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Citations for Lonza :
1151 - 1200 of 1445 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 20 pmol Cas9 nuclease (sgRNA to Cas9 nuclease ratio used as 9:1) was performed with Amaxa 2D Nucleofector (Lonza, program B016). After recovery ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Biochrom AG, Berlin, Germany) and 1% (v/v) antibiotic–antimycotic solution (AAS, Lonza, Basel, Switzerland) was used for culturing human dermal fibroblasts (HDF ...
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bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
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bioRxiv - Microbiology 2021Quote: ... were isolated by Ficoll density gradient centrifugation as described previously [22] and seeded at a density of 1×106 cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of sgRNA and 5 µg of ssDNA donor template (Table S1) using Human Stem Cell NucleofectorTM kit 1 (Lonza, VVPH-5012) according to the manufactures protocol and cells replated ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with pcDNA3.1 empty vector or pcDNA3.1 containing the human coding sequence of the VGLL2-NCOA2 fusion using the AMAXA cell line Nucleofection kit V (Lonza #VCA-1003;) and were selected in growth media containing 1μg/ml concentration of G418 (ThermoFisher #10131035) ...
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bioRxiv - Molecular Biology 2023Quote: Induced pluripotent stem cells were transfected with a total of 5 μg target Cas9/gRNA plasmids with or without 1 μg repair templates using Human Stem Cell Nucleofector Kit (LONZA, Basel, Switzerland) and Nucleofector 2b device (LONZA ...
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bioRxiv - Genomics 2023Quote: ... 200,000 cells were nucleofected with 50 fmol transposon and 50 fmol transposase (Super piggyBac Transposase - SystemBio PB210PA-1) or with 300 ng pmaxGFP using the P2 Primary Cell 4D Nucleofector kit (Lonza V4SP-2096) and the Lonza 4D- Nucleofector with the DS-150 program ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Cancer Biology 2024Quote: ... after thaw for about 1 week and tested for mycoplasma regularly using the MycoAlertTM PLUS Mycoplasma Detection Kit (Lonza, CAT#: LT07-318). Cells were always used within 10 passages of authentication via STR analysis with CellCheck9 by IDEXX laboratories.
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bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 25 mL Amaxa Basic Parasite Nucleofector solution (Lonza) and added to 10 µg DNA dissolved in 10 µl H2O ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were stained with Hoechst33342 (6.7 μg/mL, Lonza) and propidium iodide (PI ...
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bioRxiv - Immunology 2020Quote: ... RBCs were lysed with 2 mL ACK lysis buffer (Lonza). Prior to surface staining ...
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bioRxiv - Microbiology 2021Quote: ... 0.05mg/ml gentamicin and 2% low melting point agarose (Lonza) and incubated at 37°C/5% CO2 for 72 hours ...
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bioRxiv - Physiology 2021Quote: ... In a volume of 0.1 mL Primary Cell Buffer (Lonza), 1.6 x 106 KOLF2-C1 cells were nucleofected (Lonza Amaxa 4D nucleofector ...
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bioRxiv - Microbiology 2022Quote: Sterile molten top NA (3 mL; 0.2 % SeaPlaque Agarose, Lonza) supplemented with CaCl2 and MgCl2 (both at 5 mM ...
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bioRxiv - Bioengineering 2024Quote: ... 21 μg/ml Bovine Pituitary Extract (BPE; Lonza, CC-4009), and 4 μM forskolin (Calbiochem ...
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bioRxiv - Cancer Biology 2023Quote: ... Mammosphere media was prepared with 475 mL of MEBM (Lonza) with 1x GlutaMAX (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza) and sedimented again at 10,500 rpm (∼20,000 × g ...
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bioRxiv - Bioengineering 2023Quote: ... 21 μg/ml Bovine Pituitary Extract (BPE; Lonza, CC-4009), and 4 μM forskolin (Calbiochem ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with ascorbic acid (75 μg/mL, Lonza, #CC-4398), human recombinant insulin (20 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 ml 100x L-glutamine (200 nM) (Lonza #BE17-605E), 5 mL 100x penicillin (5000 U/ml)/ streptomycin (5000 μg/ml) ...
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bioRxiv - Immunology 2024Quote: ... was used at a concentration of 0.75 U/mL (Lonza). Cells were analyzed for the presence of surface C3b (3E7/C3b ...
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bioRxiv - Bioengineering 2021Quote: Landing pad cell lines were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with the promoterless mCherry donor containing the matching attP at a dose of either 1000 ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 WT K562 were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Bioengineering 2021Quote: 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of a pEF-1α-PuroR-P2A-mCherry donor plasmid containing an upstream Cp36 attD site (pJT371) ...
