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Citations for Lonza :
701 - 750 of 897 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Two million WTC iPSC were nucleofected using an Amaxa nucleofector 2B and the Nucleofector Kit C (both from Lonza), 0.5 µg of each AAVS1 TALEN pair and 1 µg of the donor plasmid (see Figure S4) ...
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bioRxiv - Bioengineering 2022Quote: NK-92 cell line was acquired from the American Type Culture Collection and maintained in X-VIVO 10 (Lonza) supplemented with 20% FBS and 0.02 mM folic acid (Millipore Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... rat hippocampal neurons from E18 embryos were electroporated before plating with 1.5 μg of DNA using Nucleofector system (Lonza). For DNA-PAINT ...
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bioRxiv - Biochemistry 2020Quote: ... The complexes were separated from unbound species by electrophoresis on a 0.7% nondenaturing gel using a SEAKEM GTG agarose solution (Lonza) made with 1× TBE buffer ...
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bioRxiv - Microbiology 2022Quote: Human liver chimeric mice were generated by transplantation of approximately 106 primary human hepatocytes (donor L191501 from Lonza, Switzerland) into homozygous uPA+/+-SCID mice as previously described (79) ...
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bioRxiv - Immunology 2022Quote: Cryopreserved CAR T cells produced from heathy donors in the Seattle Children’s GMP production facility were thawed and cultured overnight in XVIVO media (Lonza) supplemented with IL-2 at 4.6ng/ml ...
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bioRxiv - Physiology 2021Quote: Spleens were harvested postmortem from 28-29-week-old female WT or GαzKO mice and collected in RPMI medium (Lonza). Cell isolation and flow cytometry procedures were conducted as previously described (17) ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were free from mycoplasma contamination after testing with the MycoAlert™ PLUS mycoplasma detection kit (Lonza) (LT07-701).
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bioRxiv - Microbiology 2021Quote: Primary human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Cat# CC-2540, male; Lonza; Donor 1) or derived directly from a patient at Cambridge University Hospitals NHS Trust (Research Ethics Committee Reference 19/SW/0152 ...
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bioRxiv - Neuroscience 2022Quote: ... and sgRNAs against LRRK2 (crRNAs ordered from IDT) were introduced into iPSCs via nucleofection (Lonza P3 kit # V4XP-3032) as described before(75) ...
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bioRxiv - Biophysics 2023Quote: ... tsa-201 cells were transfected via Lipofectamine 2000 24-48 hours prior to experiments with 1 μg pCMV6-SCN9A (gift from Dr. Christoph Lossin) and 0.5 μg pMaxGFP (Lonza) for identification of transfected cells ...
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bioRxiv - Bioengineering 2024Quote: Commercially available primary adult human mesenchymal stem cells (hMSCs) from Donor 47506 were used (Lonza Group Ltd., Basel, Switzerland). Cells were cultured at passage 3 and seeded in triplicate in hMSC Growth Medium (MSCGM™ Mesenchymal Stem Cell Growth Medium ...
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bioRxiv - Neuroscience 2023Quote: ... MECP2 knock out HAP1 cells from Horizon Discovery (Cat: HZGHC001102c010, RRID: CVCL_SX72) were cultured in IMDM ((Lonza, 12-722F) supplemented with 10% FBS (VWR 97068-085 ...
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bioRxiv - Bioengineering 2023Quote: ... Endothelial cells (ECs) were derived from CD34+ stem cells in endothelial cell growth medium (EGM; Lonza cat# CC-3162) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Bioengineering 2024Quote: ... Human primary bone marrow MSCs derived by plastic adherence of mononucleated cells from human bone marrow aspirate donors (Lonza) and were cultured in α-minimal essential medium (αMEM ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were mixed with complete p3 electroporation solution (from a Lonza 4D nucleofection kit; Lonza Group AG, Basel, Switzerland) followed by addition of the RNP complex and 500 pmoles of donor ssODNA (Alt-R HDR ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells from a single donor were amplified and differentiated into air-liquid interface cultures as recommended by Lonza and described in (49) ...
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bioRxiv - Immunology 2023Quote: Low passage primary human bronchial epithelial cells (HBEC) from healthy adult donors were obtained commercially (Lonza, Walkersville, MD, USA) and cultured at air-liquid interface according to the manufacturer’s instructions (Stem Cell Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM with 4.5 g/L glucose and L-glutamine) from Lonza (Basel, Switzerland) with 10 % v/v fetal bovine serum (Gibco 16000-044) ...
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bioRxiv - Microbiology 2024Quote: ... Heidelberg University, Germany) and MRC5 cells (purchased from ATCC, CCL-171) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Lonza) supplemented with 8% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Human umbilical vein endothelial cells (HUVEC; C2519A) and normal human lung fibroblasts (nhLF; CC-2512) were purchased from Lonza and cultured in coated flasks with 50 μg/ml rat tail collagen I (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... were obtained directly from umbilical cords donated by Bluets Maternity Hospital (Pierre Rouquès Hospital, Paris - Dr. Jessica Dahan Saal) or purchased from Lonza. These cells were cultured in flasks pre-coated with 10 µg/ml human fibronectin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Modified MTEC culture media is comprised of small airway basal media (SABM) with selected components from SAGM bullet kit (Lonza) including Insulin ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-sense RNA probe against Gdnf exons was transcribed and hybridized on thick sections derived from P4 kidneys embedded in 4% low melting agarose (NuSieve GTG, Lonza). BM purple was used for colorimetric reaction.
