Labshake search
Citations for Lonza :
201 - 250 of 377 citations for Mouse MYCN shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were electroporated with 20 ug of guide plasmid and 60 ug of linearized repair plasmid using an Amaxa 4D electroporator and P3 Primary cell 4D Nucleofector X Kit L (Lonza) using programme FP158 as described (Collins et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended and transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution in a 25 µL electroporation cuvette (Lonza). Electroporation of GCaMP mutants was performed according to the manufacturer’s protocol.
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bioRxiv - Genomics 2019Quote: ... one million cells were mixed with 20 μg plasmids and 100 μL supplemented Nucleofector Solution V and electroporated with a Nucleofector II device (Lonza) using Program T-016 ...
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bioRxiv - Microbiology 2020Quote: ... 3 x 107 bloodstream form cells were harvested by centrifugation and transfected with 5-10 μg of linearized plasmid DNA using an Amaxa Nucleofector II (Lonza) with program X-001 ...
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bioRxiv - Immunology 2021Quote: Control or transfected Lymphocytes (with Flag-wt-TDP-43 or Flag-NLS-mut-TDP-43 plasmid (1 μg) using nucleofection kit (Lonza)) were placed on coverslips (2 x 106 cells in sterile glass coverslips-Ø 12 mm ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution electroporation cuvettes (Lonza). Electroporation was performed according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... U2OS cells were transfected with Cas9 and guide RNA expression plasmids using Amaxa electroporation as recommended by the manufacturer (Lonza). Cas9 expression plasmid was from the Church lab (Addgene 41815) ...
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bioRxiv - Biochemistry 2019Quote: ... 3×107 cells were transfected with 10 μg of NotI linearized plasmids using the AMAXA electroporator (Lonza, program X-001) as described [14] ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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bioRxiv - Microbiology 2019Quote: ... 10 ug of Cas9 plasmid was combined with up to 80 ug of amplicon and resuspended in 20 uL P3 solution (Lonza), before being transfected by the Amaxa 4D Nucleofector (Lonza ...
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bioRxiv - Genomics 2021Quote: ... two million H9 hESCs were co-electroporated with the appropriate knockin vector (5 μg) and plasmids encoding AAVS1-targeting TALENs (2 μg; addgene, 59025 and 59026) using an Amaxa 4D Nucleofector system (Lonza). Serial cell dilutions were then seeded in six-well plates in E8 supplemented with Y-27632 (10 μM) ...
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bioRxiv - Genomics 2020Quote: ... 5 million cells were transfected with 5 μg of DNA FAIRE-STARR library plasmid using the Amaxa Nucleofector kit V (Lonza). For each condition ...
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bioRxiv - Genomics 2020Quote: ... A2lox ESCs (a gift from Michael Kyba) were transduced with 20 μg of each plasmid using the 4D-Nucleofector (Lonza), mouse ES program and P3 primary cell kit ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μg of linearized plasmid containing wild-type or Ku70 3A cDNA was transfected using Amaxa nucleofector solution V (Lonza) and program T-020 ...
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bioRxiv - Bioengineering 2021Quote: ... 200,000 cells were electroporated with 1000 ng adRNA plasmid or 48 pmol of IVT RNA using the Amaxa SF cell Line 4D-Nucleofector X kit (Lonza) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Patients-derived iPS cells were electroporated with plasmids and single stranded DNA donor oligos using Human Stem Cell NucleofectorTM Kit 1 (Lonza). After 36 hours ...
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bioRxiv - Microbiology 2021Quote: ... Following excystation a CRISPR guide plasmid and repair template with 50 base pair homology arms were introduced via nucleofection using the AMAXA 4D Nucleofector (Lonza). Transfected sporozoites were then diluted in PBS and used to infect a cage of mice ...
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bioRxiv - Microbiology 2020Quote: ... 2019): The plasmid was linearized using PsiI-V2 and purified using phenol-chloroform precipitation and resuspended in P3 solution (Lonza). Successful linearization was confirmed using agarose-gel electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: ... were nucleofected with either PGK-mO-Dam or PGK-mO-Dam-Zfp266 plasmids using the P4 Primary Cell 4D-Nucleofector X Kit (Lonza). 5 replicates were performed in total ...
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bioRxiv - Neuroscience 2022Quote: ... of CLN3 iPSCs was electroporated with Cas9-sgRNA ribonucleoprotein (IDT) and 966 bp donor plasmid using Amaxa 4D Nucleofector (Lonza) with program CB 150 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5μg of selected plasmid (control, empty vector (EV) or containing guide RNAs using Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2×105 NSCs for essential gene expression were nucleofected with 750 ng each of the above-mentioned plasmids using SG Cell Line 4DNucleofector X Kit (Lonza), DN-100 program ...
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bioRxiv - Biochemistry 2022Quote: ... the plasmids mEmerald-JUP-C-14 and mEmerald-Beta-Catenin-20 were transfected in 3T3-L1 pre-adipocytes by Nucleofection (Lonza). mEmerald-JUP-C-14 (Addgene plasmid # 54132 ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Cell Biology 2021Quote: ... or mutant) plasmids (4µg) were introduced to 4×106 dissociated neurons using Amaxa rat nucleofector kit (Lonza Bioscience, VPG-1003). Amaxa-nucleofected neurons plated at 500,000 cells per 35mm poly-D-lysine-coated glass bottom dish (WillCo Wells ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution in a 25-μL electroporation cuvette (Lonza). Cells were electroporated according to the manufacturer’s protocol.
