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Citations for Lonza :
4251 - 4300 of 4418 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... freshly isolated neurons (5 million cells per nucleofection reaction) were transfected using the 4D-Nucleofector™ X Unit and the P3 Primary Cell 4D Nucleofector X kit (Lonza, Switzerland), as indicated ...
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bioRxiv - Cell Biology 2020Quote: ... The pPBbsr-based FRET biosensor and pCMV-mPBase (neo-) encoding the piggyBac transposase were co-transfected into MDCK cells using an Amaxa nucleofector system (Lonza, Basel, Switzerland) at a ratio of 4:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... AsPC-1 and CAPAN-1 pellets were suspended in Lonza transfection reagent (SE Cell Line 4D-Nucleofector™ X Kit L, Lonza, Germany) when the Mirus transfection reagent was finished ...
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bioRxiv - Cell Biology 2021Quote: ... 500μg of gRNA and 500μg of donor plasmids were electroporated in 1×106 mESC using P3 Primary Cell 96-well Nucleofector™ Kit (Lonza, PBP3-22500) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Genomics 2022Quote: ... 5 million cells were transfected with 10ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit T (Lonza), program X-001 ...
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bioRxiv - Molecular Biology 2022Quote: ... Electroporation of 5×106 HL-60 cells was performed with AIO-GFP HAX1 LCRC1 and AIO-GFP HAX1 LCRC2 using CLB-Transfection™ Kit (Lonza, Austria) and CLB-Transfection™ System (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 million NK cells were resuspended in 20 μl Amaxa 4D nucleofector master mix (82% P3 + 18% supplement 1) (Lonza, V4XP-3032), then mixed with Cas12a RNPs for electroporation using program CM137 ...
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bioRxiv - Biophysics 2022Quote: ... 106 U2OS cells were transfected with 1 μg of px330 plasmid and 1 μg of the HDR donor plasmid using the Nucleofector 2b device and cell line nucleofector kit V (Lonza, VCA-1003) per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 5×106 cells were mixed with RNPs and transferred without forming bubbles to a 16-well Nucleocuvette© Strip (Lonza, V4XP-3032). Unless otherwise stated ...
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bioRxiv - Molecular Biology 2021Quote: ... Spodoptera frugiperda Sf9 insect cells (kindly provided by P. Cejka) were grown at 27°C in serum free Insect-XPRESS medium (Lonza, BELN12-730Q).
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bioRxiv - Microbiology 2020Quote: ... ALI cultures were established from enzymatically dissociated human sinonasal epithelial cells (HSEC) as previously described (85, 86) and grown to confluence with bronchial epithelial basal medium (BEBM; Lonza, Alpharetta, GA) supplemented with BEGM Singlequots (Lonza) ...
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bioRxiv - Molecular Biology 2020Quote: ... Point mutations were introduced into CJ7 WT using CRISPR RNP transfected into cells with the Amaxa mouse ES kit (Lonza VPH-1001). 1×106 cells were transfected with the RNP containing two separate guide RNAs a single stranded donor oligo and a linearized puromycin resistance gene ...
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bioRxiv - Immunology 2020Quote: ... 106 cells per condition were nucleofected with 4 µg of DNA using the Amaxa Nucleofector II (Lonza, Kit R; program R-024). Nucleofected cells were allowed to recover for 24-48 hours before sorting for GFP positivity and lack of CD56 expression.
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bioRxiv - Neuroscience 2021Quote: Dissociated neurosphere cells were electroporated with either CFP-IRES or Safb-IRES-CFP vector (4µg) using a 4D-Nucleofector (Lonza, program DS-112) in 16-well stripes (500,000 cells/well ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were plated alone or with macrophages (BAC1.2F5) or HUVEC (Lonza, cat# CC-2517) at a 1:5 ratio (5,000 tumor cells and 25,000 macrophages or HUVEC ...
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bioRxiv - Systems Biology 2022Quote: ... All cells were grown at 37°C in 5% CO2 and regularly checked for mycoplasma (MycoAlert mycoplasma detection kit, Lonza, Basel, Switzerland). Identity of all cell lines was confirmed by STR analysis (Leibniz Institute DSMZ GmbH ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were authenticated as recommended by AACR (http://aacrjournals.org/content/cell-line-authentication-information) and tested for the absence of mycoplasma using MycoAlert® (Lonza, Basel, Switzerland) or MycoFluor (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... and cultured in endothelial cell basal medium-2 (EBM-2) supplied with EGM™-2 BulletKit™ (catalogue no. CC-5035; Lonza). HEK293A cells were purchased from Invitrogen (catalog no ...
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Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studiesbioRxiv - Bioengineering 2019Quote: ... Madin-Darby Canine Kidney strain II (MDCKII) cells were transfected with Life-Act GFP using the Amaxa Biosystem Nucleofector II system (Lonza, VCA-1005) (Gift from Jens Moller ...
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bioRxiv - Cancer Biology 2019Quote: ... THP1 and NB4 cells (ATCC: CCL-243, TIB-202 and DSMZ: ACC 207) were maintained in RPMI 1640 (BioWhittaker, Lonza, Verviers, Belgium), 10% fetal bovine serum (FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... Dharmacon) or non-targeting control pools (Horizon, Dharmacon) were introduced to H7 ESCs and HeLa cells using 4D-Nucleofector™ (Lonza™) electroporation as per manufacturer instructions for each cell line (ESC ...
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bioRxiv - Molecular Biology 2020Quote: ... The dissociated cell suspension was cultured on rat tail collagen type I-coated dishes at 37 °C in 5% CO2 using Small Airway Epithelial Cell Growth Medium (SAGM; basal medium plus growth supplements, Lonza, CC-3118) containing 1% AA ...
