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Citations for PerkinElmer :
351 - 400 of 531 citations for Fmoc S 3 amino 4 3 4 dichloro phenyl butyric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 41.3 Ci mmol−1) and radiocarbon-labelled serotonin binoxalate (14C-serotonin; 54.0 mCi mmol−1) were purchased from Perkin Elmer (Waltham, Massachusetts, USA). Serotonin creatinine sulphate ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Donor and acceptor emission were measured at 3 min intervals (during a total of 66 min) at 37°C with an EnVision 2014 Multilabel Reader (PerkinElmer, Waltham, MA, USA) using a 340/60 nm excitation filter and 520/8 nm (acceptor ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were generated in 3 dimensions and minimized by applying the MM2 energy-minimization method in ChemBio3D (ChemDraw® Professional v20, PerkinElmer Informatics, Inc). The minimized structures were overlaid by steric fields and the similarity was calculated in Discovery Studio Visualizer v21.1 (Dassault Systèmes Biovia Corp).
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bioRxiv - Molecular Biology 2020Quote: ... The 3’TAMRA fluorescence from 5’UAR was recorded by a single molecule avalanche photodiode (SPAD) (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 600/50 band pass emission filter ...
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bioRxiv - Cell Biology 2023Quote: ... Colorimetric or fluorimetric quantifications of the respective readouts were done in a multiwell plate reader (Wallac Victor 3 V, PerkinElmer, Waltham, MA, USA) and conducted in white 96-well plates.
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bioRxiv - Cancer Biology 2023Quote: ... culture plates were incubated for 3 days after which they were imaged with an Operetta CLS high-content analysis system (Perkin Elmer, LIVE configuration) with the incubation chamber set to 37 °C and 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... 0.5 mM S-adenosyl-L-[methyl3H] methionine (SAM) (Perkin Elmer), 0.4 μM (GTmC)12/(GAC)12 hemimethylated DNA duplex ...
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bioRxiv - Physiology 2020Quote: ... [9,10-3H(N)]-oleic Acid (PerkinElmer) or n-[2,2’,3,3’-3H] octanoic acid (American Radiolabeling Chemicals ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2021Quote: ... diluted in buffer A to a final concentration of 4 nM) was added to a 384-well microplate (OptiPlateTM-384 Plus, Perkin Elmer) followed by the addition of 15 μL diluted proteins and 5 μL of the GFP antibody-conjugated acceptor beads (5x) ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Plant Biology 2022Quote: ... 5-mm root segments away from the apical 2 mm root tip and 2 mm away from the root-shoot junction were carefully cut and soaked overnight in 4 ml of liquid scintillation fluid (Ultima Gold, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Physiology 2020Quote: ... Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by: addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and the retained radioactivity determined using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were cut as 4 μm sections and automated IF was performed on a Leica Bond Rx Platform at room temperature using the PerkinElmer Opal 4-Colour Automation IHC Kit (PerkinElmer, #NEL800001KT). Tissue sections were blocked with 3% Hydrogen peroxide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and counted for 2 min after a 4 hr delay on a Perkin Elmer Betaplate 1205 liquid scintillation counter (Perkin Elmer). Nonspecific binding was determined with 1.0 µM dihydroergotamine.
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were incubated for four hours at 4 °C in the presence of benzazepine-red nonpeptide vasopressin antagonist (BZ-DY647, PerkinElmer Revvity) at various concentrations ranging from 10-10 to 10-7 M ...
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bioRxiv - Molecular Biology 2024Quote: ... and were then anesthetized (isoflurane 4 % for induction and 1.5 % for maintenance) before placing in the optical imaging system (IVIS Lumina III, PerkinElmer, Courtaboeuf, France). In vivo bioluminescence signals were measured in selected regions of interest (injected testes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 10 min in Cy3 Plus Amplification Reagent diluted 1:300 in 1x Plus Amplification Diluent (TSA Plus Cyanine 3 System, Perkin Elmer, Waltham, MA, NEL744001KT), washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer; #NEG772002MC) for radiography detection or with untagged Methionine (Promega #L118A ...
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bioRxiv - Biochemistry 2023Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer)] ...
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bioRxiv - Genetics 2024Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer) in a reaction volume of 10 or 20 µL (in 50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2024Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer) in a reaction volume of 20 µL (in 50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer) in a reaction volume of 20 µL (in 50 mM Tris-Cl pH 9.0 ...
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bioRxiv - Biochemistry 2024Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer) in a reaction volume of 20 µL (in 20 mM Tris-Cl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer) in a reaction volume of 20 µL (in 50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2024Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer) in a reaction volume of 20 µL (in 50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 µM S-[methyl-14C]-adenosyl-L-methionine (PerkinElmer, #NEC363050UC). Reactions were incubated in reaction buffer (50 mM Tris-HCl pH 8.0 at 30°C ...
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bioRxiv - Pathology 2022Quote: Fatty acid uptake and incorporation into lipids as well as de novo lipogenesis were determined using [9,10-3H(N)]-oleic acid and [1,2-14C]-acetic acid (PerkinElmer), respectively ...
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bioRxiv - Microbiology 2023Quote: Measurements of fatty acid biosynthesis were performed by measuring the incorporation of (1-14C) acetic acid (Perkin Elmer) into cellular lipids as described previously(Spencer et al. ...
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bioRxiv - Physiology 2021Quote: ... 4.5µCi 3H-oleic acid (NET289001MC, Perkin Elmer), was dried down under N2 then resuspended in PBS and combined 1:1 with 40% fatty-acid free bovine serum albumin to a final volume of 200µl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... on a Chemagic360 nucleic acid extractor (PerkinElmer) with a 96-well rod head after samples were homogenized using a steel ball in lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... [U-14C]palmitic acid (Perkin Elmer, NEC534050UC), 100 µM cold palmitic acid (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... [2-14C] acetic acid (Perkin Elmer, NEC553050UC) and 50 µM cold acetic acid (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... where reactions were incubated with 150 nM of the gel-filtration purified WT mouse Naa10 or Naa12 in a 30 µl reaction volume containing each 250 µM substrate peptide and radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences) for 12 min (Naa12 ...
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bioRxiv - Microbiology 2022Quote: ... were performed in a HORIBA JOBIN YVON Spectrofluorometer (FluoroMax – 4) (HORIBA Scientific, Edison, New Jersey, USA) in a fluorescence cuvette of path length 5 mm (Perkin Elmer B0631123), after putting 0.5ml of the sample solution in it ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Half of the cell Triton X lysate from each well was pipetted into a scintillation vial and 4 ml of scintillation fluid (#6013329, Ultima GoldTM, Perkin Elmer, USA) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared in in vitro transcription reaction on linear PCR-generated template containing RNAImod (4) with 500 μM ATP or NAD and 25 μM [α 32P] CTP (Perkin Elmer) using hexa-histidine tagged E ...
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bioRxiv - Microbiology 2024Quote: ... Mixtures were incubated for 60 min at room temperature prior to adding 4 µl of D-luciferin (30 mg/ml) (Perkin Elmer; #122799). After additional incubation for 5 min ...