Labshake search
Citations for PerkinElmer :
1201 - 1250 of 1684 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Automated (unbiased) confocal single-cell fluorescence measurement was performed by Operetta CLS™ (Operetta– Perkin Elmer) at 40× magnification.
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bioRxiv - Molecular Biology 2023Quote: iBMDM (3*104 cells/well) were seeded in a 96-well plastic-bottom imaging plate (PerkinElmer) overnight and then infected with C ...
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bioRxiv - Microbiology 2023Quote: ... 16,000 effector cells were detached and reseeded into a 96-well black plate (PerkinElmer, Cat# 6005225), and target cells were reseeded at a density of 1,000,000 cells/2 ml/well in 6-well plates ...
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bioRxiv - Molecular Biology 2023Quote: iBMDM (3*104 cells/well) were seeded in a 96-well plastic-bottom imaging plate (PerkinElmer) overnight ...
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bioRxiv - Molecular Biology 2023Quote: iBMDM (3*104 cells/well) were seeded in a 96-well plastic-bottom imaging plate (PerkinElmer) overnight and then infected with C ...
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bioRxiv - Molecular Biology 2023Quote: iBMDM (3*104 cells/well) were seeded in a 96-well plastic-bottom imaging plate (PerkinElmer) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... CaAM signal from the invaded cells was measured by a plate reader (Perkin Elmer Victor X3) at 465/535nm.
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence from tracked live cells was measured using the Operetta High Content Screening System (Perkin Elmer), using 20x magnification (see ‘Microscopy’ methods for more details) ...
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bioRxiv - Cell Biology 2023Quote: Live cell time-lapse imaging was performed using the Operetta High Content Screening System (Perkin Elmer) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated according to the manufacturer’s guidelines and proceeded to EnVision Multimode Plate Reader (PerkinElmer) for data acquisition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and cells were lysed by the addition of 100 μl of BriteLite assay readout solution (PerkinElmer) into each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5×10^3 LN-229 cells/well were plated in CellCarrier-96 Ultra Microplates (PerkinElmer, #6055302) 24 hours prior to the experiment ...
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bioRxiv - Biochemistry 2024Quote: ... CHO-K1 cells were plated at 50,000/well in white/clear bottom 96 well Isoplates (PerkinElmer) in growth media a day before the experiments ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Then the yeast cells were then continuously imaged under high-content fluorescence microscopy (PerkinElmer Operetta CLS) for 10 hours with 1-hour intervals to observe the individual offspring of GFP-yeast ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µL of the cell suspension was seeded into a black 96-well plate (Perkin Elmer) and Calcium Green-5N fluorescence (excitation 506 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... Segmentation and counting of GFP- and mCherry-positive cells were performed using an Acapella script (PerkinElmer).
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bioRxiv - Cancer Biology 2024Quote: ... 1,000 to 5,000 cells were seeded into 384-well plates (CulturPlate™, Perkin Elmer, MA, USA) using a Multidrop™ Combi Reagent Dispenser (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Detection of either ERK1/2 or Akt1/2/3 phosphorylation was performed as described in the corresponding AlphaScreen SureFire kits and fluorescence measured with an EnVision plate reader (PerkinElmer, Boston, MA). Data were normalised to the response elicited upon stimulation of cells with 10% FBS.
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bioRxiv - Microbiology 2021Quote: ... 1-3ng/μl of the PCR amplifier of the HPV L1 gene was mixed with 8 μL of ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit version 1.1(Perkin Elmer Biosystems, USA), 2 pmol of primer ...
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bioRxiv - Microbiology 2021Quote: ... using 100 ng total RNA for library preparation with the NEXTFLEX® Small RNA-Seq Kit v3 (PerkinElmer Inc., Waltham, MA, USA), followed by library sequencing on one lane NovaSeq6000 SP v1.0 (2×50bp) ...
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bioRxiv - Microbiology 2019Quote: ... bacteria DNA was extracted using the chemagic STAR DNA BTS Kit for the automated isolation of DNA (Perkin Elmer, Wellesley, MA, USA). Quality and purity of the isolated DNA were checked by spectrophotometry on the NanoDrop (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Physiology 2020Quote: Renal tissue cAMP production following ligand stimulation was measured using the time-resolved fluorescence energy transfer (TR-FRET) based LANCE ULTRA cAMP Kit (PerkinElmer, MA, USA) in combination with an EnSight Multimode Plate Reader (Perkin Elmer ...
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bioRxiv - Neuroscience 2020Quote: ... 75 µL of supernatant from each well was collected for ATP supernatant detection using the ATPlite Luminescence Assay Kit (Perkin Elmer, 6016943) as described by the kit protocol ...
