Labshake search
Citations for PerkinElmer :
1601 - 1650 of 1684 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... We verified that these cells did not lose viability after overnight culture in serum-free medium relative to medium with 10% serum as determined by cell-based measurements of CBG bioluminescence in an IVIS Lumina (Perkin Elmer). We injected 1 × 105 breast cancer cells per mouse (n = 5 per each cell type) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and then acquired a bioluminescence image of total viable cells on an IVIS Lumina (30 second image, large field of view) (Perkin Elmer). A person blinded to experimental conditions enumerated colonies and quantified imaging data (Living Image ...
-
bioRxiv - Genomics 2023Quote: ... Plates were shaken and incubated for 3h at cell culture conditions before measuring the fluorescence (560Ex/590Em) on an EnVision Multilabel Plate Reader (Perkin Elmer). The lowest fluorescence from the PAO killing control in each replicate was subtracted from all the samples within the sample replicate to standardize the raw values ...
-
bioRxiv - Cell Biology 2023Quote: MIN6 cells were seeded at a density of 15,000 per well in a 384-well plate (Perkin Elmer, CellCarrier Ultra, #6057300). After 24 h recovery ...
-
bioRxiv - Immunology 2023Quote: ... cells were isolated ex-vivo and then incubated for six hours with 0.5 μCi 14C-palmitic acid (Perkin Elmer Cat# NEC075H050UC) in the following media ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 25μL was added to the transfected cells, after which the baseline luminescence was measured for 15min (continuous mode, 1min intervals) using an Envision (Perkin Elmer) spectrophotometer ...
-
bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
-
bioRxiv - Cell Biology 2024Quote: ... Cholesterol efflux was determined as % counts per minute (CPM) in the supernatant versus cell lysates with a liquid scintillation analyzer (Tricarb-2810TR, Perkin Elmer).
-
bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... The luciferase reporter expression in cancer cells was examined based on bioluminescence using the IVIS imaging system (PerkinElmer, Hopkin, MA, USA).
-
bioRxiv - Cancer Biology 2023Quote: ... and five fields of view per well were imaged under a 4x objective in the bright and DAPI channel of a MuviCyte Live-Cell Imaging system (#HH40000000, Perkin Elmer). Cell numbers were determined by ImageJ analysis (V1.53t ...
-
bioRxiv - Cancer Biology 2023Quote: A total of 1 × 106 Bmal1::luciferase MAFs or Per2::luciferase PDA cells were seeded per well into 24-well black-sided imaging plates (1450-606, Perkin Elmer). Cells were treated with DMSO ...
-
bioRxiv - Cancer Biology 2023Quote: ... LN-229 cells were seeded at a density of 70,000 cells/well on poly-D-Lysine-coated ViewPlateTM-96 F TC 96-well black polystyrene clear bottom microplates (PerkinElmer, #6005182) in 100µl full medium ...
-
bioRxiv - Cell Biology 2023Quote: ... and cells were seeded in 96-well plates under normal growth conditions and fluorescence from tracked live cells was measured using the Operetta High Content Screening System (Perkin Elmer).
-
bioRxiv - Microbiology 2024Quote: ... Cultures were shaken for 24 hours at 250 RPM and at 37°C whereupon the Breathe Easy sealing membranes were removed and cell density was measured using OD600 in a Victor NIVO microplate reader (Perkin Elmer). OD600 of cell-free medium was removed from all cell density values ...
-
bioRxiv - Neuroscience 2024Quote: ... We fixed and stained cells on Prime automated liquid handling system (HighRes Biosolutions, Beverly, MA) and imaged on the Opera Phenix (Perkin Elmer) using a 20x water objective (Perkin Elmer) ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were counterstained with DAPI (1 μg ml-1) before imaging with the Opera Phenix High Content Screening System (Perkin Elmer).
-
bioRxiv - Biochemistry 2024Quote: ... then re-formatted to acoustic dispensing-enabled screening 1536-well plates using automated robotic systems cell∷explorer (PerkinElmer, Waltham, MA, USA) and Vantage (Hamilton ...
-
bioRxiv - Biophysics 2024Quote: Single-point FCS data were acquired with an Insight Cell confocal microscope (Evotec Technologies, Hamburg, Germany, now PerkinElmer, Waltham, MA, USA). Excitation was performed using a 488 nm continuous wave solid state diode-pumped laser (Sapphire 488-20/460-10 ...
-
bioRxiv - Cancer Biology 2024Quote: ... The total distance of invasion from the center of the spheroid was calculated by summing up the individual invasion distances of each cell using Harmony (Perkin Elmer). The data was displayed and statistically analyzed using Prism 9.3.1 software (GraphPad).
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then maintained at constant 37°C for 36 hours before moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
-
bioRxiv - Microbiology 2020Quote: ... The DNA concentration was measured using the Picogreen method (Invitrogen Quant-iT™ Picogreen dsDNA Assay Kit Reference No. P11496 on Perkin Elmer 2030 Multilabel Reader Victor X3) and DNA integrity number (DIN ...
-
bioRxiv - Cell Biology 2020Quote: Posttranslational labeling was performed by incubating cells in normal growth medium containing 400 mCi/mL [3H]glutamine (PerkinElmer, 30-60 Ci/mmol) in the presence of (100 µg/ml ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
-
bioRxiv - Cell Biology 2020Quote: ... was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer, USA). Measurements were done at least in triplicates and relative luciferase units (RLU ...
