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Citations for Lonza :
1151 - 1200 of 1408 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 2×105 dissociated neurons were transfected with Lonza Nucleofector using the Basic Neuron SCN Nucleofector kit (Lonza, Basel, Switzerland). Transfected neurons were incubated for indicating days and processed for subsequent analyses.
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bioRxiv - Cancer Biology 2020Quote: ... E4ORF1-transduced primary human umbilical vein endothelial cells (HUVECs) were cultured using the EGM-2 Bullet Kit (Lonza, Germany). All cell lines were kept at 37°C and 5% CO2 in a fully humidified incubator and negatively tested for mycoplasma by PCR.
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing recombinant RBD of SARS-CoV-2 Spike (aa. 319-591) (GenBank accession no. AAP13567.1) were propagated in ProCho5 media (Lonza) containing glutamine ...
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bioRxiv - Cell Biology 2021Quote: Vero cells were transfected with 2 µg plasmid DNA in the Amaxa Nucleofector solution R (Lonza Bioscience, Rockville, MD), following the manufacturer’s instructions with program V-01 ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... and re-seeded onto 0.2% gelatin-coated 10-cm dishes with 5.0 × 105 cells per dish in Endothelial Cell Growth Medium (EGM; Lonza) containing 20 ng/mL VEGF ...
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bioRxiv - Immunology 2020Quote: ... Colons were incubated 2 times at 200 rpm in 40 mL HBSS + 0.1% BSA + 1% Penicillin-Streptomycin (PS, Lonza) + 5mM EDTA (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... expressing Cas9 and single sgRNAs targeting upstream and downstream regions of the lncRNA promoter/locus were co-transfected into 1.5 × 106 H1 hESCs using the Human Stem Cell Nucleofector Kit 2 (Lonza) and the Amaxa Nucleofector II (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... 2x overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS / MEM media and 0.4% (w/v) SeaPrep Agarose (Lonza) to achieve a semi-solid overlay ...
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bioRxiv - Cancer Biology 2020Quote: ... ON-TARGETplus non-targeting siRNA#2) were used for transient RNA interference experiments and transfected using Nucleofector technology (Lonza).
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bioRxiv - Genetics 2020Quote: ... 20 µg ssODNs and 4 µg pSpCas9(BB)-2A-GFP construct using Human Stem Cell Nucleofector Kit 2 (Lonza). For the generation of UE-RASGEF1A-int1-KO and PIK3C2B-int10-KO hPSC lines ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were nucleofected with 2μg of pcDNA3.1+ plasmid per reaction (Lonza-AMAXA program X-001, Nucleofector 2b). 24 hours after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Dry powder (2-3 mg) was loaded into an HPMC size 3 capsule (VCaps® Plus, Lonza, Morristown, NJ). The capsule was placed in a Plastiape high resistance RS00 DPI that was then attached to a Next Generation Impactor (NGI ...
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bioRxiv - Developmental Biology 2021Quote: ... HESCs were transfected with a total of 4ug DNA in a ratio 2:3 (gRNAs:donor template) using the Amaxa P3 Primary Cell 4D-Nucleofector Kit (Lonza). Puromycin was added 3 days after electroporation at a concentration of 0.5ug/ml ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg of assembled pGL3 plasmid were electroporated into 2.5×105 AIC-H9-hESCs 35 by 4D-Nucleofector (Lonza) using P3 Primary Cell 4D-Nucleofector X kit (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... HLFs were seeded in four wells of a 24-well plate in antibiotic free medium containing 2 % (Lonza, PromoCell) or 10 % (DZL ...
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bioRxiv - Bioengineering 2024Quote: ... HeLa cells (2×105 cells/condition) were electroporated using the SE Cell Line 96-well Nucleofector™ Kit (Lonza) and the EH-100 pulse code (Nucleofector 4D ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Umbilical Vein Endothelial Cells (HUVECs) were cultured in complete EGM-2 (cat# CC-3162, Lonza, Allendale, NJ, USA) and were not used beyond passage five for any experiments.
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bioRxiv - Cancer Biology 2024Quote: ... and (iv) the NDF-2 and NDF-3 cell lines (human neonatal dermal fibroblast cell lines, #CC-2509; Lonza Bioscience ...
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bioRxiv - Bioengineering 2023Quote: ... cAP-0001GFP) were cultured in 0.2% gelatin-coated T75 tissue culture flasks with EGM-2 medium (Lonza, CC-3162). The medium was changed every other day ...
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bioRxiv - Cell Biology 2022Quote: Human umbilical vein endothelial cells (HUVEC) were cultured between passage 3 and 6 in EGM-2 complete medium (Lonza)3,52–55 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting vector was used to generate a D.mel-2 stable cell line adapted to grow in suspension in serum-free Insect-Xpress medium (Lonza). In brief ...
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bioRxiv - Bioengineering 2022Quote: ... embryos were anesthetized by 30 mg/L tricaine-S (Western Chemical) and mounted in 2% low melting agarose (LONZA) for dorsal or lateral view in 35 mm Petri dishes with a glass bottom ...
