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Citations for Lonza :
451 - 500 of 530 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The cells were first electroporated with a PiggyBac-configured plasmid containing the Dox-inducible NbALFA-ABEL degrader cassette and a plasmid encoding the PiggyBac transposase at a 3:1 mass ratio via nucleofection (solution E with program CM138) according to manufacturer’s instructions (Lonza Inc.), followed by puromycin selection (5μg/ml ...
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bioRxiv - Bioengineering 2024Quote: 3·106 BRPKp110 cells were resuspended in 20 µl primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X kit S (32 RCT, V4XP-4032; Lonza). Cells were mixed and incubated with 15 µl RNP at room temperature for 2 minutes ...
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bioRxiv - Genetics 2024Quote: 61 pmol Recombinant Alt-RspCas9 protein (IDT) in 10 µL of Human Keratinocyte Nucleofector™ Kit solution (Lonza, VPD-1002) for 10 min and immediately used for nucleofection of 8×105 primary keratinocytes with the Amaxa nucleofection apparatus (Lonza ...
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bioRxiv - Bioengineering 2024Quote: ... and resuspended at a concentration of 2.5 x 105 cells in 20 μl of pre-mixed P3 Primary Cell 4D-Nucleofector solution (P3 Primary Cell 4D-Nucleofector X Kit S, Cat: V4XP-3032; Lonza). Cells were combined with the RNP/HDR plasmid solution and transferred into individual wells of a 16-well Nucelocuvette Strip (Lonza) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were combined with the RNP/HDR plasmid solution and transferred into individual wells of a 16-well Nucelocuvette Strip (Lonza). Nucleofection was performed using 4D-Nucleofector Core (Cat ...
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in 20 µL of supplemented SE Nucleofector Solution as per the manufacturer’s guidelines for HeLa and HCT116 cells (Lonza Bioscience). The entire RNP complex solution and 1 µL 100 µM Alt-R Cas9 electroporation enhancer (IDT ...
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bioRxiv - Cell Biology 2024Quote: ... 0.3×106 live cells were resuspended with the RNP complex and 20 µL of P3 Primary Cell Nucleofector Solution (Lonza), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 106 cells were centrifuged at 90 x g for 10 minutes and then resuspended in 100 μl nucleofector solution V (Lonza). The cells were combined with 2000 ng plasmid DNA and placed in a specially designed cuvette ...
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bioRxiv - Microbiology 2023Quote: Embryonic monkey kidney cells (MA104) were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 4.5 g/L glucose (Lonza 12-640F or Corning 15-107-CV), 1% penicillin-streptomycin [Corning]) ...
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bioRxiv - Bioengineering 2021Quote: ... we nucleofected HEK293FT-RfxCas13d cells with 225 μmol of synthetic crRNAs in 20μl reaction of SF Cell Line Nucleofector Solution (Lonza V4XC-2032) using the Lonza Nucleofector 4D (program CM-130) ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cell suspension with trypsin-EDTA (Pan-Biotech, Germany) for 3 min followed by trypsin neutralizing solution (Lonza, Germany). Single cell suspensions of secondary mammospheres were obtained as described for day 7-first generation mammospheres.
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bioRxiv - Neuroscience 2022Quote: We prepared thin agar pad for the samples where 8 µL of semi-cooled 1.5% agarose solution (add low melting temperature NuSieve GTG Agarose (Lonza, Cat# 50081) to 2% galactose media (0.77 g of CSM-URA (Sunrise # 1004-010) ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended with 10µL of primary cell nucleofection solution (P3 primary Cell 4D-Nucleofector X kit S (Lonza, # V4XP-3032), and mixed with RNP complexes ...
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bioRxiv - Microbiology 2022Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and to a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC5002); this was repeated to maximize cell collection ...
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bioRxiv - Developmental Biology 2020Quote: ... or a PMO Control at 100 µM in 100 µL solution from the P3 Primary Cell 4D-Nucleofector® X Kit L (V4XP-3024, Lonza) using the CB150 program on the 4D-Nucleofector™ System (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
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bioRxiv - Genomics 2023Quote: ... and 120 pmol of in vitro synthesized gRNA were electroporated into 250,000 MCF7 cells with the P3 primary nucleofection solution (Lonza, V4XP-3024), using the DN-100 Lonza 4D-Nucleofector program ...
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bioRxiv - Genetics 2023Quote: All iPSC lines were passaged as clumps or single cells upon reaching 80-85% confluency using Versene (0.02% EDTA solution, Lonza #17-711E) or StemPro Accutase (Gibco #A1110501) ...
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bioRxiv - Molecular Biology 2022Quote: ... collected by centrifugation at 500xg for 5 min and resuspended in SF solution (SF cell line 4D-Nucleofector™ X Kit, Lonza). After addition of the RNP ...
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bioRxiv - Biophysics 2023Quote: ... 1.5mL for a T75 flask for 5 minutes at 37° C and resuspended in 80 μL of SF cell line solution (Lonza; Basel) per flask ...
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bioRxiv - Genomics 2022Quote: ... We used 1.0 × 106 HCT116 or U2OS cells to transfect the base editor plasmids (2600 ng) using SE solution and 4D-nucleofector (Lonza, Switzerland) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Sections attached to slides were fixed with cold acetone/ethanol (1:1) solution and washed in PBS buffer (Lonza, Morristown, NJ). Primary antibodies and isotype controls (Supplementary Table 9 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 µL of cell suspension (200,000 cells) was mixed with the RNP-containing solution and electroporated using the 4D-Nucleofector X Unit (Lonza, Basel, Switzerland) with the CM138 nucleofection program ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 4 million cells (48 h knockdown ...
