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Citations for Lonza :
551 - 600 of 9698 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... YS-EBs were plated onto 10cm dishes containing X-VIVO 15 medium (Lonza) supplemented with interleukin-3 (IL-3 ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspended in P3 buffer (Lonza). RNP were prepared by mixing Cas9 (Alt-R™ S.p ...
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bioRxiv - Neuroscience 2024Quote: ... the YS-EBs were plated on dishes with X-VIVO 15 medium (Lonza) supplemented with interleukin-3 (IL-3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were validated by sequencing to contain KRAS and TP53 mutations and verified as Mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza, LT07-701). All cells were maintained in monolayer culture at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... The Amaxa Mouse Macrophage Nucleofector Kit was from Lonza (Cologne, Germany). Gene-specific TaqMan primer-probe mixes used for quantitative real-time polymerase chain reaction (PCR ...
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bioRxiv - Molecular Biology 2024Quote: The agarose solution was prepared by dissolving 2.5 g of Seakem LE agarose (Lonza) in 225 ml of Milli-Q water in a microwave ...
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bioRxiv - Neuroscience 2024Quote: ... iNeurons in P3 buffer were then mixed with Cas9 RNP and nucleofected using pulse program CV-110 in the 4D-Nucleofector (Lonza). After nucleofection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... All lines tested negative for mycoplasma using Lonza MycoAlert Plus kit (Lonza, cat # LT07-710).
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bioRxiv - Genomics 2024Quote: ... as well as routinely tested for mycoplasma using the MycoAlert Detection Kit (Lonza, #75866-212). GL261 cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bronchial epithelial basal medium (BEBM) and supplements were purchased from Lonza (Walkersville, MD). Collagen (30 mg/mL) ...
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bioRxiv - Physiology 2024Quote: The gRNA plasmid (1µg) and donor template plasmid (3µg) were introduced into 3T3-L1 cells (1X 10 6 cell) by electroporation (LONZA, Basel, Switzerland). After 48h of electroporation ...
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bioRxiv - Bioengineering 2024Quote: ... Cell pellets were resuspended in P3 buffer with 22% supplement (Lonza, Basel, Switzerland), and combined with the RNP+dsDNA mixture ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were added to the 24 μL reaction cuvettes (Lonza, Basel, Switzerland) and were nucleofected using the EH-100 program (Lonza Amaxa 4D Nucleofector ...
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bioRxiv - Bioengineering 2024Quote: ... 12 µg/mL Bovine Brain Extract (Lonza, CC-4098), 10 ng/mL hEGF (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Primary human umbilical vein endothelial cells (Lonza) and aortic smooth muscle cells (Cell Systems ...
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bioRxiv - Bioengineering 2024Quote: ... were cultured in endothelial growth medium (EGM-2, Lonza) and VascuLife smooth muscle cell medium (LifeLine Cell Technology ...
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bioRxiv - Bioengineering 2024Quote: ... Human microvascular endothelial cells (hMVEC) were purchased from Lonza (hMVEC 33226, Walkersville, MD) and used without additional characterization ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were expanded in EGM-2 MV media (EBM-2 supplemented with Lonza’s SingleQuot supplements ...
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bioRxiv - Developmental Biology 2024Quote: ... 1.5 x 106 cells were transfected with 1 µg DNA (500 ng Cas9 plasmid and 500 ng linearized donor plasmid) by nucleofection (pulse code CA137) using P3 Primary Cell 4D-Nucleofector X kit (Lonza, V4XP-3024) in a 4D-Nucleofector (Lonza ...
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bioRxiv - Developmental Biology 2024Quote: ... in a 4D-Nucleofector (Lonza, AAF-1003B) and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... T cell pellets were resuspended in 20 uL per editing condition in P3 Buffer (Lonza) and then mixed with prepared RNP and DNA HDRT templates ...
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bioRxiv - Bioengineering 2024Quote: ... and activated using anti-human CD3/CD28 dynabeads (Cell Therapy Systems, Thermo) at a 1:1 ratio in XVivo 15 media (Lonza) supplemented with 5% FBS (MilliporeSigma ...
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bioRxiv - Cell Biology 2024Quote: ... using Nucleofector II (Lonza) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... B cells were edited 3-5 days after isolation or thawing using the Lonza Nucleofector 4D (program EO-117) using 1×106 cells per well of a 16-well Nucleocuvette Strip (Lonza). Immediately following nucleofection ...
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bioRxiv - Biochemistry 2024Quote: Coronary artery smooth muscle cells (CASMCs, CC-2583; Lonza; Allendale, NJ) were maintained and cultured in smooth muscle basal media (SmGM-2 BulletKit ...
