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Citations for PerkinElmer :
551 - 600 of 755 citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% Sarkosyl, 2.5 mM MgCl2, 0.5 mM DTT, 0.05#mM biotin-A/C/G/UTP [Perkin Elmer], 0.4 u/μl RNase inhibitor). The total RNA was isolated with Trizol LS (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Cell Biology 2022Quote: PDE activity was measured at 30°C with the two-step method described by [48] using [3H]cGMP (Perkin Elmer, MA, USA). Aliquots of eluted fraction from SEC were incubated in 60 mM HEPES pH 7.2 assay buffer containing 0.1 mM EGTA ...
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bioRxiv - Immunology 2020Quote: ... The pyroptotic process was monitored at 34°C and 7.5% CO2 using a fully automated spinning disk confocal microscope (PerkinElmer Technologies, OPERA QEHS) with a 40x water immersion objective (Aulner et al. ...
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bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
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bioRxiv - Plant Biology 2020Quote: ... The reaction was incubated for four hours at 37°C and the results were visualised using the LabChip GX Touch 24 (PerkinElmer, Waltham, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
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bioRxiv - Immunology 2022Quote: ... and the reaction was continued for 24 hours at 37 °C before FP measurement using a Victor X5 Multilabel plate reader (PerkinElmer, Shelton, CT). DP4.1-Clip (250 nM) ...
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bioRxiv - Microbiology 2023Quote: ... in 96-well plates in 28°C with shaking and the OD600 measurements were performed hourly using an EnVision Multilabel Reader (Perkin Elmer, USA).
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bioRxiv - Cell Biology 2022Quote: ... Nascent proteins are labeled during translation reactions of 15-30 min at 32°C in RRL by incorporation of radioactive 35S-methionine (Perkin Elmer, USA). Nascent TA proteins were released from the ribosome with 1 mM puromycin and then incubated with 5% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
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bioRxiv - Molecular Biology 2024Quote: ... This RNA unwinding assay was performed at 37 °C in 384-well plates using a Victor Nivo plate reader (PerkinElmer, Basel, Switzerland). The excitation wavelength was set to 640 nM and the emission was measured at 685 nm ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was read approximately 2 min later using a Wallac Victor3 plate reader (Perkin Elmer). 50% ADCC titers were estimated as previously described for virus neutralization assays (41) ...
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bioRxiv - Cell Biology 2021Quote: ... and 500 µM ATP (with 2 μCi of [γ-32P] ATP added per reaction; PerkinElmer BLU502Z250UC). Phosphorylated proteins were separated on 10% SDS-PAGE gels unless stated otherwise ...
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bioRxiv - Physiology 2020Quote: ... Cells were incubated with 50 μM 2DG containing 0.25 μCi 3H-2-deoxyglucose (2DG, Perkin Elmer) for 5 min and washed three times with cold PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Isolated cardiac myocytes were incubated with 0.1 mM [1,3-3H]glycerol (2 μCi/dish, Perkin Elmer) or 0.1 μM [1-14C]linoleic acid (2 μCi/dish ...
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bioRxiv - Neuroscience 2023Quote: ... Isotope content was determined in 2 ml Ultima Gold™ XR scintillation liquid (6013119, Perkin Elmer) using the Tri-Carb 2900TR Liquid Scintillation Analyzer (Packard) ...
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bioRxiv - Cell Biology 2024Quote: ... The organs were harvested and imaged using the IVIS Lumina XRMS Series 2 instrument (PerkinElmer, USA) to assess the biodistribution of mannose ...
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bioRxiv - Bioengineering 2019Quote: ... were analysed based on the Mehlich-3 extraction procedure (Mehlich, 1984) preceding inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 800, Winlab 5.5, PerkinElmer Inc.,Waltham, MA, USA). Exchangeable acidity (H + Al ...
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bioRxiv - Microbiology 2021Quote: ... The volumes of internalised DNA in GFP expressing bacteria were estimated by 3-D image analysis performed using Volocity software (Perkin Elmer, Waltham, USA).
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bioRxiv - Cell Biology 2021Quote: ... 41.3 Ci mmol−1) and radiocarbon-labelled serotonin binoxalate (14C-serotonin; 54.0 mCi mmol−1) were purchased from Perkin Elmer (Waltham, Massachusetts, USA). Serotonin creatinine sulphate ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were generated in 3 dimensions and minimized by applying the MM2 energy-minimization method in ChemBio3D (ChemDraw® Professional v20, PerkinElmer Informatics, Inc). The minimized structures were overlaid by steric fields and the similarity was calculated in Discovery Studio Visualizer v21.1 (Dassault Systèmes Biovia Corp).
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bioRxiv - Molecular Biology 2020Quote: ... The 3’TAMRA fluorescence from 5’UAR was recorded by a single molecule avalanche photodiode (SPAD) (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 600/50 band pass emission filter ...
