Labshake search
Citations for PerkinElmer :
101 - 150 of 205 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were made with the ChIPed-DNA using Nextflex ChIP-Seq Library Prep kit (PerkinElmer). Sequencing reading was performed with a NovaSeq 6000 system (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA or RNA oligonucleotides were 5′ radiolabeled with [γ-32P]ATP (PerkinElmer, 3000 Ci/mmol), using T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... and the NEXTFLEXTM Rapid DNA-seq Kit (NOVA-5114-03 and NOVA-5144-04; PerkinElmer) was used to prepare 10-50 ng of cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Viral RNA was extracted using the chemagic™ Prime Viral DNA/RNA 300 Kit (PerkinElmer) on a Chemagic Prime 8 system (PerkinElmer) ...
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bioRxiv - Microbiology 2020Quote: ... 100 pmol of DNA was incubated with 2 μL 5mCi [γ32-P]ATP (Perkin Elmer) and 4 μL T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were constructed using the NEXTFLEX Rapid DNA-Seq Kit (PerkinElmer, Inc., Austin, TX, USA) according to the instruction manual ...
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bioRxiv - Biochemistry 2023Quote: ... 100 pmol of DNA was incubated with 2 μL 5 mCi [γ32-P]ATP (PerkinElmer) and 4 μL T4 PNK (New England BioLabs ...
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bioRxiv - Genomics 2019Quote: ... The DNA fragments and libraries were characterized using a Labchip GX Touch HS Kit (Perkin Elmer) or TapeStation (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Cancer Biology 2020Quote: ... checked with Caliper LabChip GX for DNA samples using the High Sensitivity Assay (PerkinElmer, Inc. USA) and quantified with the Quant-iT dsDNA HS Assay Kit using Qubit fluorometer (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Synthesis of nascent DNA was then measured by quantification of radiolabelled α32P-dATP (NEG512H250UC, Perkin Elmer) incorporation into newly synthesised DNA ...
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bioRxiv - Immunology 2020Quote: ... ChIP DNA libraries were ligated with the Bioo scientific barcoded adaptors (BIOO Scientific, Perkin Elmer, USA) with T4 DNA ligase according to KAPA LTP library preparation protocol and the ligated ChIP DNA libraries were purified with 1.8x vol ...
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Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteriabioRxiv - Cell Biology 2019Quote: ... The final DNA concentrations were determined using an ultraviolet-visible (UV-VIS) spectrophotometer (PerkinElmer Lambda 950) and the DNA molecular mass was determined by gel electrophoresis with 1% agarose ...
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bioRxiv - Genomics 2023Quote: ... we purified DNA from 15 mg of dried leaves of each sample with Chemagic beads (PerkinElmer) following Holtz et al ...
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bioRxiv - Developmental Biology 2023Quote: ... The annealed synthetic oligonucleotide DNA was labeled with [α-32P]dCTP (Perkin Elmer NEG013H250UC, 3000Ci/mmol) by Klenow polymerase ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1 μg of plasmid DNA and 20 μCi of Easy-tag 35S-methionine (Perkin Elmer) were mixed with a final volume of 50 μl ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was subjected to 40 cycles of amplification using a DNA thermal cycler 2720 (Perkin Elmer). For duplex PCR of the HPV L1 gene and human beta-actin gene ...
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bioRxiv - Molecular Biology 2022Quote: DNA probes (U1-SH, U4-14B, and U6-SH) were end labeled with [γ-32P]-ATP (PerkinElmer) using T4 PNK (Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Single-tethered DNA was stretched with blocking buffer containing 1 µg/mL biotinylated anti-digoxigenin (Perkin Elmer) at 100 µL/min flow rate ...
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bioRxiv - Immunology 2020Quote: ... The amount of radiolabeled DNA was measured on a 1450 MicroBeta Trilux Microplate Scintillation counter (Perkin Elmer). The experiments were carried out at least three times.
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bioRxiv - Molecular Biology 2020Quote: Aptamers (15 pmol) and DNA double stranded oligonucleotides were end-labelled with [γ-32P] ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Immunology 2020Quote: ... The amount of radiolabeled DNA was measured on a 1450 MicroBeta Trilux Microplate Scintillation counter (Perkin Elmer). The experiment was carried out three times.
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA probes were prepared by end-point PCR and labeled with dCTP [α-32P] (PerkinElmer) using the Cytvia Amersham Megaprimer labeling kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... DNA methylation was measured by the incorporation of tritiated methyl groups from radioactively labeled SAM (Perkin Elmer) into the biotinylated substrate using an avidin–biotin methylation plate assay47 ...