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bioRxiv - Biochemistry 2024Quote: ... were maintained and cultured in smooth muscle basal media (SmGM-2 BulletKit; CC-3182; Lonza). The media was supplemented with hEGF ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were maintained in SmGM-2 media (Lonza) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... 2.5×105-1×106 CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland, cat.: V4XP-3032) with complexed RNPs and electroporated using the Lonza 4D Nucleofector and 4D-Nucleofector X Unit (program DZ-100) ...
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bioRxiv - Bioengineering 2024Quote: Primary human ventricular cardiac fibroblasts (NHCF-V; Lonza, cat. no. CC-2904) were cultured according to the manufacturer’s protocol with Fibroblast Growth Medium 3 (PromoCell ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in 100 µL nucleofection buffer containing RNP and electroporated according to manufacturer’s instructions using the 4D-Nucleofector (Lonza). Control cells were electroporated with SpCas9 nuclease only.
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bioRxiv - Bioengineering 2024Quote: ... purchased from Lonza Walkersville Inc ...
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bioRxiv - Physiology 2024Quote: ... of six genetically independent donors were grown as monolayers in 100% humidity and 5% CO2 at 37 1C in serum-free defined growth media (BEGM, Lonza). NHBEs (passage 3 ...
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bioRxiv - Physiology 2024Quote: Primary NHBEs (Lonza, Walkersville, MD) of six genetically independent donors were grown as monolayers in 100% humidity and 5% CO2 at 37 1C in serum-free defined growth media (BEGM ...
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bioRxiv - Synthetic Biology 2024Quote: ... using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Physiology 2024Quote: ... while ductal cells were cultured in KGM™ Gold Keratinocyte Growth Medium BulletKit (00192060, Lonza). Cells were harvested by treating 0.05 % Trypsin/EDTA (25300062 ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated by electrophoresis (ProSieve EX Running buffer 10x, diluted in H2O to 1x, (Lonza, Basel, CH); Precision Plus Protein WesternC Standards ...
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bioRxiv - Physiology 2024Quote: ... Endothelial cells were grown in endothelial cell basal medium (EBM-2) supplemented with an endothelial cell bullet kit (EGM-2) (Lonza) and used between passage 3 and 5 in tissue culture dishes coated with 0.1% gelatin and maintained at 37°C in a humidified incubator at 5% CO2 ...
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bioRxiv - Physiology 2024Quote: ... and adipored (0.3 µL per mL, Lonza) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... All cells were screened for mycoplasma contamination with a MycoAlert mycoplasma detection kit (Lonza, Basel, Switzerland) prior to use.
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bioRxiv - Immunology 2024Quote: ... and primary human monocytes and placed in nucleofection cuvettes subjected to program Y-010 for the Nucleofector 2b Device (Lonza). 500µL RPMI medium was immediately added into cuvettes after nucleofections ...
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bioRxiv - Immunology 2024Quote: ... siRNA against human genes or control siRNA were incubated with a mixture of nucleofection solution (Human Monocytes Nucleofector kit; Lonza) and primary human monocytes and placed in nucleofection cuvettes subjected to program Y-010 for the Nucleofector 2b Device (Lonza) ...
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bioRxiv - Genomics 2024Quote: ... The iPSCs were passaged using Versene (Lonza) from one well into three wells of a 6-well plate ...
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bioRxiv - Immunology 2024Quote: ... Non-diseased non-parenchymal cells (NPCs) were purchased from Triangle Research Laboratories (Lonza, Triangle research park, NC). All patients provided written and informed consent and the study was approved by the institutional review board at the University of Colorado—Anschutz.
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bioRxiv - Immunology 2024Quote: ... Keratinocytes were lifted off the plate using Trypsin/EDTA (Lonza, Cat: LONCC-5012) for 5min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Trypsinization was stopped using trypsin neutralizing solution (Lonza, Cat: LONCC-5002) and PBS ...
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bioRxiv - Biophysics 2024Quote: Joklik’s minimal essential medium was purchased from Lonza (Walkersville, MD). Fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then resuspended in 17.6 uL of P3 buffer (Lonza) and transferred to PCR tubes containing the RNP:DNA mixtures ...
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bioRxiv - Bioengineering 2024Quote: ... RNP’s and DNA were electroporated (EP) into T-cells in 16 well EP cuvettes on a 4-D Nucleofector X unit (Cat # V4XP-3032, Lonza, Walkersville, VA) using pulse code EH-115 ...
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bioRxiv - Bioengineering 2024Quote: ... Human primary bone marrow MSCs derived by plastic adherence of mononucleated cells from human bone marrow aspirate donors (Lonza) and were cultured in α-minimal essential medium (αMEM ...
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bioRxiv - Bioengineering 2024Quote: ... Human umbilical vein endothelial cells (HUVEC; #CC-2519, Lonza) were cultured using Endothelial Cell Growth Basal Medium-2 (EBM-2 ...