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Citations for PerkinElmer :
1351 - 1400 of 1735 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Immunology 2020Quote: ... The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer, Waltham, MA, USA). The libraries were qPCR quantified (Kapa Biosystems ...
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bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays, Perkin Elmer, Codolet, France) according to the manufacturer’s recommendations (112) ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular ATP and extracellular lactate levels were measured for each NCC primary cultures after Seahorse analysis using an ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943) and the Lactate Fluorometric Assay kit from BioVision (cat ...
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bioRxiv - Physiology 2020Quote: ... 1 ng of eluted and purified DNA was used to prepare DNA sequencing library with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, NOVA-5188-01) on the NextSeq 500 system (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Genetics 2020Quote: ... and used as input for small RNA library prep using the NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific - PerkinElmer NOVA-5132-06). Libraries were prepared according to manufacturer’s instructions (V19.01) ...
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bioRxiv - Plant Biology 2020Quote: The AlphaScreen® (Amplified Luminescent Proximity Homogeneous Assay) was carried out using an AlphaScreen® FLAG® (M2) Detection Kit (Perkin Elmer, MA) to detect protein-protein interactions ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Microbiology 2021Quote: DNA library preparation was carried out according to the manufacturer’ ss protocol using the NEXTflex™ ChIP-Seq Kit (NOVA-5143-01) (PerkinElmer, Hamburg, Germany) and the NEXTflex™ ChIP-Seq Barcodes (NOVA-514122 ...
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bioRxiv - Genomics 2022Quote: ... 1 ng of eluted and purified DNA was used to prepare libraries with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, #NOVA-5188-01) on the Illumina NextSeq500 system using a single read 100 bp protocol ...
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bioRxiv - Microbiology 2022Quote: Quality and quantity of the extracted RNA samples were analyzed with LabChip GX Touch HT RNA Assay Reagent Kit (PerkinElmer, Waltham, MA, USA) and Qubit RNA BR kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA libraries were prepared from RNA extracts with a minimum RNA integrity number (RIN) of 9.5 using the NEXTFLEX® Combo-Seq mRNA/miRNA Kit (PerkinElmer®, Waltham, MA). Libraries were quantified using a KAPA Library Quantification Kit (Roche Sequencing and Life Science ...
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bioRxiv - Biochemistry 2023Quote: The expression of IL-6 and TNF-α in culture supernatants was detected using the corresponding AlphaLISA Immunoassay Kit (PerkinElmer, AL223C and AL208C) according to the manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2022Quote: ... Extracted DNA was sent to Texas A&M Agrilife Bioinformatics and Genomics Service (College Station, TX, USA) for whole-genome library preparation using a NEXTFLEX Rapid XP DNA-Seq Kit HT (PerkinElmer, Waltham, MA, USA). Libraries were sequenced at the Texas A&M Agrilife Bioinformatics and Genomics Service on one NovaSeq 6000 S4 lane at 26.1 ± 5.8 depth of coverage per sample across the full genome (2 s.d. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The staining and fixation were performed following the published protocol (Cimini et al., 2023) with the use of PhenoVue JUMP kit (Perkin Elmer, ref.: PING23). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: Intracellular ATP and glutamine/glutamate levels were measured for each NCC primary cultures after Seahorse analysis using the ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943), and the Glutamine/Glutamate-Go assay (J8021 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... These VHHs were screened for direct binding to the biotinylated hMOG using the AlphaScreen FLAG (M2) Detection Kit (6760613, PerkinElmer, Waltham, MA, USA) and to the mMOG overexpressing CHO cells using flow cytometry with mouse-anti-FLAG-iFluor647 Ab (Genscript ...
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bioRxiv - Cancer Biology 2021Quote: ... Number of basal and suprabasal cells in each clone was counted using Volocity 6 Image Analysis software (PerkinElmer).
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bioRxiv - Cell Biology 2022Quote: ... Images of spheroids and invaded cells were acquired on an Operetta CLS High-Content Analysis System (PerkinElmer, HH16000000) at 5x magnification using the Dapi (405 nm ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with mESC medium supplemented with 0.8 Mbq/mL of 35S Protein Labeling Mix (Perkin Elmer) at 37 °C with 5 % CO2 for 30 min ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed and Luc activity determined using a microtiter plate luminometer and BriteLite Plus Reagent (Perkin Elmer). Neutralization titers were the sample dilution at which relative luminescence units (RLU ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were imaged at 37°C and 5% CO2 using Operetta CLS high-content analysis system (PerkinElmer) using DAPI and TRITC filters ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were imaged at 37°C and 5% CO2 using Operetta CLS high-content analysis system (PerkinElmer) using DAPI and TRITC filters ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells in 96-well plates were imaged using an Opera Phenix high-content screening system (PerkinElmer, USA) equipped with a 20x/0.4 NA objective ...
