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Citations for Takara Bio :
1 - 50 of 535 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The membrane was then pre-hybridized in 10 mL ExpressHyb (Clontech, NC9747391) at 44°C in a rotating oven for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then pre- hybridized in 10 mL ExpressHyb (Clontech, NC9747391) at 44°C in a rotating oven for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: Media was collected 48 h after transfection and clarified by syringe filtration through a 0.45 µm polyvinylidene difluoride membrane (Millex-HV) before concentrating the virus with a Lenti-X concentrator (Clontech 631231). The concentrated virus was resuspended in PBS and aliquoted into single-use tubes stored at −80 °C ...
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bioRxiv - Neuroscience 2020Quote: ... We coated 6-8 mg of 1.6 µm gold beads with 10-15 µg of EGFP-N1 (Clontech) or 20 μg pSuper-cofilin1-shRNA + 20 μg pSuper-ADF-shRNA (Bosch et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were transfected on DIV11-13 using CalPhos Mammalian Transfection kit (Takara) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... the pellet was re-suspended in four millilitres of filtered-sterilized (0.22 µm filter) lysing buffer (75 mg/mL BSA; 10 mg/mL Yatalase (Takara); 5 mg/mL Lysing Enzymes (L1412 Sigma) ...
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bioRxiv - Pathology 2021Quote: ... 5 µL of 1:10 diluted cDNA samples was used as the qRT-PCR template with 0.5 µM gene-specific primers and 10 µL SYBR Premix Ex Taq II (Takara, China) in a total volume of 20 µL ...
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bioRxiv - Cell Biology 2024Quote: ... Hippocampal neurons were transfected on DIV11-13 using the CalPhos Mammalian Transfection kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Shield1: 1.5-3 µM (Takara). With the exception of time-course experiments ...
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bioRxiv - Cancer Biology 2022Quote: SV40-MES-13 cells were lysed with xTractor Buffer Kit (CLONTECH Laboratories, Inc., CA, USA). Protein specimens were quantified through BCA method and electrophoresed through 10% SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µM Shield-1 (Takara # 632189) for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... 2 µL 100 µM DTT (Takara), 2 µL 10 µM template switching oligo ...
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bioRxiv - Molecular Biology 2023Quote: ... and fragments were cloned into the pSAM2-Puro lentiviral vector 13 by In-Fusion HD Cloning (Takara).
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bioRxiv - Systems Biology 2023Quote: ... heterodimerized with 0.5 µM AP21967 (Takara, 635057) on Day 6 ...
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bioRxiv - Microbiology 2024Quote: ... the LHA and RHA PCR products were used as primers for amplifying the tsp exchange gene using CloneAmp HiFi PCR Premix (Takara Bio). After five PCR cycles ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: An RNA blot membrane (Clontech) was hybridized with the 32P-labeled full coding sequence of rMap7D2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The 13 kb band was purified with gel purification kit (Takara Nucleospin® Gel and PCR Clean-up Midi). Eighty-two base pair single stranded oligos were designed according to the following structure:
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bioRxiv - Cancer Biology 2024Quote: ... The 13 kb band was purified with gel purification kit (Takara Nucleospin® Gel and PCR Clean-up Midi). The insert encoding the gRNA sequences was prepared by annealing TOP and BOTTOM oligonucleotides followed by T4 PNK phosphorylation (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... 9, 11, 13, 20, or 45 (SERF2, C9orf16, C19orf53, C11orf58, BEX3, or SERBP1 respectively) was inserted into pCold I (Takara), together with the client protein ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was amplified by PCR in separate 25 μL reactions each containing 2 μL of cDNA for 11-13 cycles using 0.5 μL (2.5 U) LA Taq (Takara, Cat# RR002M) with P5-3’ and miRCat-PCR-2 oligos at an annealing temperature of 58°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced with 1 µM rapalog (AP21967, Takara) for 45 minutes at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... cultures were spiked with 0.5 µM AP21967 (Takara, 635057) or 0.1% ethanol vehicle for 24 hours before cell lysis and biochemical analysis.
