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Citations for Lonza :
6751 - 6800 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were then nucleofected using a 4D-Nucleofector™ with X-Unit (V4XP-4032 and V4XP-9096, Lonza, Basel, Switzerland). Up to three RNP complexes in 9 µL were used per reaction ...
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bioRxiv - Immunology 2021Quote: ... Pellets were resuspended in Primary Cell 4D-Nucleofector™ X Kit S (Lonza) buffer solution at a cell concentration of 1–4×106 cells in 20 µL ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed with ACK (Lonza, Walkersville, MD) to remove erythrocytes ...
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bioRxiv - Immunology 2021Quote: ... Naïve T cells expressing Cas9 were washed once with 1 mL pre-warmed recovery medium (Mouse T Cell Nucleofector Medium, Lonza) before electroporation ...
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bioRxiv - Immunology 2021Quote: ... 2 × 106 cells were suspended in 100 µL buffer (P3 Primary Cell 4D-Nucleofector X Kit, Lonza), mixed with 4 µg DNA and transfected using the 4D-Nucleofector program DN-100 ...
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bioRxiv - Immunology 2021Quote: ... RAW264.7 cells were cultured in RPMI 1640 (Cat: 12-702F from Lonza Pharma & Biotech, USA) supplemented with 10% inactivated fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... PHHs were commercially obtained (Lonza, Basel, Switzerland). Cryopreserved PHH were thawed as recommended by the manufacturer.
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bioRxiv - Immunology 2021Quote: ... cells were washed with PBS and 1 ml of fresh Hepatocyte Culture Medium (HCM) was added per well (Lonza). At 6 or 72 hpi ...
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bioRxiv - Immunology 2021Quote: ... and pulmonary artery smooth muscle cell (PASMC, Lonza, CC-2581) were seeded at 200,000 cells/well in twelve-well plates and cultured in media as specified by manufacturers ...
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bioRxiv - Immunology 2021Quote: ... normal human lung fibroblast (NHLF, Lonza, CC-2512), and pulmonary artery smooth muscle cell (PASMC ...
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bioRxiv - Immunology 2021Quote: ... Bone marrow was collected and red blood cells were lysed with ACK lysing buffer (Lonza, LZ10-548E). Cells were passed through a cell strainer (70 μm pore size ...
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bioRxiv - Immunology 2021Quote: ... Nucleofector Kit V was from Lonza. The original vector pCasper-GR (#FP971 ...
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bioRxiv - Microbiology 2021Quote: ... (DMEM; Lonza) supplemented with 5% fetal bovine serum (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... The Netherlands and Gentamicin sulfate was bought from Lonza BioWhittaker ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T-cells were stimulated with plate-bound α-CD3 (3.75 μg/ml; Immunotech) and soluble α-CD28 (1 μg/mL; Immunotech) in X-vivo 20 serum-free medium (Lonza). X-vivo 20 medium was supplemented with L-glutamine (2 mM ...
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bioRxiv - Biophysics 2021Quote: ... All cell lines were regularly tested for mycoplasma infection and were found to be negative (MycoAlert Plus Detection Kit, Lonza, LT07-710).
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bioRxiv - Immunology 2021Quote: ... the final saponin adjuvant was characterized by Limus Amebocyte Lystae assay (QCL-1000, Lonza) for low endotoxin levels ...
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bioRxiv - Immunology 2021Quote: ... The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza, Walkersville, MD, USA), in the absence or presence of 100 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... Mononuclear cells were isolated with Ficoll-hypaque (γ = 1.077, GE Health) and red blood cells were lysed using ammonium-chloride-potassium (ACK) lysis buffer (Lonza, MD, USA). The remaining mononuclear cells were seeded in 150 x15 mm style non-tissue culture-treated petri dishes or non-tissue culture-treated 24-well plates at 1 ×106 cells/mL ...
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bioRxiv - Immunology 2021Quote: ... HUVEC cells were cultured on a 1% gelatin coated surface in EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM (Lonza) following the manufacturer’s instructions with an additional 6% FBS ...
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bioRxiv - Immunology 2021Quote: Primary normal human bronchial epithelial cells (NHBECs) were obtained from a 65 year old Caucasian male without identifiers sourced from Lonza (NHBE CC-2540; Walkersville, MD). The NHBECs were revived from cryopreservation and expanded with PneumaCult™ Ex-Plus media (StemCell #05040 ...