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bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of the same Cp36 PuroR-P2A-mCherry donor ...
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bioRxiv - Cell Biology 2022Quote: Primary Aortic Smooth muscle cells from ATCC (PCS-100-012™) (AoSMC) were cultured with SmbM2 (Lonza) with 1x SmGMTM-2 SingleQuotsTM Supplement Pack (CC-4149).
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bioRxiv - Genetics 2020Quote: ... Parasites were subsequently resuspended in 100 μL nucleofector solution of the Basic Parasite Nucleofector Kit 2 (Lonza), combined with 50 μL plasmid solution containing 5-10 μg DNA ...
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bioRxiv - Biophysics 2021Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
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bioRxiv - Bioengineering 2019Quote: ... harvested by 0.25% trypsin/EDTA and resuspended in 100 μL nucleofector solution (Amaxa Biosystems/Lonza, Cologne, Germany) containing 5 μg CRISPR/Cas9 plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... After the cells reached 100% confluence the cells were exposed to adipogenic induction media (BulletKit, Adipogenesis, Lonza). The induction medium consisted of 1 mM dexamethasone ...
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bioRxiv - Cell Biology 2020Quote: ... After the cells reached 100% confluency the cells were exposed to osteogenic induction media (BulletKit, osteogenic, Lonza). The induction medium consisted of DMEM-LG ...
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bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μl Amaxa solution (Lonza Nucleofector 2b, program T-016), with 300 ng of the 11.6 kb LSR plasmid ...
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bioRxiv - Immunology 2021Quote: ... 2 × 106 cells were suspended in 100 µL buffer (P3 Primary Cell 4D-Nucleofector X Kit, Lonza), mixed with 4 µg DNA and transfected using the 4D-Nucleofector program DN-100 ...
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bioRxiv - Genomics 2022Quote: ... One million sorted Pro-E cells were resuspended with 100 μl solution P3 for primary cells (Lonza); preassembled RNP were then added to the cell suspension for nucleofection ...
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bioRxiv - Molecular Biology 2022Quote: ... The final solution (approximately 125 µL) was gently mixed and transferred to a 100 µL cuvette (Lonza). Electroporation was then performed according to the manufacturer’s instructions on the 4D-Nucleofector™ Core Unit (Lonza ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers of CHSE-214 cells were cultured until 90-100% confluent and detached by Trypsin EDTA (Lonza). 4 transfection reactions ...
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bioRxiv - Molecular Biology 2023Quote: One million Aire wild-type mTEC 3.10 cells were suspended in 100 μL of nucleofector solution (Lonza) containing 20 pmol of spCas9-GFP ...
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bioRxiv - Molecular Biology 2023Quote: ... were added to a nucleofection strip and the mixture pulsed with program EH-100 (Lonza 4D-Nucleofector). Cells were plated into 96-well plates for recovery with a fresh media change after 1 day ...
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bioRxiv - Biophysics 2023Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
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bioRxiv - Cell Biology 2020Quote: ... cells were serum starved for at least 13 h in 1% FBS (Atlanta Bio selected, Cat #S11110) or without FBS using endothelial cells growth basal medium (Lonza cat#cc-3121) instead of MCDB-131-complete media ...
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bioRxiv - Neuroscience 2020Quote: ... two pairs of sgRNA vectors and Cas9 nickase plasmid were transduced into the AD hPSCs by using Human Stem Cell Nucleofector® Kit 1 (Lonza VPH-5012). Three days after electroporation ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was then placed in a customized 35mm petri dish with sylgard-carved imaging chamber and covered with 1% low-melting agarose (Lonza #50101, Rockland, ME) for extra immobilization ...
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bioRxiv - Cell Biology 2020Quote: ... Conditioned media (containing EVs) was removed and the cells washed x 2 with PBS before trypsinisation using 1 × Trypsin-EDTA (Lonza, UK cat: T3924). Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5x105 iPSCs were resuspended in 20μL of P3 primary cell nucleofector solution plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) along with the RNP complex and nucleofected using Lonza 4D Nucleofector (program CA-137) ...
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bioRxiv - Immunology 2021Quote: ... in 1000 mL of phosphate buffered saline (PBS, Lonza, Basel, Switzerland) with 0.05% Tween ...
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bioRxiv - Physiology 2019Quote: ... and penicillin/streptomycin (50 U/mL of medium, Lonza, Verviers, Belgium). Dissociated cells were plated on poly-L-lysine pre-coated dishes fitted with a central glass bottom (MatTek Corporation ...