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Citations for PerkinElmer :
1601 - 1650 of 1735 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: TZM-bl target cells (NIH AIDS reagent program) were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of cells from each culture pulsed with 1 μCi [γ32P]-ATP having a specific activity of 30 Ci/mmol (Perkin Elmer) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 20 mM HEPES (pH 7.2 were seeded 48 h before treatment at a density of 15,000 cells per well in 96-well imaging plates (PerkinElmer cat. 6005182). Inhibitors ...
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bioRxiv - Microbiology 2021Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infectivity was considered positive when the RLU value was at least 5-fold higher than the background RLU value in the parental NP-2 cell line ...
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bioRxiv - Microbiology 2021Quote: ... a 150-μl volume of cells was resuspended and mixed by pipette with 50 μl of the luciferase substrate reagent BriteLite Plus (Perkin Elmer) in white 96-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... 30 μL of the cell suspension was plated in duplicate into wells of a 384-well white-walled plate (PerkinElmer CulturePlate) and coelenterazine-h was added to a final concentration of 3.375 μM ...
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bioRxiv - Cell Biology 2021Quote: ... 90 μL of the cell suspension was also plated in duplicate into wells of a 96-well black-walled plate (PerkinElmer OptiPlate), and Venus fluorescence was determined using an excitation wavelength of 485 nm (5 nm bandwidth ...
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bioRxiv - Cancer Biology 2020Quote: 2D adherent studies – Cells were seeded at 500-1,000 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 48 hours prior to drug treatment ...
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bioRxiv - Neuroscience 2020Quote: ... were detached by pipetting and 104 cells in 40 µl of medium were seeded per well of a 384-well plated (Perkin Elmer) and cultured for one additional day ...
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bioRxiv - Microbiology 2020Quote: ... log phase promastigotes were washed twice with PBS and resuspended at 5.0 x 107 cells/ml in PBS in the presence of 100 µM of D-[U-14C] glucose (250 mCi/mmol, PerkinElmer Inc.). The reaction mixture (200 µl each in triplicates ...
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bioRxiv - Systems Biology 2021Quote: ... cell cultures were seeded and infected at the desired MOI in black 96-well PerkinElmer CellCarrier ultra imaging plates (Perkin Elmer). Infected cells were fixed with 10% neutral buffered formalin (NBF ...
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bioRxiv - Microbiology 2021Quote: ... HEV replication was analyzed by determining the number of GFP-positive cells using fully automated confocal microscopy (Operetta CLS; PerkinElmer Devices).
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bioRxiv - Microbiology 2020Quote: ... CoV-infected cells and control mock-infected cells were incubated for different periods of time with tritiated uridine ([5-3H]uridine, 1 mCi/ml, Perkin Elmer), which was mixed in a 1:1 ratio with double-concentrated medium ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Cell Biology 2021Quote: Parental and SETD2-deleted U2OS cells were seeded into 384-well plates (750 cells in 75 μl per well) using the Janus Liquid Handling Workstation (Perkin Elmer) and incubated at 37°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Radioactivity of both the supernatant and the cells was measured by the 2480 Wizard2 Automatic Gamma Counter (PerkinElmer, Waltham, MA, USA). Uptake values were adjusted for tracer decay and corrected for cell amount.
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bioRxiv - Cell Biology 2021Quote: ... and dead cells (stained by PI+) were imaged every hour for the indicated time with the Operetta CLS High-Content microscope (Perkin Elmer) at 40x Air/0.6 NA ...
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bioRxiv - Cell Biology 2021Quote: ... samples of doublets were allocated by semi-automated 3D intensity quantification in the Wnt3a-bead proximal and -distal cell using Volocity (Perkin Elmer) or Fiji (ImageJ) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were imaged and percent of virus infected cells calculated using the Operetta High Content Imaging System and Harmony® High Content Imaging and Analysis Software (PerkinElmer).
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Cancer Biology 2022Quote: MDA-MB-231 cells expressing mTurquoise2-SLBP(18-126) and H1-Maroon1 were seeded on clear flat-bottom 96-well plates (Perkin Elmer), treated with DMSO or PXS5382 40μM ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
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bioRxiv - Neuroscience 2021Quote: ... Absorbance of 100 µl aliquots of solubilized cells was measured at 595 nm with Wallac Victor 1420 microplate reader (Perkin Elmer).