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bioRxiv - Systems Biology 2023Quote: ... cultures were spiked with 0.5 µM AP21967 (Takara, 635057) or 0.1% ethanol vehicle ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm in thickness) were processed for immunodetection of Mouse anti-GFP antibody (1:500, 632381, Takara Bio USA, Inc. Mountain View, CA) and incubated with secondary Donkey anti-Mouse Alexa Fluor 488 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from tissues of 13-month-old wildtype C57BL/6J mice (n=3) using RNAiso Plus (Takara Bio). Complementary DNA (cDNA ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were hybridized in ExpressHyb Hybridization Buffer (ClonTech) to random-primed 32P-labeled DNA probes (RadPrime DNA labelling system ...
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bioRxiv - Biophysics 2020Quote: ... Solubilized membranes were incubated with Talon resin (Clontech) and then with 100 μM TCEP reducing agent to obtain A2AR-bound Talon resin suspended in 50 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were hybridized in ExpressHyb Hybridization Buffer (ClonTech) to random-primed 32P-labeled DNA probes (RadPrime DNA labelling system ...
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bioRxiv - Immunology 2024Quote: ... The membrane was developed with ECL reagent (Takara). Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 µM of Shield-1 peptide (Clontech, Mountain View CA) for 4-6 hours ...
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bioRxiv - Immunology 2021Quote: ... The membranes were subsequently stripped (Takara BioInc, Kusatsu, Japan), blocked ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated with 32P-radiolabeled ura4 probe (TaKaRa BcaBESTTM Labeling Kit ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were incubated with mouse anti-GFP (Clontech #632380), rabbit anti-DsRed (Takara #632496) ...
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bioRxiv - Bioengineering 2024Quote: ... The human KRT14 enhancer and promoter sequence [13] [14] was amplified using human genomic DNA from HEK293 cells with PrimeSTAR GXL DNA polymerase (Takara Bio Inc., Shiga, Japan). The human KRT16 pseudogene 5 (K16P5 ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of dNTP mix (TaKaRa, 2.5 mM concentration, 200 µM final), 0.125 µl of HotStart ExTaq (TaKaRa ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed thoroughly and coated in 50□µM iMatrix-511 (Clontech) until neuron plating.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then treated with 1 µM Shield1 (Takara Bio Cat#632189) 5 days post-BFP sort then harvested for flow cytometry and Western blot analysis 3 days post-Shield1 treatment ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol) containing DNase I (10 U, Takara) and incubated at 37 °C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then blocked in ExpressHyb hybridization solution (Clontech) for 1 hour.
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bioRxiv - Microbiology 2024Quote: ... The membrane was probed with either anti-Flag (Clontech, Japan) or anti-Myc (Santa Cruz Biotechnology ...
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bioRxiv - Systems Biology 2023Quote: ... 1.5 coverslips and the next day treated with 0.5 µM AP (Takara, 635057) for the indicated times where indicated ...
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bioRxiv - Neuroscience 2024Quote: ... per reaction and 2 µl of 5 µM SMART PCR primer (Takara Bio). Amplification was performed by incubating tubes on a thermocycler at 98°C for 3 minutes followed by 18 cycles of 98°C for 20 seconds ...
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bioRxiv - Biophysics 2021Quote: ... 10% FBS (Clontech), 50 U/mL penicillin ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated in anti-GFP (Takara Bio, 1:5000 dilution) antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with anti-GFP antibody (JL-8; Clontech) at a dilution of 1:5,000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membrane was incubated with an α-GFP primary antibody (Takara Bio cat# 632380 ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were stripped using Western BLoT Stripping Buffer (Takara Bio, #T7135A) and re-probed with the desired antibody ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was incubated with Western BLoT Chemiluminescence HRP Substrate (TaKaRa Bio), and membrane image was captured using a chemiluminescent imaging system LuminoGraph I (ATTO ...
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bioRxiv - Microbiology 2024Quote: ... the solubilized membrane fraction gets incubated with TALON SuperFlow resin (Takara 635502) for 1 hours at 4°C and was later washed using buffer B supplemented with 20 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane was then incubated with 1:1000 mouse anti-His6 antibody (Clontech) for 15 mins ...