv - Neuroscience 2022Quote: ... were amplified by PCR and inserted into a CAG-promoter plasmid backbone by In-Fusion HD (Takara). A plasmid encoding hWDR62-FLAG was generated by amplifying hWDR62-FLAG by PCR ...
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bioRxiv - Plant Biology 2022Quote: ... The downstream genomic region was then amplified using the primer set MpHEC1_CDS_IF_F and MpHEC1_3UTR_IF_R2 and integrated into the first binary plasmid with an In-Fusion HD Cloning Kit (Clontech) using the BamHI and AscI sites ...
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bioRxiv - Biochemistry 2022Quote: ... pDONR/Zeo (Supplementary Table 1) plasmid with the desired mutation was done via In-Fusion technology (Takara). Each pDONR/Zeo Cac1 mutant plasmid was verified by Sanger sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... pDESTTM32RAPTOR1BcDNAwas co-transformed either with pDESTTM22FLZ14cDNA or pDESTTM22S6K1cDNA plasmids into the yeast strain Y2HGold (Takara Bio, USA) as previously described (Paiano et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... FD0564)-digested pCRIS-PITChv2-FBL plasmid (addgene #63672) using the In-Fusion HD cloning kit (Takara Bio, 639650). To create the sgRNA-plasmid backbone ...
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bioRxiv - Bioengineering 2022Quote: Arc DNA plasmids were transfected into the donor cell culture via PEI or Xfect transfection reagent (Takara) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The plasmids of EcOpgD and EcOpgG mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Immunology 2023Quote: ... The fragments were gel purified and then assembled into a plasmid using in-fusion reagents (Takara Bio). The mCherry11-RNase L and mCherry1-10-RNase L were made similarly with oligos in Supplementary table 1 ...
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bioRxiv - Genetics 2023Quote: ... Plasmids that contain each sgRNA were transfected to HEK293T cells using Lenti-X packaging single shots (Takara) for viral packaging ...
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bioRxiv - Microbiology 2023Quote: The plasmid constructs used as repair template for recombinant virus generation were generated using InFusion cloning (TaKaRa). The sequence references below are based on the HSV-1 KOS genome accession number JQ673480 (Macdonald et al 2012) ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were generated using Gibson assembly and In-Fusion cloning according to manufacturer protocols (Takara, 638920). PB-NCT-GFP plasmids were generated using NCT plasmids donated by Zane Zeier and were flanked by PiggyBac transposon arms ...
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Molecular Biology 2024Quote: ... We then introduced into this 303_p3E_GI_PA our previous RNAi cassette from plasmid pME-RNAi6415 using an “In-fusion (Takara)” cloning strategy ...
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bioRxiv - Molecular Biology 2021Quote: ... transferred onto nitrocellulose and detected with either mouse anti-6×His (Clontech) at 0.25 µg/ml or rabbit antibody against calmodulin binding peptide Calmodulin Binding Peptide (GenScript ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with mouse anti-GFP (1:500, Takara Bio, 632380), rabbit anti-mCherry (1:500 ...
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bioRxiv - Immunology 2022Quote: ... The SMARTer Mouse TCR a/b Profiling Kit (Takara, Cat. No. 634403) was used to amplify both TCRα and TCRβ sequences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Sections were incubated overnight with mouse anti-STEM121 primary antibody (Takara, Y40410) and then developed with a DAB substrate kit (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... The mouse PG cDNA was generated by PrimeSTAR Max DNA Polymerase (Takara) to skip 150 bp ...
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bioRxiv - Cell Biology 2020Quote: Plasmids were constructed using standard molecular biology methods including polymerase chain reaction (PCR) and In-fusion cloning (Clontech). Mutations for specific residues were introduced by overlap-extension PCR ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids encoding the bait (pDEST32) and prey (pDEST22) were transformed into the yeast strain AH109 and Y187 (Clontech) by the LiAc method following the protocol in the MATCHMAKER GAL4 Two Hybrid System 3 manual (Clontech) ...
