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Citations for Vector Labs :
2101 - 2150 of 2232 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... fixed in 3% paraformaldehyde for 20 min and permeabilised for 1 h with 0.1% triton-X-100 prior to blocking in 2.5% normal horse serum (Vector Laboratories, Peterborough, UK) for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then washed in 1X PBS and counterstained with Hoescht 33342 (1:2000 in 1X PBS) for 10 minutes All samples were then mounted in Vectashield (Vector Labs) and imaged on with a Nikon A1R laser scanning confocal microscope with appropriate filter sets and laser lines.
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bioRxiv - Cell Biology 2023Quote: Vascular perfusion was assessed by injecting 10 μl of DyLight-649 conjugated Lycopersicon Esculentum (Tomato) lectin (DL-1178-1, Vector Laboratories) intracardially (1mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at room temperature before the coverslips were washed and finally mounted onto glass slides with Vectashield (Vector Laboratories) mounting solution ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in ABC solution (Peroxidase Standard Vectastain Elite ABC-HRP Kit, Vector Laboratories, Catalog# PK-6100). After 2 x 5min washes in 50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... This was followed by incubation in with biotinylated goat anti-rabbit and donkey anti-guineapig antibodies (Table S5) for 4 h at room temperature then in avidin–biotinylated horseradish peroxidase complex (Elite ABC; 1:300; Vector Laboratories) diluted in PBS for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in biotinylated goat anti-rabbit diluted in TBS-X (1:1,000; BA-1000; Vector Laboratories Ltd., Burlingame, USA) for 2 h at RT followed by incubation in avidin-horseradish peroxidase complex (Vectastain ABC Elite ...
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bioRxiv - Cell Biology 2023Quote: ... and differentiation rate was estimated after 96 h by using Dolichos Biflorus Lectin conjugated to Fluorescein (DLB 1:200, FL-1031; Vector Laboratories) to stain the wall of the cysts in formation and α-Sag1 antibody to stain the tachyzoites ...
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bioRxiv - Neuroscience 2023Quote: ... α-d-galactose carbohydrate residues on sensory neurons was visualized using the Bandeiraea simplicifolia isolectin B4 (IB4) conjugated to rhodamine at a 1:250 concentration (RL-1102; Vector Laboratories). Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004 ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed (0.1M PBS and 0.1M PB) and incubated with Vectastain Elite ABC-HRP Kit (1:200; Vector Laboratories PK-6100). Sections were developed for 5 min in DAB solution (3,3’diaminobenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... Images were analyzed and edited with ImageJ 2.0.0. (NIH). HRP-labelled sections were revealed (approx. 1 min) using the Diaminobenzidine (DAB) Substrate Kit (Vector Laboratories, SK-4100). The enzymatic reaction was stopped with ddH2O and samples were counterstained with Harry’s hematoxylin (15 s ...
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bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
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bioRxiv - Cell Biology 2023Quote: ... Chambers were first blocked with 1% Pluronic F-127 for 30 min and then with a mixture of 1 mg/mL β-casein and 0.1 mg/mL avidin DN (Vector Laboratories) for at least 30 min ...
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bioRxiv - Physiology 2024Quote: ... slides were placed in a blocking buffer (1:60 normal goat serum [Vector Laboratories; Cat. #S-1000] in 0.1% BSA/PBS) for 30 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed 3 x 5 mins in PBST and incubated with fluorescein isothiocyanate-conjugated streptavidin (1:50; Vector Laboratories) in blocking buffer for 30 mins at RT ...
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bioRxiv - Physiology 2024Quote: ... then rinsed several times in PB followed by incubation for two hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit PK6100, Vector Laboratories). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Physiology 2023Quote: ... Then the sections were treated in avidin/biotin-peroxidase complex (Vectastain Elite ABC Elite 1:500, Vector Laboratories Inc, Burlingame, US) and the GnRH-immunoreactivity was developed in 0.05% DAB /0.005% H2O2 in 0.05 M Tris buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200; Vectastain standard kit, Vector Laboratories, RRID:AB_2336819). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were labeled with DAPI (1:5000 in PBSTx) overnight at 4°C and mounted with Vectasheild (CAT H-1000-10; Vector Laboratories).
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bioRxiv - Microbiology 2024Quote: ... The fixed homogenate was stained with a 1:150 dilution of FITC-conjugated Dolichos biflorus agglutinin (Vector Laboratories cat# FL-1031) in 1X PBS and slowly rotated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... Endogenous peroxidase and biotin were blocked with 1% hydrogen peroxide-methanol for 10 min and a biotin blocking kit (Vector Laboratories), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... was carried out according to manufacturer’s protocol after fixation of organoids with 0.1% paraformaldehyde in PBS for 1 hour at room temperature followed by blocking with Avidin/Biotin (Vector Labs SP-2001). Biotin-Maackia Amurensis lectin was labeled with streptavidin-PE conjugate (Thermo Fisher SA10041 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Triton-X-100) four times for 20 min each and kept in Vectashield antifade mounting medium with DAPI (Vector laboratories; H1200), at 4 °C.
