Labshake search
Citations for Lonza :
151 - 200 of 377 citations for Mouse MYCN shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: ... and the Mouse ES cell Nucleofector kit (Lonza, Cat No. VPH-1001). 36 hours after transfection the cells were selected by 2μg/ml puromycin for 48 hours ...
-
bioRxiv - Cancer Biology 2022Quote: Primary mouse embryonic fibroblasts (MEFs) were purchased from Lonza (M-FB-481). They were expanded in Advanced DMEM supplemented with 15% fetal bovine serum ...
-
bioRxiv - Bioengineering 2023Quote: ... hepatocytes were electroporated using 100 µL Mouse/Rat Hepatocyte Nucleofector solution (Lonza) and conditions ...
-
bioRxiv - Neuroscience 2023Quote: ... and the mouse neuron Nucleofector® Kit (Cat Number: VPG-1003, Lonza), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant plasmids were verified by Sanger sequencing and electroporated into E14 cells using the Nucleofector II platform (Lonza) (program A-013) ...
-
bioRxiv - Immunology 2022Quote: ... 2×106 B cells were then nucleofected with 2 μg of plasmid DNA using Nucleofector Kit V (Lonza) and rested for at least 16-24 hours using complete media containing 5 ng/ml BAFF and lacking LPS ...
-
bioRxiv - Bioengineering 2022Quote: ... Transfection efficiency was assessed by including a control plasmid encoding for constitutive GFP (pmax-GFP, Lonza, #V4YP-1A24). Media was changed within 7-8 hours after transfection to E8 flex and ...
-
bioRxiv - Immunology 2019Quote: ... gRNA and Cas9 containing plasmids were introduced to prostate epithelial cells using the basic nucleofector kit (Amaxa, Lonza) following the manufacturer’s instructions for primary mammalian epithelial cells (program W001) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl OptiMEM were mixed with 0.75 µl Lipofectamine and then added to 25 µl OptiMEM containing 0.1 µg GFP pMAX plasmid (Lonza) and 1 µl P3000 reagent ...
-
bioRxiv - Cell Biology 2021Quote: ... at 0.1-1 μg/mL or as a control a GFP plasmid (PmaxGFP, Lonza, Cat. No. D-00059) using Lipofectamine 3000 (Invitrogen ...
-
bioRxiv - Genomics 2022Quote: ... Wild type K562 cells were transfected with both the donor and guide plasmids by nucleofection (Lonza, program T016). Cells were selected 3 days after transfection with puromycin (2µg/mL ...
-
bioRxiv - Cancer Biology 2020Quote: Two million cells were harvested and transfected with or without 1.5μg of BRAFV600E plasmid (obtained from Dr. Dan Gough) using AMAXA Nucleofector (Lonza), according to supplier’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Plasmids were electroporated in ESC using the Lonza NucleofectorTM 2B Device using program A-23 (Lonza, AAB-1001). 10 µg of plasmids were mixed with 5 millions ESC in buffer “Mouse ES cell nucleofector kit (Lonza ...
-
bioRxiv - Genomics 2021Quote: ... K562 cells were grown to 1×106 and transfected with 1,000ng of gRNA plasmid and 3,000 ng of pCMV_AncBE4max_P2A_GFP (Amaxa® Cell Line Nucleofector® Kit V, Lonza). K562 cells were cultured for an additional 48 hours then single clones of green cells were isolated using flow cytometry (Aria II) ...
-
bioRxiv - Cell Biology 2022Quote: ... expression constructs were transfected into cells using the Amaxa™ 4D-Nucleofector (10μg plasmid cDNA; Lonza, Basel, Switzerland) or JetPrime (2μg plasmid cDNA ...
-
bioRxiv - Immunology 2023Quote: ... and nucleofected with 5 μg of a plasmid using program X-001 in the Nucleofector IIb device (Lonza) according to the manufacturer’s procedure ...
-
bioRxiv - Cell Biology 2023Quote: For immunoprecipitations performed on THP-1 cells were first transfected with 2 μg of empty vector pCDNA 3.1 or plasmid containing 3x FLAG_Vamp-3 using the Amaxa 4D nucleoporator system (Lonza) with the Amaxa SG Cell line kit and program FF-100 and following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Around five million WT serum-grown ESCs were transfected with 1μg of plasmids using Amaxa 4d Nucleofector (Lonza) and plated at a low density ...
-
bioRxiv - Genomics 2019Quote: The input library plasmids were electroporated into the K562 cells with Cell Line Nucleofector Kit V (Lonza, VCA-1003). For each electroporation ...
-
bioRxiv - Neuroscience 2019Quote: ... 8 × 105 cells were co-transfected with 3 µg plasmid-sgRNA construct and ssODN (0.6 µM final concentration) with Amaxa Human Stem Cell Nucleofector Kit 1 (Lonza) and program A-023 for the Amaxa Nucleofector Device II ...
-
bioRxiv - Genetics 2019Quote: ... Hela cells were electroporated with plasmids using Ingenio electroporation kit (SopaChem) and the Nucleofector™ II/2b device (Lonza). Cells were harvested 24h post-electroporation ...
-
bioRxiv - Genomics 2020Quote: ... 1 million U2OS cells were transfected using the Nucleofector 2b with 2 μg Plasmid DNA using kit V (Lonza) according the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: ... pCAGIG-hA3A-BE3 plasmid was delivered into cells using SF Cell Line 4D-Nucleofector X Kit (Lonza, #V4XC-2032) using programme CA-137 ...
-
bioRxiv - Cell Biology 2021Quote: Vero cells were transfected with 2 µg plasmid DNA in the Amaxa Nucleofector solution R (Lonza Bioscience, Rockville, MD), following the manufacturer’s instructions with program V-01 ...
