Labshake search
Citations for PerkinElmer :
151 - 200 of 205 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Prior to sequencing libraries were prepared using NEXTFLEX Rapid DNA-Seq Library Prep Kit for Illumina Platforms (PerkinElmer, Waltham, MA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was sequenced on both strands by primer walking using the ABI PRISMDye Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer) and an ABI PRISM 377 sequencer (Perkin-Elmer ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA is extracted using magnetic bead-based chemistry in conjunction with the Chemagic MSM I instrument manufactured by Perkin Elmer. Following red blood cell lysis ...
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bioRxiv - Physiology 2020Quote: ... as per manufacturer’s instructions and loaded onto the DNA 5K/RNA/Charge Variant Assay LabChip (Perkin Elmer, Cat. No. 760435) to be read using a LabChip GX Touch Nucleic Acid Analyser (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA extraction from K2-EDTA-blood tubes was performed with chemagic 360 instrument and the CMG-704 kit (PerkinElmer), which uses magnetic bead technology ...
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bioRxiv - Molecular Biology 2022Quote: ... The reverse primer contained an Illumina 3’ adapter sequence and an index sequence to uniquely identify each library (NextFlex DNA barcodes, PerkinElmer). Libraries were purified and quantified using the dsDNA Fluorescence Quantification Assays (DeNovix ...
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... DNA quantification and quality check was done using a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA, USA). Libraries were prepared as detailed on the Illumina® support website (http://support.illumina.com ...
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bioRxiv - Microbiology 2022Quote: ... Quality of the libraries was measured with LabChip GX Touch HT DNA High Sensitivity Reagent Kit (PerkinElmer, Waltham, MA, USA). Sequencing was performed with NovaSeq 6000 System (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Molecular Biology 2023Quote: ... reagents and conditions of the BigDye Terminator v3.1 Cycle sequencing Kit and an automated capillary sequencer ABI 3700 DNA analyzer (Perkin Elmer, Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... blood was collected in EDTA anti-coagulant BD Vacutainer tubes and genomic DNA was extracted using the Chemagic MSM I Instrument (PerkinElmer) following the manufacturer’s recommendations and stored at −20°C before PCR 44 ...
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bioRxiv - Plant Biology 2023Quote: ... Purified RNA was quality checked for DNA contamination and integrity using semi-denaturing agarose-gel electrophoresis and the LabChip GX Touch (PerkinElmer). RNA was quantified with ND-1000 Spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Samples were mixed with the DNA absorption solution (for one sample: 50 uL 5 M NaCl, 15 ul magnetic beads (GMG-252-A-100mL – PerkinElmer), 250 uL 7.8 M guanidium chloride ...
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bioRxiv - Plant Biology 2024Quote: ... the fragmented gDNA was constructed into sequencing libraries using a NEXTFLEX Rapid DNA Seq Kit (PerkinElmer, Inc., Austin, TX, USA). The full-length coding sequence of ZmHsf20 was cloned into the pFN19K HaloTag T7 SP6 Flexi vector ...
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bioRxiv - Evolutionary Biology 2023Quote: Libraries were prepared from 33ng of genomic DNA using a custom miniaturized version of the NEXTFLEX Rapid XP kit protocol (PerkinElmer) that was automated on a Sciclone NGSx liquid handler (PerkinElmer) ...
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bioRxiv - Neuroscience 2021Quote: ... Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol; Perkin Elmer) using the MEGAshortscript T7 kit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: 10 pmol of single-stranded DNA (Eurofins MWG Operon, Germany) were labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) by incubation with terminal deoxynucleotidyl transferase (TdT ...
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bioRxiv - Biochemistry 2020Quote: 10 pmol of single-stranded RNA or DNA (Eurofins MWG Operon, Germany) was incubated with 6.8 pmol γ-32P-dATP (Perkin Elmer), and 10 U T4 PNK (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Final library products were assessed for quality on LabChip GX using the DNA High Sensitivity Reagent Kit (Perkin Elmer, MA, USA), diluted to 2 nM concentration and pooled ...
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bioRxiv - Microbiology 2020Quote: ... Stool samples underwent DNA extraction using chemical and mechanical lysis with magnetic bead-based purification via the Chemagic MSM I with the Chemagic DNA Blood Kit-96 from Perkin Elmer. Prior to extraction on the MSM-I ...
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bioRxiv - Genomics 2021Quote: Plate-based DNA library preparation for Illumina sequencing was performed on the Sciclone NGS robotic liquid handling system (PerkinElmer, Waltham, USA) using KAPA Biosystems library preparation kit (Roche ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Microbiology 2019Quote: ... 800 uL of supernatant was then transferred to a deep well block and DNA extracted and purified using a Chemagic MSM I (Perkin Elmer) following the manufacturers protocol ...
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bioRxiv - Genomics 2019Quote: ... DNA libraries were made using the Illumina TruSeq Nano DNA HT kit and their size and concentration determined using LabChip DX Touch (Perkin Elmer) and Quant-iT (Life Technologies) ...