-
bioRxiv - Biochemistry 2022Quote: ... 48 hours post infection 50 µL of medium was removed from all cells were lysed by the addition of 50 µL BriteLite™ assay readout solution (Perkin Elmer) into each well ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 µl of the virus or virus-antibody complex was added to each well in triplicates and allowed to incubate for 6 hrs before the cells were lysed with 50 µl/well of steadylite plus luciferase reagent (Perkin Elmer; 6066751). The luciferase activity was quantified using a BMG PHERAstar FS plate reader.
-
bioRxiv - Immunology 2022Quote: ... Michael Farzan) or 293 flpin-TMPRSS2-ACE2 cells (made at the VRC) were plated into 96-well white/black Isoplates (PerkinElmer, Waltham, MA) at 75,00 cells per well the day before infection of pseudovirus ...
-
bioRxiv - Cell Biology 2019Quote: ... The library was diluted to a 5 μM stock with 1x siRNA buffer (GE Dharmacon, B-002000-UB-100) and arrayed onto black 384-well PerkinElmer Cell Carrier plates (Perkin Elmer, 6007550) using the Echo Liquid Handler (Labcyte) ...
-
bioRxiv - Systems Biology 2021Quote: ... The His6-MBP-PDZ concentrations of soluble cell lysate fractions were evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and were adjusted to 4 μM by dilutions ...
-
bioRxiv - Bioengineering 2020Quote: A spheroid of 4434 BRAF mutant mouse melanoma cells embedded in Matrigel in one well of a plastic bottomed 96-well plate (PerkinElmer, CellCarrier, #6005550) was imaged with the dOPM system ...
-
bioRxiv - Bioengineering 2020Quote: ... Levels of luciferase production from glioblastoma cells were measured using D-luciferin (150 μg/ml final concentration, Perkin Elmer, Waltham, MA, USA) and luminescence measurement in a plate reader after 15 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... The screening was performed at same early cell passages for both replicate experiments according to the optimized amount of cells seeded in 384-well plates (ViewPlate-384 Black Perkin Elmer, #6007460) using a Multidrop Combi (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: Cells were imaged on a Nikon Ti-E microscope with a CSU-X1 Yokogawa spinning-disk confocal scan head (PerkinElmer, Wellesley, MA), an Andor iXon+electron-multiplying charge-coupled device camera (Andor) ...
-
bioRxiv - Cancer Biology 2020Quote: Resulting cells were ultra-concentrated in 10 μl of DPBS and plated onto wells of a CellCarrier-96 Ultra Microplates (PerkinElmer, Cat# 6055302). Plates were then centrifuged at 1800g for 10 minutes to allow the cells stick to the surface of the wells ...
-
bioRxiv - Cancer Biology 2021Quote: ... the Golgi region of the cells expressing the GT-pHluorin were imaged by using the Operetta™ high content imaging system (PerkinElmer Inc.). In situ pH calibration was done by replacing the bath solution with different pH calibration buffers (125 mM KCl ...
-
bioRxiv - Microbiology 2019Quote: ... Parasite numbers were correlated with bioluminescence intensity by plating a two-fold serial dilution of cells.10 Plates were read with an EnVision Multilabel Plate Reader (Perkin Elmer, USA) after incubation at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Microbiology 2021Quote: The cytotoxic effect of the wild-type and mutant strains were analyzed by live/dead cell assay using the High Content Screening (HCS) System (Operetta, Perkin Elmer, USA). At 12 h of post-infection ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Medium was removed from all wells and cells were lysed by the addition of 100 μL BriteLite™ assay readout solution (Perkin Elmer) into each well ...
-
bioRxiv - Genetics 2020Quote: ... An amount of 10,000 cells/well resuspended in 60 μl of culture media was used in order to perform AlphaLISA® assay (PerkinElmer, MA, US) according to manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: Flp-In-CHO cells either stably or transiently expressing the M2/M5 mAChR chimeras were seeded in 96-well Isoplates (PerkinElmer Life Sciences) at a concentration of 20,000 – 25,000 cells per well a day before the experiment was performed in a humidified atmosphere at 37°C ...
-
bioRxiv - Neuroscience 2019Quote: ... sections were then processed for visualization using the RNA-scope Multiplex Fluorescent reagent Kit v2 (Advanced Cell Diagnostics) and the Tyramide Signal Amplification (TSA™) Plus Cyanine 3 and TSA Plus Cyanine 5 systems (Perkin Elmer).
-
bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
-
bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
-
bioRxiv - Neuroscience 2020Quote: ... Embryoid body differentiation was initiated on day 0 by detaching 70-80% confluent iPSCs with 0.5 mM EDTA and seeding 9 000 cells/well on ultra-low attachment U-bottom 96-well plates (CellCarrier-96 Spheroid ULA, Perkin Elmer 6055330) in 150μl of E8+0.5% P/S supplemented with 20 μM ROCK inhibitor ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Luciferase activity was measured for 2 seconds in intact living cells using a microBeta Wallac luminometer (Perkin Elmer, Villebon sur Yvette, France). Tests were performed in quadruplicate in at least three independent experiments and data were expressed as mean ± s.e.m ...
-
bioRxiv - Biochemistry 2023Quote: ... The other procedures were the same as the Zn transport assay except that the cell lysates were mixed with the Ultima Gold cocktail (PerkinElmer, Cat# L8286) and 55Fe associated with the cells was quantified using a liquid scintillation counter (LS 6500 Multi-Purpose Scintillation Counter).