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bioRxiv - Genetics 2019Quote: ... the BirA-R118G open reading frame from the BirA(R118G)-HA destination vector (gift from Karl Kramer (plasmid # 53581, http://www.addgene.org) was subcloned into the retroviral vector pQCXIN (Clontech) to generate either N-terminal (pQCXIN-BirA-Myc-N ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid called “CFP-Golgi” (containing amino acids 1–81 of ß-1,4glycosyltransferase) was originally purchased from Clontech.
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bioRxiv - Systems Biology 2021Quote: ... Other plasmid components (see Supplementary Table 1) were synthesized or PCR amplified with PrimerSTAR MAX DNA polymerase (TAKARA) and checked by Sanger sequencing ...
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bioRxiv - Biochemistry 2020Quote: The full-length L1 fragments of 15 high-risk HPV (hrHPV) were cloned in the pMD plasmid (Takara) to prepare HPV plasmids ...
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bioRxiv - Biochemistry 2020Quote: ... the cDNA encoding Halo7-β2AR-FKBP_F36V (β2AR-FKBP) was cloned into the expression plasmid vector pEGFP-N1 (Clontech) after the removal of the cDNA region encoding EGFP ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid pIRESpuro3-EGFP was constructed by excising the EGFP open reading frame from pEGFP-C1 vector (Clontech) with NheI and BamHI digestion and insertion into the same restriction sites of the pIRESpuro3 vector (Clontech ...
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bioRxiv - Neuroscience 2020Quote: ... a series of FRT-FP plasmids were constructed (Fig. S1) using the mammalian expression backbone pCMV-N1 (Clontech). FRT-F13 ...
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bioRxiv - Neuroscience 2020Quote: ... #AM9937).The Brp-T2A-QF2w-Hsp70-3XP3-dsRed donor plasmid was constructed using the InFusion HD Kit (Clontech, #638910). Homology arms of ∼1 kb flanking the sgRNA cut site were cloned by PCR using template genomic DNA from the Orlando strain ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA to generate viral vectors was prepared using the NucleoBond Xtra Maxi endotoxin free kit (Takara Bio) prior to production ...
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bioRxiv - Microbiology 2021Quote: ... The cassette was PCR-amplified from these plasmids utilizing TaKaRa Ex Taq™ DNA Polymerase (Clontech Takara Bio) and used for A ...
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bioRxiv - Microbiology 2021Quote: ... The cassette was PCR-amplified from these plasmids utilizing TaKaRa Ex Taq™ DNA Polymerase (Clontech Takara Bio) and used for A ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg each CAS9 guide RNA plasmid (pAS4883 and 4884) and 200 ng linear hygromycin resistance gene (Clontech) were used ...
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bioRxiv - Plant Biology 2021Quote: ... Bait and prey plasmids were co-transformed into the yeast strain AH109 according to the manufacture’s introduction (Clontech). Transformants were selected on SD (-Leu/-Trp ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were transfected with pX330 vector together with an EGFP-containing plasmid (pEGFP-N1; Clontech Laboratories, Inc). Single cells were sorted using a FACS Vantage SE machine and the knockouts confirmed by sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... cells were plated in 24-well plates and transfected with 1.5 μg/μl of psDsRed-2Mito plasmid (Clontech).
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bioRxiv - Microbiology 2022Quote: All plasmid inserts were amplified by PCR using standard PCR conditions and the CloneAmp HiFi PCR premix (Takara). Following a PCR purification step (QIAquick PCR purification kit) ...
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bioRxiv - Microbiology 2022Quote: ... CoV-229E sequences were obtained as gBlocks from IDT and subcloned into these plasmids using InFusion cloning (Takara).
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bioRxiv - Plant Biology 2021Quote: Plasmids for Citrine or mNeon-Green endogenous tagging were assembled using In-Fusion (Clontech, Mountain View, CA, USA), in which Citrine or mNeonGreen genes ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR-amplified DNA fragments were assembled and cloned into pET-in situ Ble plasmid by recombination (InFusion, Clontech) and the resulting cell line was named ABbin situ ...