Labshake search
Citations for PerkinElmer :
1 - 50 of 205 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... DNA FISH measurements were made using the quantitation module of Volocity (PerkinElmer) and only alleles with single probe signals were analysed to eliminate the possibility of measuring sister chromatids.
-
bioRxiv - Genetics 2021Quote: ... DNA FISH measurements were carried out using the quantitation module of Volocity (PerkinElmer Inc, Waltham MA). For DNA FISH ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... HeLa cell painting and Sperm viability (PerkinElmer 384-well PhenoPlate), Acrosome (Greiner 384-PP V-bottom plates).
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescein (Fluorescein Direct FISH; PerkinElmer Life Sciences, Waltham, MA) was used to visualize labeled cells ...
-
bioRxiv - Neuroscience 2023Quote: ... Fluorescein (Fluorescein Direct FISH; PerkinElmer, REF# 14921915, Lot #: 2587974) and Cy3 (TSA Cyanine 3 Tyramide ...
-
bioRxiv - Neuroscience 2022Quote: ... Cyanine-3 (Cy3 Direct FISH; PerkinElmer Life Sciences, Waltham, MA) was used to visualize labeled cells and nuclei were counterstained with DAPI (ThermoFisher Scientific).
-
bioRxiv - Cell Biology 2020Quote: ... FISH signal was labeled with TSA Plus Cyanine 5 (Perkin Elmer). Immunocytochemistry was carried out after FISH procedure ...
-
bioRxiv - Neuroscience 2019Quote: ... FISH was visualized using the Tyramide In Situ System (Perkin Elmer) as previously described [4] ...
-
bioRxiv - Neuroscience 2022Quote: RNA FISH (based upon the Tyramide Signal Amplification™ method (Perkin Elmer)) and protein immunofluorescence on whole mount antennae or antennal cryosections were performed essentially as described (Saina and Benton ...
-
bioRxiv - Neuroscience 2024Quote: ... Dual RNA-FISH and immunocytochemistry images were analysed using Columbus 2.8 (PerkinElmer): RNA FISH load was determined by calculating the integrated intensity of nuclear RNA puncta by multiplying spot intensity by total spot load per CasRx positive cell (as determined by nuclear HA positivity) ...
-
bioRxiv - Neuroscience 2021Quote: ... and FISH signals were detected using a TSA Plus Cyanine 3 kit (NEL744001KT, PerkinElmer). To visualized the GFP signals ...
-
bioRxiv - Developmental Biology 2021Quote: ... FISH was performed first using the TSA plus Cyanine 3 kit (Perkin Elmer NEL744001KT), followed by IHC with anti-GFP primary antibody (abcam290 ...
-
bioRxiv - Genomics 2023Quote: ... RNA FISH signal quantification was carried out using the quantitation module of Volocity (PerkinElmer). Number of expressing alleles were calculated by segmenting the hybridization signals and scoring each nucleus as containing 0 ...
-
bioRxiv - Genetics 2021Quote: ... Waltham MA).RNA FISH signal quantification was carried out using the quantitation module of Volocity (PerkinElmer Inc, Waltham MA). Expressing alleles were calculated by segmenting the hybridisation signals and scoring each nuclei as containing 0 ...
-
bioRxiv - Neuroscience 2019Quote: ... TSA-FISH slides were incubated in working solution containing Cy5 Plus tyramide according to manufacturer’s instructions (Perkin Elmer). Slides were then washed in NTT and incubated in 3%H2O2 for 30min to quench the first tyramide reaction ...
-
bioRxiv - Cell Biology 2021Quote: ... Single-color FISH was developed by a TSA-Plus TMR System according to the manufacturer⍰s protocol (PerkinElmer) using a digoxigenin-labeled probe and an anti-dig-HRP antibody (Roche Applied Science) ...
-
bioRxiv - Physiology 2020Quote: ... sperm was exposed to exosomes or drugs and fluorescence intensity was assessed by the EnSpire® Multimode Plate Reader (PerkinElmer, Waltham, MA, USA) with excitation and emission wavelengths of 488 and 525 nm ...
-
bioRxiv - Genomics 2019Quote: ... FISH experiments were performed using human fosmids containing non-duplicated region directly labeled by nick-translation with Cy3-dUTP (PerkinElmer), Cy5-dUTP (PerkinElmer) ...
-
bioRxiv - Cell Biology 2020Quote: Section in situ hybridization and fluorescent in situ hybridization (FISH) with the tyramide signal amplification (TSA) Plus DNP system (PerkinElmer) were performed according to the procedure reported previously (Takei et al. ...
-
Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Fish were imaged first using SLR cameras and then were imaged using Quantum GX microCT imaging system (Perkin Elmer, USA). Image acquisition settings were as follows ...
-
bioRxiv - Neuroscience 2024Quote: RNA-FISH and immunocytochemistry experiments were all imaged using the automated Opera Phenix TM high-throughput confocal imaging platform (Perkin Elmer). Dual RNA-FISH and immunocytochemistry images were analysed using Columbus 2.8 (PerkinElmer) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and DNA High Sensitivity DNA reagents (PerkinElmer CLS760672). Equimolar quantities of each library were pooled ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted with the Chemagic DNA Plant Kit (Perkin Elmer Chemagen ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted using the Chemagic360 DNA tissue extraction kit (PerkinElmer, CMG-723) on a Chemagic360 nucleic acid extractor (PerkinElmer ...
