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Citations for Lonza :
2101 - 2150 of 2572 citations for PGFM ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Genomics 2019Quote: ... The products were size selected on a 3% NuSieve 3:1 Agarose gel (Lonza), purified using NucleoSpin Gel and PCR clean-up columns ...
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bioRxiv - Bioengineering 2020Quote: ... Fluidic lines were coated with 1% gelatin which was replaced by EGM-2 (Lonza) for up to 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of each pX330-gRNA plasmid plus 0.1 μg of pmaxGFP plasmid (Lonza) were transiently transfected into exponentially growing cells using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... CD-1 mouse embryonic fibroblasts (MEFs) were obtained from Lonza (Cat#M-FB-481) and inactivated with 10 μg ml−1 mitomycin C (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... human THP-1 cells were maintained in Roswell Park Memorial Institute medium (RPMI; Lonza). All medium were supplemented with 2-5 or 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2020Quote: ... at 37°C for 1 hour and the released cells resuspended in DMEM (Lonza), supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... 1 × 1010 freshly collected conidia were incubated shaking in 50 mL RPMI 1640 (Lonza) for 5 hours (h ...
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bioRxiv - Molecular Biology 2020Quote: hTERT-immortalized Retinal Pigment Epithelial cells (RPE-1) were cultured in DMEM-F12 (Lonza) containing 10% FBS (Bodinco) ...
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM sodium pyruvate (BioWhittaker® Medium EMEM Cell Culture Media, Lonza, Maryland, USA), and 10% fetal calf serum ...
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bioRxiv - Cell Biology 2021Quote: Human Umbilical Vein Endothelial Cells (HUVEC) and THP-1 cells were purchased from Lonza Walkersville Inc ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 10% Fetal Bovine Serum (FB12999102, FisherScientific) and 1% Penicillin-Streptomycin (Lonza,17602E). To extract sufficient poly-A RNA ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Neuroscience 2024Quote: ... The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza). 2.5 × 10 5 phNPCs cells were used per electroporation reaction in one cuvette of the 16-well Nucleocuvette Stripe (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 × 106 iPSCs were resuspended in 100 μl of P3 buffer (V4XP-3024, Lonza) containing 20 μg Alt-R® S.p ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Microbiology 2023Quote: ... with a final concentration of 2% formaldehyde and 2% NuSieve 3:1 agarose (Lonza). 5 µg RNA was denatured for 10 min at 70 °C with 1 X MOPS buffer (20 mM MOPS ...
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bioRxiv - Neuroscience 2023Quote: ... Individual larvae were mounted in a droplet of 1% low melting agarose (50100, Lonza) in the middle of a 60 mm x 15 mm Petri dish and immersed in system water after the agar solidified ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S, Lonza). For both cell types ...
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bioRxiv - Developmental Biology 2023Quote: ... and were separated by electrophoresis in 1-1.2% (w/v) agarose gel (Lonza 50004) supplemented with 0.03μl/ml DNA staining dye (Serva ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S, Lonza). Human SH-SY5Y neuroblastoma cells (ATCC ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained spheroplasts were next embedded into 1 % low melting agarose (InCert Agarose 50123, Lonza) plugs and incubated overnight at 55 °C in a digestion buffer with 1 mg/mL of proteinase K (Euromedex EU0090) ...
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bioRxiv - Biophysics 2021Quote: ... for all imaging experiments involving exogenous expression we used the Lonza Amaxa II Nucleofector System with Cell Line Nucleofector Kit V reagent (Lonza VCA-1003). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs were transfected into 3T3-F442A preadipocytes or OP9 preadipocytes via electroporation using the Amaxa Cell Line Nucleofector Kit V (Lonza, Cologne, Germany). Afterwards cells were grown two days in DMEM ...
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bioRxiv - Developmental Biology 2021Quote: Human isogenic ESCs containing different lengths of CAG repeats in the HTT Exon1 locus (HD-RUES2 6) were nucleofected using the Cell Line Nucelofector II (Kit L from Lonza, Walkersville, MD) by applying the B-016 program ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Electroporation of K562 cells was performed using a SF Cell Line 4D-Nucleofector kit and 4D-X Nucleofector using pulse code FF-120 (Lonza, Basel, Switzerland), per the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and CD31+Rspo3+ cells were centrifuged at 500 g for 5 min and resuspended in 500 μl endothelial cell media: EGM-2 MV Bullet Kit (Lonza, cc-3202) supplemented with 50 ng/mL VEGF-C (R&D ...
