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Citations for Takara Bio :
451 - 500 of 1523 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... human CCDC22 was first cloned into pEGFP-C1 (Clontech) between Sal1 and BamH1 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific GFAP (hGFAP, TAKARA, Stem123, 1:1000), anti-AQP4 (Cell Signaling ...
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bioRxiv - Genomics 2024Quote: ... Human Fetal Brain Total RNA (Takara Bio USA, #636526) and Human Brain Total RNA (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: Primers (PAGE grade, fasmac, Appendix Table S4) were labelled with 32P-γ-ATP by T4PNK (Takara), followed by filtering through MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was amplified with a SYBR Green dye kit and gene-specific primers (Takara, Shiga, Japan). Data were normalized to β-actin mRNA levels ...
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bioRxiv - Microbiology 2021Quote: ... specific primers were designed and PCR reactions were performed using SYBR Green Premix Ex Taq (Takara), according to the manufacturer’s protocol and the primers listed in Table S4 ...
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bioRxiv - Plant Biology 2021Quote: ... The gRNA - dCas9 complex was amplified (primers in Supplemental Table 1) and In-Fusion cloned (Clontech) into a SacI digested pMJS064 (described above ...
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bioRxiv - Microbiology 2021Quote: ... plasmids respectively using primers HindIII_ZIKV_sfRNA and XbaI_GFP (Supplementary table 7) and PrimeSTAR GXL Polymerase (Takara, Japan). Cycling conditions were as follows ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixture contained 10 μL of SYBR® Primer EX Taq II (Takara, Japan, RR420A), 0.4 μL ROX Reference Dye (50× ...
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bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was reverse transcribed using oligo(dT) primers with the SMARTer Ultra Low Input system (Takara). The cDNA was then sheared with a Covaris S2 ultrasonicator ...
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bioRxiv - Molecular Biology 2021Quote: Primers were designed using the Takara Bio Perfect Real Time Support System (Takara Bio, Table 2). Primers were diluted to 50 µM in ddH20 and stored at -20°C ...
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bioRxiv - Evolutionary Biology 2021Quote: Amplified with V4 16S rRNA primers as above using EmeraldAmp GT PCR Master Mix (TAKARA BIO).
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bioRxiv - Immunology 2024Quote: ... Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A, Takara). β-actin was used as the internal quantitative control.
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bioRxiv - Plant Biology 2023Quote: ... Fragments amplified with primers for CAPS markers were digested with appropriate restriction enzymes supplied by Takara Bio (Kusatsu ...
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bioRxiv - Developmental Biology 2024Quote: ... and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... 10 μM Reverse primer and TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara, #RR820W). Real-Time PCR was set up in a 384-well plate and run in a CFX384 Real-Time PCR System (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... under standard conditions with oligo(dT) or random hexamer primers and Recombinant RNase Inhibitor (RRI, TAKARA). Then the cDNA was subjected to quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Biochemistry 2023Quote: ... MeSUFC and MeSUFDSU with specific primers for each gene using PrimeSTAR Max DNA Polymerase Premix (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... cDNA and specific primers were mixed with a TB Green Premix Ex Taq (Takara, Otsu, Japan). PCR reactions were run on a Smart Cycler System (Takara) ...
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... cDNA was amplified with V3d(T)24 and R2SP primers and Terra PCR Direct Polymerase (Clontech) for 16 cycles ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA and primers were mixed with TB Green® Premix Ex TaqTM II (Takara, China), and RT-qPCR was conducted on the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... JEG3 cells (human; sex: female, placenta epithelial), HEK293 derivative Lenti-X™ 293T cells (human; sex: female, kidney epithelial) obtained from Takara (cat. 632180), Huh-7.5 heptoma cells (human ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... VSV G-pseudotyped HIV-1 using full-length HIV-1 molecular clone pNL4-3 [79] or the R5-tropic pNL4-3 AD8 derivative [80] and VSV-G envelope encoding plasmid (PT3343-5, Clontech); 4 ...
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bioRxiv - Biophysics 2022Quote: ... 5’ and 3’-RACE-Ready cDNA templates were synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 5’ and 3’ end sequences of P ...
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bioRxiv - Cell Biology 2020Quote: ... with 5’-cgGAATTCaccATGgagcagaagctc atctcagaagaagacctcggtAAGGATAACACCGTGCC-3’ and 5’-ataagaatGCGGCCGCtaaact ATTATTTTTCTGCACTACGCAGG-3’ followed by cloning into the EcoRI and Not I sites of pIRESpuro3 (Clontech) creating pIRESpuro3-myc-BirA ...
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bioRxiv - Molecular Biology 2020Quote: ... containing the 5’ end was used and the 3’ of TciALDO cDNA was obtained by 3’ RACE (SMARTer RACE cDNA Amplification Kit, Clontech) using T ...
