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Citations for Takara Bio :
451 - 500 of 535 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... cDNA was generated from 10 ng of RNA using the SMART-Seq v4 UltraLow Input RNA Prep kit (Takara Bio Inc) per manufacturer’s suggestions ...
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bioRxiv - Physiology 2021Quote: ... 10 ng of obtained RNA was processed using the SMARTer Stranded RNA-seq kit for Illumina (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: RNA-seq libraries were prepared from 10 ng total RNA using the SMARTer Stranded Total RNA-seq Pico Input Mammalian V2 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... ceranae-inoculated workers’ midguts at 7 dpi and 10 dpi were respectively isolated using RNA Extraction Kit (TaKaRa company, Dalian, China). cDNA was synthesized through reverse transcription with oligo dT primer and used as templates for qPCR assay ...
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bioRxiv - Neuroscience 2021Quote: ... Quantification of P11+1 SH-10 cells showed that 76% of cells were expressing hLAG3 upon induction by 1 µg/ml of Doxycycline (DOX; Clontech #631311). This decreased to 59% in P11+2 and remained around 50% in the following passages ...
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bioRxiv - Immunology 2022Quote: ... for continuous fluorescence detection in a total volume of 10 μL of cDNA/control and gene-specific primers using SYBR Premix Ex Taq (TaKaRa Bio). The mRNA levels of the target genes were normalized relative to those of Gapdh using the following formula ...
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bioRxiv - Genomics 2021Quote: ... After 48 h the medium was collected and viral supernatant was concentrated 10-fold using the lenti-XTM Concentrator (Takara Bio) following the manufacturer’s instructions and subsequently stored at −80 °C.
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bioRxiv - Plant Biology 2021Quote: ... were mixed in a binding buffer (20 μL) containing 5 μL of 10×CutSmart® buffer and 1μL of RNase inhibitor (40U, Takara, Japan) and left for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using primers C.9 and C.10 and cloned into pOPINF using the In-Fusion HD EcoDry cloning kit (Takara Bio).
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bioRxiv - Cell Biology 2020Quote: ... was added to the StrePD beads and incubated at 37 °C for 10 minutes followed by an incubation with Proteinase K (Takara, #9034) at 60 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... foetal bovine serum or 10% (v/v) TET System–approved FCS for U–2–OS reporter cell lines (631106, Takara Bio). U–2–OS-pEP15 cells (5 ...
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bioRxiv - Cell Biology 2021Quote: Previously described MEFs-IRE1-mNG and U2OS-IRE1-mNG cell lines (14, 27) were cultured in high glucose DMEM media supplemented with 10% tetracycline-free fetal bovine serum (FBS; Takara Bio), 6 mM L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted and reverse transcription and cDNA amplification were performed using 10 ng of RNA using the SMARTer Ultra Low RNA kit (Clontech Laboratories) like previously reported 13 ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 10 ng DNA from the ε2/ε2 and ε3/ε3 cell lines with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... The beads were then resuspended in a total volume of 10 µL for library preparation using the SMARTer ThruPLEX DNA-seq library preparation kit (Takara Biosciences) per manufacturer’s protocol with an adjustment on the last step ...
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bioRxiv - Cell Biology 2022Quote: For transient rescue of the ATG9A KO cell line, 3xFLAG-ATG9A was subcloned from pCMV-10-3xFLAG-ATG9A (described in (Ghanbarpour et al., 2021)) into pLVX-puro (Clontech; 632159) for stable cell line creation ...
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bioRxiv - Genomics 2022Quote: ... The ligation reactions were heat-inactivated at 65°C for 10 minutes and cleaned up with NucleoMag NGS Clean-up and Size Select beads (Takara Bio) before electroporation into Endura Electrocompetent E ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was reverse transcribed into 10 μL of complementary DNA (cDNA) with PrimeScriptTM RT Master Mix (Takara Biomedical Technology, China) at 37°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Libraries for mRNA sequencing were prepared starting from 10 ng tot RNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). All samples were sequenced on an Illumina NextSeq 550 at an average of 17,5 million 75-bp single-end reads.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in a 20 µL reaction consisting of 10 μL of TB Green Premix Ex Taq 2 (TliRNaseH Plus; TaKaRa, Dalian, China), 1 µL each of forward and reverse primer ...
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bioRxiv - Synthetic Biology 2024Quote: ... The mixture (1 μL) was used as the template in 10 μL of PrimeSTAR GXL DNA Polymerase (Takara Bio Inc., Japan) reaction ...
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bioRxiv - Cell Biology 2024Quote: ... U-2OS cells stably infected with FLAG-CCND1/2xStrep-CCND2/HA-CCND3 were maintained in McCoy’s 5A medium supplemented with 10% Tet System Approved FBS (Takara, Clontech Laboratories) and 1% penicillin/streptomycin/L-glutamine (Corning Life Sciences) ...
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs from HeLa cells (about 5 x 107 cells/10 cm dish) transfected with scrambled siRNA (si-Ctrl) or si-CDC5L were extracted using RNAiso Plus (Takara, 9109) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Only 0.5 µL of media containing cumulus cells from each mouse was added to 10 µL lysis buffer (Takara Bio Inc.). The oocytes were then treated with 1 mg/mL collagenase to remove the ZP ...
