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Citations for Lonza :
901 - 950 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and routine mycoplasma contamination checks were conducted using the MycoAlertTM Mycoplasma Detection Kit (Lonza, Switzerland).
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bioRxiv - Bioengineering 2024Quote: ... The cells were resuspended in 20 μL P3 Nucleofector Reagent (NFR) per manufacturer’s instructions (P3 Primary cell 4D-Nucleofector, Lonza). Transfection reaction was prepared by adding 1.5 μg chemically modified SpCas9 mRNA (CleanCap® ...
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bioRxiv - Biophysics 2024Quote: ... 0.5 μg of F-tractin-mScarlet plasmid and 0.75 ug of Zyxin-mNeonGreen plasmid were mixed with solution SE from SE Cell Line 4D-Nucleofector S Kits (Lonza). 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).
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bioRxiv - Cell Biology 2024Quote: ... ACK lysing buffer (Lonza, Walkersville ...
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bioRxiv - Cell Biology 2024Quote: Normal human lung fibroblasts (HLFs) (Lonza) were cultured as previously described (15) ...
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bioRxiv - Cell Biology 2024Quote: The primary HUVECs (human Umbilical Vein Endothelial Cells) from Lonza (CC-2519, USA) were cultured in EndoGRO (EMD Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... as well as two sgRNA plasmids using program CD-118 of the 4D Nucleofector system (Lonza). hiPSCs were plated onto 10 cm Geltrex-coated dishes in mTeSR Plus/Y-27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 x 106 REH cells were transfected with 200 pmol of each siRNA pool (siCtrl, siH1-0_1, siH1-0_2) in the 100 µl nucleocuvette format using the 4D Nucleofector system (Lonza, SF solution, program DS-150).
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bioRxiv - Biophysics 2024Quote: ... Young adult worms were placed on a thin layer of 2% agarose (Lonza, SeaKem LE agarose) prepared on a 24 × 55-mm coverslip (Matsunami Glass ...
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bioRxiv - Bioengineering 2024Quote: ... were cultured on a 0.1% gelatin-coated tissue culture dish at 37°C and 5% CO2 in Endothelial Cell Growth Medium - 2(EGM-2, Lonza, Basel, CH). HUVECs were transduced with mCherry lentiviral pseudovirus (pCDH-CMV-mCherry-T2A-Puro ...
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bioRxiv - Biophysics 2024Quote: ... MCF7 cells were electroporated with 1µg of DNA plasmid HER2-GFP from Sino biologicals (ref HG10004-ACG) with Nucle-ofector 2b device (Lonza), and selected by antibiotic hygromycine ...
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bioRxiv - Biophysics 2024Quote: ... were purchased from Lonza Bioscience (Walkersville ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.25 μg/mL amphotericin B and 25 mM HEPES (both from Lonza), 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with Ca2+Mg2+ free-HBSS (Lonza) and sliced into 1-3 mm3 fragments with a sterile scalpel ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells are cultured in ABMTM Basal Medium (Lonza, cat. CC-3187) and AGMTM SingleQuotsTM Supplements (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfections were performed using nucleofection (Lonza, 4D- Nucleofector™ X-unit) with solution P3 and program CA-137 in a small (20ul ...
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bioRxiv - Cancer Biology 2024Quote: ... 200ng of pMaxGFP (Lonza), and 3ug of ssODN donor (Rifatbegovic ...
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bioRxiv - Cancer Biology 2024Quote: ... Mycoplasma testing was performed on all cells when they were thawed and semi-regularly thereafter using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza LT07-703). Independent experiments were performed on cells treated with siRNA and/or compounds from separate passages of each cell line ...
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bioRxiv - Cancer Biology 2024Quote: ... and were screened for mycoplasma (MycoAlert kit, Lonza, Basel, Switzerland). HCI-10 PDX tumor fragments were provided by Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... OS-17 cells electroporated with SE buffer (Lonza) and the CM-104 program had the highest percentage of GFP+ cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and electroporated using the 4D-Nucleofector system (Lonza) program CM-104 in the presence of electroporation enhancer (IDT) ...
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bioRxiv - Cell Biology 2024Quote: ... The pelleted cells were resuspended with 100μl electroporation buffer (Lonza, Cat #: VPG-1001) containing 0.3 nmol nontargeting control siRNA (siRNA scramble ...
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bioRxiv - Cell Biology 2024Quote: ... Cat #:SR405443) and transferred to a 2.0 mm electroporation cuvette and electroporated with Nucleofector II (Lonza) following the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell cultures were confirmed mycoplasma-free with MycoAlert™ Mycoplasma Detection Kit (Lonza), using luminometer Centro LB960 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human umbilical vein endothelial cells (HUVEC; C2519A) and normal human lung fibroblasts (nhLF; CC-2512) were purchased from Lonza and cultured in coated flasks with 50 μg/ml rat tail collagen I (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
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bioRxiv - Cell Biology 2024Quote: ... Electroporation was conducted using Amaxa Nucleofector 2b Device (Lonza) with Nucleofector program B-016 ...
