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Citations for Lonza :
501 - 550 of 9698 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The fixed cells were washed three times with the same buffer and then embedded in 2 % low-melting-temperature agarose (SeaPlaque, Lonza). The embedded samples were post-fixed with 4 % potassium permanganate at 4 °C for 2 h ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in P3 4D-nucleofection buffer (Lonza). TrueCut Cas9 Protein v2 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were routinely checked for mycoplasma contamination using MycoAlert Plus detection kit (Lonza). HEK293T cells were grown in DMEM high-glucose medium (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... serum-starved RPE1 cells where trypsinized and 0.5×106 of the cells were resuspended in 20 μl of the P3 Primary Cell 4D-Nucleofector Kit buffer (Lonza). The cell suspension was then nucleoporated with reporter plasmids with DNA lesions and undamaged control plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... using the 4D-Nucleofector unit (Lonza) with the EA-104 program ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs; Lonza) were cultured in 15 mg bovine hypothalamus extract (#02-102 ...
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bioRxiv - Cell Biology 2024Quote: The 96-wells RAFT system (Lonza) was chosen for 3D cultures ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells (RBCs) were lysed using ACK lysis buffer (Lonza). Cells were then washed and resuspended in Maxpar PBS for cell counting using Trypan Blue (Thermo Fisher).
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bioRxiv - Cancer Biology 2024Quote: ... RBCs were lysed using ACK lysis buffer (Lonza) and washed again with FACS buffer.
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bioRxiv - Cancer Biology 2024Quote: ... Nucleofection was performed using CM-137 program on 4D-Nucleofector™ (Lonza). Cells were carefully resuspended with pre-warmed media ...
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bioRxiv - Cancer Biology 2024Quote: ... Immediately after surgery adipose tissue was place into Medium 199 (M199) (Lonza) with penicillin (100 U/l) ...
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bioRxiv - Cancer Biology 2024Quote: ... Early passage cell lines were tested for Mycoplasma (Lonza #LT07-218) and then were frozen using Bambanker’s freezing media (Bulldog Bio) ...
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bioRxiv - Cancer Biology 2024Quote: ... mycoplasma free (Lonza #LT07-318, #LT07-518 (control) melanoma cell lines were seeded in 6 well tissue cultured dishes in RPMI 1640 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... monocytes were cultured in 24-well plates (Labclinics, Barcelona, Spain) at a concentration of 106 cells/ml in either X-VIVO 15 media (Lonza, Basel, Switzerland) or RPMI 1640 media (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were confirmed to be mycoplasma negative every 3 weeks (MycoAlert™ Mycoplasma Detection Kit, Lonza, Bend, OR, USA). Fifteen micrograms of total whole cell lysates (WCL ...
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bioRxiv - Cell Biology 2024Quote: ... using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cell Biology 2024Quote: ... BEGM growth media (Lonza) was used for proliferation until cells were confluent ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 20 µL of SF Cell Line Nucleofector™ Solution with the supplement added (Lonza V4XC-2032). The cells and the RNP complex were carefully mixed and transferred to a well of the 16-well Nucleocuvette™ ...
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bioRxiv - Cell Biology 2024Quote: ... the human cardiac microvascular endothelial cells (hCMECs, cat. #6000) are isolated from human fetal heart and the human Hepatic Sinusoidal (hLSECs, Lonza #HLECP1) from liver ...
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bioRxiv - Systems Biology 2024Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and placed in a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC-5002); this was repeated to maximize cell collection ...
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bioRxiv - Systems Biology 2024Quote: ... and adherent cells were expanded with bronchial epithelial cell growth medium (Lonza, CC-3170) and then transferred to a collagen-coated transwell plate (Corning ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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bioRxiv - Systems Biology 2024Quote: ... and then dissociated with 0.025% Trypsin/EDTA (Lonza, CC-5012) for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and delivered to parental K562 cells by Amaxa nucleofection using the Amaxa Cell line nucleofector kit V (Lonza VCA-1003). GFP+ transfected cells were single-cell sorted into 96-well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... All of the cell lines were confirmed by single-nucleotide-polymorphism array and short-tandem-repeat authentication (Genetica DNA Laboratories) and routinely tested negative for mycoplasma (MycoAlert, Lonza)
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bioRxiv - Synthetic Biology 2024Quote: ... Isolated T cells were cultured at an initial density of 106 cells/mL in X-Vivo 15 medium (Lonza) supplemented with human serum (5% ...