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... Rinsing with PBS was followed by incubation in a solution containing biotin-conjugated horse anti-goat IgG (1:200 (Vector Laboratories, USA) in 3 % horse serum + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories, PK-6100) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800; Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit, Vector Laboratories, PK6100). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were stained using Wisteria floribunda agglutinin coupled to fluorescein (WFA; 1:100; cat# FL-1351, RRID: AB_2336875, Vector Laboratories, CA, USA) for 12h (at 4°C) ...
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxide blocking was performed in 1% water for 15 minutes before blocking in 2.5% normal horse serum (Vector Laboratories -MP-7401) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 1h incubation at room temperature with a 1:250 dilution of an avidin-biotin complex solution (ABC Vectastain kit, Vector Laboratories®). Revelation was performed using the DAB Peroxidase Substrate Kit (DAB SK4100 kit ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue sections were rinsed again in TS and then incubated in biotinylated WFA (Vector Laboratories, Cat# B-1355, RRID: AB_2336874, 1:50) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... RGCs were purified using two-step immune-panning on two Lectin coated negative panning plates (Bandeiraea simplicifolia, BSL-1; Vector Laboratories Ltd.) and a Thy1.2 antibody coated positive plate (Serotec MCA02R ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue sections were rinsed again in TS and then incubated in biotinylated WFA (Vector Laboratories, Cat# B-1355, RRID: AB_2336874, 1:50) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed again (3 times in PBS/Tween, 5 minutes each) and incubated in biotinylated goat anti-rabbit (1: 200, Vector Laboratories, USA) or biotinylated horse anti-mouse (1 ...
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bioRxiv - Neuroscience 2021Quote: The sections were blocked for 1 h at room temperature in 0.5% Triton-X100 in PBS (PBST) and 10% normal goat serum (Vector Laboratories, S-1000) solution ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were permeabilized with 0.5% Triton X-100 for 30 min at room temperature and blocked in 5% goat serum (Vector Laboratories, 1:200), goat anti-rat 488 and goat anti-mouse 647 (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti-Ntla immunostaining was performed after in situ hybridization using rabbit anti-Ntla (Schulte-Merker et al., 1992, 1:2000) and Horse anti-rabbit IgG-HRP conjugated (Vector Laboratories, Inc.)
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Non-specific binding sites were blocked for 1 hour (Col2: MoM Kit, Vector Labs, BMK-2202; Sox9: 10% v/v goat serum). Primary antibodies were incubated overnight at 4°C and included Col2 (1/100 ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories,UK) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... blocked for 1-hr in kit-specific blocking reagent (ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Detection Kit, Vector Laboratories, MP-7451), and incubated for 24-hr at 4°C with primary antibody (Table S1) ...
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bioRxiv - Immunology 2020Quote: ... and nonspecific epitopes (species-specific serum diluted 1:5 in Tris-buffered saline and 5% bovine serum albumin, or blocking serum from species specific ImmPRESS® kit; Vector Laboratories) and incubated with primary antibody (Table 2 ...
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bioRxiv - Immunology 2021Quote: ... Labeling was also performed with a 1:250 dilution of Lectin agglutinin I (Ulex europaeus UEA-I)-FITC (Vector Labs, Burlingame, CA).
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories, UK) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... we removed the supernatant and added 1 ml ACSF with DAPI (Vector Laboratories, Cat No.: H-1200-10ml, 5 μ g/ml). Collected single-cell suspensions were used for library construction.
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bioRxiv - Neuroscience 2022Quote: ... Sections were blocked for 1 h in 0.3% PBS-Triton-X-100 with 6% normal goat serum (NGS; Vector Labs, S-1000), followed by incubation for 72 h at 4 ºC with anti-cFos rabbit primary antibody (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... slides were thawed in the hood overnight and stained with Dylight 488-conjugated Lycopersicum esculentum lectin (1:100; Vector laboratories; #DL-1174) to visualize brain microvessels ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed in 0.01 PBS and incubated overnight at 4 °C with Alexa biotinylated goat anti-rabbit 488 (1:2000, Vector Laboratories, Burlingame, CA). After rinses ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... A cHL FFPE section was mounted on a No.1 glass coverslip pre-treated with VECTABOND Reagent (Vector laboratories, SP-1800-7) as described above ...