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Citations for PerkinElmer :
301 - 350 of 431 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 100 pmol of DNA was incubated with 2 μL 5mCi [γ32-P]ATP (Perkin Elmer) and 4 μL T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Biochemistry 2021Quote: ... Cell viability was assessed with cellular ATP levels using ATPlite™ kit (6016943, Perkin Elmer) compared to untreated controls ...
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bioRxiv - Biochemistry 2021Quote: ... mixed to 3 μl of γ32P-ATP (3000 Ci/mmol 10 mCi/ml, Perkin Elmer) for 1h at 37°C then purified on denaturing 10% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... oligos were either 5’-end labelled with [γ-32P]-ATP (3000 Ci/mmol, Perkin Elmer) and T4 PNK (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... ∼80 nM [a-32P]-labelled ATP (3000 Ci/mmol 10 mCi/mL, 250 µCi; PerkinElmer), and 33.3 µg/mL poly(I:C ...
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bioRxiv - Molecular Biology 2023Quote: ... The ATP content was measured at various time points by ATPlite Luminescence Assay System (PerkinELmer). Luminescence was measured by the BioTek SynergyTM Mx plate reader ...
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bioRxiv - Biochemistry 2023Quote: ... 100 pmol of DNA was incubated with 2 μL 5 mCi [γ32-P]ATP (PerkinElmer) and 4 μL T4 PNK (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... in the presence of [α-32P] ATP (10mCi/mL, 3,000 Ci/mmol; Perkin Elmer, BLU512H250UC). After PAGE purification ...
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bioRxiv - Physiology 2022Quote: ... Reactions were started by the addition of 4 mM ATP and 45CaCl2 (Perkin Elmer, NEZ013001MC) to assay buffer with sample ...
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bioRxiv - Physiology 2024Quote: Prdx4 RNA oligo was labeled with [32P]ATP (adenosine 5’-triphospate, [γ-32P]; NEG002A; PerkinElmer) using T4 polynucleotide kinase (M0201 ...
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bioRxiv - Biochemistry 2024Quote: ... the linearized substrates were labeled at both 5′ ends using [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (NEB).
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bioRxiv - Neuroscience 2020Quote: ... as well as Cyanine 3 Tyramide Reagent and Cyanine 5 Tyramide Reagent and Fluorescein Tyramide Reagent (all PerkinElmer), respectively ...
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bioRxiv - Zoology 2022Quote: ... The horseradish peroxidase-conjugated Avidin-Biotin Complex was visualized using TSA Plus Fluorescein System (PerkinElmer, Waltham, MA, USA) or 3,3-diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2020Quote: ... we used a different protocol with the tyramide signal amplification kit (TSA Plus Fluorescein System; PerkinElmer Life, NEL774). Briefly ...
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bioRxiv - Genetics 2021Quote: ... Probes were end-labelled [1 pmol] using 10 μCi of [γ-32P]-ATP (PerkinElmer, Waltham, MA) and 10 U polynucleotide kinase (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... Phosphate incorporation was visualized by adding 1 µCi 32P-γ-ATP (200 cpm/pmol, Perkin Elmer), plus autoradiography or a PhosphorImager system (Molecular Dynamics) ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to different concentrations of purified YegI (0.0625,0.125,0.25,0.5,1,2 μM ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to 1 μM of purified YegI in 10 μl of reaction buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1/20th of the annealed substrates were 5′-end-labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: 1.5 μg RNA probes (5xAAAAUU or 5xGGGGCC) (Dharmacon) were end-radiolabelled with γ[32P]-ATP (PerkinElmer) using T4 polynucleotide kinase (PNK ...
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bioRxiv - Microbiology 2021Quote: ... in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol-Perkin Elmer # BLU002A). The labelled RNA was gel-purified as described above.
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bioRxiv - Molecular Biology 2020Quote: ATP luminescence was determined in triplicates using the ATPlite luminescence assay (Perkin Elmer, Waltham, MA, USA) with a ClarioStar Platereader (BMG ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... [3H]-α,β-methylene ATP concentrations were checked on Tri Carb scintillation beta counters (PerkinElmer, USA). Data analysis was conducted using GraphPad Prism v9 ...
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bioRxiv - Microbiology 2022Quote: ... Northern probes were 5’ 32P-end labeled with 0.3 mCi of y-32P ATP (Perkin Elmer) with 10 U of T4 Polynucleotide kinase (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the kinase was autophosphorylated for 1 h at 30 °C with [γ-32P]ATP (Perkin Elmer) before being combined with RRs at a 1:4 ratio (10-μl reactions contained 1 μM EnvZ and 4 μM RR) ...
