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Citations for Lonza :
351 - 400 of 530 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human T cells were purified with EasySepTM beads and resuspended in Human T Cell Nucleofector™ Solution (Lonza). For each cuvette ...
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bioRxiv - Bioengineering 2023Quote: The DBM powders were decellularized in a solution of 0.05% trypsin with 0.02% ethylenediaminetetraacetic acid (trypsin/EDTA, Lonza) at 37 °C and 5% CO2 incubator under agitation for 24 hours (Figure 1c) ...
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bioRxiv - Genomics 2023Quote: 2 × 105 HCT116-Cas9 and iPSC-iCas9 were resuspended in 20 µl SE cell line nucleofection solution (Lonza) (HCT116-Cas9 ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNP mixture was added to cells resuspended in nucleofection solution along with 0.5 ug pmax-GFP (Lonza) and 1 uL electroporation enhancer (100 uM ...
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bioRxiv - Immunology 2024Quote: ... 16.4µl P3 and 3.6µl Supplement Reaction solutions from P3 Primary Cell 4D X Kit S (Lonza V4XP-3032) were added ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9 Nuclease V3 (IDT) and 3.0 μg donor fragment per 100 μL of Nucleofector™ Solution V (Lonza) using the Amaxa Nucleofector II (Lonza) ...
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bioRxiv - Genetics 2023Quote: ... 2−106 lymphoblastoid cells were suspended in 100 μL of Nucleofector C solution (VCA-1004, Lonza Cologne AG) with 0.6 μM RNAi and transfected with the Z-001 program according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended carefully in 20ul 4D-Nucleofector™ Solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza). Thereafter ...
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bioRxiv - Genetics 2024Quote: ... Daudi cells were then washed with PBS twice and resuspended in SF solution (Lonza, catalog number V4XC-2032), 300k cells per 20 µl solution ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected and incubated in prewarmed hypotonic solution containing 0.0375M KCl and 1:12 human serum (Lonza) for 20 min at 37°C ...
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bioRxiv - Immunology 2024Quote: Mouse primary CD4+ and CD8+ T cells were pelleted and resuspended in P3 Primary Cell Nucleofector Solution (Lonza) with 100 nM 2’3’-cGAMP to a density of 1 × 106 cells/mL ...
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bioRxiv - Cell Biology 2024Quote: ... treatments/exposures were aspirated from the apical compartment and filled with 150 µl of HEPES buffered solution (Lonza).
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bioRxiv - Cell Biology 2024Quote: For CRISPR editing RNPs containing 6μl of sgRNA (30 pmol/μl) and 1μl Cas9 (20 pmol/μl) were assembled in 13μl Nucleofector TM solution (LONZA) at room temperature for 30mins ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 × 106 of EGFP-PGCs were resuspended in a total volume of 100 μl Nucleofector Solution V (Lonza, Switzerland) premixed with 10 μg of Cas9 plasmid and the same amount of phiC31 integrase ...
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bioRxiv - Biochemistry 2020Quote: ... The complexes were separated from unbound species by electrophoresis on a 0.7% nondenaturing gel using a SEAKEM GTG agarose solution (Lonza) made with 1× TBE buffer ...
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bioRxiv - Cell Biology 2021Quote: Vero cells were transfected with 2 µg plasmid DNA in the Amaxa Nucleofector solution R (Lonza Bioscience, Rockville, MD), following the manufacturer’s instructions with program V-01 ...
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bioRxiv - Genomics 2022Quote: ... Two million cells were resuspended in 100 μl of P3 Primary Cell Nucle-ofector™ solution (V4XP-3024, Lonza). The RNP complex and Alt-R® Cas9 Electroporation Enhancer (1075916 ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 000 to 100 000 cells per reaction was then resuspended in 20uL of P3 solution (Lonza, V4XP-3032) and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... cells were nucleofected using Nucleofector SE solution and reagent into Nucleocuvette following manufacturer’s instructions using 4D-Nucleofector System (Lonza). The cell number and nucleic acid amounts are indicated in each figure legends ...
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bioRxiv - Biochemistry 2021Quote: ... For each nucleofection 3 x 106 cells were resuspended in 80 μl of mouse ES cell nucleofection solution (Lonza). Equimolar (0.32 nmol ...
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bioRxiv - Immunology 2022Quote: ... The solution was centrifuged at 400G for 5 minutes at 4°C and the cell pellet was resuspended in 1mL of Hank’s Balanced Salt Solution (HBSS; Lonza) containing 1% BSA (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Five hundred thousand cells were pelleted and resuspended in RNP solution and 20ml of primary cell P3 buffer (Lonza), and were transferred to an electroporation curvette (Lonza) ...
