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Citations for Lonza :
501 - 530 of 530 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... An amount of 106 cells were resuspended in 100 µl of SE Cell Line Solution (Cat. No. V4XC-2024, Lonza, Basel, Switzerland) containing 3.5 µg of the respective plasmid and electroprated with pulse DG113 ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
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bioRxiv - Immunology 2024Quote: ... sgRNAs targeting Mavs and NLSx2-Cas9 protein were mixed with FLAMs in P2 primary cell nucleofection solution (Lonza, catalog no. V4XP-2024). Nucleofection was carried out using the 4D-Nucleofector® Core and X Unit (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
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bioRxiv - Microbiology 2024Quote: One million K562 cells were electroporated with 3 μg of DENV2-luciferase replicon [38] (provided by Jan Carette, Stanford University) in 100 μL SF Cell Line Nucleofector solution (Lonza V4XC-2012) using pulse code FF-120 (Amaxa 4D Nucleofector ...
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bioRxiv - Immunology 2024Quote: ... One million NK cells were then resuspended in 20 µl of 4D Nucleofector Master Mix (82% P3 solution + 18% Supplement 1; Lonza, V4XP-3032) and mixed with the Cas9 RNP complex for electroporation using the CM137 program ...
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bioRxiv - Genomics 2024Quote: ... Approximately 12 million cells per replicate were spun down and resuspended in nucleofection solution from the Lonza SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2012) and nucleofected in 12 reactions with 5 μg pLJK07 or pLJK08 per 1 million cells according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 OVCAR8 landing pad cells were resuspended in 20 µl of nucleofector solution combined with supplement at 4.5:1 from the Amaxa SE Cell Line 4D-Nucleofector X Kit S (Lonza, Cat #V4XC-1032), before addition of 5 µl RNP complex and 1 µl of Electroporation enhancer ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection was performed by electroporation using an Amaxa Nucleofector II combined with Human Stem Cell Nucleofector Solution 2 (Lonza, ref #: VPH-5022) and programme B-16 ...
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bioRxiv - Cell Biology 2021Quote: ... The solution was transferred into a certified cuvette and cell electroporation was conducted using Nucleofector® program X-013 (Amaxa® Nucleofector®, Lonza). Electroporated cells were immediately resuspended in pre-warmed phenol-free Leibovitz’s-L-15 medium (Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...
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bioRxiv - Genomics 2021Quote: ... Growing CH12F3 cells were transfected with 1.5 μg pX330-Cas9 or pX330-Cas9-mCherry expression vector per million by 4D-nucleofector X (Lonza, solution M1, procedure DN100) and seeding at 0.5 million cells/mL in fresh medium with 1μg/mL anti-CD40 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets of 1E6 cells were resuspended with 20ul supplemented nucleofector solution of the P3 Primary Cell 4D-nucleofector® X Kit S (Lonza, V4XP-3032) and 2ug plasmid (1ug/ul) ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Cell Biology 2023Quote: ... Next 1 × 106 cells resuspended in 93 μL of P3 Primary Cell Nucleofector Solution (P3 Primary Cell 4D-Nucleofector X Kit, Lonza, Catalog #: V4XP-3024) were mixed with 7 μL of RNP and 12 μg of single-stranded oligodeoxynucleotide (ssODN ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5x105 iPSCs were resuspended in 20μL of P3 primary cell nucleofector solution plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) along with the RNP complex and nucleofected using Lonza 4D Nucleofector (program CA-137) ...
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bioRxiv - Cell Biology 2024Quote: ... This mixture was incubated for 10 min-at room temperature and further diluted with 20 μL of P3 Primary Cell solution (Lonza, Walkersville, MD, USA). For the nucleofection process ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 x 106 REH cells were transfected with 200 pmol of each siRNA pool (siCtrl, siH1-0_1, siH1-0_2) in the 100 µl nucleocuvette format using the 4D Nucleofector system (Lonza, SF solution, program DS-150).
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bioRxiv - Bioengineering 2021Quote: ... We then electroporated 4.0×105 of wildtype or the stably integrated mCherry K562 cell lines in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with pJT396 in combination with an equimolar amount of either pUC19 or a Cp36 expression vector ...
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bioRxiv - Bioengineering 2021Quote: ... were subsequently integrated into the AAVS1 locus of cells expressing rTetR-VP48 using TALEN-mediated homology-directed repair as follows: 1.0×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T0-16) with 1000 ng of reporter and 500 ng of each TALEN-L (Addgene #35431 ...
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bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...
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bioRxiv - Genetics 2020Quote: ... Reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate a donor construct into the AAVS1 locus as follows: 1.2×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T0-16) with 1000 ng of reporter donor plasmid (Table S2) ...
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bioRxiv - Cell Biology 2023Quote: ... to aid complete removal of PBS before resuspension in P3 Nucleofection Solution (room temperature; P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza; cat. no., V4XP-3032). A total of 20 μl solution was used to resuspend 80,000 cells ...
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bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
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bioRxiv - Cell Biology 2022Quote: A control plasmid (GFP) or GFP-CHMP4B were overexpressed in WI-38 cells by 4D-Nucleofector (Lonza; SE cell line solution; program EO-114) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Reporter cell lines were generated by TALEN-mediated homology-directed repair via integration of a donor construct into the AAVS1 locus of cells as follows: 1.2×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T-16) or 8×104 HEK293T cells were transfected using Lipofectamine LTX (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Mm.Cas9.NOS2.1.AA) and 1 µM HiFi Cas9 enzyme (IDT, catalog no. 1081060) in 100 µL Nucleofector Solution V plus supplement (Lonza, catalog no. VCA-1003), using the preprogrammed electroporation protocol B-032 on Nucleofector II/2b ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 K562 cells harboring a monoclonal Kp03 landing pad and multiclonal rTetR-VP48 expression construct were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1000 ng of reporter donor plasmid (pMMH56,59,59) ...