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Citations for Lonza :
2551 - 2572 of 2572 citations for PGFM ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... were sorted into B cell media (IMDM medium, GIBCO; 10% heat-inactivated low IgG FBS, Life Technologies; 5 ml GlutaMAX, Life Technologies; 1 ml MycoZap plus PR, Lonza). Immediately following the sort ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza, Walkersville, MD, USA). The generation number of ECs is maintained between 30-40 generations ...
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bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
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bioRxiv - Microbiology 2021Quote: ... were isolated by Ficoll density gradient centrifugation as described previously [22] and seeded at a density of 1×106 cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Systems Biology 2022Quote: ... The PGK reporter cell line was generated by electroporation of K562 cells with 0.5 ug each of plasmids encoding the AAVS1 TALENs and 1 ug of donor reporter plasmid using program T-016 on the Nucleofector 2b (Lonza, AAB-1001). Cells were treated with 0.5 ug/mL puromycin for one week to enrich for successful integrants ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Genetics 2024Quote: ... erythroleukemia K562 cells were washed in 50 mL PBS at RT and approximately 1×106 cells were resuspended in SF solution (Lonza V4XC-2032) and electroporated in presence of pre-assembled Cas9:gRNA complexes plus 12 µg ssDNA (100 µL format ...
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bioRxiv - Cell Biology 2020Quote: ... cells were serum starved for at least 13 h in 1% FBS (Atlanta Bio selected, Cat #S11110) or without FBS using endothelial cells growth basal medium (Lonza cat#cc-3121) instead of MCDB-131-complete media ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was then placed in a customized 35mm petri dish with sylgard-carved imaging chamber and covered with 1% low-melting agarose (Lonza #50101, Rockland, ME) for extra immobilization ...
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bioRxiv - Microbiology 2021Quote: ... NHBE cells were seeded in 24-well plates at 1×105 cells in a final volume of 0.5 ml BEGM™ Bronchial Epithelial Cell Growth Medium (BulletKit™; Lonza, Basel, SW). Cultures were incubated overnight 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... Conditioned media (containing EVs) was removed and the cells washed x 2 with PBS before trypsinisation using 1 × Trypsin-EDTA (Lonza, UK cat: T3924). Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5x105 iPSCs were resuspended in 20μL of P3 primary cell nucleofector solution plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) along with the RNP complex and nucleofected using Lonza 4D Nucleofector (program CA-137) ...
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation and washed once with 50:50 TE and resuspended in 50ul SybrGreen (1:50 dilution with 50:50 TE; Lonza, CAT#12001-798, 10,000X) and incubated overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... EBs were transferred to the final format of choice and left undisturbed for 1 week in factory media (X-VIVOTM-15 (Lonza, catalog no. BE02-060F) 1 % GlutaMax (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... 2 million of CAR-T cells were electroporated with 1 μg Cas9 mRNA in 16-well Nucleocuvette™ Strips using EO-115 program (Lonza, per manufacturer’s instructions). Cells were expanded in human T cell media for another 3 days before analysis or transfer into mice.
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bioRxiv - Immunology 2023Quote: ... 2 million of CAR-T cells were electroporated with 1 μg Cas9 mRNA in 16-well Nucleocuvette™ Strips using EO-115 program (Lonza, per manufacturer’s instructions). Cells were expanded for another 2 days before transfer into NSG mice.
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bioRxiv - Cancer Biology 2023Quote: ... The trachea was then exposed and a 21G needle was inserted to either 1) inflate the airway with 2cc of 1.5% low melt agarose (Lonza SeaPlaque™; Tm 65°C) for immediate fixation in cold 4% paraformaldehyde (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Mm.Cas9.NOS2.1.AA) and 1 µM HiFi Cas9 enzyme (IDT, catalog no. 1081060) in 100 µL Nucleofector Solution V plus supplement (Lonza, catalog no. VCA-1003), using the preprogrammed electroporation protocol B-032 on Nucleofector II/2b ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 10,000 units/l (penicillin) and 1 mg/l (streptomycin) and 2 mM L-glutamine (BioWhitaker, Lonza, 200 mM, cat. No. 17-605E), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were then mechanically triturated and washed in a complete neurobasal A medium (NBA, Gibco® Invitrogen™ supplemented with 2% B27, 2mM L-glutamine and 1% antibiotics: penicillin + streptomycin, Lonza™ BioWhittaker™), before being plated in 35- mm petri dishes ...
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bioRxiv - Molecular Biology 2019Quote: ... 150,000 cells were resuspended in 20 µL of P3 Primary Cell Nucleofector Solution with Supplement 1 and mixed with the gRNA/rCas9 solutions before electroporation (4D-Nucleofector System, Lonza, Walkersville, MD; program DC-100). For experiments involving the Neon Transfection System (Thermo Fisher Scientific) ...