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bioRxiv - Biophysics 2021Quote: ... and resuspended at a density of ∼1.5x104 cells/μL in SF Cell Line 4D-Nucleofector Solution SF with supplement according to manufacturer instructions (Lonza). 2 μg of each Pcdh expression construct were transfected into 20 μL of the K562 cell suspension by electroporation using an Amaxa 4D- Nucleofector (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: ... washed twice with PBS and 0.25×106 cells per condition resuspended in 20μL supplemented cell line solution (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were then transferred to the RNP solution ...
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bioRxiv - Cancer Biology 2023Quote: ... 3uL of the total Cas9-RNP complex was added to 200,000 K562 cells resuspended in 18uL SF cell solution (Lonza) and subjected to nucleofection with the Lonza 4D nucleofectorsystem using manufactured specified conditions for K562 cells ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 T-cells were pelleted and re-suspended in 100 μL nucleofection solution (Lonza, Walkersville, MD, USA). T-cell suspensions were mixed with 5 μl RNP complex and cell/RNP mix transferred to Nucleofector cuvette ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use in functional assays.
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 20 µL of SF Cell Line Nucleofector™ Solution with the supplement added (Lonza V4XC-2032). The cells and the RNP complex were carefully mixed and transferred to a well of the 16-well Nucleocuvette™ ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL of cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Genomics 2023Quote: ... Cells were grown at 37°C and 5% CO2 and passed with Hepes buffered saline solution (Lonza, CC-5024) and 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Both B-ALL and CAR-T cells were prepared for transplantation by resuspending in 200 μL Hank’s balanced salt solution (Lonza) and loaded in 27.5-gauge syringes (Becton Dickinson) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were mixed with complete p3 electroporation solution (from a Lonza 4D nucleofection kit; Lonza Group AG, Basel, Switzerland) followed by addition of the RNP complex and 500 pmoles of donor ssODNA (Alt-R HDR ...
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bioRxiv - Microbiology 2022Quote: ... 20-25 μl of pure late schizonts were mixed with 90 μl of P3 solution in a cuvette (Lonza) containing 20 μg of repair template and 10 μg each of two guide vectors (total 40 µg) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted at 1000 rpm for 5 min and resuspended in 100 μL nucleofector solution (Lonza, #VPB-1002) before adding 2.5 μg of plasmid ...
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bioRxiv - Physiology 2023Quote: Purified HA was mixed with sucrose solution (final concentration of 333 mM) and loaded to a 0.4% SeaKem Gold agarose gel (Lonza). HA-Ladders (Hyalose ...
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bioRxiv - Immunology 2024Quote: ... and resuspended at 7.5E4 cells/µL in freshly supplemented P3 Primary Cell Nucleofector Solution (Lonza, catalog no. V4SP-3096). 20 µL of cell were mixed with 7 µL RNP ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use.
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bioRxiv - Biophysics 2024Quote: Gel slides for bacterial spatial fixation were prepared as follows: A solution of 1.5% (w/v) low melting agarose (Lonza) in PBS was heated for approximately 20 seconds to allow full melting of the agarose ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellet was resuspended in 20 µL of pre-warmed P3 primary cell solution (Lonza Kit, V4XP-3032), 2.5 µL RNP complex ...
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bioRxiv - Developmental Biology 2024Quote: ... larvae were anesthetized using 0.0053% tricaine in a PTU/E3 solution and then mounted on a glass microscopy slide using 2.5% low-melt agarose (SeaPlaque, Lonza) in a PTU/E3 solution containing 0.016% tricaine ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 clonal Kp03 landing pad cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended and transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution in a 25 µL electroporation cuvette (Lonza). Electroporation of GCaMP mutants was performed according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed with 1X phosphate buffered saline (PBS) and resuspended in 20 µL of solution SF or SE (Lonza). Cell suspensions were combined with RNP complex(es) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Genomics 2020Quote: ... 3 μL RNP complex solution was mixed with the cells and the cells were nucleofected using program DJ-110 on a 4D-Nucleofector (Lonza) with 16-well strips ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution electroporation cuvettes (Lonza). Electroporation was performed according to the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... between 1 x 106 and 1 x 107 cells were resuspended in 18 μl of P3 primary cells solution (Lonza), 2 μl of the restricted cassette (2-4 μg total ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μg of linearized plasmid containing wild-type or Ku70 3A cDNA was transfected using Amaxa nucleofector solution V (Lonza) and program T-020 ...