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Citations for PerkinElmer :
301 - 350 of 531 citations for Fmoc S 3 amino 4 3 4 dichloro phenyl butyric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: NMuMG cells expressing either EGFP-BIK-L61G or EGFP-PUMA4E were seeded (3,000 cells per well) in multiple wells (at least 3) of a 384-well microplate (CellCarrier-384 ultra, B128 SRI/160; Perkin Elmer) and allowed to grow for 24 h before staining with the nuclear dye DRAQ5 (5nM ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times for 5 min in TBS-T buffer and prior to chemiluminescence detection using Western Lightning chemiluminescent substrate (Perkin Elmer). Chemiluminescence signal was detected with an ECL-Imager (INTAS ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatants were transferred to 3 ml liquid scintillation liquid and analyzed using a Tri-Carb 2810 TR scintillation counter (Perkin Elmer).
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bioRxiv - Biochemistry 2024Quote: ... 200 μl of the supernatants were transferred to 3 ml liquid scintillation and analyzed using a Tri-Carb 2810 TR scintillation counter (Perkin Elmer).
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bioRxiv - Cell Biology 2024Quote: ... The plates were incubated in the dark for 30 minutes and then read for fluorescence using a Victor 3 microplate reader (Perkin Elmer) with excitation/emission detection at 485 nm/530 nm ...
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bioRxiv - Biochemistry 2023Quote: ... [14C]-palmitic acid (Perkin Elmer) was added to a final activity concentration of 0.2 μCi/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were split again on days 4 or 5 as needed into a 96-well imaging plate (Perkin Elmer). A half media change was performed every other day if cells were not being split ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Microbiology 2022Quote: ... one ml of culture was labelled for 4 min at 30 °C using 30 μCi γ-[32P]ATP (PerkinElmer). Samples were incubated with 6% sucrose ...
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bioRxiv - Immunology 2023Quote: ... uptake inhibition and degradation were determined in M[0] and M[IL-4] using 3H-choline chloride (Perkin Elmer) as previously described [4].
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4 μL/well of cell culture supernatant was taken out and dispensed into OptiPlate-384 microplates (PerkinElmer, cat #: 6007290) that was pre-filled by 12 μL/well PBS by an EL406 ...
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bioRxiv - Cell Biology 2022Quote: Candidate small RNA sequences were identified from raw sequence data using cutadapt (ver. 2.8)21 with parameters “-u -4 -a A(10)” as recommended by PerkinElmer NextFlex Combo-Seq kit ...
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bioRxiv - Immunology 2024Quote: ... Clone 2C.4 was screened against human cardiac myosin and the β-adrenergic receptor (βAR) enriched membranes (Perkin Elmer) by ELISA ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by library preparation with the Next-Flex small RNA v.4 kit protocol (Perkin Elmer, NOVA-5132-06), in accordance to manufacture recommendations ...
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bioRxiv - Microbiology 2024Quote: ... and 4 z positions (0 µm, 0.5 µm, 1 µm, and 1.5 µm) with an Opera Phenix microscope (Perkin Elmer). Colocalization analysis of LC3 ...
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bioRxiv - Biochemistry 2022Quote: ... S-[methyl-3H] (3H-SAM) (PerkinElmer, 18.0 Ci/mmol); and 0.1 μM purified DNMT3A2 in assay buffer (50 mM sodium phosphate ...
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bioRxiv - Synthetic Biology 2021Quote: ... A Swafer S splitter (Perkin Elmer Inc., Wellesley, PA) was employed for this purpose ...
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bioRxiv - Cancer Biology 2023Quote: ... and TSA Plus Cyanine5 (used for BRD4-S) (PerkinElmer, MA ...
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bioRxiv - Cancer Biology 2021Quote: ... For each PI3K isoform a 3 µL mixture (1:1) of enzyme/lipid substrate solution was added to a 384 well white assay plate (Perkin Elmer #6007299) containing 50 µL of test compound or DMSO only for untreated controls ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
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bioRxiv - Molecular Biology 2021Quote: ... The plates were read at a wavelength of 460 nm using the VICTOR 3 V 1420 Multi-Label Microplate Reader (Perkin Elmer, USA). All reactions were carried out in triplicate using both antigenic peptides in separate experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... The reverse primer R3 (5′-GCGCCGGTAGTTGCCCTTCTC-3′) was labeled at the 5′ end using [γ-32P]-ATP (PerkinElmer Inc., Waltham, MA, USA) and T4 polynucleotide kinase (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... Imaging involving mNG-Bud8 (Figs. 3 & S2) was performed using a spinning disk confocal microscope (Ultra-VIEW VoX CSU-X1 system; Perkin Elmer-Cetus) equipped with a 100x /1.45 NA Plan-Apochromat Lambda oil immersion objective lens (Nikon) ...
