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Citations for Lonza :
1251 - 1300 of 1369 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Genomics 2022Quote: ... 5 million cells were transfected with 10ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit T (Lonza), program X-001 ...
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bioRxiv - Biochemistry 2022Quote: ... For dual sgRNA gene editing 100 pmols of Cas9 RNP with cut site A sgRNA and 100 pmols of Cas9 RNP with cut site B sgRNA were nucleofected (Lonza 4D nucleofector) with CA137 pulse code ...
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bioRxiv - Immunology 2022Quote: 1 × 105 HLFs were mixed with 2 μg of 100 μg/ml of the above expression plasmids in 100 μl PBS (GE Lifesciences, Marlborough, MA) and were transfected by electroporation using a 4D-Nucleofector System (Lonza, Walkersville, MD) following the manufacturer’s protocol CZ-167 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were seeded onto 96-well plates at a density of 3×104 cells per well in 100 μL cell culture media that consisted of DMEM (Lonza, Basel, Switzerland), 1% penicillin–streptomycin (Lonza ...
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bioRxiv - Biophysics 2023Quote: ... The cells were maintained in a 100 mm cell culture dish using SmGM2 Smooth Muscle Cell Growth Medium BulletKit (SmGM2, CC-3182, Lonza, Basel, Switzerland) at 37 °C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... cells then were electroporated using electroporation code EH-100 and using the P3 Primary Cell 96-well Nucleofector Kit (Lonza, V4SP-3096). Knockout pools were maintained for an additional nine days prior to coculturing with H80 feeder cell line with IL-2 (Final conc 20 U/mL ...
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bioRxiv - Immunology 2023Quote: ... 80 µl of supernatant was removed and replenished with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1.0*106 iPSCs were gathered as single cells as described under ‘cell lines and cell culture’ and resuspended in 100 μL NucleofectorTM Solution V (Lonza, Basel, Switzerland) and added to the reaction mixture ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... 15 mL of molten normal melting point agarose was applied to GelBond® (Lonza, Switzerland) in a rectangular one-well plate (VWR) ...
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bioRxiv - Neuroscience 2020Quote: ... Red blood cells were lysed with 4 ml of ACK Lysis Buffer (Lonza, Walkersville, MD) per brain ...
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bioRxiv - Neuroscience 2020Quote: ... Mononuclear cells were collected by density centrifugation using lymphocyte separation media (1.077 g/mL, Lonza) as previously described 96 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.01 μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) (Lonza, Basel-Stadt, Switzerland) and 1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 0.1 μg/ml of interleukin (IL)-34 (IL-34) (Lonza, Basel-Stadt, Switzerland), 0.01 μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2020Quote: ... culture media supplemented with 0.1μg/ml of inter-leukin (IL)-34 (Lonza, Basel-Stadt, Switzerland) and 0.01μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.01μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) (Lonza, Basel-Stadt, Switzerland) was then added to these cells ...
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bioRxiv - Immunology 2022Quote: ... Purified B cells were then cultured at 0.5 ×106 cells/mL in RPMI 1640 (Lonza) containing penicillin (100 IU/ml) ...
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bioRxiv - Microbiology 2022Quote: ... at 200 µg/ml and incubated at 37°C overnight in DMEM (Gibco or Lonza) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 3 mL TheraPEAK™ ACK Lysing Buffer (1X) (Lonza, Basel, Switzerland). After a 2 minute incubation ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01 μg/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF) (Lonza, Basel-Stadt, Switzerland) and 1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.1 μg/mL of interleukin (IL)-34 (IL-34) (Lonza, Basel-Stadt, Switzerland), 0.01 μg/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary OCSC cultures were seeded at 5000 cells/ml in MEBM (Lonza, cat# CC-3151) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10% fetal bovine serum (GeminiBio, Sacramento, CA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2- betamercaptoethanol (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
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bioRxiv - Genomics 2020Quote: ... S2 cells were seeded at 2×106 per 6cm plate in 4 mL S2 medium (Lonza). One hour after plating ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed and resuspended at 2.5×106/ml in R5 medium (RPMI 1640, Lonza, Switzerland), 5% heat-inactivated FBS (Life Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... 5 × 107 hepatocytes were suspended in 30 mL of HCM™ (Lonza, Sales Ltd, Basel, Switzerland) and injected via the bile duct at a flow rate of 1 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... Exponentially growing Spodoptera frugiperda 9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium #BELN12-730Q) were infected with high-titer Baculovirus suspension ...
