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Citations for Active Motif :
601 - 650 of 713 citations for SpectraDye Antibody Labeling Kit 350 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Cell Biology 2024Quote: ... For assessing histone modification 70 μg of chromatin from WT and KO cells was taken and 3ug antibodies were added H3K27ac (Active motif, #39134), H3K27me3 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: The amount of GTP-bound RAS was determined using the Ras GTPase Chemi ELISA Kit (Active Motif North America ...
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bioRxiv - Molecular Biology 2021Quote: Nuclear proteins were prepared using fibroblasts at 80-90% confluency and the Nuclear Extract Kit (Active Motif), according to the manufacturer’s protocol.
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bioRxiv - Genomics 2019Quote: Extraction of nuclear proteins and coimmunopreciptation were performed using the Universal Magnetic Co-IP Kit (Active Motif). Briefly ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by 3 minutes incubation with Stop Solution from the ChIP-IT High sensitive kit (Active Motif). Samples were then washed 3 times with working Washing Solution (ChIP-IT ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin immunoprecipitation (ChIP) was carried out using the ChIP-IT Express Enzymatic Chromatin Immunoprecipitation Kit (Active Motif) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 4T1 and 6DT1 cells using the ChIP-IT Express Enzymatic kit (Active Motif) according to manufacturers’s instructions as mentioned above ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
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bioRxiv - Neuroscience 2022Quote: CUT&Tag was performed from 450k nuclei using the Cut&TagIT Kit following manufacturer’s protocol (Active Motif).
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bioRxiv - Immunology 2019Quote: ... Subsequent chromatin preparation steps were performed according to the “High Sensitivity Chromatin Preparation” Kit (Active Motif, 53046). Samples were sonicated two times for 10 minutes (30 seconds on ...
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bioRxiv - Developmental Biology 2021Quote: ChIP experiments were carried out using the ChIP-IT Express Enzymatic kit (Cat. No. 53009, Active Motif) or the True MicroChIP Kit (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed and the beads washed with 665µL of ChIP Buffer (from Active Motif kit) by inverting the tube several times ...
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bioRxiv - Molecular Biology 2021Quote: ATAC-seq was performed in biological duplicate using the ATAC-Seq Kit (Active Motif, catalog No.53150). Briefly ...
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bioRxiv - Genomics 2024Quote: ... and Illumina sequencing libraires were prepared using the Low Cell ChIP-Seq kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP was then performed using a ChIP-IT High Sensitivity kit (#53040, Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: For DNA Chromatin Immunoprecipitation (ChIP) we used the ChIP-IT High Sensitivity® kit from Active motif. Lysates were obtained as described in the RIP protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and nuclei was extracted using ice-cold ATAC-lysis buffer (according to the kit protocol; Active Motif) and the pellet resuspended in the Tn5 tagmentation master mix ...
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bioRxiv - Microbiology 2023Quote: ... Histones were isolated from inhibitor treated or untreated cells using the histone extraction kit (Active Motif, 40028) and western blots performed ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif, 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
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bioRxiv - Cancer Biology 2024Quote: ... The protocol was followed to produce Illumina compatible libraries using the ATAC-seq kit from Active Motif. Briefly ...
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bioRxiv - Molecular Biology 2024Quote: CUT&Tag experiments were performed in duplicates using the CUT&Tag-IT Assay Kit (Active Motif #53160) and following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies and dilutions were used: anti-Esrrb (PP-H6705-00, Perseus Proteomics, 1:1000) and anti-Histone H3 (61476, Active Motif, 1:5000).
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin immunoprecipitation was performed using the ChIP-IT Express Enzymatic kit according to the manufacturer’s directions (Active Motif). DNA was enzymatically sheared for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionation of MDA-MB-231 and 4T1 cells was first performed using the Nuclear Extract kit (Active Motif). The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif ...
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bioRxiv - Cell Biology 2020Quote: Purified DNA fragments for ChIP-seq were prepared by using the ChIP-IT High Sensitivity Kit (Active Motif) and the related antibodies ...
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bioRxiv - Genomics 2019Quote: ... The libraries were generated with the Illumina Nextera DNA Library Prep kit and were sequenced by Active Motif and NIEHS Sequencing core by Illumina HiSeq 2000 and NextSeq 500 systems with 42bp paired-end reads on each sample ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-seq was performed as described previously (Buenrostro et al., 2015) using an ATAC-seq kit (Active Motif) as described by the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... histones were extracted according to the recommended manufacturer’s one-step protocol using the Histone Extraction Kit (Active Motif). The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were Polytron minced and homogenized by douncing in Prep Buffer (ChIP-IT High Sensitivity Kit, Active Motif). They were then sonicated in 100 µl of sonication buffer (0.1%SDS ...
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bioRxiv - Cell Biology 2021Quote: Cytoplasm and nucleus of keratinocytes were separated by using Nuclear Extract Kit (Active Motif, Inc., Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets used for fractionation were fractionated using the Nuclear Complex Co-IP kit from Active motif (#54001) according to their protocol.
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bioRxiv - Genomics 2024Quote: Human islet ATAC-seq libraries were prepared following the Active motif ATAC prep kit (Active motif catalog# 53150). Briefly ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: NRF2-ARE binding assay was performed with an ELISA-based TransAM NRF2 Assay Kit (Active Motif, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Chromatin immunoprecipitation (ChIP) assays were conducted using the ChIP-IT EXPRESS assay kit protocol (Active Motif, Carlsbad, CA). After exposure to Aβo ...
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bioRxiv - Neuroscience 2024Quote: Nrf2 activity was quantified using nuclear extracts and a TransAm NRF2 activity kit from Active Motif (cat# 50296) following the manufacturers included instructions.
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bioRxiv - Immunology 2023Quote: ... Chromatin immunoprecipitation (ChIP) was carried out using the ChIP-IT Express Enzymatic kit from Active Motif (Rixensart, Belgium). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin for re-ChIP was prepared using the ChIP-IT High Sensitivity Kit (Active Motif, catalog no. 53040), as described above ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Biochemistry 2021Quote: ... rabbit polyclonal anti-Pre6 (gift from D. Wolf, 1:5000) and anti-Rpb1 (RNA Pol II) monoclonal mouse antibody (Active Motif, 4H8, 1:2000). For rabbit primary antibodies ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight with Concanavalin A beads and 1 µl of the primary anti-H3K4me1 antibody per reaction (Active Motif, catalog number 39297) and 1 µl of the primary anti-H3K27ac antibody per reaction (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... Global % 5mC levels were assayed using a Global DNA Methylation LINE-1 Kit (55017; Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...