Labshake search
Citations for Lonza :
851 - 897 of 897 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: Human isogenic ESCs containing different lengths of CAG repeats in the HTT Exon1 locus (HD-RUES2 6) were nucleofected using the Cell Line Nucelofector II (Kit L from Lonza, Walkersville, MD) by applying the B-016 program ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 199 (M199) medium (3: 1) supplemented with 10% fetal bovine serum (FBS),10 mM glutamine and penicillin-streptomycin (all from Lonza, Basel, Switzerland) and their purity was verified with the fibroblast marker TE-7 (Merck ...
-
bioRxiv - Immunology 2022Quote: ... MV-4-11 (ATCC #CRL-9591), mBMDMs (were a kind gift from Linfa Wang, Duke-NUS, Singapore) and normal bronchial epithelial cells (NHBE, Lonza #CC-2541) were cultured according to manufacturer’s protocols ...
-
bioRxiv - Biophysics 2021Quote: ... ATCC-CRL 10317) were grown at 37°C and 5% CO2 in Mammary Epithelial Cell Growth Basal medium (MEBM from Lonza Pharma & Biotech), supplemented with 5% Horse Serum (HS ...
-
bioRxiv - Cell Biology 2021Quote: Liver spheroids were prepared as previously described (Leite et al., 2016) except spheroids were formed from primary rat hepatocytes (Lonza, cat# RSCP01) and primary human HSCs ...
-
bioRxiv - Cancer Biology 2020Quote: ... human astrocytoma CCF-STTG1 (ATTC CRL-1718) and human glioblastoma cell line U87MG (ATCC HTB-14) purchased from Lonza (Lonza Walkersville. Inc.) and American Type Culture Collection (ATCC) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Immortalized human bronchial epithelial BEAS-2B cells were purchased from ATCC and cultured in BEGM bronchial epithelial cell growth medium (Lonza #CC-4175). All the cells were grown at 37°C with 5% CO2.
-
bioRxiv - Immunology 2021Quote: Primary BEAS-2B (ATCC® CRL-9609TM) (normal human airway epithelial cells) as well as all the basal media and growth supplements were obtained from Lonza (Walkersville, MD). Cells were cultivated according to the instructions of the manufacturer on plastic dishes or flasks (BD Bioscience ...
-
bioRxiv - Systems Biology 2022Quote: ... Early passage HUVECs were cultured in EGM™2- Bulletkit™ medium with growth supplements CC-3156 & CC-4176 purchased from Lonza. At 80% confluency ...
-
bioRxiv - Microbiology 2021Quote: ... All cell lines were routinely tested for the absence of mycoplasma using the MycoAlert Plus Mycoplasma Detection Kit from Lonza (Walkersville, MD).
-
bioRxiv - Immunology 2020Quote: ... 2 million L1.2 cells were transfected with 2 µg of plasmid using the SG Cell Line transfection kit and a 4D-Nucleofector X (both from Lonza, Walkersville, MD) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... All cell lines were routinely tested to confirm absence of mycoplasma using the MycoAlert Plus Mycoplasma Detection Kit from Lonza (Walkersville, MD).
-
bioRxiv - Synthetic Biology 2022Quote: ... human aortic endothelial cells (CD31+) from were maintained in EGM™-2 Endothelial Cell Growth Medium-2 Bullet Kit™ (Cat # CC-3162; Lonza) and EGM™ −2 MV Microvascular Endothelial Cell Growth Medium-2 Bullet KitTM (Cat #CC-3202 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The culture medium or the DNA harvested from the cultures were tested for mycoplasma contamination using a MycoAlert Mycoplasma Detection Kit (Lonza, LT07-418) or e-Myco PLUS PCR Detection Kit (BOCA scientific ...
-
bioRxiv - Bioengineering 2024Quote: Normal adult human dermal fibroblasts (NHDF-Ad) and human epidermal keratinocytes-adult (NHEK-Ad) were purchased from Lonza (CC-2511 Walkerville, MD), and SiHa cells were purchased from ATCC (ATCC® HTB-35™ ...
