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Citations for Lonza :
301 - 350 of 546 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Nucleofection was performed using the P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032), a 4D-nucleofector core unit ...
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bioRxiv - Cell Biology 2023Quote: ... and these cells were transferred to a cuvette (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) for nucleofection in a 4D-Nucleofector (Lonza ...
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bioRxiv - Bioengineering 2023Quote: Nucleofection in T cells were performed using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza) according to the manufacturer’s instruction with minor modifications ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... cells were transfected using Nucleofection with an Amaxa SF Cell Line 4D-Nucleofector X kit S (Lonza) and program FF-113 (HT1080 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza-BioResearch, #: V4XP-3032) and Amaxa 4D- Nucleofector (Lonza-BioResearch) ...
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bioRxiv - Neuroscience 2023Quote: Larvae were anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate salt (Tricane, (E10521, Ethyl 3-aminobenzoate methanesulfonate) and mounted in 1% low melting point agarose (50100, Lonza) in a 60 mm x 15 mm petri dish ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... or either of two different gRNAs targeting exon 3 coding sequences of human TET2 (TET2-gRNA #1: 5’-TGGAGAAAGACGTAACTTC-3’ and TET2-gRNA #2: 5’-TCTGCCCTGAGGTATGCGAT-3’) were transfected with Nucleofector (Lonza). After transduction ...
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8%) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8% ...
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bioRxiv - Microbiology 2023Quote: ... and 3 ml of ACK lysis buffer (Lonza) was added to the cells and incubated for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 3% MetaPhorTM Agarose gels (Cat. No. 50181, Lonza) were used ...
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bioRxiv - Neuroscience 2024Quote: ... were nucleofected into 3 million cells by LONZA 4D-Nucleofector using program CB-150 ...
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bioRxiv - Immunology 2021Quote: ... The P14 cell/Cas9/RNP mixes were transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Immunology 2022Quote: ... 1 x 10^6 purified naive T-cells were resuspended in 15µL buffer P3 + 5µL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7µL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% penicillin-streptomycin (P/S: 100 U/ml penicillin and 100 U/ml streptomycin, Lonza, Basel, Switzerland) in 15 cm cell culture plates (Nunc ...
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bioRxiv - Neuroscience 2024Quote: ... we used the 4D-Nucleofector system and Amaxa P3 primary Cell 4D-Nucleofector X Kit S from Lonza. The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza) ...
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bioRxiv - Immunology 2024Quote: ... 16.4µl P3 and 3.6µl Supplement Reaction solutions from P3 Primary Cell 4D X Kit S (Lonza V4XP-3032) were added ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 × 106 HEK293T cells were transfected using SF Cell Line 4D-NucleofectorTM X Kit S (Lonza, V4XC-2012) with 7 µg of 5’ modified donor ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... and nucleofection was performed as per the manufacturer’s recommendations (Lonza, SG Cell Line 4D-Nucleofector X Kit S). Monoclonal isolation of the knockouts were performed by limiting dilution ...
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bioRxiv - Synthetic Biology 2024Quote: ... using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Neuroscience 2023Quote: ... CRISPR reagents were transfected into iPSCs using the P3 Primary Cell 4D Nucleofector™ X Kit S (Lonza), as previously described (Deneault et al. ...
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bioRxiv - Immunology 2024Quote: ... The P14 cell/Cas9/RNP mixes were transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Bioengineering 2024Quote: ... in a strip format using SF Cell Line 4D-Nucleofector™ X Kit S (Lonza, Cat# V4XC-2032). Purified PEs and CODEs were complexed with either pegRNA or cpegRNA to form RNP at 50 pmol protein ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10 mM N’-2-Hydroxyethylpiperazine-N’-2 ethanesulphonic acid (HEPES, Lonza), 2 mM Glutamax (Gibco) ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Developmental Biology 2020Quote: ... Cells were transfected using the P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza-BioResearch, Cat #: V4XP-3032) and Amaxa™ 4D-Nucleofector™ (Lonza-BioResearch) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 x 106 NSCs were mixed with 2.5 µg of corresponding DNA using Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) with CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: ... washed twice with PBS and 0.25×106 cells per condition resuspended in 20μL supplemented cell line solution (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were then transferred to the RNP solution ...
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bioRxiv - Immunology 2021Quote: ... using SF Cell line 4D-Nucleofector® X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X S kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were targeted by nucleofection using the SE Cell Line 4D-Nucleofector™ X Kit S (Lonza #V4XC-1032). Cells were allowed to recover from nucleofection before sib-selection-based subcloning to isolate pure clonal cultures ...