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bioRxiv - Immunology 2024Quote: ... The DPP4 KO and NTC groups were washed with 1X PBS and resuspended with P3 primary nucleofection solution (Lonza, cat. V4XP3012) containing ribonucleoprotein (RNP ...
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bioRxiv - Biochemistry 2024Quote: ... 5.0 × 106 OSCs were mixed with 200 pmol of siRNAs in 20 μL of Solution SF of the Cell Line Nucleofector Kit SF (Lonza Bioscience) and electroporation was performed using a Nucleofector 96-well Shuttle device (Lonza Bioscience) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza) ...
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bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
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bioRxiv - Microbiology 2022Quote: ... Cells that were harvested at 48 hours were first washed by dispensing and aspirating 37°C HEPES buffered saline solution (Lonza, CC-5022), and then trypsinized with 0.025% Trypsin/EDTA (Lonza ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Biochrom AG, Berlin, Germany) and 1% (v/v) antibiotic–antimycotic solution (AAS, Lonza, Basel, Switzerland) was used for culturing human dermal fibroblasts (HDF ...
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bioRxiv - Immunology 2020Quote: ... 50 pmol sgRNA and 20 pmol Cas9 were combined and diluted to 20 µL with nucleofection solution P1 or P3 (with supplement added, Lonza, Basel, Switzerland). The mixture was incubated at 25 °C for 10 min or up to 2 h and immediately used to electroporate moDCs ...
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bioRxiv - Genomics 2021Quote: ... mESCs in 6-cm dishes were transfected with 7.2 μg pX330-Cas9 plus 1.8 μg GFP expression vector by 4D-nucleofector X (Lonza, solution Cytomix, program GC104), then harvested for genomic DNA 3 days after transfection.
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bioRxiv - Neuroscience 2020Quote: ... dissociated ganglia were pelleted at 100 x g for 5 min and resuspended in 100 µl ‘Nucleofector solution’ (Rat Neuron Nucleofector kit; Lonza, Alpharetta, GA). 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG ...
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bioRxiv - Molecular Biology 2022Quote: ... 480 pmol L2 gRNA targeting BFP and 500 pmol BFP to GFP ssODN template using CM-189 and SF solution (Lonza, 4D electroporator) per replicate ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Biochemistry 2024Quote: 1 µL of 63 µM Alt-R™ S.p.Cas9 V3 (IDT: 10007807) was diluted with 18 µL of SE Cell Line Nucleofector® Solution (Lonza V4XC-1032), followed by the addition of 1.8 µL of 100 µM sgRNA (3:1 sgRNA:Cas9) ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1.0*106 iPSCs were gathered as single cells as described under ‘cell lines and cell culture’ and resuspended in 100 μL NucleofectorTM Solution V (Lonza, Basel, Switzerland) and added to the reaction mixture ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were resuspended in 20 µl nucleofection buffer (16.4 µl Nucleofector® Solution + 3.6 µl Supplement) provided in P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032). After the addition of 3 µl RNP and 0.5 µl of Alt-R Cas9 Electroporation Enhancer (IDT ...
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bioRxiv - Cell Biology 2024Quote: ... and sgRNAs (see Key Resources Table) were delivered by nucleofection to a single cell suspension of 105 iBCs in Primary Cell P3 solution (Lonza, #V4XP-3032). Cells were re-cultured and the resulting clonally-derived spheroids were isolated ...
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bioRxiv - Cell Biology 2023Quote: ... The linearized construct and the purified schizonts were mixed with Nucleofector solution from an Amaxa human T cell Nucleofector Kit and electroporated using the Amaxa Nucleofector II device (Lonza, Köln, Germany). Directly after transfection 50 µl RPMI-1640 complete was added to the transfection reaction followed by intravenous injection into one SWISS mouse ...
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bioRxiv - Systems Biology 2024Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and placed in a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC-5002); this was repeated to maximize cell collection ...
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bioRxiv - Microbiology 2023Quote: Colons from infected mice were open longitudinally and gently washed with ice-cold 1X PBS before being placed in Hanks’ balanced salt solution (HBSS) with phenol red and without calcium and magnesium (Lonza, Basel, Switzerland) on ice ...
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bioRxiv - Genomics 2023Quote: ... were washed twice with PBS and resuspended in a solution containing a 4.5:5 mixture of nucleofection buffer (Buffer SE, Lonza Lot #: S-09279) and supplement (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were fabricated using a solution consisting of lyophilized GelMA (5 wt%) dissolved at 37°C in phosphate buffered saline (PBS; Lonza 17-516F) and combined with 0.1% w/v lithium acylphosphinate (LAP ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 105 single cells were resuspended with Lonza P3 Primary Cell Nucleofector® Solution and mixed with the pre-formed RNP complexes and transferred to a Nucleocuvette™ (Lonza). Nucleofection was performed using program EO-115 on the 4D Nucleofector™ X unit (Lonza) ...
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bioRxiv - Genetics 2024Quote: ... erythroleukemia K562 cells were washed in 50 mL PBS at RT and approximately 1×106 cells were resuspended in SF solution (Lonza V4XC-2032) and electroporated in presence of pre-assembled Cas9:gRNA complexes plus 12 µg ssDNA (100 µL format ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs pre-stimulated for two days were washed in 50 mL PBS at 37° C and resuspended in P3 nucleofector solution at RT (Lonza, V4XP-3032). Approximately 2.5×105 CD34+ HSPCs were mixed with gRNA:Cas9 complexes and 3 µg ssDNA donor template (20 μL ...