Labshake search
Citations for PerkinElmer :
201 - 250 of 431 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: Intracellular ATP level was measured using ATPlite Assay Kit (Perkin Elmer) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ATP production was measured according to the manufacturer’s instruction (PerkinElmer, 6016943). Briefly ...
-
bioRxiv - Biochemistry 2021Quote: ... 5mM ATP and 50uM (0.5 nCi) L-[14C]Glutamate (Perkin Elmer) in a buffer consisting of 30mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: The Adenosine TriPhosphate (ATP)-monitoring luminescence assay ATPLite™ (PerkinElmer, #6016943) was used to quantitatively assess proliferation and cytotoxicity of 3D MCF-7 cultures ...
-
bioRxiv - Molecular Biology 2021Quote: ... The probes were 5′ labelled using [γ-32P]-ATP (Perkin Elmer) and DECAprime™ II DNA labelling kit (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... The [g-32P]ATP (3,000 Ci/mmol) was supplied by PerkinElmer. T4 polynucleotide kinase (T4PNK ...
-
bioRxiv - Cell Biology 2023Quote: ... and 100µM 32P-ATP (specific activity: 1µCi per nmole, Perkin Elmer). Kinase assays were performed at 30 °C for 30 minutes and were terminated by addition of Laemmli sample buffer before SDS-PAGE and exposure to film ...
-
bioRxiv - Molecular Biology 2023Quote: ... in the presence of γ-32P-labeled ATP (PerkinElmer, Waltham, MA). Radiolabeled RNAs were gel-purified using denaturing PAGE as described above ...
-
bioRxiv - Biochemistry 2023Quote: ... Substrates were 5’ end-labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... T4 PNK was used to ligate γ-[32P] ATP (Perkin Elmer) to 5’ end of the RNA ...
-
bioRxiv - Biochemistry 2024Quote: ... ATP and 825nM ɑ-32P-UTP 3000 Ci/mmol (Perkin Elmer) for 1 hour at 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... ATP levels were determined using the ATPlite 1-step kit (PerkinElmer). Briefly ...
-
bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... RNA was detected using a fluorescein and a Cy3 Tyramide Amplificafion system (Perkin Elmer). Combination of fluorescent in situ hybridization with antibody staining was performed as follows ...
-
bioRxiv - Developmental Biology 2022Quote: ... tyramide signal amplification (TSA) -based method (TSA Plus Cy3 NEL744001KT /Fluorescein NEL741001KT; Perkin Elmer) was used for fluorescent detection and AP-based method for colorimetric detection.
-
bioRxiv - Neuroscience 2020Quote: ... avidin-biotinylated horseradish peroxidase kit (Vector) followed by fluorescein tyramide signal amplification (Perkin Elmer) were applied per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Probes were visualized using the TSA® Plus Fluorescein (1:1000, Perkin Elmer NEL741E001KT), TSA® Plus Cyanine 3 (1:1000 ...
-
bioRxiv - Developmental Biology 2020Quote: ... After detecting the first probe signal with TSA® fluorescein reagent (PerkinElmer; Waltham, MA) and several washes with TNT buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... before being stained with the PerkinElmer TSA Fluorescein system (NEL701001KT; PerkinElmer, Waltham, MA, USA). Slides were placed in a humid chamber and treated with blocking buffer followed by an anti-DIG-HRP conjugate for 2 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... Fluorescein-conjugated tyramide was diluted (1:150) in amplification buffer (Perkin Elmer, Boston, MA), and was applied in the dark for 5–6 min on ice.
-
bioRxiv - Neuroscience 2023Quote: ... the signal was detected by the TSATM Plus Cyanine 3 / Fluorescein System (PerkinElmer, NEL753001KT).
-
The Iroquois (Iro/Irx) homeobox genes are conserved Hox targets involved in motor neuron developmentbioRxiv - Developmental Biology 2024Quote: ... RNA was detected using a fluorescein and a Cy3 Tyramide Amplificafion system (Perkin Elmer).
-
bioRxiv - Molecular Biology 2020Quote: ... Ten nanograms of crRNA was 5′-labeled with [γ-32P]-ATP (PerkinElmer) using T4 polynucleotide kinase (New England Biolabs ...
-
bioRxiv - Biochemistry 2020Quote: γ[32P]-ATP and α[32P]-dCTP were purchased from PerkinElmer (Waltham, MA). Unlabelled ATP was purchased from DOT Scientific (Burton ...
-
bioRxiv - Biochemistry 2020Quote: ... 26nt synthetic RNA oligo (IDT) labelled with [γ-P32]ATP (PerkinElmer, BLU502A250UC) as described above was added into lysate fractions to final concentration 1μM ...
-
bioRxiv - Biochemistry 2020Quote: ... Reaction was started by adding 3.7 kBq of [γ-32P]ATP (PerkinElmer) in a total reaction volume of 10 μL ...
-
bioRxiv - Neuroscience 2020Quote: ... The RNA was then 5’-end-labeled with γ-(32P) ATP (PerkinElmer) using T4 polynucleotide kinase (New England Biolabs ...