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bioRxiv - Cancer Biology 2021Quote: ... For single cell invasion analysis spheroids in the above conditions were imaged using an Opera Phenix (Perkin Elmer) with a 5x (need NA ...
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bioRxiv - Cell Biology 2021Quote: Live cell labeling of hydroxyapatite (HA) was performed with 1 μM OsteoSense 680EX Fluorescent Imaging Agent (Perkin Elmer) in BRDM for 20 minutes at 37 °C.
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bioRxiv - Cancer Biology 2020Quote: ... the luminescence intensity of the transfected cells was measured using a 2030 ARVO X multi-label counter (PerkinElmer) or a GloMax® Explorer Multimode Microplate Reader 3500 (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: Single-cell fluorescence of the samples was then analyzed by flow cytometry using a GALLIOS cytometer (Perkin Elmer) or with a MACSQuant™ VYB cytometer (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were imaged using an automated Opera Quadrupule Enhanced High Sensitivity (QEHS) spinning-disk confocal microscope (Perkin Elmer) with 20x air lens ...
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bioRxiv - Cell Biology 2022Quote: ... 2000 cells at passage 5 were plated in a poly-D-lysine coated 384-CellCarrier microtiter plate (PerkinElmer) in FBM with FGM-2TM Single Quots (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... THP-1 cells were plated in a 24-well optical bottom tissue culture plate (Perkin Elmer, 1450-606) by aliquoting 500 μL of this cell suspension to each well and then incubating at 37°C for two days ...
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bioRxiv - Cancer Biology 2022Quote: ... Bioluminescence (radiance/sec) emitted from the cells were quantified using the IVIS Spectrum and Living Image software (PerkinElmer) of the animals ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µL containing 2,500 cells/well were seeded on 384 well-plates (Perkin Elmer, PhenoPlate 384-well microplates) containing pre-dispensed compounds dissolved in DMSO.
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bioRxiv - Cell Biology 2022Quote: Cells in passage 5 were plated in a poly-D-lysine coated 384 CellCarrier microtiter plate from PerkinElmer in fibroblast basal medium (FBM ...
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bioRxiv - Immunology 2021Quote: ... The remaining cell-bound radioactivity was measured using a Wizard² 2480 gamma counter (PerkinElmer Inc., Waltham, MA, USA) and quantified as percentage of total added activity.
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bioRxiv - Microbiology 2020Quote: ... Protein localization in fungal cells was carried out by an UltraVIEW VoX Spinning Disk Confocal Microscope (PerkinElmer, USA) and a Leica DMi8 (Leica ...
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bioRxiv - Cancer Biology 2022Quote: High-Throughput immunofluorescence analysis was performed with cells seeded on clear flat-bottom 96-well plates (Perkin Elmer), treated as described in the manuscript ...
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bioRxiv - Pathology 2021Quote: – For palmitate oxidation assay: Cells were preincubated for 3 hours with 14Cpalmitate (1uCi/mL; Perkin Elmer, Boston MA) and non labeled (cold ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293T cells grown in 96-well plates were imaged using an Opera Phenix high-content screening system (PerkinElmer) equipped with a 20x/0.4 NA objective ...
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bioRxiv - Neuroscience 2021Quote: ... maintaining cells at culture conditions (37 ºC and 5% CO2) using high-content imaging system Operetta® (PerkinElmer) and analyzed using Harmony® software.
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bioRxiv - Cancer Biology 2021Quote: ... cells (106) were washed and resuspended in 100 μl PBS placed in OptiPlate-96 (PerkinElmer, Waltham, MA, USA) in the presence or absence of indicated peptides (50 μM ...
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was quantified by measuring luciferase activity in cell lysates using Britelite Plus (Perkin Elmer, Richmond, California) and following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... The cells were imaged at 37 °C and 5% CO2 on Operetta CLS high-content imaging system (PerkinElmer) by using DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... After incubation cells were exposed to 2X Hot Lactate/Pyruvate solution (0.5µCi/mL of 14C-L-lactate/14C Pyruvate, PerkinElmer) for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... RAB12 KO and LRRK2 KO A549 cells were seeded in 96 well plates (PerkinElmer, Waltham, MA, Phenoplate, #6055302), and then treated with vehicle or LLOMe (1 mM) ...
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bioRxiv - Bioengineering 2023Quote: ... The IVISbrite 4T1 Red F-luc Bioluminescent Tumour Cell line (Bioware Brite) was purchased from PerkinElmer (Waltham, MA). Fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in 3x in PBS and imaged on the Opera Phenix High-Content Screening System (PerkinElmer) using the Harmony analysis software.
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bioRxiv - Cancer Biology 2023Quote: ... target cells were loaded with an acetoxymethyl ester of the fluorescence-enhancing ligand (BATDA; Perkin Elmer #C136-100) and then co-cultured at 10,000 cells/well in triplicate with effector cells at the indicated E:T ratios ...