-
bioRxiv - Cancer Biology 2024Quote: Fresh single cell suspension remaining from PDOTS processing (S3) was grown in SmGM-2 Smooth Muscle Cell Growth Medium (Lonza, CC-3181) supplemented with SmGM-2 SingleQuots Supplement Pack (Lonza ...
-
bioRxiv - Pathology 2024Quote: ... and their respective optimal culture media (SmBm2/EBM2 basal medium for experiments, and SmGm2/EGM2 complete medium for expansion) were purchased from Lonza (Walkersville, MD). Primary cells between passages 4-8 were cultured at 37°C with 5% CO2 as previously described.30,31 For subculture ...
-
bioRxiv - Cell Biology 2024Quote: The NK-92 cell line was acquired from the American Type Culture Collection and cultured in X-VIVO 10 (Lonza, 04-380Q) supplemented with 20% fetal bovine serum (FBS ...
-
A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... with SmGM until confluency and then in SmBM (SmGM without complement) + 2% (v/v) FBS and antibiotics from the Bulletkit for SmGM (CC-4149, Lonza, Basel, Switzerland). SEP was added once at the beginning of the experiment at 100 µg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... MCF10A cells (female human mammary epithelial cells) were purchased from ATCC (CRL-10317) and cultured as recommended by ATCC in MEGM (Lonza CC-3150) supplemented with bovine pituitary extract ...
-
bioRxiv - Cell Biology 2023Quote: ... The linearized construct and the purified schizonts were mixed with Nucleofector solution from an Amaxa human T cell Nucleofector Kit and electroporated using the Amaxa Nucleofector II device (Lonza, Köln, Germany). Directly after transfection 50 µl RPMI-1640 complete was added to the transfection reaction followed by intravenous injection into one SWISS mouse ...
-
bioRxiv - Molecular Biology 2023Quote: ... and NIH-3T3 (CRL-1658) were obtained from ATCC and were tested for mycoplasma at least quarterly using MycoAlert (Lonza LT07-318). U-2OS cells stably expressing a TetOn 3G transactivating protein and MS2-hairpin binding protein fused to YFP were previously described5 ...
-
bioRxiv - Genomics 2024Quote: ... we generated Unrelated Control 2 iPSC using purchased human dermal fibroblasts from a healthy unrelated male (CC-2511, Lot No. 0000293971, Lonza, Basel, Switzerland). We conducted fibroblast collection and culturing ...
-
bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVEC) were purchased from ATCC (Manassas, VA, USA) and cultured in EGM-2 Endothelial Cell Growth Medium-2 BulletKit (Lonza, Basel, Switzerland) at 37°C with 5% CO2 and 95% air conditions until passage 5 ...
-
bioRxiv - Cell Biology 2024Quote: ... In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit). T98G cells were purchased from ATCC ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 37°C with 5%CO2 using EGM-2 medium supplemented with SingleQuots from Lonza (CC-3156 & CC-4176). Cells were passaged using 0.25% trypsin EDTA every 2–3 days ...
-
bioRxiv - Immunology 2024Quote: ... All iPSC-derived cell lines were tested and negative for mycoplasma contamination using the MycoAlert Mycoplasma test kit from Lonza (#LT07-318).
-
bioRxiv - Immunology 2024Quote: ... Human primary fetal tissue was only used after it was confirmed negative for mycoplasma contamination using the MycoAlert Mycoplasma test kit from Lonza (#LT07-318).
-
bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
-
bioRxiv - Cell Biology 2024Quote: ... were obtained from Stephen Elledge’s laboratory at Harvard Medical School73 and cultured in MEGM™ Mammary Epithelial Cell Growth Medium (Lonza CC- 3150). In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit) ...
