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Citations for PerkinElmer :
1651 - 1700 of 1735 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... We verified that these cells did not lose viability after overnight culture in serum-free medium relative to medium with 10% serum as determined by cell-based measurements of CBG bioluminescence in an IVIS Lumina (Perkin Elmer). We injected 1 × 105 breast cancer cells per mouse (n = 5 per each cell type) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then acquired a bioluminescence image of total viable cells on an IVIS Lumina (30 second image, large field of view) (Perkin Elmer). A person blinded to experimental conditions enumerated colonies and quantified imaging data (Living Image ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded at 200 cells per well in 40 μL in the inner-312 wells of 384-well white-walled culture plates (Perkin Elmer) and allowed to attach for 24-hours prior to drug treatment ...
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bioRxiv - Cancer Biology 2023Quote: For 2D adherent studies cells were seeded at 500-1,000 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 48 hours prior to drug treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at 500 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 24 hours prior to drug treatment ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice and cell-associated activity was subsequently measured in a well-type gamma-counter (Wallac 2480 wizard, Perkin Elmer). In a separate binding assay equimolar concentrations (1.97 pM ...
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bioRxiv - Biochemistry 2023Quote: ... Binding reactions were incubated for 2 hours and then terminated by washing the solutions over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by wash steps using ice-cold buffer [50 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The lysed cells were transferred to vials and measured in a gamma counter (Wallac 1470 Wizard, Perkin Elmer, Waltham, MA, USA). Obtained cpm values were plotted with GraphPad Prism 7 and the data were fitted using nonlinear regression (one site - fit Ki ...
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bioRxiv - Cell Biology 2023Quote: MIN6 cells were seeded at a density of 15,000 per well in a 384-well plate (Perkin Elmer, CellCarrier Ultra, #6057300). After 24 h recovery ...
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bioRxiv - Immunology 2023Quote: ... cells were isolated ex-vivo and then incubated for six hours with 0.5 μCi 14C-palmitic acid (Perkin Elmer Cat# NEC075H050UC) in the following media ...
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bioRxiv - Cancer Biology 2023Quote: ... and 25μL was added to the transfected cells, after which the baseline luminescence was measured for 15min (continuous mode, 1min intervals) using an Envision (Perkin Elmer) spectrophotometer ...
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bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Biochemistry 2023Quote: ... half the medium was removed from all wells and cells were lysed by the addition of 50 µl of BriteLite assay readout solution (Perkin Elmer) to make a a 1:1 dilution into each well ...
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bioRxiv - Cancer Biology 2023Quote: ... LN-229 cells were seeded at a density of 70,000 cells/well on poly-D-Lysine-coated ViewPlateTM-96 F TC 96-well black polystyrene clear bottom microplates (PerkinElmer, #6005182) in 100µl full medium ...
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bioRxiv - Cancer Biology 2023Quote: A total of 1 × 106 Bmal1::luciferase MAFs or Per2::luciferase PDA cells were seeded per well into 24-well black-sided imaging plates (1450-606, Perkin Elmer). Cells were treated with DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The luciferase reporter expression in cancer cells was examined based on bioluminescence using the IVIS imaging system (PerkinElmer, Hopkin, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... and five fields of view per well were imaged under a 4x objective in the bright and DAPI channel of a MuviCyte Live-Cell Imaging system (#HH40000000, Perkin Elmer). Cell numbers were determined by ImageJ analysis (V1.53t ...
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bioRxiv - Immunology 2024Quote: ... was added and live target cells were quantified according to the manufacturer’s protocol with a Victor Nivo® multimode plate reader (Perkin Elmer). An equal number of the target cells alone was used to denote ‘hundred percent luminescence activity’.
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bioRxiv - Neuroscience 2024Quote: The analysis of the nuclear translocation of endogenous TFEB has been performed as follow: 10k cells per well were plated in 96 well plates (PerkinElmer, 6055302) and transfected in suspension using Lipofectamine LTX (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Hep G2 and U-2 OS cells are counted and seeded into 384 well plates (PhenoPlate 384-well, Perkin Elmer, 6057328) using a Biotek or Multidrop microplate dispenser ...
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bioRxiv - Synthetic Biology 2024Quote: ... subtilis LLO after being added to BMDMs in 96-well black glass-bottom plate (50,000 cells/well; Perkin Elmer, cat# 6005430) as described above ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Binding reactions were incubated for 2 hours at room temperature and then terminated by filtration over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by three consecutive wash steps using ice-cold buffer [50 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... was performed by adding an equal volume of Nano-Glo reagent into cells suspension on black 96-well plate (Perkin Elmer). After 3 minutes of incubation at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: 1,500-2,000 SH-EP cells expressing the doxycycline-inducible shRNA of interest were seeded per well in a 384 well format (PerkinElmer/Revvity). After doxycycline (1 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... to obtain the desired number of cells for the screen and seeded in 384-well plates (ViewPlate-384 Black, Perkin Elmer) using MultiDrop combi (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: Posttranslational labeling was performed by incubating cells in normal growth medium containing 400 mCi/mL [3H]glutamine (PerkinElmer, 30-60 Ci/mmol) in the presence of (100 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Cell Biology 2020Quote: ... was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer, USA). Measurements were done at least in triplicates and relative luciferase units (RLU ...