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bioRxiv - Neuroscience 2021Quote: ... Single-cell suspension of hiPSCs was nucleofected with 5 μg of the generated SpCas9-sgRNA plasmid using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, #V4XP-3024) in combination with the 4D Nucleofector Unit X (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... All cell lines were routinely tested for the absence of mycoplasma using the MycoAlert Plus Mycoplasma Detection Kit from Lonza (Walkersville, MD).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were maintained in RPMI 1640 medium supplemented with 2 mM Glutamine and 10% (v/v) heat-inactivated FBS (Lonza Group Ltd.) containing 100µg streptomycin ml−1 and 100 U penicillin ml−1 and passaged as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
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bioRxiv - Immunology 2020Quote: ... Transfection of primary T cells with Cas9 RNP complexes and TCRβα HDR templates was performed 3-4 days following activation using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... were isolated by Ficoll density gradient centrifugation as described previously [22] and seeded at a density of 1×106 cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2019Quote: Primary human normal airway tracheobronchial epithelial cells from de-identified donors (NHBE) (sex: male and female) were provided by Lonza (Walkersville, MD) and grown in BEM media supplemented with the BEGM bullet kit (Lonza) ...
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bioRxiv - Microbiology 2019Quote: ... All parasite strains and host cell lines were routinely tested for Mycoplasma contamination with the MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland) and found to be negative.
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed 1x in RT PBS and transfected with ∼15μg of DNA plasmid using Amaxa nucleofector 2b (Lonza, program O-005). After transfection ...
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bioRxiv - Genomics 2021Quote: ... All the cells were grown with 5% CO2 at 37°C and verified mycoplasma free using the MycoAlert Mycoplasma Detection Kit (Lonza, LT07-218).
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bioRxiv - Biochemistry 2021Quote: ... 105 cells were mixed with a plasmid vector (AsCas12a, n-SpCas9 (D10A, H840A)) and 20 μl of electroporation buffer (Lonza, V4XC-2032) and were nucleofected according to the manufacturer’s instructions (program ...
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bioRxiv - Molecular Biology 2021Quote: ... Myoblasts derived from an adult DM1 patient biopsy (DM-05 (Xia et al., 2013)) were cultured in SkGM-2 skeletal muscle cell growth medium (Lonza, #CC-3245).
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bioRxiv - Cell Biology 2020Quote: ... NIH 3T3 and immortalized MVD7 cells were cultured at 37°C and 5% CO2 in high glucose DMEM culture medium (Lonza, Cologne, Germany) supplemented with 10% FBS (Biowest) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with pcDNA3.1 empty vector or pcDNA3.1 containing the human coding sequence of the VGLL2-NCOA2 fusion using the AMAXA cell line Nucleofection kit V (Lonza #VCA-1003;) and were selected in growth media containing 1μg/ml concentration of G418 (ThermoFisher #10131035) ...
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bioRxiv - Immunology 2020Quote: ... Electroporation was then performed by transferring 20 μL of T cell-RNP mixture into the transfection vessel and using the Lonza transfection program EH:115 (4D-Nucleofector, Lonza, Walkersville, MD). Electroporated T cells were then allowed to recover for overnight in RPMI media supplemented with 20% FBS and 1% glutamax and in the presence of IL-7 and IL-15 cytokines at 10ng/mL and 5ng/mL respectively.
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bioRxiv - Biophysics 2021Quote: ... All cell lines were regularly tested for mycoplasma infection and were found to be negative (MycoAlert Plus Detection Kit, Lonza, LT07-710).
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bioRxiv - Immunology 2021Quote: ... Mononuclear cells were isolated with Ficoll-hypaque (γ = 1.077, GE Health) and red blood cells were lysed using ammonium-chloride-potassium (ACK) lysis buffer (Lonza, MD, USA). The remaining mononuclear cells were seeded in 150 x15 mm style non-tissue culture-treated petri dishes or non-tissue culture-treated 24-well plates at 1 ×106 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... 2 million L1.2 cells were transfected with 2 µg of plasmid using the SG Cell Line transfection kit and a 4D-Nucleofector X (both from Lonza, Walkersville, MD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...
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bioRxiv - Microbiology 2021Quote: ... All cell lines were routinely tested to confirm absence of mycoplasma using the MycoAlert Plus Mycoplasma Detection Kit from Lonza (Walkersville, MD).
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bioRxiv - Genomics 2021Quote: ... transfection of primary T cells with Cas9 RNP complexes and GSH1/GSH2-mRuby HDR templates was performed using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Single iPSCs were then nucleofected with the pX459v2 plasmid coding for the Cas9 protein and the sgRNA using P3 primary Cell 4D nucleofector kit (Lonza, V4XP-302). After nucleofection cells were plated on a 6-well plate in E8 flex medium supplemented with RevitaCell ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells lines were maintained in humidified 37°C the incubator with 5% CO2 and routinely tested for mycoplasma contamination (Lonza #LT07-118).
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bioRxiv - Developmental Biology 2022Quote: ... D-001206-14-05 5 nmol) and nucleofected into OPCs using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were co-transfected with 1 ug of firefly Luciferase vector and 0.1 ug Renilla luciferase vector by nucleofection with Lonza Cell Line Nucleofector® Kit V (Lonza, #VVCA-1003) and the T-020 program in the Amaxa Nucleofector II device ...
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bioRxiv - Bioengineering 2022Quote: Cell cultures were monitored for the presence of mycoplasma contamination using the MycoAlert® Mycoplasma Detection Kit (Lonza #LT07-318, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 105 single cells were resuspended with Lonza P3 Primary Cell Nucleofector® Solution and mixed with the pre-formed RNP complexes and transferred to a Nucleocuvette™ (Lonza). Nucleofection was performed using program EO-115 on the 4D Nucleofector™ X unit (Lonza) ...