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bioRxiv - Neuroscience 2024Quote: ... or electroporation with an Amaxa Nucleofector 2b device (AAB-1001, Lonza), all following manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Absence of mycoplasma was assessed every month using the MycoAlert mycoplasma detection kit (LT07-318, Lonza). HEK293 cells were transfected by electroporation with plasmids coding for either Snap-tagged human mGlu2 receptors (STmGluR2 ...
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bioRxiv - Systems Biology 2024Quote: The reporter cell line for the PTGR screen was created by nucleofection of haploid AN3-12 mESCs with 500ng of the reporter construct and 10 µg of a Tol2 transposase encoding plasmid using the Mouse ES Cell Nucleofector Kit (Lonza) according to the manufacturer’s protocol using an Amaxa Nucleofector (Lonza) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 x 105 to 1 x 106 cells were resuspended in 20µL of nucleofection solution (P3 Primary Cell 4D-Nucleofector™; Lonza) and nucleofected (Lonza 4D nucleofector TM Core + X Unit ...
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bioRxiv - Neuroscience 2024Quote: ... and nucleofected (Lonza 4D nucleofector TM Core + X Unit ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC lines were validated by Array Comparative Genomic Hybridization assessment of karyotype and copy number variation (Cell Line Genetics) and confirmed to be sterile and pluripotent via MycoAlert testing (Lonza), Pluritest Array Analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... negative selection of T cells was performed using the EasySep human T cell isolation kit (Stemcell) and cultured in X-VIVO 15 medium (Lonza) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Synthetic Biology 2024Quote: ... resuspended in 20 uL of P3 nucleofection buffer (Lonza) containing 1.2 uL of Cas9 RNP mix and 0.4 ug double-stranded DNA HDR template and electroporated using the EH-115 program ...
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bioRxiv - Cancer Biology 2024Quote: CRISPR-mediated mutagenesis was carried out by transiently introducing CRISPR/Cas9 RNA-nucleoprotein complex into cultured NB4 cells with the 4D-Nucleofector system (Lonza). The sample preparation kit was Lonza SF Cell Line 4D-Nucleofector Kit S (Cat# V4XC-2024) ...
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bioRxiv - Neuroscience 2024Quote: ... and mixed with CRISPR/Cas9 RNPs before nucleofection with CM-137 pulse code in a 96-well nucleofection plate using a 4D-Nucleofector 96-well Shuttle nucleofector (Lonza). After nucleofection ...
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bioRxiv - Neuroscience 2024Quote: ... Four wells of EBs per line were seeded in a 4-layer cell discs coated with growth factor-reduced matrigel in X-VIVO15 medium (Lonza) supplied with GlutaMAX (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... 50’000 preMacs per reaction were resuspended in 20 µl P3 Primary Cell Nucleofector Solution (Lonza) and mixed with CRISPR/Cas9 RNPs before nucleofection with CM-137 pulse code in a 96-well nucleofection plate using a 4D-Nucleofector 96-well Shuttle nucleofector (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... Immortalized PreAdip ( Lonza, Basel, Switzerland) and cultured in the appropriate media (SKBM-2 Bullet kit ...
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bioRxiv - Immunology 2024Quote: ... T cells were cultured in T cell medium containing X-VIVO™ 15 media (Lonza, Basel, Switzerland), 10% human AB serum (Corning Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL of cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Cell Biology 2024Quote: ... and containing MycoZap plus (VZA-2012; Lonza) at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were nucleofected with the 4-D Nucleofector System (Lonza, Cat#AAF-1003X) using the CA-137 program ...
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bioRxiv - Molecular Biology 2024Quote: ... were maintained in EGM™-2 (Endothelial Cell Growth Medium-2 BulletKit™; Lonza) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in full EGM2 medium (Lonza). Media was replaced every two days ...
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bioRxiv - Molecular Biology 2024Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10µM BrdU ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in EBM™-2 (Endothelial Cell Growth Basal Medium-2, Lonza). 500 μl EGM-2 BulletKit™ culture medium (with full supplements ...
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bioRxiv - Molecular Biology 2024Quote: Pooled human umbilical vein endothelial cells (HUVECs; Lonza) were maintained in EGM™-2 (Endothelial Cell Growth Medium-2 BulletKit™ ...
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bioRxiv - Molecular Biology 2024Quote: Normal human bronchial epithelial cells (Lonza, Epithelix) were differentiated in transwell plates at an air-liquid interface ...
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bioRxiv - Molecular Biology 2024Quote: ... the cell pellet was re-suspended in antibody medium (EGM2 CC-3162, Lonza, Basel, Switzerland) with anti-mouse CD31 PE antibody (1:400 ...