-
bioRxiv - Cell Biology 2022Quote: ... 300 pmol of HDR oligonucleotide was electroporated into one million HEK293 Flp-In T-Rex cells along with 2.5 μg each of pSpCas9(BB)-2A-Puro and BiP gRNA plasmid (Amaxa kit R, program A-24; Lonza). Immediately after electroporation ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 1 million cells were transfected with 2μg of donor vector and 2μg of each AAVS1 ZFN expression plasmids using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza). Cells were seeded in E8 medium supplemented with 10µM ROCK Inhibitor Y-27632 (Selleckchem) ...
-
bioRxiv - Bioengineering 2019Quote: K562 cells were transfected with two gRNAs targeting exon 1 and 3 of NGLY1 and Cas9 plasmid (lentiCas9-Blast) by Nucleofection according to the manufacturer’s protocol (Nucleofector, Lonza). LentiGuide-Puro (Addgene plasmid #52963 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1,000,000 cells were nucleofected with 3.33 µg of SQT1665 and 1.66 µg of plasmid expressing gRNA using SE Cell Line 4D-NucleofectorTM X Kit L (Lonza) according to manufacturer’s protocol ...
-
Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: SiRNAs or overexpressing plasmids were transfected into day 4 differentiated BAT1 brown adipocytes using Amaxa Nucleofector II Electroporator (Lonza) with an Amaxa cell line nucleofector kit L according to the manufacturer’s instructions (Lonza ...
-
bioRxiv - Immunology 2021Quote: ... 2×106 cells were nucleofected with 2μg of pcDNA3.1+ plasmid per reaction (Lonza-AMAXA program X-001, Nucleofector 2b). 24 hours after transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... 1983) parasites at late schizont stage were transfected with 50 μg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described (Moon et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG, G-013 program; Lonza) before plating and maintained for 48 h.
-
bioRxiv - Microbiology 2022Quote: The resulting plasmid pool was linearised and transfected into 107 PRUΔHXGPRT tachyzoites using the Amaxa 4D Nucleofector system (Lonza) with buffer P3 and pulse code EO-115 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 160,000 cells per condition were nucleofected with the RNPs and 0.1 μg/μl of a pCAG-GFP plasmid using the 4D-Nucleofector by Lonza (CB150 pulse ...
-
bioRxiv - Developmental Biology 2023Quote: ... containing 10 μg of the plasmid DNA and transfected using the program DS-112 of the 4D-nucleofector (Lonza). NSCs were harvested 48 hours post-nucleofection ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2 μg of assembled pGL3 plasmid were electroporated into 2.5×105 AIC-H9-hESCs 35 by 4D-Nucleofector (Lonza) using P3 Primary Cell 4D-Nucleofector X kit (Lonza) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 x 105 cells were transfected with the pU6-Sp-pegRNA-HEK3-insCTT plasmid (1,000 ng) using the SF Cell Line 96-well Nucleofector Kit (Lonza). Program code was FF-120.
-
bioRxiv - Cancer Biology 2023Quote: ... 2×10E6 ME-1 cells were nucleofected with CRISPR/Cas9 plasmids (2 μg each) using Nucleofector Technology (Lonza Biologics) with the program X-01 and Amaxa Cell Line Nucleofector Kit V ...
-
bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated Rb KO cell lines were electroporated with 3 µg of either pE2F-TA-luc or pRb-TA-luc and 0.3 ug renilla luciferase plasmid on an Amaxa Nucleofector 2b (Lonza). Following transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected into human U2OS cells (ATCC HTB-96) by Amaxa electroporation with Nucleofector™ Kit V (Lonza) and plated in 6 well plates containing glass slides.
-
Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: A total of 3-4µg of plasmid DNA was electroporated into 3-4x106 early passage ES cells using the Amaxa Nucleofector II (Lonza) programme A-023 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X S kit (Lonza) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... Cell pellets were resuspended in nucleofector buffer (Amaxa Mouse Macrophage Nucleofector Kit (Lonza)) and 2ug of siRNA was added to each tube (Dharmacon) ...
-
bioRxiv - Developmental Biology 2020Quote: ... ESCs were electroporated using Nucleofector II (Amaxa) and mouse ESC Nucleofector kit (Lonza). Afterwards ...
-
bioRxiv - Microbiology 2021Quote: Mouse femurs were removed and flushed with Hank’s Buffered Salt Solution (HBSS-Lonza) as described [23] ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were transfected with the Amaxa Mouse Neuron Nucleofector Kit (Lonza, VPG-1001) using the Amaxa Nucleofector II device (Lonza) ...
-
bioRxiv - Bioengineering 2023Quote: Freshly isolated primary mouse hepatocytes were electroporated with a 2b Nucleofector device (Lonza) using program T-028 as previously described (34) ...
-
bioRxiv - Genetics 2023Quote: ... The Amaxa Mouse Embryonic Stem Cell Nucleofector Kit was used (Lonza, VPH-1001), program A-24 ...
-
bioRxiv - Genomics 2021Quote: ... 1.6×105 cells were suspended in P3 Primary Cell Solution with 50pmol Cas9 protein (St. Jude Protein Production Core) and 150pmol sgRNA (Synthego) with pMaxGFP plasmid at 200ng/ul (Lonza) in a total volume of 20ul with program DS130 ...
-
bioRxiv - Genomics 2021Quote: ... and 50 pmole of spCas9 protein (St. Jude Protein Production Core) with pMaxGFP plasmid as a transfection control at 200ng/ul (Lonza) using the 4D-Nucleofector™ X-unit (Lonza ...