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Citations for Active Motif :
601 - 650 of 684 citations for SpectraDye Antibody Labeling Kit 350 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) from four HLHS patients were fixed in 4% paraformaldehyde and stained with antibody against 5-methylcytosine (Active Motif, RRID: AB_2687950)31 ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin immunoprecipitation was performed using the ChIP-IT Express Enzymatic kit according to the manufacturer’s directions (Active Motif). DNA was enzymatically sheared for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionation of MDA-MB-231 and 4T1 cells was first performed using the Nuclear Extract kit (Active Motif). The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif ...
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bioRxiv - Cell Biology 2020Quote: Purified DNA fragments for ChIP-seq were prepared by using the ChIP-IT High Sensitivity Kit (Active Motif) and the related antibodies ...
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bioRxiv - Genomics 2019Quote: ... The libraries were generated with the Illumina Nextera DNA Library Prep kit and were sequenced by Active Motif and NIEHS Sequencing core by Illumina HiSeq 2000 and NextSeq 500 systems with 42bp paired-end reads on each sample ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-seq was performed as described previously (Buenrostro et al., 2015) using an ATAC-seq kit (Active Motif) as described by the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... histones were extracted according to the recommended manufacturer’s one-step protocol using the Histone Extraction Kit (Active Motif). The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were Polytron minced and homogenized by douncing in Prep Buffer (ChIP-IT High Sensitivity Kit, Active Motif). They were then sonicated in 100 µl of sonication buffer (0.1%SDS ...
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bioRxiv - Cell Biology 2021Quote: Cytoplasm and nucleus of keratinocytes were separated by using Nuclear Extract Kit (Active Motif, Inc., Carlsbad, CA, USA) following the manufacturer’s instructions ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: NRF2-ARE binding assay was performed with an ELISA-based TransAM NRF2 Assay Kit (Active Motif, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Chromatin immunoprecipitation (ChIP) assays were conducted using the ChIP-IT EXPRESS assay kit protocol (Active Motif, Carlsbad, CA). After exposure to Aβo ...
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bioRxiv - Neuroscience 2024Quote: Nrf2 activity was quantified using nuclear extracts and a TransAm NRF2 activity kit from Active Motif (cat# 50296) following the manufacturers included instructions.
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bioRxiv - Immunology 2023Quote: ... Chromatin immunoprecipitation (ChIP) was carried out using the ChIP-IT Express Enzymatic kit from Active Motif (Rixensart, Belgium). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin for re-ChIP was prepared using the ChIP-IT High Sensitivity Kit (Active Motif, catalog no. 53040), as described above ...
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bioRxiv - Genomics 2024Quote: Human islet ATAC-seq libraries were prepared following the Active motif ATAC prep kit (Active motif catalog# 53150). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets used for fractionation were fractionated using the Nuclear Complex Co-IP kit from Active motif (#54001) according to their protocol.
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Biochemistry 2021Quote: ... rabbit polyclonal anti-Pre6 (gift from D. Wolf, 1:5000) and anti-Rpb1 (RNA Pol II) monoclonal mouse antibody (Active Motif, 4H8, 1:2000). For rabbit primary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Global % 5mC levels were assayed using a Global DNA Methylation LINE-1 Kit (55017; Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Cells from two independent experiments were collected and open chromatin was assessed using an ATACseq kit (Active Motif, 53150). DNAseq library preparation was completed at the Oncogenomics Core Facility at the Sylvester Comprehensive Cancer Center ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif). The samples were run in duplicates and absorbance was measured using a spectrophotometer at 450 nm.
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bioRxiv - Molecular Biology 2021Quote: Chromatin was prepared from flash-frozen liver tissue using the Active Motif ChIP-IT High Sensitivity kit (Active Motif), employing a modified protocol described in (47) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear fractions were extracted following the manufacturer’s instruction supplied with the Universal Magnetic co-IP kit (54002, Active Motif). 1000μg of proteins were immunoprecipitated using 5.0μg of antibody followed by protein G magnetic beads ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... were raised against KLH-conjugated dinucleosides at the Technology Development Laboratory (Babraham Bioscience Technologies Ltd, Cambridge, UK) (anti-mCA antibody clone 2C8H8A6 currently available from Active Motif, Cat. No 61783/4). Alexa Fluor secondary antibodies were purchased from Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... the RevMAb Biosciences (clone RM362, at 1:300 dilution) and the mouse monoclonal recombinant AbFlex m6A antibody (rAb) from Active Motif (at 1:300 dilution). A mixture of reference DNA oligonucleotides that correspond to the position of the modified nucleotides was used to compare with antibody binding (the last base at the 5’ end of the oligonucleotide hybridizes with the modified base ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear extracts were isolated using the reagents and protocol provided in the Active Motif Nuclear Extract Kit (Active Motif). IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif) ...
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bioRxiv - Developmental Biology 2019Quote: ... All H3K27ac ChIP experiments were processed as ChIP-seqs using the reagents from ChIP-IT High Sensitive kit (Active Motif). IPs were performed in parallel technical duplicates with 11 to 14µg of chromatin on each ...
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bioRxiv - Microbiology 2019Quote: ... Cell fixation was performed using the fixation reagents and steps from the Low Cell Chip Kit (Active Motif; Cat#53084) as described in the user manual ...
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bioRxiv - Microbiology 2019Quote: Immunoprecipitation of both RNA-DNA hybrids (DRIP) and γH2A was performed using the ChIP-IT Enzymatic Express kit (Active Motif) with formaldehyde fixed chromatin samples ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays at the HES1 promoter were performed in HT29 cells using the CHIP-IT kit (Active Motif, Carlsbad, USA). Sonicated chromatin was immunoprecipitated with antibodies against IgG (sc-3739 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Cancer Biology 2021Quote: ... The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... After quenching with 0.5M glycine the HMGN-nucleosome complexes were immunoprecipitated using ChIP-IT Express kit (Active Motif, Cat. No. 53008) with either anti-HMGN1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified DNA was analyzed by ChIP-qPCR data relative to input using the mouse negative control and mouse positive control primer set Gapdh in the ChIP-IT qPCR analysis kit (Active Motif). Reaction conditions were programmed with initial denaturation at 50°C for 2 minutes and 95°C for 10 minutes followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tagmentation Master Mix was added to the pellet and reaction was incubated in a themomixer at 600-800 rpm set at 37C for 30 min as indicated by manufacturer (ATAC-Seq kit, 53150, Active Motif).