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bioRxiv - Neuroscience 2023Quote: ... All CRISPR reagents were electroporated into iPSCs using the P3 Primary Cell 4D Nucleofector™ X Kit S (Lonza), as previously described.(25 ...
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bioRxiv - Bioengineering 2022Quote: ... embryos were anesthetized by 30 mg/L tricaine-S (Western Chemical) and mounted in 2% low melting agarose (LONZA) for dorsal or lateral view in 35 mm Petri dishes with a glass bottom ...
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bioRxiv - Bioengineering 2024Quote: Nucleofection of effector plasmids was performed with SF Cell Line 4D-Nucleofector X Kit S following the manufacturer’s protocol (4D X-unit, FF-120 program, Lonza). PB Tet-On dCas9 K562 cell line (with CD81 guide array ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose (Lonza, # 50004, Rockland, ME, USA) and coronally sectioned (Leica VT1000S vibratome ...
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bioRxiv - Immunology 2021Quote: ... WT RAW264.7 cells were subjected to two rounds of nucleofection with Cas9-RNPs using the SF Cell Line 4D-Nucleofector X Kit S (Lonza) and the 4D Nucleofector apparatus precisely as specified by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 NSCs were mixed with 5 µg of corresponding DNA with the Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) and pulse was delivered using the CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... Twenty μl of cells in buffer were transferred to each well of a 16-well Nucleofection cuvette strip (4D-Nucleofector X kit S; Lonza). Three μl of RNP were gently dispensed to each well ...
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bioRxiv - Immunology 2022Quote: ... 1*106 purified naïve T-cells were resuspended in 15 μL buffer P3 + 5 μL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7 μL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Immunology 2022Quote: ... 2 million B cells were then washed with PBS and resuspended in 15 uL resuspension buffer (4D-Nuclcofector X Kit S, #V4XP-4032; Lonza). Cells in resuspension buffer (12.5ul ...
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bioRxiv - Systems Biology 2020Quote: 5×105 HAP1 cells were transiently transfected with 300 ng of plasmid using the SE cell line 4D-Nucleofector kit S (Lonza), on a 4D nucleofection unit (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: 3T3-L1 fibroblasts were maintained in DMEM supplemented with 10% newborn calf serum (NCS) and 1% penicillin/streptomycin (P/S) (all Lonza). Experiments were performed in six-well plates ...
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bioRxiv - Microbiology 2023Quote: Constructed plasmids encoding AS1-S were introduced into MDBK cells using Amaxa cell line Nucleofector kit R (Lonza, Kanagawa, Japan) with Amaxa Nucleofector II system in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 01F49i-N-B7 iPSCs were dissociated to single cells and 250,000 cells transfected with 25 μL of the prepared transfection mix containing 20 µL of nucleofection buffer (P3 Primary Cell 4D-NucleofectorTM X Kit S, Lonza), 5 µL of the RNP complex ...
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bioRxiv - Immunology 2023Quote: CRISPR–Cas9 gene knockout was performed by transient Cas9/gRNA (RNP) complex electroporation using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). On day 4 of culture ...
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bioRxiv - Genomics 2023Quote: ... 2.5e5 RPE1 CRISPRi cells were nucleofected with 3μM RNP complex using an SE Cell Line 4D X Kit S (Lonza Bioscience) on a 4D-Nucleofector (Lonza Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: Cas9-RNPs were transfected into cells by electroporation (SF Cell Line 4D-Nucleofector™ X Kit S (Lonza, #V4XC-2032)) ...
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bioRxiv - Synthetic Biology 2022Quote: ... T cells were resuspended in P3 buffer (0.75-1 x 106 cells per 18 μl P3) from the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). 10 μg/μl Alt-R S.p ...
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bioRxiv - Genomics 2023Quote: ... of the PCR products were analyzed by electrophoresis on a 2% Agarose S gel (Nippon gene) in 1× TBE buffer and stained with GelStar (Lonza). The remaining PCR product of the condition that displayed a single-band or near single-band product on the gel was double size-selected and purified using AMPure XP beads (Beckman coulter ...
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bioRxiv - Biophysics 2024Quote: ... 0.5 μg of F-tractin-mScarlet plasmid and 0.75 ug of Zyxin-mNeonGreen plasmid were mixed with solution SE from SE Cell Line 4D-Nucleofector S Kits (Lonza). 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).