-
bioRxiv - Biochemistry 2021Quote: ... The oligos were 5’-end radiolabeled with gamma-[32P]-ATP (Perkin Elmer) using T4 polynuclotide kinase (NEB ...
-
bioRxiv - Microbiology 2021Quote: ... ATP measurement was performed using the ATPlite Luminescence Assay System (6016941, PerkinElmer) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2020Quote: ... 3.5 μl ATP content reagent mixture (Catalog No. 6016941, PerkinElmer, Waltham, MA) was added to each well in the assay plates and incubated for 30 mins at RT ...
-
bioRxiv - Immunology 2022Quote: Intracellular ATP levels were measured with the ATPlite Luminescence Assay System (PerkinElmer) following the vendor protocol ...
-
bioRxiv - Immunology 2020Quote: ... ATP was measured using the ATPlite luminescence assay system (PerkinElmer, Waltham, MA). One hour treatments were determined to be sufficient for efficient ATP removal and thus were used in neuron stimulation assays.
-
bioRxiv - Biochemistry 2020Quote: ... The RNA was subsequently 5’-end labeled with [γ32-P] ATP (PerkinElmer) using T4 polynucleotide kinase (PNK ...
-
bioRxiv - Biochemistry 2020Quote: ... Oligonucleotides were radioactively [5’-32P]-labelled using [γ-32P] ATP (Perkin Elmer) using T4 polynucleotide kinase (Fermentas ...
-
bioRxiv - Biochemistry 2021Quote: The 3xTEL oligo was 5⍰ radiolabeled with [γ-32P]ATP (NEG035C005MC, PerkinElmer) using a standard T4 polynucleotide kinase labeling protocol (M0201L ...
-
bioRxiv - Cell Biology 2022Quote: ... and then radiolabeled with 0.5 μCi (γ-32P)ATP (Perkin Elmer NEG002Z250UC) using T4 PNK kinase (NEB M0201L ...
-
bioRxiv - Molecular Biology 2024Quote: ... with [γ-32P] ATP (10mCi/mL, 3,000 Ci/mmol; Perkin Elmer, BLU502A250UC) in 1× CutSmart buffer supplied with DTT to 5 mM ...
-
bioRxiv - Molecular Biology 2023Quote: ... ATP content in these samples was determined with ATPlite 1step (PerkinElmer ®). The OXPHOS yield is defined by the ratio of ATP production rate per oxygen consumption rate ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 0.23 μl of [γ-32P] ATP (6000 Ci/mmol, Perkin Elmer). Twenty percent of the reaction mixture was spotted onto a polyethyleneimine cellulose plate (MACHEREY-NAGEL ...
-
bioRxiv - Biochemistry 2024Quote: ... The oligonucleotides were 5’-end labelled with [γ-32P]-ATP (PerkinElmer, France) or labelled with fluorescent dye Cy5 (Cyanine5 ...
-
bioRxiv - Cell Biology 2024Quote: ... Viability was determined by measurement of ATP using ATPlite assay (Perkin Elmer). Dose-response curves were generated and fitted with IC50 values using GraphPad Prism (dose-response with variable slope model).
-
bioRxiv - Plant Biology 2024Quote: ... 5 pmol of dephosphorylated RNAs were labeled with 32P-γ-ATP (PerkinElmer) by T4 Polynucleotide Kinase (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2024Quote: ... DNA oligos (Table S1) were radiolabeled with [γ-32P] ATP (Perkin Elmer) by T4 polynucleotide kinase (New England Biolabs ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5mM DTT) with or without 10μCi of ATP-γ-32P (PerkinElmer NEG002A250UC). Reactions were incubated at 30⁰C for 30 minutes and terminated with loading dye (Biorad #161074) ...
-
bioRxiv - Developmental Biology 2020Quote: ... After detection of digoxigenin-labeled probes by deposition of TSA Plus fluorescein solution (Perkin Elmer), embryos were incubated with an AlexaFluor 647 goat anti-mouse IgG secondary antibody (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated with an anti-fluorescein horseradish peroxidase conjugate (1:500, PerkinElmer, Waltham, MA, USA) overnight at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Color was developed by TSA (Tyramide signal amplification) Fluorescein according to the manufacturer’s instructions (PerkinElmer). Nuclear staining was performed using Hoechst 33342 (4 μg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections were incubated with Tyramide-conjugated Cy3 or fluorescein (1:75, PerkinElmer, NEL744001KT, NEL741001KT) at room temperature for 20 min to visualize the in situ signals ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... a given histidine kinase was first autophosphorylated with [γ-32P]-ATP (Perkin Elmer) for 90 minutes at 30 °C to drive autophosphorylation and then mixed at a 1:4 molar ratio with a response regulator (1 μM EnvZ ...
-
bioRxiv - Microbiology 2020Quote: ... Reactions were started by adding 10 μCi of [γ-32P]ATP (PerkinElmer, BLU002A500UC), and 10 μl samples were removed at different times ...