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bioRxiv - Microbiology 2020Quote: ... the RNP complexes were added to 1×105 cells in 40 μl of P3 nucleofection buffer from the 4D-Nucleofector X kit (Lonza). Half of the mixture was loaded to each well of a 16-well nucleofection cassette and nucleofected using the using E0-100 program with the 4D-Nucleofector Lonza Amaxa ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 90 μL of nucleofection solution (16.2 μL of Supplement solution mixed with 73.8 μL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza) was mixed thoroughly with the cell pellet ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ cells were purified from the apheresis PBMC collection via immunoabsorption and cultured overnight in at 37°C in X-VIVOTM 10 (Lonza) supplemented with 1% HSA (Baxter ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... Each chamber was connected to a reservoir that was pre-filled with 15 mL of warm serum-free EGM-2 (endothelial cell growth medium-2 without serum added from the kit, Lonza). ECFCs were harvested and resuspended in serum-free EGM-2 and added to the reservoirs to reach an overall cell density of 125,000 cells/mL ...
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bioRxiv - Genomics 2020Quote: ... A2lox ESCs (a gift from Michael Kyba) were transduced with 20 μg of each plasmid using the 4D-Nucleofector (Lonza), mouse ES program and P3 primary cell kit ...
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bioRxiv - Bioengineering 2020Quote: ... Human umbilical vein endothelial cell (HUVEC, C25271) and endothelial growth medium (EGM-2MV, CC4147) were purchased from LONZA (Basel, Switzerland). Rabbit anti-perilipin antibody (9349S ...
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bioRxiv - Cancer Biology 2020Quote: ... Both cell types were cultured and used in TOC devices for up to seven passages in complete growth medium from Lonza (FGM-2 for NHLFs and EGM-2 for ECFC-ECs) ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl or 100 μl of homogenate from groups was plated on both YPD agar supplemented with 250 U/ml ,250 μg/ml penicillin-streptomycin (Lonza), 30 μg/ml gentamicin sulfate (BioWhittaker ...
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bioRxiv - Microbiology 2021Quote: ... Specific cells used were human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Lonza; Cat# CC-2540, male); human bronchial epithelial cells from a male smoking donor undergoing bronchoscopy for a non-cancer indication ...
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bioRxiv - Microbiology 2020Quote: ... were isolated from a 79-year-old Caucasian female and were maintained in bronchial epithelial growth media (Lonza, CC-3171) supplemented with BEGM SingleQuots as per the manufacturer’s instructions (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... fumigatus collected from three 10-cm plates grown for 7 days at 37°C were germinated with shaking in RPMI 1640 (Lonza) liquid media supplemented with glucose to a final concentration of 2% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Microbiology 2020Quote: ... were isolated from a 79-year-old Caucasian female and were maintained in bronchial epithelial growth media (Lonza, CC-3171) supplemented with BEGM SingleQuots as per the manufacturer’s instructions (Lonza ...
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bioRxiv - Neuroscience 2020Quote: Primary human cortical astrocytes purified from 17-18 weeks gestation human fetuses of indeterminate sexes were used in this experiment (Lonza). Astrocytes were expanded using the astrocyte growth medium bulletkit according to the manufacturer’s instructions at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... was introduced into 5 x 105 H9 ESCs (passage number 49) from a subconfluent culture using a 4D-Nucleofector System with X Unit (Lonza) and P3 Primary Cell 4D-Nucleofector Kit S (Lonza ...
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bioRxiv - Developmental Biology 2022Quote: Primary HDLECs were isolated from neonatal human foreskins as described previously (74) and cultured from passages 5 – 7 on fibronectin-coated plates with EGM-2 MV growth media (Lonza) supplemented with human VEGF-C (R&D) ...
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bioRxiv - Bioengineering 2022Quote: ... MSC aggregates of 5000 MSCs were cultured in MSC-BMTM or MSC-GMTM (both from Lonza Group Ltd, Basel, Switzerland) using the same technology and monitoring of aggregate formation was performed by phase contrast microscopy (IMT2 ...
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bioRxiv - Bioengineering 2022Quote: ... and NP-C1 patient fibroblasts (GM18393 and GM17919A) were purchased from the Coriell Cell Repository (Table S1) and cultured in DMEM (Lonza) supplemented with 20% FBS (Bovogen) ...
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bioRxiv - Cell Biology 2022Quote: ... human astrocytoma CCF-STTG1 (ATTC CRL-1718) and human glioblastoma cell line U87-MG (ATCC HTB-14) purchased from Lonza (Lonza Walkersville ...
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bioRxiv - Cancer Biology 2022Quote: ... siControl and siIRAK4 were purchased from Horizon Discovery and transfected into human AML samples using Amaxa Nucleofector Kit T (Lonza) (program number G-016 ...
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bioRxiv - Cell Biology 2021Quote: ... Both endothelial cell lines (HUVEC, hPAEC) were grown in EBM-2 media with EGM-2 bullet kit (purchased from Lonza). No additional serum was used for cell culture ...
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bioRxiv - Microbiology 2020Quote: ... The virus growth media was either Minimum Essential Medium – Eagle with Earle’s BSS (MEM Eagle EBSS) from Lonza (Rochester, NY) or Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... and maintained in D10 media or endothelial growth medium 2 (EGM-2) using the EGM-2 bullet kit from Lonza, respectively ...