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bioRxiv - Cancer Biology 2024Quote: ... They are also tested biannually for mycoplasma contamination (Latest negative screen February 2024, Lonza LT07-318).
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bioRxiv - Cancer Biology 2024Quote: ... 0.25×106 cells were electroporated using two different electroporation kits and 4D-NucleofectorTM device (Lonza) to deliver 1 µg of pDNA encoding GFP ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were validated by STR profiling and routinely tested for mycoplasma (Lonza).
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bioRxiv - Cancer Biology 2024Quote: ... and human mesenchymal stem cells (hMSC, Lonza, cat. PT-2501) were purchased from Lonza (Walkersville ...
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bioRxiv - Cancer Biology 2024Quote: ... Pooled donor human umbilical vein endothelial cells (HUVEC, Lonza, cat. CC-2519) and human mesenchymal stem cells (hMSC ...
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bioRxiv - Cancer Biology 2024Quote: ... were purchased from Lonza (Walkersville, MD).
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bioRxiv - Neuroscience 2024Quote: ... embryonic bodies were collected and used to generate myeloid factories by growing them in X-VIVO 15 medium (Lonza, Basel, Switzerland) supplemented with 2 mM Glutamax ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transfected with siRNA Ackr3 (Mouse) SMARTPool (Dharmacon) at a final concentration of 100nM in combination with 0.5 μg of pmaxGFPTM (LONZA) and Lipofectamine Stem Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... in 2 ml of EB Differentiation medium (X-Vivo 15 (Lonza), 2 mM Glutamax (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 0.5% penicillin/streptomycin (Lonza), 2% B27 (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... each consisting of 28 chambers connected to an independent computer.All tastants used were solved in water 1% low melt agarose (Lonza, ref. 50100). To transfer files to each chamber ...
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bioRxiv - Neuroscience 2024Quote: ... then nucleofected with 1ug vector using Amaxa Mouse NSC Nucleofection kits (Lonza catalog #VPG-1004). Transfection efficiency was assessed by quantifying GFP expression via CytoFLEX flow cytometer (Beckman).
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bioRxiv - Cell Biology 2024Quote: ... expanded in submerged culture for one or two passages in bronchial epithelial growth medium (BEGM, Lonza, Walksville, MD), and then seeded on Transwell membranes (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were authenticated by Multiplex Cell Line Authentication (MCA) and were tested for mycoplasma by MycoAlert Mycoplasma Detection Kit (Lonza). HEK293T cells were transfected with the plasmids using 1mg/ml polyethylenimine ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 x 106 hiPSCs were resuspended in 100 µl P3 solution with supplement (Lonza, #V4XP-3024) and transfected with 2.5 µg linearized RUNX1 HDR template and 4 µg each of pCW-Cas9 plasmid (Addgene ...
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bioRxiv - Biophysics 2024Quote: ... 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).
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bioRxiv - Bioengineering 2024Quote: Human umbilical vein endothelial cells (HUVECs, Lonza, Basel, CH) were cultured on a 0.1% gelatin-coated tissue culture dish at 37°C and 5% CO2 in Endothelial Cell Growth Medium - 2(EGM-2 ...
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bioRxiv - Biophysics 2024Quote: Cells were routinely tested for the presence of mycoplasma using the mycoALERT kit (Lonza). None of the cell lines in this study were found in the database of commonly misidentified cell lines maintained by ICLAC and NCBI Biosample.
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bioRxiv - Bioengineering 2024Quote: ... The pelleted cells were resuspended in 100-200 µl of ACK lysis buffer (BioWhittaker, Lonza) for 5 min at room temperature and quenched with the addition of equal volume of 5 mM EDTA in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were routinely screened throughout the study and found to be negative for mycoplasma with the MycoAlert detection kit (Lonza).
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bioRxiv - Bioengineering 2024Quote: ... The agarose pad was prepared with SeaPlaque GTG Agarose (Lonza) and SOB supplemented with surfactant and SYTOX Blue ...
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bioRxiv - Biophysics 2024Quote: ... were expressed in Sf21 insect cells grown in Insect-XPRESS medium (Lonza) at 27 °C for 66 hours ...
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bioRxiv - Bioengineering 2024Quote: ... The transfection reaction was transferred into P3 Primary cell cuvette and electroporated with EO-115 program on a Lonza 4D Nucleofector (Lonza). The cells were rested in the cuvette without disturbing them for 15 minutes at RT ...