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Citations for Lonza :
351 - 400 of 546 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells underwent nucleofection using a Lonza P3 Primary Cell 4D Nucleofector X Kit S (Cat. # V4XP-3032) and a Lonza 4D system (Lonza 4D Core Unit Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Schizonts were transfected by electroporation using the Lonza 4D Nucleofector System according to the pulse program FI-115 with the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). After transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... CD34+ HSPCs (2x105 cells/condition) were transfected with RNP complexes using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza) and the CA137 program (Nucleofector 4D ...
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bioRxiv - Bioengineering 2024Quote: 3·106 BRPKp110 cells were resuspended in 20 µl primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X kit S (32 RCT, V4XP-4032; Lonza). Cells were mixed and incubated with 15 µl RNP at room temperature for 2 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... and resuspended at a concentration of 2.5 x 105 cells in 20 μl of pre-mixed P3 Primary Cell 4D-Nucleofector solution (P3 Primary Cell 4D-Nucleofector X Kit S, Cat: V4XP-3032; Lonza). Cells were combined with the RNP/HDR plasmid solution and transferred into individual wells of a 16-well Nucelocuvette Strip (Lonza) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were resolved on 3% Metaphor agarose gel (Lonza), and DNA fragments of sizes approximately 150-200 nt were isolated from the gel using Qiagen’s Gel Extraction Kit (Figure 1D) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were maintained in FGM-3 Medium (CC-4526, Lonza) and passaged at 80% confluence.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CF patient# 3 (#28388-0000450918, Lonza, male, 25 years-old) and four non-CF donors with no pathology reported ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in 3% agarose (SeaKem LE Agarose, Lonza). 150 μm thick vibratome sections (VT1000S vibratome ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in 3% agarose (SeaKem LE Agarose, Lonza) and vibratome-sectioned (VT1000S vibratome ...
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bioRxiv - Systems Biology 2024Quote: ... and finally resuspended in a cold SSC cocktail (3× Lonza AccuGENE SSC ...
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bioRxiv - Microbiology 2022Quote: Sterile molten top NA (3 mL; 0.2 % SeaPlaque Agarose, Lonza) supplemented with CaCl2 and MgCl2 (both at 5 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to the well of a 16-well Nucleocuvette™ strip (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were electroporated on a 4D-NucleofectorTM X Unit (Lonza ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended with 10µL of primary cell nucleofection solution (P3 primary Cell 4D-Nucleofector X kit S (Lonza, # V4XP-3032), and mixed with RNP complexes ...
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bioRxiv - Immunology 2022Quote: ... were delivered into the CD4+ Human T cells by using Amaxa 4D-Nucleofector System and P2 Primary Cell 4D-Nucleofector® X Kit S (Lonza) according to manufacturer’s instructions using the pulse code EH100 ...
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bioRxiv - Immunology 2023Quote: ... Electroporation was performed with sgRNA/Cas9 RNP complexes using Alt-R Sp Cas9 Nuclease V3 (IDT) and using the 4D-Nucleofector with P3 Primary Cell 4D Nucleofector X Kit S (Lonza Bioscience).
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bioRxiv - Cell Biology 2024Quote: ... 2 × 105 to 1 x 106 HSPCs per condition were electroporated two days after thawing with 3.0 μg of the ABE-encoding mRNA and 3.2 μg of the synthetic sgRNA using the P3 primary cell 4D-Nucleofector X Kit S (Lonza, Basel, Switzerland) and the CA-137 program (Nucleofector 4D) ...
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bioRxiv - Immunology 2024Quote: ... Naive DOCK2-GFP OT-I T cells were resuspended in P4 Primary Cell 4D-Nucleofector™ X Kit S (V4XP-4032, Lonza) buffer solution at a cell concentration of 5 x 106 cells in 20 µL ...
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bioRxiv - Immunology 2021Quote: Calu-3 epithelial lung cancer cells were cultured in DMEM (Lonza) supplemented with 10% heat inactivated fetal calf serum ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were size-selected using 3% NuSieve agarose gels (Lonza) followed by gel extraction using QIAEX II reagents (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: ... Adipose-derived stem cells (Passage 3-5) (Lonza, Walkersville, MD, USA) were cultured in a basal medium consisting of DMEM/F12 ...
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bioRxiv - Cell Biology 2022Quote: ... the PBS was removed and 3 mL of SkBM-2 (Lonza) media was added to each chamber ...
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bioRxiv - Microbiology 2022Quote: ... 3 ml of lung digestion buffer containing HBSS (Lonza, BE10-508F), 5% FBS ...
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bioRxiv - Microbiology 2022Quote: ... a 3 ml of DMEM containing 0.5% SeaKem ME agarose (Lonza), 5% (v/v ...
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Neuroscience 2024Quote: ... Retinal tissues were embedded in 3% low-melting agarose (#50100, Lonza) within a plastic cubic mould ...
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bioRxiv - Cell Biology 2020Quote: ... and the GFP-K17ΔNLS (Hobbs et al., 2015) were nucleofected into HeLa cells using SE Cell Line 4D X nucleofector Kit S (Lonza #V4XC-1032) with setting DS-138 ...