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bioRxiv - Genetics 2019Quote: For gene editing 2×106 ESCs were transfected with 2 μg Cas9-GFP-guide plasmid and 5 μl of 100 μM ssODN repair templates (180 bp, IDT ultrameres) using electroporation (Nucleofector, Lonza). Cells were resuspended in 100 μl of P3 solution (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... karyotypically diploid H7 subline was transfected with pCAG-H2B-RFP plasmid (a kind gift from Dr Jie Na, Tsinghua University, Beijing) using the 4D nucleofector (Lonza) in the “P3 Primary Cell solution” as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Bacmids were prepared by transposition of these plasmids and were used to transfect Sf9 cells in Insect Express media (Lonza) before viral amplification in the same cell line using standard techniques ...
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bioRxiv - Molecular Biology 2021Quote: ... 10,000,000 lymphoblastoid cells were nucleofected with 60 µg of RTW3027 and 20 µg of plasmid expressing gRNA and mCherry using Cell Line NucleofectorTM Kit V (Lonza) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: 5×105 HAP1 cells were transiently transfected with 300 ng of plasmid using the SE cell line 4D-Nucleofector kit S (Lonza), on a 4D nucleofection unit (Lonza ...
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bioRxiv - Genetics 2021Quote: ... were transfected with either KMT2D CRISPR-Cas9 vector or CRISPR-Cas9 empty vector together with GFP plasmid using P3 Primary Cell 4D-Nucleofector X kit (V4XP-3024, Lonza). Three days after transfection ...
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bioRxiv - Biophysics 2020Quote: ... The cell pellet was suspended in 100 μL electroporation medium and electroporated for either GFP-Nrx1β or NLG1-mCherry plasmids with the Amaxa Nucleofector system (Lonza), using 500,000 cells per cuvette and 3 μg DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 2×106 ESCs were nucleofected with Cas9-GFP plasmids and a repair template where applicable using 4D-Nucleofector Core Unit (Lonza), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Percoll-purified57 parasites at late schizont stage were transfected with 50 µg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described65 ...
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bioRxiv - Immunology 2021Quote: ... the vector backbone used for generation of this plasmid is a kind gift from Ulrich Wissenbach (Saarland University) who previously modified the AMAXA vector (Lonza) by replacing the sequence encoding GFP with a linker sequence encoding a multiple cloning site (pMAX) ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 NALM6 target cells were transfected with 1 μg plasmid using the Nucleofector 4D (SF cell line Kit, program CV-104, Lonza) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... CD3/CD28 beads were removed 48 hours after stimulation and 5 × 106 CTLs were electroporated with 2 μg plasmid using 4D-Nucleofector (Lonza). Medium was changed 6 hours after nucleofection and transfected cells were used 24-36 hours after electroporation ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 μg of plasmid DNA was introduced to 4×106 neurons using an AMAXA nucleofection kit (VPG-1001, VPG-1003; Lonza). AMAXA-nucleofected cells were plated in 35mm glass bottom imaging dishes ...
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bioRxiv - Developmental Biology 2022Quote: 3 × 105 cells in 20 µL transfection buffer were mixed with 1µg plasmids and electroporated with a 4D nucleofector (Lonza Bioscience) following the preset protocol for K562 ...
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bioRxiv - Microbiology 2022Quote: ... ID) were excysted and transfected with 10 ug each of DiCre and floxed plasmids using an Amaxa 4D Electroporator (Lonza). The transfected parasites were diluted in HCT-8 infection medium and divided equally to infect three 24 well plates of sub-confluent HCT-8 cells ...
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bioRxiv - Neuroscience 2022Quote: Neurons were nucleofected with identified plasmids prior to plating at 0 DIV using Amaxa Rat Neuron Nucleofector kit (DGP-1003; Lonza) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Two million of MNCs were transfected with 10 μg of plasmid (5:1 ratio pEB-C5:pEBTg) as instructed by the Lonza CD34+ nucleofector kit (VPA-1003, Lonza) and Amaxa nucleofector (program T-016 ...
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bioRxiv - Microbiology 2023Quote: Constructed plasmids encoding AS1-S were introduced into MDBK cells using Amaxa cell line Nucleofector kit R (Lonza, Kanagawa, Japan) with Amaxa Nucleofector II system in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 107 epimastigotes in 0.5 mL were incubated with 30 μg of the plasmids in the buffer as described [101] and pulsed twice with the AMAXA Nucleofactor II electroporator (Lonza) X-014 program ...
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bioRxiv - Cell Biology 2023Quote: ... HCT116 cells were co-transfected with the pX330 plasmid containing the single gRNA target sequence and two donor DNA plasmids harboring puromycin or neomycin resistance cassette using a 4D Nucleofector (Lonza). After double selection with puromycin and G418 (geneticin ...
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bioRxiv - Cancer Biology 2023Quote: ... 2μgs of the PARP1-sgRNAR-eGFP plasmid was nucleofected into the U2OS PARP1-/-and OVCAR8 PARP1-/-cell line using the Nucleofector (Lonza). Cells were selected in 2μg/mL G418 for 10 days and GFP-positive cells were sorted and used for subsequent experiments ...
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bioRxiv - Genomics 2023Quote: ... Neurons were nucleofected with 10 µ total DNA plasmid DNA using the AD1 4D-Nucleofector Y kit (Lonza V4YP-1A24) with the ED158 (iCell GlutaNeurons ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were co-transfected with 2 µg of the CRISPR/Cas9 plasmid and 2 µg of ssODN (50 µM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... They were then reprogrammed to iPSCs by using a transient expression method (nucleofection) involving three plasmid vectors (MOS, MMK and GBX) under feeder-free/xeno-free culture on 4D Nucleofector (Lonza)11 ...