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bioRxiv - Molecular Biology 2020Quote: ... 4×106 cells were electroporated with 10 μg DNA of pBluescript II SK(+) carrying a human U6 promoter and EGFP-sgRNA using Nucleofector® V Kit (Lonza, Switzerland) as described above.
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The siRNA knockdown was performed by electroporation using the Amaxa nucleofector I in combination with the Amaxa Cell Line Nucleofector Kit V (Lonza, VCA-1003). SiRNA constructs with the siRNA cloned in the pLKO.1-Puro vector were obtained from the MISSION shRNA library (Sigma product SHGLY) ...
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bioRxiv - Microbiology 2020Quote: ... using the AMAXA Nucleofector 4D system (U-033 setting) and the P3 primary cell 4D-nucleofector X kit with the 16-well nucleocuvette strip (Lonza, V4XP-3032). Clones were obtained by FACS using the FACS Aria II sorter at the Stanford Shared FACS Facility for the brightest red parasites and single cloning the TdTomato+ enriched population by limiting dilution into 96-well plates.
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bioRxiv - Bioengineering 2019Quote: Nucleofection of the HDR template and CRISPR-Cas9 vectors was performed with SF Cell Line 4D-Nucleofection X Kit L (Lonza, V4XC-2024). Before nucleofection hybridoma cells were assessed for viability and centrifuged (90g ...
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bioRxiv - Neuroscience 2019Quote: ... were provided under a MTA and maintained in Clonetics® endothelial cell growth medium-2 MV Bullet Kit (Cat# CC-3162 Lonza, UK) containing the endothelial basal medium ...
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bioRxiv - Neuroscience 2019Quote: ... C57BL/6J: L315 control construct or L315 Nrxn triple knockdown construct) just before plating using AMAXA Nucleofector kit (Lonza, Cat #VPG-1001) and plated at 2500/cm2 on micro islands of mouse (CD-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 µg of PX459 gRNA vector (5’-GACTCCAGTCTTTCTAGAAGA-3’) were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-100) using program A-30 in the XRep ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 μg of the donor ssDNA and 3 μg of a puromycin resistance plasmid (pCAG-puroR) using the Mouse ES Cell Nucleofector™ Kit (Lonza, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines identities were validated by STR profiling and tested negative for mycoplasma with MycoAlert Mycoplasma Detection Kit (Lonza, Cat#LT07-418) prior to experimental use.
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells (grown in 6-well dishes) were subjected to nucleofection with Amaxa® Cell Line Nucleofector® kit V (VCA-1003, Lonza), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 bulk or stable populations overexpressing Nluc-CD63 or Nluc-CD9 constructs were obtained using an electroporation-based transfection protocol optimized for this cell line (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... subjected to red blood cell lysis with ACK lysis buffer and electroporated with P3 Primary Cell 4D-NucleaofactorTM X -kit (V4XP-3032, Lonza, Basel, Switzerland), as previously described (Freund et al. ...
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bioRxiv - Cell Biology 2021Quote: ... OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences) at each time point ...
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bioRxiv - Cell Biology 2021Quote: ... We transfected the KCNQ1 constructs and the empty vector into our established mouse pancreatic β cells (referred to as SJ β cells (Jia et al., 2015)) using AmaxaTM Cell Line NucleofectorTM Kit V and AmaxaTM NucleofectorTM 2b device (Lonza, Switzerland). The glucose-sensitive mouse β-cell line SJ-β was cultured and an insulin-secretion assay performed as reported earlier (Jia et al. ...
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bioRxiv - Systems Biology 2021Quote: ... freshly isolated neurons (5 million cells per nucleofection reaction) were transfected using the 4D-Nucleofector™ X Unit and the P3 Primary Cell 4D Nucleofector X kit (Lonza, Switzerland), as indicated ...
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bioRxiv - Cell Biology 2020Quote: ... These plasmids were transfected into NHDFs and 7SPs by electroporation using the Amaxa nucleofector kit and nucleofector 2b (Lonza, Walkersvill, MD, USA). Two days later ...
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bioRxiv - Cancer Biology 2021Quote: ... All human cell lines were authenticated by Short Tandem Repeat analysis and tested for Mycoplasma contamination with a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Genomics 2022Quote: ... 5 million cells were transfected with 10ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit T (Lonza), program X-001 ...
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bioRxiv - Molecular Biology 2022Quote: ... Electroporation of 5×106 HL-60 cells was performed with AIO-GFP HAX1 LCRC1 and AIO-GFP HAX1 LCRC2 using CLB-Transfection™ Kit (Lonza, Austria) and CLB-Transfection™ System (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines were cultures for no more than 30 passages and routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza, Slough, UK).
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...