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bioRxiv - Genetics 2024Quote: Genomic DNA of blood donors was extracted on a Chemagic MSM I extraction robot (Chemagen, Perkin Elmer). High-throughput genotyping of 46 blood group antigens of 35,954 donor samples collected in the years 2015 to 2021 was performed by MALDI-TOF MS as described previously [16,17] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with 15 cycles of PCR and using 1:125 dilution of NEXTflex DNA barcodes (Perkin Elmer,NOVA-514104). The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling reactions were designed to use equimolar amounts of DNA and γ-32P-ATP (Perkin Elmer cat. # NEG035C001MC) and consisted of 37.5 pmol (7.5 μl of 5 μM ...
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bioRxiv - Neuroscience 2019Quote: FAAH and CB1 DNA linearized for antisense was mixed with 35S-conjugated to uracil and adenine (Perkin Elmer) incubated at 37°C for 1.5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... DNA samples and blank were measured in duplicate using EnSpire® Multimode Plate Reader (PerkinElmer Inc, Waltham, USA). By plotting the measured fluorescence versus DNA ...
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bioRxiv - Genomics 2020Quote: DNA from saliva collected with mouthwash samples was extracted on a chemagic platform (PerkinElmer chemagen Technologie GmbH, Germany). DNA collected with Oragene OG500 Kits (DNA Genotek Inc. ...
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bioRxiv - Genetics 2020Quote: ... and fragmented DNA was ligated to index adapters and amplified (BioO NEXTflex qRNA-Seq Kit v2, Perkin Elmer). Indexed DNAs smaller than 500 bp were gel purified and subjected to next generation sequencing (HiSeq 4000 ...
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bioRxiv - Genomics 2022Quote: ... was used for NGS library construction using a NEXTflex Rapid DNA-Seq Kit 2.0 (PerkinElmer, Inc., Waltham, MA). Exome regions were enriched via hybridization with an IDTexome panel (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNAs were detected with DNA oligonucleotides (Table S2) end-labelled with [32P] γ-adenosine-triphosphate (Perkin Elmer) using T4 polynucleotide kinase (Roche) ...
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bioRxiv - Immunology 2023Quote: ... Libraries were QC’ed using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer, Waltham, MA). Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
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bioRxiv - Genetics 2021Quote: ... DNA fragments were detected using a mix of five probes labelled by PCR amplification with 32P dCTP (Perkin Elmer) described in Fig S1 ...
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Neuroscience 2019Quote: ... and the library quality was estimated by utilizing a DNA High Sense chip on a Caliper Gx (Perkin Elmer). Accurate quantification of the final libraries for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification kit (Kapa Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Pooled samples were size selected with the LabChip XT (LabChip XT DNA 300 Assay Kit; PerkinElmer, Seer Green, UK) and diluted to 26 pM for templating with the Ion OneTouch 2 system using the Ion PGM Template OT2 200 kit ...
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bioRxiv - Immunology 2021Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... A fragmentation step was carried out for 6.5 minutes at 94°C and 7 μM NextFlex DNA barcodes (PerkinElmer) were used for adapter ligation ...
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bioRxiv - Plant Biology 2022Quote: ... Library insert sizes was confirmed by running an aliquot of the DNA library on a PerkinElmer GX (PerkinElmer CLS760672) and concentration measured using the Tecan plate reader.
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bioRxiv - Pathology 2023Quote: ... Supernatant was discarded and DNA was extracted using the QuicPickTM SML Plant DNA purification kit according to the supplier’s instructions (Bio-Nobile, Turku, Finland) in an automated system (Caliper Zephyr, PerkinElmer). DNA was eluted from magnetic beads using 30 µL of elution buffer ...
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bioRxiv - Genomics 2023Quote: ... the converted DNA was then used for the library preparation using the NEXTFLEX® Bisulfite-Seq kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and the library quality was estimated by utilizing a DNA High Sense chip on a Caliper Gx (Perkin Elmer). Accurate quantification of the final libraries for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC, PerkinElmer) for 1 h at 30 °C at 600 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... which were incubated with various concentration of synthetic biotinylated TAR-RNA (biotin 5’-dT/GGU CUC UCU GGU UAG ACC AGA UCU GAG CCU GGG AGC UCU CUG GCU AAC UAG GGA ACC/3’-biotin dT) (Integrated DNA Technologies) at 4°C for 3 h in the AlphaLISA buffer (PerkinElmer). Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer). Libraries were pooled in equimolar ratios ...
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bioRxiv - Molecular Biology 2019Quote: ... All DNA extractions were performed using the QuickPickTM SML Plant DNA Kit (Bio-Nobile, Turku, Finland) as in PM7/24 (EPPO, 2018b) with an automated system (Caliper Zephyr, PerkinElmer). A control composed of DNAs extracted from bacterial suspensions were systematically performed.