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bioRxiv - Microbiology 2022Quote: ... and neonatal dermal microvascular endothelial cells (hDMVEC) were maintained as monolayer cultures in EBM-2 medium (Lonza, Walkersville, USA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Immunology 2022Quote: ... 2.4 μl CRISPR guide RNA (gRNA; key resource table) (100 μM) and 2 μl Cas9-NLS (80 μM) (Lonza) were mixed and incubated for 40 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: SK-N-BE (2) (ATCC: no. CRL-2271) cells were cultured in Dulbecco’s Modified Eagle Medium (Lonza, Cat# BW12614F), supplemented with heat-inactivated FBS (Hyclone ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fetal lung fibroblasts at passage 7 were cultured in FGM-2 lung fibroblast basal media (Lonza, #CC-3131) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... and mN designates the 2’-O-methylribonucleotides) was transfected into HAP1 cells (1.0 × 106 cells) by Nucleofector Kit V (Lonza) with a Nucleofector device (Lonza ...
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bioRxiv - Immunology 2023Quote: ... one plate was prepared with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were authenticated by Brca1/2-specific PCR-based genotyping (mouse)7,8 and they were regularly tested for mycoplasma contamination (Mycoalert, Lonza).
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bioRxiv - Biochemistry 2024Quote: ... the media supernatant was removed and we washed the cells with 2 ml of prewarmed 1xDPBS (Lonza, 17-512F). The PBS was then removed and cells were then incubated at 37°C in 250 μL of trypsin (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... dissociated neurons were electroporated with cDNA (2 µg) or siRNA (Table S2) using an Amaxa nucleofector (program-005, Lonza) and mouse neuron nucleofectorTM kit (VPG-1001 ...
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bioRxiv - Bioengineering 2024Quote: ... were seeded at 1 ×104 cells/cm2 over 0.5 cm2 sections of prosthesis held down by a PDMS ring inside 24-well plates with 1.5 mL of endothelial cell growth medium-2 (Lonza). After 2 h of adhesion ...
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bioRxiv - Systems Biology 2024Quote: ... HUVECs were plated onto 0.2% gelatin-coated plates and grown in sterile endothelial growth medium (EGM-2 BulletKit, Lonza) containing endothelial basal medium (EBM-2) ...
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bioRxiv - Physiology 2024Quote: ... Red blood cells were lysed in 2 ml of sterile ammonium–chloride–potassium lysis buffer (ACK Lysing Buffer, Lonza) for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary human umbilical vein endothelial cells (HUVECs) were maintained in an EGM-2 Bullet Kit (CC-3162, Lonza, USA). AS1842856 (Cat#HY-100596 ...
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bioRxiv - Genetics 2024Quote: ... A20 B lymphoma cells were co-transfected with 2 μg of the firefly luciferase plasmids and 0.25 μg of pRL-Renilla luciferase plasmid using Nucleofection (Lonza) and analyzed at 24 hours after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... The next day the chips were perfused with endothelial growth medium (Lonza, EGMTM-2 BulletKitTM, Cat. no. CC-3162) in the basal channel and Caco-2 growth medium (15% FBS in DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... half of the cell culture media was replaced with 2x concentrated preadipocyte growth medium (PGM-2, PT-8002, Lonza) and media was changed on d15 and d20 ...
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bioRxiv - Developmental Biology 2020Quote: ... about 8 × 105 iPSCs were transfected with 5 μg of plasmids with Lonza Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012) on a Nucleofector 2b device (Lonza ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were electroporated with either CFP-IRES or RBP-IRES-CFP vector (5 µg) using a 4D-Nucleofector (Lonza, program DS-112) in 16-well stripes (500,000 cells/well ...
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bioRxiv - Systems Biology 2020Quote: Single-cell suspensions were pelleted at 400 x g for 5 min and washed once with 10 mL mammary epithelial basal medium (MEBM; Lonza CC-3151). For each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 µg of PX459 gRNA vector (5’-GACTCCAGTCTTTCTAGAAGA-3’) were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-100) using program A-30 in the XRep ...
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bioRxiv - Biophysics 2021Quote: ... ATCC-CRL 10317) were grown at 37°C and 5% CO2 in Mammary Epithelial Cell Growth Basal medium (MEBM from Lonza Pharma & Biotech), supplemented with 5% Horse Serum (HS ...
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bioRxiv - Microbiology 2021Quote: ... Recovered cells were further depleted of red blood cells by resuspending cells with 5-ml of AKC lysis buffer (Lonza, Walkersville, MD). Following 5 min incubation at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... all cells were cultured at 37°C in a humidified 5% CO2 incubator in minimum essential medium Eagle α (Lonza; BE12-169F) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... cells were transduced with Adenovirus-GFP (AV-Gfp) or Adenovirus-mHilpda (AV-Hilpda) at 5 × 106 IFU/mL media in DMEM (Lonza, Verviers, Belgium) supplemented with 10% fetal calf serum (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with ACK Lysing Buffer (10-548E, Lonza Bioscience, Switzerland) to remove red blood cells ...
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bioRxiv - Microbiology 2022Quote: ... schizonts purified from an overnight culture of PbDiCre parasites were transfected with 5–10 µg of linearised plasmid by electroporation using the AMAXA Nucleofector device (Lonza, program U033), as previously described (Janse et al. ...
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bioRxiv - Immunology 2022Quote: ... 5×106 cells were mixed with RNPs and transferred without forming bubbles to a 16-well Nucleocuvette© Strip (Lonza, V4XP-3032). Unless otherwise stated ...