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bioRxiv - Microbiology 2021Quote: ... Viral supernatant was then used to transduce each cell line of interest for 48 h and luciferase activity was measured with Promega Steady-Glo® kit (E2550) using a Victor X3 Multilabel Reader (Perkin Elmer). Each experiment shown is representative of three independent experiments.
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bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from 250 mg of leaves ground to powder under liquid nitrogen using the Chemagic DNA Plant Kit (Perkin Elmer, Germany), according to the manufacturer’s instructions on a KingFisher Flex™ (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08) as per the manufacturer’s protocol and sequenced on the Nextseq 500 instrument (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Messenger RNA was isolated from 150 ng of total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer; Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Physiology 2023Quote: ... and blood was collected for the measurement of both glucose (Accu-Check) and insulin levels (Insulin AlphaLISA detection kit, Perkin Elmer, USA).
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bioRxiv - Physiology 2023Quote: ... Perfusates were continuously and automatically collected into 96-well plates for the detection of insulin (Insulin AlphaLISA Detection Kit, Perkin Elmer, USA). Insulin measurement was performed according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... These purified ribosome footprints were used as input for the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06). The resulting cDNA was amplified by 11-16 cycles of PCR with a barcode incorporated in the primer ...
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bioRxiv - Immunology 2024Quote: ... 1:1 mixed with maleate buffer (R2a from the kit)) was added and incubated at room temperature and measured kinetically on an Envision microplate reader (PerkinElmer, Waltham, MA) at 340 nm for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood samples or buffy coat extracts were extracted in Auria Biobank for all patients for DNA extraction and genomic sequencing to serve as germline reference for identifying somatic genomic aberrations using Chemagic DNA Blood Kit Special (PerkinElmer Inc., USA) and Chemagic 360 instrument (PerkinElmer Inc. ...
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bioRxiv - Immunology 2021Quote: ... transduction was assessed by counting the GFP-expressing cells using the fluorescent plate reader EnSight (Perkin Elmer). Each serum dilution was assessed in triplicate ...
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bioRxiv - Microbiology 2022Quote: ... Parasites were lysed by freeze/thaw and were collected on filters using a cell harvester (PerkinElmer LifeSciences). This was followed by addition of MicroScint O scintillation cocktail and incorporation of 3H-hypoxanthine was measured using a TopCount scintillation counter (PerkinElmer Life-sciences).
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bioRxiv - Cell Biology 2022Quote: ... The number of ciliated cells was measured using a semi-automated analysis using Harmony software (Perkin Elmer). Briefly ...
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bioRxiv - Immunology 2019Quote: ... Cells were then distributed (80 µg of proteins per well) in a 96-well microplate (Optiplate, PerkinElmer). BRET2 between RLuc8 and GFP10 was measured 1 min after addition of 5 µM coelenterazine 400a/Deep blue C (Gentaur) ...
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bioRxiv - Microbiology 2019Quote: ... Radioactivity of sorted cell batches was measured in a liquid scintillation counter (Tri-Carb 2900, Perkin Elmer). The abundance of PSB and GSB in 14C-carbon assimilation experiments is given as relative percentage of gated cells identified as PSB/GSB out of all SYBR green-stained cells concurrently counted using flow cytometry.
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bioRxiv - Microbiology 2021Quote: ... and the percentage of GFP positive cells was enumerated (Harmony® high-content analysis software, Perkin Elmer).
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bioRxiv - Neuroscience 2020Quote: Quantification of cell types was performed using the Harmony High Content Imaging and Analysis Software (Perkin Elmer). Percentage of cells positive for desired marker versus total number of live cells was calculated ...
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bioRxiv - Biochemistry 2021Quote: ... Fully-haploid engineered HAP1 (eHAP) human cells (Essletzbichler et al., 2014) were obtained from Horizon (now PerkinElmer), and were cultured in complete IMDM medium (ThermoFisher # 12440-053 ...
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bioRxiv - Microbiology 2020Quote: ... Every 24 hours total well fluorescence and cell growth were measured using Wallac 1420 Plate Reader (PerkinElmer Life and Analytical Science ...
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bioRxiv - Microbiology 2021Quote: ... high content imaging instrument and the number of infected cells were determined using Harmony Software (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were imaged using an Opera Phenix high content screening system and analyzed using Columbus software (PerkinElmer) using 20x water objectives ...
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bioRxiv - Immunology 2021Quote: ... Cells were subsequently lysed and luciferase activity was determined using a luminometer and BriteLite Plus reagent (PerkinElmer). Neutralization titers were defined as the serum dilution which reduced relative light units by 50% relative to control wells after background subtraction.