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bioRxiv - Developmental Biology 2022Quote: ... then the signal was revealed using Tyramide Signal Amplification (TSA) with Fluorescein TSA Plus Evaluation Kit (Perkin Elmer, MA).
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bioRxiv - Genetics 2022Quote: ... Anti-Dig-peroxidase was applied onto hybridized slides and color was developed by Tyramide signal amplification (TSA) Fluorescein according to the manufacturer’s instructions (PerkinElmer). Cytokeratin 8 (Iowa hybridoma bank ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Slides underwent immunocytochemical processing in a humid chamber using the PerkinElmer TSA Fluorescein system (NEL701001KT; PerkinElmer, Waltham, MA, USA). Tissues were coated with blocking buffer for 30 minutes prior to anti-DIG-HRP conjugate for 2 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-Dig-peroxidase was applied onto hybridized slides and color was developed by Tyramide signal amplification (TSA) Fluorescein according to the manufacturer’s instructions (PerkinElmer).
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bioRxiv - Immunology 2022Quote: ... ISH was performed using the RNAScope Multiplex Fluorescent V2 Assay (Advanced Cell Diagnostics (ACD)) with TSA Plus fluorescein (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Bulk levels of ATP were measured using a luciferase-based biochemical assay (ATPlite Luminescence Assay System, PerkinElmer). Wing discs from third instar upcrawling larvae were dissected in culture medium ...
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bioRxiv - Genomics 2020Quote: ... at 37°C for 1 hr and then labeled with 0.5μl of gamma 32P-ATP (PerkinElmer, BLU502A250UC) using 20U T4 polynucleotide kinase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... 100 pmol of RNA were 5’-end labeled with [γ- 32P]-ATP (3000 Ci/mmol, Perkin Elmer) using T4 polynucleotide kinase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... and 2.5 μCi of [γ32P]-ATP (stock of 6000 Ci/mmol 10 mCi/mL, from Perkin Elmer). Autophosphorylation reactions were performed at 30 °C ...
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bioRxiv - Physiology 2022Quote: ... and 0.01% Triton X-100) in the presence of 10 μCi [32P]-γ-ATP (Perkin Elmer Company) with a final volume of 20 μl ...
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bioRxiv - Molecular Biology 2022Quote: DNA probes (U1-SH, U4-14B, and U6-SH) were end labeled with [γ-32P]-ATP (PerkinElmer) using T4 PNK (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Aptamers (15 pmol) and DNA double stranded oligonucleotides were end-labelled with [γ-32P] ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Biophysics 2021Quote: ... The primer was labeled on its 5’end with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol−1) and T4 polynucleotide kinase (Thermofisher) ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer), with U6 served as loading control ...
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bioRxiv - Biochemistry 2024Quote: Radiolabeled 32P-ATP (3000 Ci/mmol) used for synthesis of 32P-cGAMP was purchased from Perkin Elmer. 1 μM purified sscGAS was incubated with 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Radiolabeling was performed on the 5’ end of oligonucleotides using [γ-32P] ATP ([6000μCi/mmol] (Perkin Elmer) and polynucleotide kinase (Roche Applied Science ...
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bioRxiv - Microbiology 2024Quote: ... ATP production was measured by luminescence (according to manufacturer indications) in an Envision Multilabel Plate Reader (PerkinElmer) using a white opaque 384-plate (Ref ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was performed using the either the TSA Cyanine 3 Plus or the TSA Plus Fluorescein Evaluation Kits (Perkin Elmer).
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bioRxiv - Neuroscience 2024Quote: ... Fluorescent signals corresponding to the target transcript were generated using the Tyramide Signal Amplification (TSA) Plus Fluorescein Kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and incubated with TSA Plus Fluorescein (PerkinElmer) according to the manual at RT for 3-15 minutes (3-5 min in H ...
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bioRxiv - Immunology 2024Quote: ... the distribution of fluorescein isothiocyanate (FITC) dextran in the mice was observed via a small animal imaging system (IVIS Spectrum, PerkinElmer). The peripheral blood of the mice was collected and centrifuged at 3000 r/min ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT, 0.5 % Sarkosyl, 0.4 u/μl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Equal amounts of nuclei extracted from Drosophila S2 cells were used as spike-in material in run-on reactions ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling reactions were designed to use equimolar amounts of DNA and γ-32P-ATP (Perkin Elmer cat. # NEG035C001MC) and consisted of 37.5 pmol (7.5 μl of 5 μM ...
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bioRxiv - Microbiology 2021Quote: ... of which one was [5’-32P] single-end labelled with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol-1) and T4 polynucleotide kinase (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... ATP concentrations were then measured using 1.0 second luminescence exposure on a Wallac3 Victor plate reader (PerkinElmer, USA). For the live/dead assay ...