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Citations for PerkinElmer :
451 - 500 of 755 citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were incubated in the dark for 1 hr at 37°C followed by measurement of fluorescence using an Envision plate reader (PerkinElmer) with standard AlphaScreenTM settings ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was sealed with parafilm and placed in a pre-heated (37°C) Opera Phenix microscope with 40x or 60x water-immersion lens (PerkinElmer) with 5% CO2.
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Bioengineering 2023Quote: ... of approximately 5.7 ± 3.0 pmol/mg protein measured by the manufacturer using [3H]-DAMGO (ES-542-C OP3 ValiScreen, PerkinElmer, Waltham, MA) were obtained and cultured for a series of binding assays ...
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bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2023Quote: ... Live imaging was performed with a Nikon TE2000 microscope at 37°C using Volocity software (Nikon Corporation, Tokyo, Japan; PerkinElmer). Images were captured with 488 nm excitation and 516 nm emission at a rate of 20 images per second for 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... Live cell imaging was performed at 37°C in 5% CO2 for 24 hours using the Opera Phenix High Content Screening System (PerkinElmer). Acquired images were analyzed by using the Harmony software (PerkinElmer).
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bioRxiv - Cancer Biology 2024Quote: ... was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer). For longer-term proliferation assays ...
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bioRxiv - Microbiology 2023Quote: ... Plates were read once every 30 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 550 and 591 nm ...
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bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were incubated for 4 h at 37°C and then the supernatant was removed and incubated with scintillation fluid (PerkinElmer) overnight at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer], 3H uridine [0.1 μCi, Perkin Elmer] or 3H thymidine [0.32 μCi ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer] ...
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bioRxiv - Cell Biology 2024Quote: Images of living embryos were acquired at 23°C using a PerkinElmer ERS spinning disk confocal system mounted on a Zeiss Axiovet 200M microscope using Volocity software (PerkinElmer). A 63X ...
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bioRxiv - Cell Biology 2019Quote: ... 3 OD600 units of early log phase cells were labeled with 80 mCi of L-[35S]-methionine/cysteine mix (Perkin Elmer). Immunoprecipitated proteins were separated on SDS-PAGE and exposed to phosphor screens ...
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bioRxiv - Cancer Biology 2020Quote: ... the extent of lung metastasis was measured every 3 days for 12 days using non-invasive imaging with IVIS Lumina III (Perkin Elmer). In brief ...
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bioRxiv - Developmental Biology 2019Quote: ... were used to detect labeled probes by fluorophore deposition (Fluorescein or Cyanine 3) using the TSA Plus System (Perkin Elmer, NEL753001KT). Nuclei were stained with Hoechst 33342 (Thermofisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... Both supernatant and lysate were moved to separate counting vials and mixed with 3 mL scintillation fluid (Ultima Gold #6013321, Perkin Elmer). 14C levels were measured in each sample using a Packard Liquid Scintillation Analyzer ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was transferred to a tube and the protein-bound [α-32P]dCTP was quantified in a Beckman Coulter LS 6500 Liquid Scintillation Counter using OptiPhase HiSafe 3 (PerkinElmer) as solvent.
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bioRxiv - Bioengineering 2021Quote: ... Mice were administered 3.3 mg D-Luciferin in the morning of day 3 and luminescence was recorded after 10 minutes by an In Vivo Imaging System (IVIS, Perkin Elmer) followed by i.v ...
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bioRxiv - Neuroscience 2020Quote: ... was used as a secondary fluorophore for C1 probes (Gtpbp2, Slc7a1, Chac1) and TSA® Plus Cyanine 3 (PerkinElmer, 1:2,000) was used as a secondary fluorophore for C2 probes (Gtpbp1 ...
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bioRxiv - Microbiology 2022Quote: ... The bound calcofluor was measured at excitation (λEx) 350 nm and emission (λEm) of 475 nm with VICTOR 3 1420 Multilabel Counter (PerkinElmer, USA).
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bioRxiv - Microbiology 2022Quote: ... Animals were anaesthetized using an oxygen-filled induction chamber with 3% isoflurane and bioluminescence was measured using the in vivo imaging system IVIS (IVIS Spectrum Imaging System, Perkin Elmer). Mice were whole-body imaged in dorsal position and the signal intensity was obtained from luciferase expressed in T ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Cell toxicity was assessed after 3 h of incubation by measuring fluorescence intensity using the Victor 3V spectrofluorometric multiwell plate reader (Perkin Elmer; excitation at 560 nm ...