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bioRxiv - Neuroscience 2021Quote: ... the fluorescently labeled axon and dendrites were traced from the PV basket cell soma with a Nipkow spinning disk confocal microscope (Volocity, Perkin Elmer, equipped with an Orca camera ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plate was sealed with a transparent film and cells were imaged with the Operetta High Content Imager (PerkinElmer Life Sciences) using a 20x objective ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysed cells were transferred to vials and measured in a gamma counter (Wallac 1470 Wizard, Perkin Elmer, Waltham, MA, USA). Obtained cpm values were plotted with GraphPad Prism and the data fitted using nonlinear regression (one site - fit Ki ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed with KREBS solution and fluorescence was read with the plate reader VICTOR® Multilabel Plate Reader (PerkinElmer). Data were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... For neutralization assays 104 293T-hACE2 cells were plated in 100uL media per well in 96 well white-wall white-bottom plates (Perkin Elmer) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
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bioRxiv - Biophysics 2020Quote: ... The undifferentiated cells were seeded to 60% confluency to adhere for 24 h in a CellCarrier-96 Ultra Microplates (Perkin Elmer), which was followed by the media replacement with a fresh media containing FCS reduced to 1% and supplemented with 10 μM trans-Retinoic acid (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
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bioRxiv - Cell Biology 2021Quote: The data was collected in a set of 2 experiments for 9 compounds as follows: 384 well plates (PE cell carrier ultra, PerkinElmer, 6057308) containing 12,000 cells per well in RPMI1640 medium (Sigma ...
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bioRxiv - Immunology 2022Quote: ... incubated for 45 minutes at 37 °C and added to pre-seeded 293 flpin-TMPRSS2-ACE2 cells (made by Adrian Creanga, VRC, NIH) in triplicate in 96-well white/black Isoplates (Perkin Elmer). Following 2 hours of incubation ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells were incubated with the AAVs for 24 hours at 37°C and assayed with Britelite plus (Perkin Elmer, cat#6066766) following the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were shaken for 24 hours at 250 RPM and at 37°C whereupon the Breathe Easy sealing membranes were removed and cell density was measured using OD600 in a Victor NIVO microplate reader (Perkin Elmer). OD600 of cell-free medium was removed from all cell density values ...
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bioRxiv - Cell Biology 2024Quote: ... Cholesterol efflux was determined as % counts per minute (CPM) in the supernatant versus cell lysates with a liquid scintillation analyzer (Tricarb-2810TR, Perkin Elmer).
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bioRxiv - Neuroscience 2024Quote: ... We fixed and stained cells on Prime automated liquid handling system (HighRes Biosolutions, Beverly, MA) and imaged on the Opera Phenix (Perkin Elmer) using a 20x water objective (Perkin Elmer) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were counterstained with DAPI (1 μg ml-1) before imaging with the Opera Phenix High Content Screening System (Perkin Elmer).
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bioRxiv - Cell Biology 2023Quote: ... and cells were seeded in 96-well plates under normal growth conditions and fluorescence from tracked live cells was measured using the Operetta High Content Screening System (Perkin Elmer).
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bioRxiv - Cancer Biology 2024Quote: ... The total distance of invasion from the center of the spheroid was calculated by summing up the individual invasion distances of each cell using Harmony (Perkin Elmer). The data was displayed and statistically analyzed using Prism 9.3.1 software (GraphPad).
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bioRxiv - Biophysics 2024Quote: Single-point FCS data were acquired with an Insight Cell confocal microscope (Evotec Technologies, Hamburg, Germany, now PerkinElmer, Waltham, MA, USA). Excitation was performed using a 488 nm continuous wave solid state diode-pumped laser (Sapphire 488-20/460-10 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then maintained at constant 37°C for 36 hours before moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Immunology 2022Quote: ... the medium was removed from all wells and cells were lysed by the addition of 100 µl of a 1:1 dilution of BriteLite assay readout solution (Perkin Elmer) and 1xDPBS (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... 30,000 cells were seeded in each well of an ultra-low adhesive U-shaped 96-well plate (CellCarrier™ −96, PerkinElmer) with 200 ml of complete Neurobasal medium ...
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bioRxiv - Genomics 2023Quote: ... Plates were shaken and incubated for 3h at cell culture conditions before measuring the fluorescence (560Ex/590Em) on an EnVision Multilabel Plate Reader (Perkin Elmer). The lowest fluorescence from the PAO killing control in each replicate was subtracted from all the samples within the sample replicate to standardize the raw values ...