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bioRxiv - Biochemistry 2020Quote: ... The 500 nM annealed DNA substrates were then radiolabeled in PNK buffer with PNK enzyme and radioactive ATP [γ32P] from Perkin Elmer at 37°C for 1 hr then diluted in TE buffer (10 mM Tris ...
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bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42 °C with 4 pmol DNA probes radiolabeled with [γ-32P]ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA from parents and progenies was extracted from 100 mg of frozen young leaves with the Chemagic DNA Plant Kit (Perkin Elmer Chemagen ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios ...
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bioRxiv - Molecular Biology 2022Quote: ... and a DNA loading control also terminating in 20 adenosines (IDT) (Supplementary Table S2) were 5’ labelled with [γ-32P]-ATP (PerkinElmer) using T4 poly-nucleotide kinase (NEB ...
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bioRxiv - Immunology 2023Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer). The resulting libraries were sequenced on a multiplexed paired end 100bp Illumina NovaSeq 6000 S4 flowcell ...
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bioRxiv - Biochemistry 2023Quote: ... 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Developmental Biology 2021Quote: ... M13mp18 ssDNA was added as a template for DNA replication at the indicated concentrations in presence of α-;32PdCTP (3000Ci mmol−1, Perkin Elmer). At the indicated time points half of the samples were neutralized in 10 mM EDTA ...
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bioRxiv - Genomics 2019Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX (Perkin Elmer, Waltham, MA). Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
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bioRxiv - Cancer Biology 2020Quote: ... The molar concentration of cDNA molecules in the individual libraries was calculated from the double-stranded DNA concentration and the single library average size (determined on a PerkinElmer Labchip GX). An equal number of cDNA molecules from each library were pooled and the final pool was purified once more in order to remove any free primer and prevent index-hopping ...
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bioRxiv - Cell Biology 2022Quote: ... The molar concentration of cDNA molecules in the individual libraries was calculated from the double-stranded DNA concentration and the single library average size (determined on a PerkinElmer Labchip GX). An equal number of cDNA molecules from each library were pooled and the final pool was purified once more in order to remove any free primer and prevent index-hopping ...
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bioRxiv - Molecular Biology 2020Quote: 100 pmol of WT or mutant RNA oligonucleotides (synthesized by Integrated DNA Technologies, Inc.) were 5’- end labeled with [γ32P] ATP (Perkin Elmer, NEG035C001MC) and T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... the R2R and primer extension (PE) DNA primers (100 pmol) were labeled with [γ-32P]ATP (125 pmol; 6,000 Ci/mmol; 150 μCi/ μl; PerkinElmer Life Sciences) by incubating the DNA with T4 polynucleotide kinase (10 units ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from 250 mg of leaves ground to powder under liquid nitrogen using the Chemagic DNA Plant Kit (Perkin Elmer, Germany), according to the manufacturer’s instructions on a KingFisher Flex™ (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... 50 U of DNA polymerase I (M0209S) and 30 μM of each dNTP including 6 μM of Fluorescein-12-dATP (Perkin Elmer, NEL465001EA) or 6 μM of Texas Red-5-dATP (Perkin Elmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood samples or buffy coat extracts were extracted in Auria Biobank for all patients for DNA extraction and genomic sequencing to serve as germline reference for identifying somatic genomic aberrations using Chemagic DNA Blood Kit Special (PerkinElmer Inc., USA) and Chemagic 360 instrument (PerkinElmer Inc. ...
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bioRxiv - Microbiology 2021Quote: ... The volumes of internalised DNA in GFP expressing bacteria were estimated by 3-D image analysis performed using Volocity software (Perkin Elmer, Waltham, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... were added to the PCR product and sequencing was conducted using the automated DNA Sequencer (ABI 3130xl Genetic Analyzer; Perkin Elmer/Applied Biosystems). Using the software Genemapper ver ...
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bioRxiv - Immunology 2020Quote: ... The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer, Waltham, MA, USA). The libraries were qPCR quantified (Kapa Biosystems ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Extracted DNA was sent to Texas A&M Agrilife Bioinformatics and Genomics Service (College Station, TX, USA) for whole-genome library preparation using a NEXTFLEX Rapid XP DNA-Seq Kit HT (PerkinElmer, Waltham, MA, USA). Libraries were sequenced at the Texas A&M Agrilife Bioinformatics and Genomics Service on one NovaSeq 6000 S4 lane at 26.1 ± 5.8 depth of coverage per sample across the full genome (2 s.d. ...
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bioRxiv - Molecular Biology 2022Quote: U10 and CUGCUGUUUU (20 pmol) were chemically synthesized (Integrated DNA Technologies (IDT)) and 20 pmol RNA was radioactively labeled using [γ-32P]-ATP (PerkinElmer, 10mCi/ml) and 10 units T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer, Waltham, MA). The library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...