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bioRxiv - Cell Biology 2021Quote: Time-lapse or live-cell fluorescence microscopy was performed using a Zeiss Axiovert 200 M microscope (Plan Apochromat 1006, 1.4NA objective) with an Ultra-View RS-3 spinning disk confocal system (PerkinElmer Inc., United States) which equipped with a CSU21 confocal optical scanner ...
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bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of each phase were collected and measured in a 2480 automatic Gamma counter Wizard 2 3” (PerkinElmer, Waltham, MA, USA). LogD value were calculated using Excel by dividing measured values of octanol by water and logarithmize the result ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-plex positive control probe or with a cocktail of Abcb5-C1 and Abcb4-C2 probes with a 1:750 dilution of TSA-Fluorescein Plus and TSA-Cyanine 3 Plus (PerkinElmer, Shelton, CT), respectively ...
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
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bioRxiv - Biophysics 2023Quote: ... stand and a 40 nt oligo-dT at the 5′ end was labeled at the 3′ terminus with [α-32P] dCTP (Perkin Elmer) and annealed to the single-stranded DNA M13mp18(24) ...
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bioRxiv - Physiology 2024Quote: GFP fluorescence was monitored before and at several time points after a 3-week doxycycline treatment using an in vivo IVIS Spectrum Imaging System (PerkinElmer, Waltham, MA). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... Cell fixation was performed 24h after transfection using 4% formaldehyde and cellular imaging was performed using an Operetta CLS (PerkinElmer). Three individual plate preparations were used as independent experiments for statistical significance (n=3) ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 4 h at 37°C and fluorescent signal intensity was quantified using the EnSpire Multimode Plate Reader (PerkinElmer) at excitation and emission wavelengths of 540 and 612 nm respectively.
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bioRxiv - Molecular Biology 2020Quote: ... The protein pellet was then re-suspended in PBS and incubated in 4 mL of scintillation fluid (Insta-Gel Plus, PerkinElmer) for 24 h (to homogenize the samples ...
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bioRxiv - Biochemistry 2021Quote: ... 50 nM of hNatD was mixed with 2 μM of radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences), 20 μM of the peptide ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Biophysics 2020Quote: ... separated free and bound radioligand and the radioactivity on each filter determined by the addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and count using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Neuroscience 2022Quote: ... To quantify α1-3;5GABAAR79 sections were incubated for 60 minutes at 4°C in 1nM [3H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [3H]flumazenil with 10 µM flunitrazepam (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in α-MEM containing media containing 2μM Glutamine and 4 mCi/mL L-[3,4-3H(N)]-Glutamine (PerkinElmer, NET551250UC). GLS activity was terminated by washing cells with ice cold HBSS for three times followed by scaping cells with 1 mL ice cold milliQ water ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aerrane (Isoflurane, Baxter) inhalation was performed for anesthesia using the RAS-4 Rodent Anesthesia System (Perkin Elmer, Waltham, MA, USA) for experiments with IVIS Spectrum CT living imaging system (Perkin Elmer ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Neuroscience 2024Quote: ... since this was when significant peripheral neuropathy first became detectable11.Tumor growth was monitored weekly for up to 4 weeks by bioluminescent imaging using an IVIS Lumina XR (PerkinElmer), as described previously11 ...
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bioRxiv - Bioengineering 2024Quote: ... was injected subcutaneously (Figure 2) or intraperitoneally (Figure 4) into mice and bioluminescence imaging was performed using IVIS Lumina series III (PerkinElmer). During the measurement ...
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bioRxiv - Cancer Biology 2021Quote: Bioluminescence Imaging and Quantification: Mice were anesthetized with isoflurane (2-3% in 100% oxygen) and bioluminescence images acquired with an IVIS Spectrum (Perkin Elmer, Waltham, MA) 5 minutes after intraperitoneal injection of Potassium D-Luciferin solution (Perkin Elmer ...
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bioRxiv - Microbiology 2021Quote: ... The volumes of internalised DNA in GFP expressing bacteria were estimated by 3-D image analysis performed using Volocity software (Perkin Elmer, Waltham, USA).