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bioRxiv - Evolutionary Biology 2019Quote: ... inactivated trypsin by adding 2 volumes of medium (500 ml DMEM (Lonza, Catalog no. BE12-733F), 55 ml FBS (PAN ...
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bioRxiv - Immunology 2020Quote: ... Antibodies which contained endotoxin levels above 3 EU/ml (Kinetic-QCL Kinetic Chromogenic LAL Assay, Lonza) were depleted of endotoxin with the ToxinEraser Endotoxin Removal kit (GenScript ...
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bioRxiv - Microbiology 2021Quote: ... Agarose-medium was made by autoclaving a 17.6 µg/ml SeaKem ME agarose (Lonza, Basel, Switzerland) dilution and heating it to 65°C ...
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bioRxiv - Bioengineering 2022Quote: ... 20 μL of T cells were resuspended in P3 buffer at 5 × 107 cells/mL (Lonza) and added to the electroporation mixture ...
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bioRxiv - Immunology 2022Quote: ... Absence of endotoxins (< 1EU/mL) was confirmed using the PyroGene™ Recombinant Factor C Assay (Lonza) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and the cell pellet resuspended in 2 ml of ACK lysing buffer (Lonza, Cat# 10-548E). After centrifugation ...
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bioRxiv - Immunology 2023Quote: ... with 0.05 mL blocking buffer (98% v/v FACS buffer (PBS + 2% v/v dFBS (Lonza)) and 2% v/v Fc receptor binding inhibitor (Thermo Fisher Scientific)) ...
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bioRxiv - Cancer Biology 2022Quote: Primary OCSC cultures were seeded at 5000 cells/ml in MEBM™ (Lonza, cat# CC-3151) supplemented with 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2021Quote: ... We then electroporated 4.0×105 of wildtype or the stably integrated mCherry K562 cell lines in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with pJT396 in combination with an equimolar amount of either pUC19 or a Cp36 expression vector ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Neuroscience 2019Quote: ... the dissociated neurons were centrifuged down to remove the supernatant and resuspended in 80 to 100 μl of Amaxa electroporation buffer for mouse neurons (Lonza Cologne GmbH; Cologne, Germany) with mixtures of siRNA (0.2 nmol per transfection ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 (see sequences of sgRNAs in Table S2) was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 K562 cells harboring a monoclonal Kp03 landing pad and multiclonal rTetR-VP48 expression construct were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1000 ng of reporter donor plasmid (pMMH56,59,59) ...
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bioRxiv - Genomics 2021Quote: ... PBMCs were then treated with 2 ml of erythrocytes lysis buffer (Lonza Walkersville inc. #120-02-070) for 1 min ...
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bioRxiv - Microbiology 2021Quote: Leukocyte transmigration assay was carried out by addition of 5×106 cells/mL hPBMC (#CC-2702 Lonza) to DENV2-infected HUVEC monolayers at 24 hpi following different treatments ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed with 5 mL of 1X-phosphate buffered saline without magnesium and calcium (PBS) (Lonza) and supplemented with fresh DMEM-HEPES supplemented with 10 mM sodium butyrate (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... EV pellets obtained from approximately 90 mL CM were re-suspended in 150-200 µL PBS (Lonza). Protein concentration was determined with the Bradford assay.
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bioRxiv - Molecular Biology 2020Quote: ... Days 16-24 media contained recombinant Oncostatin M (20ng/ml, Thermo) in HCM media lacking EGF (Lonza).
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bioRxiv - Bioengineering 2023Quote: ... the ground bones in 50 ml tubes were washed with phosphate-buffered saline (PBS, Lonza, Slough, UK) containing 2% penicillin/streptomycin (P/S ...
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bioRxiv - Cell Biology 2019Quote: ... Minced tissue were put into a 15-mL falcon tube filled with 3mL EBM-2MV (Lonza, Cologne, Germany) supplemented with 0.5% FBS ...