-
bioRxiv - Microbiology 2019Quote: ... bacteria DNA was extracted using the chemagic STAR DNA BTS Kit for the automated isolation of DNA (Perkin Elmer, Wellesley, MA, USA). Quality and purity of the isolated DNA were checked by spectrophotometry on the NanoDrop (Fisher Scientific ...
-
bioRxiv - Physiology 2020Quote: ... 1 ng of eluted and purified DNA was used to prepare DNA sequencing library with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, NOVA-5188-01) on the NextSeq 500 system (Illumina ...
-
bioRxiv - Genomics 2023Quote: DNA was extracted using the Chemagic DNA Blood 2k Kit (Perkin Elmer CMG-1097) on a Chemagic 360 automated nucleic extraction instrument (Perkin Elmer ...
-
bioRxiv - Biochemistry 2023Quote: Microbial DNA was extracted using a DNA Stool 200 Kit special H96 (PerkinElmer, Turku, Finland) kit with a corresponding Chemagic with Magnetic Separation Module I (MSM I ...
-
bioRxiv - Molecular Biology 2021Quote: ... The beads were purified for DNA library construction with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
-
bioRxiv - Neuroscience 2021Quote: ... and NEXTFLEX DNA Barcodes (Perkin Elmer), with size selection performed with Agencourt AMPure XP (Beckman Coulter) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified DNA was used to construct a library with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
-
bioRxiv - Cancer Biology 2021Quote: ... DNA quality was assessed with Caliper LabChip GX for DNA samples using the High Sensitivity Assay (PerkinElmer, Inc. USA) and quantified using a Quant-iT dsDNA High Sensitivity Assay Kit on a Qubit fluorometer (Invitrogen ...
-
bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was isolated from a single colony using a JANUS Chemagic Workstation and Chemagic Viral DNA/RNA kit (PerkinElmer). Libraries were prepared with the Nextera XT DNA sample preparation kit (Illumina) ...
-
bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
-
bioRxiv - Developmental Biology 2022Quote: ... the NEXTFLEX Rapid XP DNA-Seq kit (PerkinElmer) and xGen Lockdown probes (Integrated DNA Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... and DNA High Sensitivity Reagent Kit (CLS760672, PerkinElmer). Libraries with unique dual indexes were pooled and sequenced on partial lanes targeting ∼50M reads per sample on a HiSeq or a NovaSeq 6000 sequencer (Illumina ...
-
bioRxiv - Cell Biology 2023Quote: ... using DNA High Sensitivity Reagent Kit (PerkinElmer: CLS760672) and LabChip GX Touch 24 (PerkinElmer ...
-
bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
-
bioRxiv - Systems Biology 2019Quote: ... The insert size of the libraries was verified by running an aliquot of the DNA library on a PerkinElmer GX using the High Sensitivity DNA chip (PerkinElmer CLS760672) and the concentration was determined by using a High Sensitivity Qubit assay and qPCR ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
-
bioRxiv - Molecular Biology 2020Quote: ... Sonicated DNA was end repaired followed by ligation of adapters to DNA overnight using NEXTFLEX Bisulfite-Seq Barcodes (PerkinElmer, Waltham, MA, USA). DNA was treated with TET2 for one hour ...
-
bioRxiv - Systems Biology 2019Quote: ... The insert size of the libraries was verified by running an aliquot of the DNA library on a PerkinElmer GX using the High Sensitivity DNA chip (Perkin Elmer, CLS760672) and the concentration was determined by using a High Sensitivity Qubit assay and q-PCR ...
-
bioRxiv - Plant Biology 2022Quote: ... and the eluted DNA quality was assessed with Caliper LabChip GX for DNA samples using the High Sensitivity Assay (PerkinElmer, Inc. USA) and quantified using a Quant-iT dsDNA High Sensitivity Assay Kit on a Qubit fluorometer (Invitrogen ...
-
bioRxiv - Genomics 2021Quote: ... DNA was extracted using the Chemagen kit (Perkin Elmer), in batches by cohort ...
-
bioRxiv - Microbiology 2022Quote: ... DNA probes were radiolabeled with [γ-32P]-ATP (PerkinElmer) using the T4-polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and DNA 5K/RNA/CZE HT Chip (760435, PerkinElmer) on a LabChip GX Touch HT Nucleic Acid Analyzer (CLS137031 ...
-
bioRxiv - Plant Biology 2023Quote: ... and LabChip GX-Touch (DNA High Sensitivity kit, PerkinElmer), respectively ...
-
bioRxiv - Genomics 2020Quote: ... and controlled the size of the amplicons on the GX Touch using the 3K kit (X-Mark DNA LabChip, CLS144006, HT DNA NGS 3K Reagent Kit, Perkin Elmer LAS (UK) LTD) ...
-
bioRxiv - Genomics 2022Quote: ... 1 ng of eluted and purified DNA was used to prepare libraries with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, #NOVA-5188-01) on the Illumina NextSeq500 system using a single read 100 bp protocol ...
-
bioRxiv - Genetics 2023