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Citations for Vector Labs :
2301 - 2350 of 2388 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... followed by extensive washes in TBS (3×10 min) and in VECTASTAIN® Elite® avidin–biotinylated horseradish peroxidase complex (Standard) for 1.5 h (1:250 in TBS; Vector Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue sections were peroxidase quenched with 3% H2O2 for 15 min at RT and blocked (10% normal goat serum/PBS) for 1 hour at RT before Avidin-Biotin blocking (Vector Laboratories, Inc). Primary antibodies were diluted in 5% serum(FBS)/4% BSA/0.3% Triton-X/PBS and incubated for 30 min at RT (CD31 @ 1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×106 cells were resuspended in 100 µL FACS buffer (1X PBS + 1% bovine serum albumin + 0.05% sodium azide) and stained for 30 mins on ice with the following lectins at 1:400 dilution: SNA-FITC (Vector Laboratories; RRID:AB_2336719), Mal-I-FITC (Bioworld 21761036) ...
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bioRxiv - Neuroscience 2024Quote: ... slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed and blocked for 1 hour at room temperature in 10% normal goat serum (0.1% Triton X in Tris buffer; Vector Labs, Cat# S2000). Both primary and secondary antibodies were diluted in the same Tris buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Cells treated with extensive washes with PBS with 1% BSA prior to flipping the coverslip and mounting on glass slides in anti-fade media (Vector Laboratories, Inc.). The samples were then imaged using confocal fluorescence microscopy (LSM 710 Confocal ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a biotinylated anti-human IgG in TBST with 1% goat serum also incubated for 30 minutes at room temperature (Vector Labs, Burlingame, CA). Then ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti rat (3052-08)) for 1 hour at room temperature and labelled structures were visualized using the ABC Vectastain kit (Vector laboratories, PK-6100). Finally ...
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bioRxiv - Genetics 2021Quote: ... the gonads were again washed three times with PBSTw and resuspended in 1 drop of Vectashield containing 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA). The gonad was finally mounted on pads of 5% agarose in water on microscope slides and covered with a cover glass (Sulston and Horvitz ...
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bioRxiv - Developmental Biology 2020Quote: ... was incubated for 1 hour at room temperature followed by a 30-minute incubation with Avidin-Biotin complex (ABC kit, Vector laboratories Cat # PK6100). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... consisting of PBS containing 1% (w/v) Triton X-100 (A1388, AppliChem) and 10% (v/v) normal goat serum (S-1000, Vector Labs, Burlingame, CA) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Microbiology 2020Quote: ... The coverslips containing the immuno-stained cells were washed thrice with 1 x PBS then mounted onto glass slides using mounting media with DAPI (Vector Laboratories, Inc., USA). The stained cells were viewed using confocal laser scanning microscopy (CLSM ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... Then the sections were incubated for 24 h (at RT with gentle shaking) with the mixture of Wisteria floribunda agglutinin (WFA) (1:500, Vector Laboratories, B-1355) and mouse anti-Tenascin-R (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then reacted with an avidin-biotin conjugate (ABC kit) for 1 hour and visualized using the chromogen VIP (Vectastatin Elite ABC kit and Vector VIP kits; Vector Labs, Burlingame, CA).
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then rinsed with HPLC-grade acetone and immerged into 1% (v/v) Vectabond (product code #SP-1800, Vector Labs, CA, USA) in acetone for 10 min to functionalise the glass surface with amino groups ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Physiology 2020Quote: ... The slides were then incubated for 1 hour in a humidity chamber in a mixture of goat anti-rabbit peroxidase conjugated (Vector Labs PI-1000). and goat anti-chicken Alexafluor 488 conjugated (Abcam #ab150169) ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated in the second antibody solution for 1 h at 37° C and subsequently mounted in Vectashield mounting medium containing 4′,6-diamidin-2-phenylindol (DAPI, Vector Laboratories, Burlingame, California) in a manner that conserves the 3D structure of the specimen [42] ...
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bioRxiv - Microbiology 2020Quote: ... Cells were counterstained with VECTASHIELD® Antifade Mounting Medium containing 1.5 µg ml- 1 4,6-diamidino-2-phenylindole (Vector Laboratories, Burlingame, CA, USA). Images were captured on a BX61 microscope equipped with a DP80 camera (Olympus Australia ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories, Burlingame, CA). Slides were developed with Bio-Red (Biopath ...
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and stained overnight at 4C with FITC-conjugated IB4 (Fluorescein labeled Griffonia Simplicifolia Lectin I isolectin B4, FL-1201, Vector laboratories, 1:200) or primary antibody against Cld-5 (mouse monoclonal ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:200 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500). Imaging was performed on a Leica SP8 Confocal microscope using a 40x oil objective ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and stained with 1:20 diluted fluorescein-conjugated UEA-I (Lectin Kit I, Fluorescein, UEA-I-FL, #FKL2100; Vector Laboratories, Burlingame, CA, USA). The cells were washed with PBS containing 0.2% FBS and subjected to flow cytometry by a Moxi GO II cell analyzer (Orflo ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 × 10 min with PBS and incubated in a tertiary of avidin and biotinylated horseradish peroxidase (1:1000; ABC kit, Vector Labs, PK-6100) and stained with a 3 ...
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bioRxiv - Neuroscience 2022Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Cancer Biology 2022Quote: ... CDX tumors were grown to approximately 750 mm3 and mice received an i.v injection of Biotinylated Tomato Lectin (4mg/kg, Vector Laboratories, B-1175-1) one hour before they were sacrificed by Schedule 1 method ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used for immunohistochemistry included biotinylated goat anti-rabbit (BA-1000) and biotinylated horse anti-goat (BA-9500) at a concentration of 1:200 (Vector laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated in the presence of biotinylated goat anti-rabbit IgG followed by Vectastain elite ABC reagent (1:600, #PK6101, Vector Laboratories, Burlingame, USA). Fos-like immunoreactive cells were visualized with the avidin-biotin peroxidase method using diaminobenzidine as a chromogen ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... at 1:500 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500).
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in a solution containing avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories; 1:50) for 2hrs ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: ... were used as described previously (Kantar et al., 2021) for 1 hour at room temperature before mounting the coverslips with Vectashield (Vector Laboratories #H-1200) and imaging on Zeiss Apotome or Leica Thunder microscopes.
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times in PBS and 2 times in 0.1 M phosphate buffer solution (10 min/wash) before being mounted and cover-slipped using Vectashield Vibrance w/DAPI (Vector Laboratories H-1800). Fluorescent conjugates of streptavidin were stored and prepared as recommended by the manufacturer ...
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bioRxiv - Physiology 2024Quote: ... The tissues were counterstained with 1 μg/ml DAPI and slides were mounted in Vectashield Vibrance antifade mounting media (Vector Laboratories, H-1700). Stained tissues were imaged on confocal Zeiss 710 microscope operated by ZEN software (Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at RT and initiation of color reaction with a Peroxidase Substrate Kit (SG Peroxidase Substrate Kit, Vector Laboratories, SK-4700). All stained sections were mounted ...