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bioRxiv - Biochemistry 2022Quote: ... 48 hours post infection 50 µL of medium was removed from all cells were lysed by the addition of 50 µL BriteLite™ assay readout solution (Perkin Elmer) into each well ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of the virus or virus-antibody complex was added to each well in triplicates and allowed to incubate for 6 hrs before the cells were lysed with 50 µl/well of steadylite plus luciferase reagent (Perkin Elmer; 6066751). The luciferase activity was quantified using a BMG PHERAstar FS plate reader.
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bioRxiv - Immunology 2022Quote: ... Michael Farzan) or 293 flpin-TMPRSS2-ACE2 cells (made at the VRC) were plated into 96-well white/black Isoplates (PerkinElmer, Waltham, MA) at 75,00 cells per well the day before infection of pseudovirus ...
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bioRxiv - Systems Biology 2021Quote: ... The His6-MBP-PDZ concentrations of soluble cell lysate fractions were evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and were adjusted to 4 μM by dilutions ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of luciferase production from glioblastoma cells were measured using D-luciferin (150 μg/ml final concentration, Perkin Elmer, Waltham, MA, USA) and luminescence measurement in a plate reader after 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The screening was performed at same early cell passages for both replicate experiments according to the optimized amount of cells seeded in 384-well plates (ViewPlate-384 Black Perkin Elmer, #6007460) using a Multidrop Combi (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Cells were imaged on a Nikon Ti-E microscope with a CSU-X1 Yokogawa spinning-disk confocal scan head (PerkinElmer, Wellesley, MA), an Andor iXon+electron-multiplying charge-coupled device camera (Andor) ...
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bioRxiv - Cancer Biology 2020Quote: Resulting cells were ultra-concentrated in 10 μl of DPBS and plated onto wells of a CellCarrier-96 Ultra Microplates (PerkinElmer, Cat# 6055302). Plates were then centrifuged at 1800g for 10 minutes to allow the cells stick to the surface of the wells ...
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bioRxiv - Cancer Biology 2021Quote: ... the Golgi region of the cells expressing the GT-pHluorin were imaged by using the Operetta™ high content imaging system (PerkinElmer Inc.). In situ pH calibration was done by replacing the bath solution with different pH calibration buffers (125 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Microbiology 2021Quote: The cytotoxic effect of the wild-type and mutant strains were analyzed by live/dead cell assay using the High Content Screening (HCS) System (Operetta, Perkin Elmer, USA). At 12 h of post-infection ...
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bioRxiv - Synthetic Biology 2022Quote: ... Medium was removed from all wells and cells were lysed by the addition of 100 μL BriteLite™ assay readout solution (Perkin Elmer) into each well ...
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bioRxiv - Genetics 2020Quote: ... An amount of 10,000 cells/well resuspended in 60 μl of culture media was used in order to perform AlphaLISA® assay (PerkinElmer, MA, US) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Embryoid body differentiation was initiated on day 0 by detaching 70-80% confluent iPSCs with 0.5 mM EDTA and seeding 9 000 cells/well on ultra-low attachment U-bottom 96-well plates (CellCarrier-96 Spheroid ULA, Perkin Elmer 6055330) in 150μl of E8+0.5% P/S supplemented with 20 μM ROCK inhibitor ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Luciferase activity was measured for 2 seconds in intact living cells using a microBeta Wallac luminometer (Perkin Elmer, Villebon sur Yvette, France). Tests were performed in quadruplicate in at least three independent experiments and data were expressed as mean ± s.e.m ...
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bioRxiv - Biochemistry 2024Quote: ... images were captured from 15 or 5 fields per well and the number of punctate per cell were quantitated using high-content imaging and analysis software (Perkin Elmer; Harmony).
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bioRxiv - Cell Biology 2024Quote: ... transfected cells were harvested using phenol red-free DMEM and seeded at a density of 1.0 × 104 cells/well onto CellCarrierTM-96 Ultra microplates (Perkin Elmer, Waltham, MA). Then ...
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bioRxiv - Biochemistry 2024Quote: ... whereafter lysed cells were added to 2 mL scintillation fluid and radioactivity was measured using a scintillation counter (Perkin Elmer 2450 Microbeta2). Data were analyzed using GraphPad Prism 8.0.0 and fitted to a four-parameter logistic curve from where the IC50 and the 95% confidence interval were calculated ...