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bioRxiv - Neuroscience 2021Quote: ... the promoter pgrd-10 was amplified from fosmid WRM0612bC07 using 5’-ccatgattacgccaatcgtcatc-3’ and 5’-tggccaatcccggggtttttaga-3’ and cloned with Gateway backbone amplified using 5’-ccccgggattggcca-3’ and 5’-ttggcgtaatcatgg-3’ to make pgrd-10∷GW [pNBRGWY151] using infusion cloning(Takara). It was then recombined with ced-10 WT[pNBRGWY88] using LR recombination (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... and those targeting the Alu repeat sequence in the nuclear genome (5′-CTTGCAGTGAGCCGAGATT-3′ and 5′- GAGACGGAGTCTCGCTCTGTC-3′) (75) with TB Green Premix Ex Taq II (TaKaRa) on Thermal Cycler Dice Real Time System II (TaKaRa) ...
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bioRxiv - Molecular Biology 2023Quote: ... the 3’ UTR of the virus were determined using the SMARTer®RACE 5’/3’ Kit (Takara Bio, Kusatsu, Japan). 2.4 µg of RGMoV gRNA was polyadenylated using Poly(A)polymerase (600 u/µl ...
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bioRxiv - Immunology 2022Quote: ... or the expression vector LentiCRISPRv2 (coding for guideRNA) in a 3:1:3 ratio for 8 hours using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
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bioRxiv - Cell Biology 2023Quote: ... was PCR-amplified with the primer set (fwd: 5’- CTTCGAATTCTGGCCACCATGGCTGCCGCCACCACC-3’, rev: 5’- CGGTGGATCCccCAAGAAATCCTTGATGTTAAGATCCGCTAATGG-3’) and inserted into EcoRI and BamHI sites of pmCherry-N1 (Clontech) by restriction digestion and T4 ligation ...
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bioRxiv - Neuroscience 2024Quote: ... Full-length of itga2 was inserted into the Sfi IA (5′-GGCCATTACGGCC-3′) and Sfi IB (3′-GGCCGCCTCGGCC-5′) sites of the “prey” pPR3-C vector (Clontech). Series of combinations of bait and prey constructs were cotransformed into the yeast strain NMY51 (Clontech) ...
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bioRxiv - Plant Biology 2020Quote: ... and used for qPCR using TB green Premix Ex Taq II (Takara Bio, CA, USA) on Bio-Rad CFX 96 C1000 ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reactions were performed using the SYBR Premix Dimer Eraser kit (Takara, Beijing, China). The relative quantities of the PCR products were calculated using the 2-ΔΔCt method ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa) on the StepOnePlus Real-time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... RT–qPCR analysis was performed using a Thermal Cycler Dice Real Time System TP800 (Takara). The expression levels of genes of interest were normalized relative to those of UBC21 ...
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bioRxiv - Biochemistry 2020Quote: ... and qPCR was performed using TB Green Premix Ex Taq (Tli RNaseH Plus) (Takara, RR420) with the CFX96 Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using SYBR® Premix Ex Taq™ (TaKaRa) in a Bio-Rad CFX96™ real-time PCR system using the following thermocycling conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-qPCR was performed using TB Green® Premix Ex Taq™ Kit (TaKaRa RR420A) on a Applied Biosystems 7500 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2021Quote: High-throughput quantitative PCR (qPCR) was performed using the SmartChip system (Takara Bio, Kusatsu, JAPAN) on 47 oral melanoma samples to detect focal amplifications on tumor DNA on CFA 10 and CFA 30 ...
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bioRxiv - Pathology 2022Quote: ... PCR reactions were conducted with SYBR qPCR Premix Ex Taq II Tli RNaseH+ (TAKRR820W,Takara). Primer pairs are listed in Table S2 ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa) and primers are shown in table S2.
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bioRxiv - Microbiology 2022Quote: ... was performed using one-step Prime script III RT-qPCR mix (RR600A, Takara, Kyoto, Japan). The viral RNA of nucleocapsid protein was detected by a 2019-nCoV-N1 probe (10006770 ...
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bioRxiv - Molecular Biology 2021Quote: ... The viral RNA quantification was performed using One Step PrimeScript III RT-qPCR Kit (Takara). All reactions were performed on a CFX96 Touch instrument with the following quantitative-PCR conditions ...
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bioRxiv - Immunology 2022Quote: ... Viral Titers were determined with the BacPak qpcr Titration kit (Takara Bio, Kusatsu, Shiga, Japan). Infected Sf21 cells were collected and lysed in dPBS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time qPCR was performed in optical 96-well plates using a TP950 (Takara, Japan). Fluorescence threshold data (Ct ...
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bioRxiv - Microbiology 2020Quote: The HSV-1 copy number was examined by qPCR using SYBR/ROX (RR82LR; Takara, Japan) on ABI qPCR machine (Lifetech ...
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bioRxiv - Physiology 2021Quote: ... Purified AAVs were titered using a qPCR based kit (Cat. No. 6233; Takara Bio, USA). The TA muscle of each mouse received a 5 x 1010 vg of either AAV-GFP or AAV-CAAHR using several small volume (~7-8 μl ...
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bioRxiv - Biochemistry 2021Quote: ... real-time qPCR was performed using SYBR premix ex-Taq-ii (Takara Bio Inc., Japan) and a PCR detection system (Bio-Rad ...