-
bioRxiv - Genomics 2024Quote: ... Approximately 12 million cells per replicate were spun down and resuspended in nucleofection solution from the Lonza SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2012) and nucleofected in 12 reactions with 5 μg pLJK07 or pLJK08 per 1 million cells according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 OVCAR8 landing pad cells were resuspended in 20 µl of nucleofector solution combined with supplement at 4.5:1 from the Amaxa SE Cell Line 4D-Nucleofector X Kit S (Lonza, Cat #V4XC-1032), before addition of 5 µl RNP complex and 1 µl of Electroporation enhancer ...
-
bioRxiv - Immunology 2021Quote: ... Vero cells were acquired from the American Type Culture Collection (ATCC #CCL-81; Manassas, VA, USA) and cultured in DMEM (Lonza, Walkersville, MD, USA) containing 2% FBS (SAFC Biosciences ...
-
bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... human astrocytoma CCF-STTG1 (ATTC CRL-1718) and human glioblastoma cell line U87-MG (ATCC HTB-14) purchased from Lonza (Lonza Walkersville. Inc.) and American Type Culture Collection (ATCC) ...
-
bioRxiv - Biophysics 2023Quote: ... we transfected HeLa cells with SNAP-tagged CENP-A (generous gift from Dan Foltz) in combination with either empty vector or GFP-CENP- C using the Amaxa Nucleofector kit R (Lonza Bioscience, Walkersville, MD) per instructions.
-
bioRxiv - Molecular Biology 2023Quote: Human primary pulmonary artery ECs (HPAECs) were obtained from ATCC and grown on gelatin-coated dishes in Endothelial Cell Basal Medium-2 (Lonza, Catalog # CC-3156) supplemented with EGM-2 SingleQuots Supplements (Lonza ...
-
bioRxiv - Cell Biology 2024Quote: The commercially available human myoblast (hMB) stock isolated from a healthy 35-year-old female (CC-2580, lot 0000483427; Lonza, Walkersville, MD, USA) was used [7,10] ...
-
bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
-
bioRxiv - Cell Biology 2024Quote: ... The cells were used from passages 5-9 for the experiments and were cultured in endothelial cell growth Medium-2 (EGM™-2) Bulletkit™ (Lonza Bioscience) on pretreated tissue culture 6-well plate (VWR international) ...
-
bioRxiv - Neuroscience 2024Quote: Induced pluripotent stem cells (iPSCs) from LiPSC-GR1.1 (RRID:CVCL_RL65, Male, Hispanic, normal, <1D, umbilical cord blood, Lonza Inc. via NIH RMP, MCB p28) and NCRM5 cell lines (RRID ...
-
bioRxiv - Cancer Biology 2024Quote: ... D2.A1 cells stably expressing luciferase (a gift from Dr. William Schiemann, Case Western Reserve University) were cultured in DMEM media (Lonza, catalog# BW12-604F) supplemented with 10% (v/v ...
-
bioRxiv - Developmental Biology 2024Quote: ... the 4 specific guide mixes were transferred into 100 μl of electroporation buffer (82 μl electroporation buffer + 18 μl supplement from the Lonza rat nucleofector kit). Additionally ...
-
bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...
-
bioRxiv - Bioengineering 2020Quote: Human Umbilical Vein Endothelial cells (HUVECs) and Human Aortic Endothelial Cells (HAECs) were purchased from Lonza (Cat. No. CC-2517, Cat. No. CC-2535). The culture medium was the endothelial basal medium-2 (EBM-2 ...
-
bioRxiv - Bioengineering 2020Quote: ... The culture medium was the endothelial basal medium-2 (EBM-2) supplemented with endothelial growth medium (EGM-2) BulletKit from Lonza (Cat. No. CC-3162). Cells were grown in tissue culture flasks and maintained in humidified atmosphere at 37°C and 5% CO2 ...
-
bioRxiv - Bioengineering 2023Quote: The murine myoblast cell line C2C12 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was tested for mycoplasma (MycoAlert™ Mycoplasma Detection Kit, Lonza AG, Basel, Switzerland). Cells were cultured in monolayer at 37 °C in a 5% CO2-containing humidified atmosphere in complete growth medium (GM) ...