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bioRxiv - Immunology 2021Quote: The Tap2-deficient T lymphoma cell line RMA-S (provided by Professor Klas Kärre, Karolinska Institutet) was cultured in RPMI-1640 medium supplemented with L-glutamine (Lonza, catalogue# 12-702F), penicillin-streptomycin (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 100,000 MCF10a cells was nucleofected with program DS-138 using an Amaxa 4D-Nucleofector X using the SE Cell Line 4D X Kit S 32 RCT (Lonza V4XC-1032). Reactions were split between two 24-well plates and grown in complete media three days ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were resuspended in 20 µl nucleofection buffer (16.4 µl Nucleofector® Solution + 3.6 µl Supplement) provided in P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032). After the addition of 3 µl RNP and 0.5 µl of Alt-R Cas9 Electroporation Enhancer (IDT ...
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bioRxiv - Genomics 2023Quote: ... were washed twice with PBS and resuspended in a solution containing a 4.5:5 mixture of nucleofection buffer (Buffer SE, Lonza Lot #: S-09279) and supplement (Lonza ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... and immediately transfected by Nucleofection into HT-1080 cells using the Amaxa SF Cell Line 4D-Nucleofector X kit S (Lonza, PBC2-00675) and either program FF-113 (HT1080 ...
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bioRxiv - Bioengineering 2023Quote: ... 100,000 cells were resuspended in 20μl P3 reagent of the P3 Primary Cell 4D-Nucleofector® X Kit S (Lonza V4XP-3032). 1 μg total plasmid was used for a single nucleofection event and nucleofected by program EH-100 ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Genetics 2024Quote: ... 3x105 Daudi cells were nucleofected with 20 picomole of sgRNA-Cas9 complex and 100 picomole of DNA donor template using program CA137 of Amaxa 4D-Nucleofector and SF cell line kit S (Lonza, V4XC-2032). The edited single-cell clones were sorted into 96-well plate by BD Aria II sorter and expanded for 4 weeks ...
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bioRxiv - Genomics 2024Quote: ... 200,000 cells were nucleofected with either 500 ng pJK028A and 500 ng pJK029A or 1 μg pJKEB01 using the Lonza SF Cell Line 4D-Nucleofector X Kit S (Lonza, V4XC-2032) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and immediately transfected by Nucleofection into MCF-10A or HaCaT cells using the Amaxa SF Cell Line 4D-Nucleofector X kit S (Lonza, PBC2-00675) and the program DS-138 (MCF-10A and HaCaT ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 OVCAR8 landing pad cells were resuspended in 20 µl of nucleofector solution combined with supplement at 4.5:1 from the Amaxa SE Cell Line 4D-Nucleofector X Kit S (Lonza, Cat #V4XC-1032), before addition of 5 µl RNP complex and 1 µl of Electroporation enhancer ...
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bioRxiv - Cancer Biology 2024Quote: ... were separated on a PA-TBE gel and stained with GelStarTM Nucleic Acid Gel Stain (Lonza, 50535).
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bioRxiv - Immunology 2022Quote: ... The Cas9 protein and sgRNA were electroporated into the T cells by using Amaxa 4D-Nucleofector System and P4 Primary Cell 4D-Nucleofector® X Kit S (Lonza, V4XP-4032) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets of 1E6 cells were resuspended with 20ul supplemented nucleofector solution of the P3 Primary Cell 4D-nucleofector® X Kit S (Lonza, V4XP-3032) and 2ug plasmid (1ug/ul) ...
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bioRxiv - Immunology 2024Quote: ... MC38 Pdl1 KO cells were generated through CRISPR Cas9-gRNA RNP-directed deletion by using a Lonza 4D-Nucleofector and a SF Cell Line 4D-Nucleofector X Kit S (Lonza, cat. V4XC-2032). In brief ...
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bioRxiv - Immunology 2024Quote: ... The nucleofection was conducted using a Lonza 4D-Nucleofector and a P3 Primary Cell 4D-Nucleofector X Kit S (Lonza, cat. V4XP-3032) with the same protocol described earlier ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then additionally nucleofected with ribonuclease complex of ITGB2 sgRNA and Cas9 using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032) using 4D-Nucleofector (Lonza ...
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bioRxiv - Developmental Biology 2022Quote: ... 400,000 cells in 20 μl of P3 buffer were added to each RNP and electroporated using program 96-EA-104 on an Amaxa 4D-Nucleofector with a 96-well shuttle (Lonza # AAF-1003B/S). Immediately after electroporation ...
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bioRxiv - Bioengineering 2024Quote: Lonza electroporation was performed using manufacturer recommended protocols for the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza, Canada, Catalog # V4XP-3032). For each condition ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting mixture was electrophoresed in NuSieve 3:1 agarose (Lonza 50091), and fragments sized 150-350 bp were excised and then purified using a MinElute Gel Extraction kit (QIAGEN 28604) ...
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bioRxiv - Neuroscience 2021Quote: ... The behavioral assay consisted of a 3% agarose (SeaKem LE Agarose, Lonza) surface coating (50 mL ...
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bioRxiv - Bioengineering 2020Quote: ... passage 3-5 AFC were dissociated and resuspended in EGM-2 (Lonza) at 4×105 cells/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... as previously described (3) and the Cell Line Nucleofector Kit R (Lonza). Briefly ...