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Citations for Active Motif :
451 - 500 of 684 citations for SpectraDye Antibody Labeling Kit 350 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The following primary antibodies were used: anti-FOXA1 (GeneTex, catalog no. GTX100308 and Active motif, catalog no. 39837) and GATA3 (Assay Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... and FAC-sorted β-cells were used for histone H3K27ac ChIPseq with anti-H3K27ac antibody (Active Motif, 39133). ChIP and ChIPseq were performed as previously described (Kuo et al. ...
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bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
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bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μg of spikein antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then incubated overnight at 4C with the primary antibodies in PBST (Active motif (2AG-6F12-H4) anti-H3K9me3 (1:100) ...
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bioRxiv - Genomics 2023Quote: ... incubated for 45 min at RT with primary antibody diluted in 1X PBS/1% BSA (H3K27me3, Active motif-61017 ...
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bioRxiv - Genomics 2024Quote: ... Soluble chromatin fractions were incubated with antibodies (anti-H3K9me2, Abcam ab1220; anti-H3K9me3, Active Motif, catalog no 39161) overnight at 4°C ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... 1 mg of total HEK293T cellular extract was incubated with 5 μg of anti-SET8 antibody (Active Motif, 61009), anti-LSF antibody (Millipore,17-10252) ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against Histone H3K27me3 (clone: 39155, Active Motif) Complexes were washed ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3.3K27M antibody (1:500, 61803, Active Motif) diluted in a blocking solution was added and incubated for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... the bead/nuclei mixture was incubated overnight with a 1:100 dilution of primary antibody (H3K27ac (39133, Active Motif)) which was followed by secondary antibody (13-1047 ...
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bioRxiv - Genetics 2023Quote: ... The collected supernatants were incubated overnight with Protein A dynabeads (Life technology, 10001D) associated with H3K27Ac antibody (Active Motif). Next the input sample and chromatin-beads complexes sample were digested ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear or cytosolic fractions were prepared using a Nuclear Extract Kit (Active Motif, USA).
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: Chromatin immunoprecipitation was carried out using a ChIP-IT Express Enzymatic Kit (Active Motif). Tissues were quickly thawed and 200 mg of each was weighed ...
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bioRxiv - Immunology 2019Quote: Chromatin Immunoprecipitations were performed following manufacturer’s instructions (ChIP-IT Express Shearing Kit, Active Motif). In brief ...
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bioRxiv - Genetics 2019Quote: ... proteinase K and DNA purified using the ChIP DNA Purification kit (Active Motif #58002) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear and cytoplasmic protein lysates were prepared using the Nuclear Extract Kit (Active Motif) or Nuclear Complex Co-IP Kit (Active Motif) ...
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bioRxiv - Cancer Biology 2020Quote: Nuclear and cytoplasmic fractionation was performed with the Nuclear Extract Kit (Active Motif, 40010). Cells collected from 15 cm cell culture plates were centrifuged and resuspended in 500 µL 1X Hypotonic buffer and incubated for 5 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIPs were performed using the ChIP-IT High Sensitivity kit (Active Motif, ref. 53040), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and total RNA was isolated using the Qiagen RNeasy Mini Kit (Qiagen, Active Motif). Directional poly-A RNA sequencing libraries were prepared and sequenced as 42-bp paired-end reads on an Illumina NextSeq 500 instrument (Illumina ...
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bioRxiv - Genomics 2021Quote: ... gDNA was treated with bisulfite using the Methyl Detector Bisulfite Modification Kit (Active Motif) and then purified ...
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bioRxiv - Molecular Biology 2021Quote: Chromatin immunoprecipitation (ChIP) experiments were performed using the ChIP-IT Express Kit (Active Motif) as described (106) ...
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bioRxiv - Neuroscience 2021Quote: Chromatin immunoprecipitation was performed using the Low Cell ChIP-Seq kit (#53048, Active Motif) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ChIP was carried out using the ChIP-IT Express kit (Active Motif, cat# 53008). Briefly ...
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bioRxiv - Genomics 2022Quote: ... we used CUT&Tag-IT™ assay kit (Cat. No. 53160) from Active Motif® ...
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bioRxiv - Genetics 2022Quote: ... Samples were tagmented and subsequently purified using an ATAC-seq kit (Active Motif, 53150) according to the cell sample protocol ...
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bioRxiv - Pathology 2023Quote: ... Cells were lysed to ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit (Active Motif 53008). Optimal sonication conditions were determined previously in order to obtain DNA fragments of about 500 bp ...
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bioRxiv - Cell Biology 2023Quote: Accessible genomic DNA was obtained for sequencing using an ATAC-seq kit (Active Motif) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and nuclear fraction was acquired using Nuclear Complex co-IP kit (Active Motif, 54001), which allows a gentle release of DNA-bound protein complexes in the nucleus ...
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bioRxiv - Cell Biology 2023Quote: Cut&Tag was performed using a Complete CUT&Tag-IT Assay Kit (Active Motif) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation was performed using the Nuclear Complex Co-IP kit (Active Motif, USA) according to manufacturer’s protocol until isolation of the nucleus ...
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bioRxiv - Immunology 2024Quote: ... and chromatin isolated using the ChIP-IT Express Enzymatic Shearing Kit (Active Motif, #53009). Chromatin complexes were immunoprecipitated using a rabbit anti-Histone H3 (acetyl K27 ...
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bioRxiv - Cancer Biology 2024Quote: 4T1 nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif).
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bioRxiv - Cancer Biology 2024Quote: ... H3K27ac and H3K27me3 ChIPs were performed using ChIP-IT High Sensitivity kit (Active Motif) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Chromatin immunoprecipitations were performed using ChIP-IT high-sensitivity kits (cat #53040, Active Motif) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitations were performed using 30 μg of cell line chromatin and 6 μg of N-Myc antibody (Active Motif # 61185) or 4 μg of H3K27Ac antibody (Active Motif #39133) ...
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bioRxiv - Genomics 2019Quote: ... and 1x protease inhibitor) before being resuspended in 50 μL of Buffer 2 containing 0.5 μL antibody (H3K27ac from Active Motif, 39122 ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
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bioRxiv - Neuroscience 2022Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S; anti-HDAC2, Active motif, #31505 ...