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Citations for Vector Labs :
2351 - 2388 of 2388 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... at a concentration of 1:200 and a fluorescent-conjugated lectin (WFA) which binds carbohydrate structures and excites at 495 nm (Vector Laboratories, cat. # 1351) at a concentration of 2 ng/mL in blocking solution (2 hr ...
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bioRxiv - Immunology 2024Quote: ... The tissues were counterstained with 1 µg/ml DAPI and slides were mounted in Vectashield Vibrance antifade mounting media (Vector Laboratories, H-1700). Fragments of the lung were fixed at room temperature in 2% PFA in PBS (pH = 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 × 5 min in TBS buffer and then incubated for 100 min in a solution of streptavidin Dylight 649 (Vector laboratories, SA5649, 1:100) in TBS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The histological sections were washed three times (10 min/each) with PBS, counterstained with 4’, 6-diamidino-2-phenylindole (DAPI, 1:16000) and mounted with Vecta-Shield (H1000, Vector Laboratories, Burlingame, CA, USA) (Varman et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 3% BSA with fluorophore-conjugated lectin added 1:1000 (Vector Labs FL-1111-2). Samples were covered and incubated on ice for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the brain sections were re-stained the Nb-loaded neuron using the avidin-biotin-horseradish peroxidase complex (Vectastain Elite ABC; Vector Laboratories, 1:200 dilution) with diaminobenzidine and nickel as demonstrated previously [23,54] ...
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:200 in 1% PBA and 30 minutes with an ABC mix solution from the VECTASTAIN Elite ABC Reagent kit (Vector Laboratories, PK-6101, RRID:AB_2336820). To reveal the reaction ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling slides in a Coplin jar filled with Citrate-Based Antigen Unmasking Solution (1:100 dilution; Vector Laboratories, Cat# H-3300). After cooling to room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Tadpoles are washed with PBT for 1 hour and then washed in PBS for another hour before mounting in Vectashield (Vector Laboratories, Burlingame, CA, USA). Z-stacks were acquired at 1μm for visualizing epidermal MCC’s and 3μm for visualizing entire brain regions.
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bioRxiv - Neuroscience 2022Quote: ... Signal amplification was completed by incubation with streptavidin conjugated with Alexa-Fluor™-488 (1:500 in 0.1% Triton/TBS; Vector Labs, No. SA-5488). Sections were washed in TBS as described above and mounted using aqueous mounting medium containing DAPI (Fluoroshield ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... we treated the brain sections with the Fluorescein (FITC) labeled Wisteria Floribunda Lectin (WFA, WFL) (1:500 dilution in 1X PBS, Vector labs, cat#FL-1351) for 2 hours at RT before mounting them in clear mounting medium (DAKO ...
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bioRxiv - Microbiology 2022Quote: ... Removal of α(2,3)-linked and α(2,6)-linked sialic acids on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 μg/ml; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA ...
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bioRxiv - Neuroscience 2023Quote: ... We incubated free floating sections of the brain in H2O2 (1:50) for 30 minutes then blocked it with 10% Normal Horse Serum (Vector Laboratories, Burlingame, CA, USA) for one hour ...
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bioRxiv - Immunology 2023Quote: ... The brain lysate was 1:10 diluted in the PBS and stained with rhodamine-labeled dolichos biflorus agglutinin (DBA-red, Vector Laboratories, RL-1032-2). GFP and DBA-red double-positive cysts were counted and diluted to 5 cysts per 200 uL of PBS ...
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...
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bioRxiv - Physiology 2023Quote: ... Muscle sections were incubated in 1:1000 Wheat germ agglutinin (WGA– fluorescein 5 μg/mL; Cat# FL-1021-5, Vector Laboratories Ltd., Peterborough, UK) for 10 min followed by three washes of PBS-T for 5 min each ...
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bioRxiv - Neuroscience 2023Quote: ... slices were rinsed several times using 100 mM PBS and subsequently incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed with PBS and incubated for 1 h at room temperature with a protein-blocking solution (VECTASTAIN ABC kit, Vector Laboratories, Burlingame, CA, USA). Excess blocking solution was drained ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase activity was reduced using endogenous peroxidase and alkaline phosphatase blocking solution (Blockxol, cat# SP-6000) followed by blocking for 1 hr at room temperature using animal-free blocker (Vector Laboratories, cat# SP-5035). Then the sections were stained with primary antibody diluted in SignalStain Antibody Diluent (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were then administered a lethal dose of sedative (sodium pentobarbital) followed by an intravenous injection of DyLight 594-conjugated tomato lectin (100 µl; Vector Laboratories, #DL-1177-1). Mice that received stereotactic surgery were only given injections of tomato lectin (no prior anesthesia/administration of dextran) ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were treated with AB solution for 30 min at R/T (Avidin-Biotinylated Horseradish Peroxidase H from Vectastain-ABC KIT of Vector Laboratories 1:200 in PBT).
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bioRxiv - Bioengineering 2022Quote: ... MA) was used as a marker of hypoxia and lectin (1 mg/kg; DyLight 594 labeled lycopersicon Esculentum tomato, Vector Laboratories, DL-1177, Burlingame, CA) as a marker of ischaemia [17] ...
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bioRxiv - Microbiology 2021Quote: ... the filter pieces were embedded in a 4:1 mix of Citifluor (Citifluor Ltd, London, United Kingdom) and Vectashield (Vector Laboratories, Inc., Burlingame, United States), and stored overnight at −20°C for later microscopy evaluation.
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections (40 µm) through the mPFC were sectioned using a sliding microtome and stained with WFA (1:500, Vector Laboratories, FL-1351, RRID AB_2336875) and rabbit anti-PV antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: Single chain HIV-1 gp140 SOSIP variant strain BG505 [52] was purified over agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5). Column was washed with PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was then replaced with imaging 2i medium (FluoroBrite DMEM [Thermo Fisher Scientific, A1896701] containing VectaCell Trolox Antifade Reagent for Live Cell Imaging (Vector Laboratories, CB-1000; 1:1,000). After image acquisition ...
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bioRxiv - Neuroscience 2024Quote: ... as previously described.18 Pan-vascular labeling was achieved through staining with DyLight-594 labeled Lycopersicon Esculentum (Tomato) Lectin (Vector labs, cat. no.: DL-1177-1), which was added to both primary and secondary incubations at 1:100 concentration ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS for 1 h at room temperature and mounted in Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories H-1002-10 and H-1000-10). Images used for analysis were captured using a Nikon E800 fluorescent microscope with a 10x objective ...
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bioRxiv - Systems Biology 2021Quote: ... #415190-9041-000) were treated with UV light for 1 h and coated with APES (3-aminopropyltriethoxysilane) using Vectabond reagent (Vector Laboratories, Burlingame, CA, USA, cat. #SP-1800-7) according to the manufacturer’s protocol ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: Fluorescein-conjugated Sambucus nigra lectin (SNA) that binds sialic acid and Erythrina cristagalli lectin (ECL) that binds galactose were acquired from Vector Laboratories (SA-5001-1 and FL-1141-5, respectively).