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bioRxiv - Cancer Biology 2023Quote: ... culture plates were incubated for 3 days after which they were imaged with an Operetta CLS high-content analysis system (Perkin Elmer, LIVE configuration) with the incubation chamber set to 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Colorimetric or fluorimetric quantifications of the respective readouts were done in a multiwell plate reader (Wallac Victor 3 V, PerkinElmer, Waltham, MA, USA) and conducted in white 96-well plates.
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bioRxiv - Microbiology 2021Quote: ... The mixture was incubated at 37 °C for 20 min before the RFU of fluorescent signals were read on an Envision microplate reader (Perkin Elmer, Waltham, MA) with excitation wavelength set at 320 nm and emission wavelength set at 400 nm ...
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bioRxiv - Physiology 2020Quote: ... Progesterone was extracted with ethyl ether after overnight equilibration (4°C) of the plasma with 1500 dpm of tritiated progesterone (Perkin Elmer, Rodgau, Ermany). Separation of the organic and aqueous phase was conducted similar to the procedure described for the other hormones ...
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bioRxiv - Microbiology 2020Quote: ... Amastigotes were incubated at 37°C for 72 hours followed by lysis and measurement of luminescence using the ATPlite assay (Perkin Elmer, Waltham, MA).
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bioRxiv - Microbiology 2023Quote: ... was added to bacterial supernatants and the enzymatic activity was monitored for 30 min at 37°C (380 nm) in a VICTOR2 fluorometer/spectrophotometer (Perkin Elmer, Waltham, MA). The enzymatic activity ([PNA generated (nM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... DPBS samples were diluted in 2% HNO3 and plasma samples were digested over night at 70°C in the 1:1 mixture of 70% HNO3 and H2O2 and analyzed using ICP-MS (Perkin Elmer NexION 300D) to determine the concentration of silver within each sample ...
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bioRxiv - Biochemistry 2024Quote: ... 2B were prepared in ICM (Molsoft LLC., San Diego, CA). Chemical structures (Fig. 5A, C) were generated using ChemDraw (PerkinElmer informatics, Waltham, MA).
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 10 min in Cy3 Plus Amplification Reagent diluted 1:300 in 1x Plus Amplification Diluent (TSA Plus Cyanine 3 System, Perkin Elmer, Waltham, MA, NEL744001KT), washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
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bioRxiv - Biophysics 2021Quote: ... emission 535 nm) measured for 1 hour at 2 minute intervals on an EnVision Xcite 2104 (PerkinElmer). Enzymatic activity of each mutant was measured with (+ Palm-CoA ...
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bioRxiv - Immunology 2019Quote: ... 2 × 103 cells were plated in pre-coated 96 well plates (CellCarrier-96 Ultra Microplates, black, PerkinElmer) following the same procedure.
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bioRxiv - Systems Biology 2021Quote: ... columns 2-23 were used) of tissue-culture treated black CellCarrier-384 Ultra Microplates (Perkin Elmer 6057302) using a 12-channel electronic multichannel 200 μL pipettor [Sartorius] ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc, Perkin Elmer) in 15μL reactions ...
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bioRxiv - Cell Biology 2020Quote: ... and the wells were covered immediately using filter papers soaked with hyamine hydroxide (Perkin Elmer, 2-19361). After incubation in a fume hood for at least 12 hours to reach saturation ...
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bioRxiv - Cell Biology 2020Quote: ... and the wells were covered immediately using filter papers soaked with hyamine hydroxide (Perkin Elmer, 2-19361). After incubation in a fume hood for at least 12 hours to reach saturation ...
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bioRxiv - Immunology 2022Quote: ... Uptake was initiated by addition of 1 μCi [3H]2-DG (25-30 Ci/mmol, PerkinElmer, NET549) and 0.1 mM unlabeled 2-DG (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were loaded with 2 μCi/ml D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET50100) in MEM +HIHS cell culture medium ...
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bioRxiv - Developmental Biology 2023Quote: ... Anesthetized (2% isoflurane vaporized in 1 L/min O2) dams were imaged (IVIS Lumina III, Perkin Elmer) with their ventral side facing the charge-coupled device camera (15 cm field of view ...
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bioRxiv - Plant Biology 2023Quote: ... resuspended in 2 N NaOH and mixed with 5 volumes scintillation fluid (Ultima Gold™ XR, PerkinElmer) for 14C-CPM counting in a TRI-CARB liquid scintillation counter (PerkinElmer ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µL of cell culture medium or standard solution was added to an Optiplate-384 microplate (PerkinElmer). 21µL of 10X mixture including acceptor beads and biotinylated antibody was then added to the wells with culture media or standard solution ...
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bioRxiv - Cell Biology 2023Quote: ... cells on the SPA plate were suspended in 1 mL of DDW with 2% glusulase (PerkinElmer, #NEE154001EA) and digested with shaking at room temperature for 3 hr ...
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bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...