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries are prepared by incorporating 1-10 ng of cfDNA with the ThruPLEX® Plasma-seq Kit by Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... 30 cycles of 98 °C for 10 sec followed by 68 °C for 1 min using PrimeSTAR HS DNA Polymerase with GC buffer (Takara, R044A). The primers used for the purpose of inserting Capn4 into pCWX200 and pLexA were ...
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bioRxiv - Cell Biology 2023Quote: ... 30 cycles of 98 °C for 10 sec followed by 68 °C for 1 min using PrimeSTAR HS DNA Polymerase with GC buffer (Takara, R044A). The primers used were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... The lentivirus was produced in DMEM containing 10% FBS and 1% BSA and then concentrated by the Lenti-X concentrator (Takara Bio).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The reaction volume of 20 µL consisted of 10 µL of TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara, Japan), 0.8 µL each of gene-specific primers (10 µM) ...
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bioRxiv - Genomics 2023Quote: ... Lentiviral supernatant was centrifuged at 600xg for 10 min to remove cellular debris and concentrated to 50-100x the initial concentration using Lenti-X Concentrator (Takara Bio). T cells were transduced at 5-10% v/v of concentrated lentivirus at 24 hours post-activation ...
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bioRxiv - Genomics 2023Quote: ... of the resulting ligations as well as the control were transformed into 10 ul of Stellar chemically competent cells (Takara #636763) using manufacturer guidelines ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was spun at 5000 rpm for 10 min to remove cell debris and concentrated via Lenti-X as per the instructions (Takara Bio). HIV-Gag-iGFP_JRFL (the following reagent was obtained through the NIH HIV Reagent Program ...
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bioRxiv - Biochemistry 2024Quote: ... for A3>P: 5’-aaaacacugaaccug-3’ for A2>P: 5’-aaacacugaaccug-3’) were incubated with 10 U recombinant MazF (Takara Bio) in 20 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2024Quote: ... we used a 2:1 molar ratio of insert to SalI/AgeI-digested pTYC10 (125 ng of pPromALB_STARRseq plasmid; Table S1E) in a single 10 μl In-Fusion HD reaction (Clontech, cat. # 639650) via the homology arms ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentration was assessed by Nanodrop and RNA-seq libraries were prepared from 10 ng of total RNA using the SMARTer Stranded Total RNA-seq Pico Input Mammalian V2 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... PCR experiments were performed in a total volume of 10 μl by using an SYBR Premix Ex Taq kit (Takara Bio). The reaction mixtures were incubated for 2 min at 95℃ ...
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bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...
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bioRxiv - Neuroscience 2021Quote: ... and 45 cycles from 10 s at 95°C to 30 s at 60°C using a TB Green Premix Ex TaqTM GC (Takara Bio, Japan).
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bioRxiv - Neuroscience 2021Quote: ... and 45 cycles from 10 s at 95°C to 30 s at 60°C using TB Green Premix Ex TaqTM GC (Takara Bio, Japan).
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bioRxiv - Genomics 2021Quote: ... the synthesized cDNA products were subjected to real-time PCR in a reaction mixture (10 μl) containing Takara Premix Ex Taq (Probe qPCR) (TaKaRa, Kyoto, Japan), 200 nM hydrolysis probes and 200 nM PCR primers ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells grown in 10 ml of medium (3.0 x 105 cells/ml) were transfected with 10 µg of pCAGGS-NP (1-450) using TransIT-293 Reagent (Takara, Shiga, Japan). Three days post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... vector used was AAV9-CaMKII::G-CaMP7 (9.4 × 1012 vg/mL)35 after 10-fold dilution with phosphate buffered saline (PBS) (T900, Takara BIO INC, Japan) before injection ...
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A New Gene Set Identifies Senescent Cells and Predicts Senescence-Associated Pathways Across TissuesbioRxiv - Cell Biology 2021Quote: ... 86% of 2% Tween 20 in deionized Water) or AP20187 (B/B homodimerizer, Clontech; 10 mg of AP20187 per kg body mass) twice weekly at the age of 20 months for a total of 4 months (old mice were sacrificed at 24 months of age) ...
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bioRxiv - Biochemistry 2021Quote: ... RNC 82-aa C34A with [SG]5 or [SG]10 repeats were constructed by the Prime STAR MAX (Takara Bio Inc., Japan) method using appropriate primers (Table 1).
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bioRxiv - Synthetic Biology 2020Quote: ... The deletion of the lacZ gene was verified using blue-white selection on an LB agar plate containing 0.1 mM isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque) and 4.0 × 10−3 % 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Takara Bio).
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bioRxiv - Evolutionary Biology 2021Quote: ... The decanted soluble extract was adjusted to a volume of 90 mL with lysis buffer and incubated with 10 mL TALON cobalt resin (Takara, Shiga, Japan) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... S domains and variants were purified similar to S protein using nickel resin (10 mL/L, His60 Ni2+ superflow resin, Takara cat# 635664) with wash buffer (25 mM MES ...
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bioRxiv - Molecular Biology 2022Quote: ... and amplified by PCR using a forward primer containing a T7 promoter sequence (Supplemental Table 10) with the Advantage HF 2 kit (Takara Bio 639124) and the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated DNA was amplified by PCR using Hot Start TaKaRa LA Taq kit to yield a 10-Kb product (Takara Biotechnology, #RR042A). Primers utilized were the following ...