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bioRxiv - Immunology 2021Quote: ... The enriched CD4+CD25hi cells were cultured in 24-well non-tissue culture plates at 1 x 106 cells/mL in X-Vivo-15 (Lonza) supplemented with 10% heat-inactivated human AB serum (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Splenocyte suspensions were washed in PBS and incubated in ACK Lysis Buffer (LONZA, 0000879569) for 5 minutes at room temperature (RT ...
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bioRxiv - Immunology 2021Quote: ... and in DMEM (Lonza) when HEK-293T cells were used ...
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bioRxiv - Immunology 2021Quote: ... NIH AIDS Research and Reference Reagent Program) were grown at 37°C in a humidified atmosphere with 5% CO2 in RPMI 1640 medium (Lonza, Verviers, Belgium) in the case of the CEM.NKR-CCR5 cells ...
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bioRxiv - Immunology 2021Quote: ... Primary bronchial AECs from adults were purchased from Lonza® or obtained from a tracheal segment lung transplant donor lung tissue ...
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bioRxiv - Immunology 2021Quote: YTS-2DL1 or 721.221/K562 cells were transfected with Nucleofector 2b (Lonza) using Amaxa solution R and protocol X-001 ...
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bioRxiv - Immunology 2021Quote: ... antibodies in serum-free X-Vivo 20 medium (Lonza), in the absence (Th0 ...
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bioRxiv - Immunology 2021Quote: ... and transfected with siGenome SMARTpool small interference RNA (siRNA) oligonucleotides (Dharmacon) using the nucleofection technique by Lonza. Scrambled non-targeting siRNA (5’-AAUUCUCCGAACGUGUCACGU-3’ ...
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (500 ng/mL) at a density of 2 × 106 cells/mL of X-vivo 15 serum-free medium (Lonza). For iTreg differentiation ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% heat-inactivated FCS (Lonza) and 1% 0.5M EDTA (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged for 5 min at 1200 rpm at 20°C and resuspended at 2.5×106 cells/ml in X-VIVO 15 cell culture medium (Lonza) with 50 U/ml penicillin–streptomycin ...
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bioRxiv - Immunology 2021Quote: ... were cultured using endothelial growth media (EGM-2; Lonza). To passage cells ...
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bioRxiv - Immunology 2021Quote: Pooled human umbilical cord vein endothelial cells (HUVECs, Lonza, Basal, Switzerland) were cultured using endothelial growth media (EGM-2 ...
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bioRxiv - Immunology 2021Quote: A single-cell suspension was prepared from spleen and lymph nodes and red blood cells were removed by lysis with ACK buffer (Lonza). CD4+ T cells were enriched using microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2 in X-VIVO 15 (Lonza) supplemented with 5% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin (penicillin 50 U/ml and streptomycin 50 μg/ml, final concentration; Lonza) at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Staining with primary and secondary antibodies was performed in 50 μl PBS (Lonza) containing 0.5% bovine serum albumin and 0.1% sodium azide (PBA) ...
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bioRxiv - Immunology 2021Quote: ... hECs were grown in endothelial cell growth medium (EGM-2 Bulletkit; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2021Quote: ... cells were overlaid with 1:1 mixture of 2% agarose (Lonza, Basel, Switzerland) and 2X MEM medium ...
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bioRxiv - Microbiology 2021Quote: Primary human umbilical endothelial cells (HUVECs) were purchased from Lonza (pooled donors, # C2519A) and cultured in EGM-2 complete medium (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... (DMEM; Lonza) supplemented with 5% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2021Quote: ... high glucose supplemented with 10% fetal bovine serum (FBS) (Lonza, Basel, Switzerland), penicillin (100 U/mL) ...
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bioRxiv - Microbiology 2021Quote: ... The human lung epithelial cell line Calu-3 (ATCC HTB-55) was maintained in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: HepG2 hepatoma cells were cultured in collagen-coated T75 flasks in minimum essential medium eagle with Earle’s BSS (MEM Eagle EBSS) from Lonza (Walkersville, MD) supplemented with 10% FBS and 4.4 mM sodium pyruvate at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Human Bladder Microvascular Endothelial cells (HMVEC-Bd) (procured from Lonza, CC-7016) were cultured in Lonza EGM-2 MV BulletKit medium ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell culture media and buffers were purchased from Lonza and Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... or nucleofection (Lonza #VPH-1001) using 1 µg of a construct encoding Cas9 ...
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bioRxiv - Molecular Biology 2021Quote: ... The nucleofection was performed using the 4D-Nucleofector™ X Unit (Lonza, Germany) with the CB-150 pulse according to manufacturer’s instructions ...