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bioRxiv - Microbiology 2020Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [a32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2020Quote: ... the cultures added with 0.05% v/v DCPK and fluorescent emission for the next 3 hours followed as described in detail in (21) with a Gallios (Perkin Elmer) flow cytometer ...
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bioRxiv - Microbiology 2020Quote: ... The dry filter paper was exposed to film for imaging before the individual dots on the filter paper were excised and scintillated with the addition of 3 ml scintillation fluid (Perkin Elmer). Radioactive counts were measured as scintillation counts per minute.
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bioRxiv - Immunology 2022Quote: ... β-radioactivity was measured by liquid scintillation counting in a Beckman LS 6500 Multi-Purpose Scintillation Counter (scintillant Optiphase HiSafe 3; PerkinElmer, #1200.437). L-Alanine (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [α32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... 12 pictures of at least 3 biological replicates were quantified using the Harmony high-content imaging and analysis software (Perkin Elmer).
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bioRxiv - Microbiology 2023Quote: ... Oligonucleotide probes (listed in Supplementary file 3) for the different RNAs were labelled with 0.3 mCi of [γ-32P] ATP (Perkin Elmer) by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Pathology 2023Quote: ... as previously described [71] using Multiplex Fluorescent Reagent Kit V2 (Advanced cell Diagnostics) and TSA Plus Cyanine 3 and Cyanine 5 (Perkin Elmer) as fluorophores according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: NMuMG cells expressing either EGFP-BIK-L61G or EGFP-PUMA4E were seeded (3,000 cells per well) in multiple wells (at least 3) of a 384-well microplate (CellCarrier-384 ultra, B128 SRI/160; Perkin Elmer) and allowed to grow for 24 h before staining with the nuclear dye DRAQ5 (5nM ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times for 5 min in TBS-T buffer and prior to chemiluminescence detection using Western Lightning chemiluminescent substrate (Perkin Elmer). Chemiluminescence signal was detected with an ECL-Imager (INTAS ...
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bioRxiv - Biochemistry 2023Quote: ... 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 μL 14C-labelled lipids from the denser organic fraction were combined with 4 mL OptiPhase HiSafe 3 liquid scintillation cocktail (PerkinElmer; 1200.437) and radio-labeling was quantified using a Tri-Carb 2910 TR Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
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bioRxiv - Cell Biology 2024Quote: ... After two washes of 5min in 1X RNAscope wash buffer slides were incubated with 1:1500 Cyanine 3 amplification reagent (PerkinElmer, #FP1170012UG), or 1:1000 Cyanine 5 amplification reagent (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Further 160μl of DNS solution was added in each well and incubated at 100°C for 20 min followed by reading at 570nm in multimode plate reader (Perkin Elmer). All the quantitative assays were done in triplicates and the activity was estimated using the standard graph of glucose.
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bioRxiv - Biophysics 2019Quote: ... The plate was then incubated for 20-48 hours at 30°C in a Victor X3 multimode plate reader (Perkin Elmer). Every 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were then rapidly filtered under vacuum through GF/C glass fiber filter-bottom 96-well microplates (Perkin Elmer, MA, USA) pre-treated with 0.1% polyethyleneimine (PEI ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2019Quote: ... AMC fluorescence released by cleavage of substrate by proteolytic activity was recorded in a black 96-well plate after 60 min incubation at 37°C using the Victor 3V Multilabel counter (Perkin Elmer). Specific proteasomal activity was determined by subtracting unspecific AMC fluorescence obtained in the presence of proteasome inhibitor MG132 (20 μM) ...
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bioRxiv - Biochemistry 2021Quote: ... Complexes were incubated for 1h at 37°C and luminescence released by the [14C]-RITA-excited beads was measured in standard microplate reader (Perkin Elmer).
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bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
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bioRxiv - Cell Biology 2020Quote: ... and recorded on a 37°C heated stage of Nikon Ti inverted microscope using Ultraview spinning disk confocal system (Perkin Elmer). For the TEM opening ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...