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Citations for Active Motif :
501 - 550 of 684 citations for SpectraDye Antibody Labeling Kit 350 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... This DNA was then used in immunoprecipitation (IP) reactions using 5-Methylcytosine (5-mC) mouse monoclonal antibody (39649, Active Motif). Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step ...
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bioRxiv - Immunology 2019Quote: ... Protein-DNA complexes were immuno-precipitated using 3μg of H3K27ac antibody from the same lot for all ChIP experiments (Catalogue #39133; Active Motif) coupled to 50μl Protein G Dynabeads (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 µg of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 01518010). Complexes were washed ...
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bioRxiv - Genomics 2021Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
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bioRxiv - Genetics 2020Quote: ... containing primary antibody (rabbit anti-H3K9me3, Abcam ab8898; rabbit anti-H3K27me3, Cell Signaling Technology C36B11; rabbit anti-H3K4me3 Active Motif 39159 ...
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bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
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bioRxiv - Neuroscience 2022Quote: ChIP-seq libraries were constructed from FACS-enriched RGCs (100,000 cells for each library) using an H3K27me3 antibody (1:100, Active Motif) or normal rabbit IgG (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Glucosylation and biotinylation of 5hmC were performed with the Hydroxymethyl Collector kit (Active Motif 55013), followed by on beads-exonuclease digestion of the captured fragments and library preparation (TrueSeq library preparation kit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Gel mobility shift assay was carried out using Gelshift Chemiluminescent EMSA kit (#37341, Active Motif) with modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... the ChIP was performed using ChIP-IT High Sensitivity kit (Active Motif, Carlsbad, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclear protein fractions were prepared by using Nuclear Complex co-IP kit (Active Motif #5400), according to the manufacturer ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... Reciprocal co-immunoprecipitations were performed using active motif Co-IP kit (Active Motif, Carlsbad, CA) in moderate stringency buffer (IP High buffer [Active Motif] and Protease inhibitor cocktail [Sigma] ...
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bioRxiv - Cancer Biology 2022Quote: ChIP-seq was performed using the ChIP-IT High Sensitivity kit (Active Motif, cat. 53040) as described previously (38) ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunoprecipitation and elution were performed using the ChIP-IT High Sensitivity kit (Active Motif #53040) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Nuclear extracts from Jurkat cells were prepared using a Nuclear Extract Kit (40010, Active Motif), according to manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2022Quote: Making libraries and sequencing: Libraries were made as in ATAC-Seq kit (Active Motif, 53150) with some modifications ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear and cytoplasmic RNA separation assay was conducted using RNA Subcellular Isolation Kit (Active motif), according to previously detailed method(61) ...
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bioRxiv - Cancer Biology 2023Quote: ChIP-seq was performed using the ChIP-IT High Sensitivity kit (Active Motif, cat. 53040) as described previously (24) ...
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bioRxiv - Genomics 2023Quote: ... chromatin was extracted from CT26 cells using the Nucleosome Preparation Kit (cat: 53504, Active Motif). The enzymatic digestion time for this cell line was optimized to 30min and the mononucleosome preparation protocol was followed as per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: Re-ChIP was performed using the Re-ChIP-IT Kit (Active Motif, catalog no. 53016) according to the manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ChIP assays were performed using the ChIP-IT Express Enzymatic Kit (Active Motif, Carlsbad, CA) following manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: ... and tagmented using the enzyme and buffer provided in the ATAC-Seq Kit (Active Motif). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP was performed using the ChIP-IT Express Enzymatic Kit (Active Motif, catalog no. 53009), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: CUT&Tag libraries were prepared using the CUT&Tag-IT Assay Kit (Active Motif, 53160) following to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear proteins were extracted using the Nuclear Complex Co-IP Kit (Active Motif, Carlsbad, CA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Co-immunoprecipitation (co-IP) was performed using the Nuclear Complex Co-IP Kit (Active Motif). 6T1 Smarcd1 OE cells were seeded onto 15 cm tissue culture dishes at a seeding density of 5×106 cells per dish ...
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bioRxiv - Cancer Biology 2024Quote: ChIP was performed using the ChIP-IT Express Enzymatic Chromatin Immunoprecipitation Kit from Active Motif, following the provided instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... 1 × 105 BM pDCs sorted from Hdac3fl/fl control or Hdac3fl/fl CreERT2 littermates were bound to beads and were subjected for immunoprecipitation with primary anti-H3K27ac antibody (Active Motif) at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... The following primary antibodies were purchased and used for immunofluorescence and CUT&RUN27,28 followed by sequencing: Rabbit anti-H3K4me3 (Active motif, 39159), Rabbit anti-H3K27me3 (Active Motif ...
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bioRxiv - Developmental Biology 2020Quote: ... ChIP-Seq of T7 and MED12 were done in the presence of Drosophila spike-in chromatin and antibody following the manufacturers’ protocol (Active Motif). ChIP-Seq library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analysed by immunoblotting with anti-DNMT1 antibody (Active Motif).
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bioRxiv - Cancer Biology 2021Quote: ... and a p300 antibody (Clone NM11, 1:200 for ChIP, 1:1000 for IB, 1:100 for IP) were purchased from Active Motif. The antibody against cMyc-AcK148 was prepared in a previous study 28 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
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bioRxiv - Immunology 2020Quote: ... and then incubated for 2 hours at room temperature with respective primary antibodies (at dilutions of 1:100): H3K27me3 (39155, Active Motif); Ubiquityl-histone H2A Lys119 (8240 ...
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bioRxiv - Genomics 2022Quote: ... 0.25% NP-40) and incubated for 1 h with 10 μg/IP of mouse bridging antibody (Active Motif; 1 μg/μl) at 4°C with end-to-end rotation ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with anti-5mC antibody (1:200 diluted in 0.5% goat serum + PBST, 39649, Active Motif, CA, USA) at 4 °C overnight and then washed three times with PBST for 5 min each ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight with the following antibodies diluted at 1:2,500: anti-H3 (clone 1B1B2) (Cell Signalling Technology, #14269]) anti-H3K9ac (Active Motif, #39137) anti-H3K27ac [Active Motif ...
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bioRxiv - Genomics 2023Quote: ... The membrane was then blocked in 5% skimmed milk in TBS containing 0.1% Tween 20 (TBST) for 1 h at room temperature followed by incubation with 1:10,000 dilution of 5hmC antibody (Active Motif, 39069) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP assays were performed according to the protocol of ChIP-IT Express Enzymatic kit (Active Motif) using ChIP-grade antibody against V5 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: The ChIP experiments were performed using the ChIP-IT-Express kit (Active Motif catalog no. 53009). Cells were fixed and cross-linked using 1% formaldehyde at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Harvest and isolation of nuclear and cytoplasmic fractions were performed using Nuclear Extract Kit (Active Motif). Samples were quantified and immunoblotted as described above ...
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bioRxiv - Biochemistry 2019Quote: Nuclei were purified using the Active Motif Nuclear Complex Co-IP Kit (Active Motif, Carlsbad, CA) as described in the manufacturer’s manual (www.activemotif.com/catalog/25/nuclear-complex-co-ip-kit) ...
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2021Quote: ... cells were re-suspended in Glycine Stop-Fix Solution (ChIP-IT Enzymatic Express Kit; Active Motif) and kept shaking for 5 mins at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear fraction digestion and collection was performed using Nuclear Complex Co-IP Kit (Active Motif, 54001). 25µl per IP of GFP-Trap Magnetic Agarose beads (Chromotek ...