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Citations for PerkinElmer :
1551 - 1600 of 1684 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Cells were fixed in growth medium with 3.7% formaldehyde at room temperature for 30 minutes and their cell walls digested with a solution containing 50 μg/ml zymolyase and 1:50 glusulase (Perkin Elmer). The digested cells were mounted on poly-L lysine coated slides to immobilize them for microscopy ...
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bioRxiv - Immunology 2021Quote: ... was conjugated to Alexa Fluor 594 and cells were quantified with an Operetta high content imaging system (Perkin Elmer, Waltham, MA). A minimum of 16,000 cells are counted per sample dilution across four wells - two each in duplicate plates ...
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bioRxiv - Immunology 2021Quote: TZM-bl target cells (NIH AIDS reagent program) were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of cells from each culture pulsed with 1 μCi [γ32P]-ATP having a specific activity of 30 Ci/mmol (Perkin Elmer) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 20 mM HEPES (pH 7.2 were seeded 48 h before treatment at a density of 15,000 cells per well in 96-well imaging plates (PerkinElmer cat. 6005182). Inhibitors ...
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bioRxiv - Microbiology 2021Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infectivity was considered positive when the RLU value was at least 5-fold higher than the background RLU value in the parental NP-2 cell line ...
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bioRxiv - Microbiology 2021Quote: ... a 150-μl volume of cells was resuspended and mixed by pipette with 50 μl of the luciferase substrate reagent BriteLite Plus (Perkin Elmer) in white 96-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... 30 μL of the cell suspension was plated in duplicate into wells of a 384-well white-walled plate (PerkinElmer CulturePlate) and coelenterazine-h was added to a final concentration of 3.375 μM ...
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bioRxiv - Cell Biology 2021Quote: ... 90 μL of the cell suspension was also plated in duplicate into wells of a 96-well black-walled plate (PerkinElmer OptiPlate), and Venus fluorescence was determined using an excitation wavelength of 485 nm (5 nm bandwidth ...
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bioRxiv - Cancer Biology 2020Quote: 2D adherent studies – Cells were seeded at 500-1,000 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 48 hours prior to drug treatment ...
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bioRxiv - Neuroscience 2020Quote: ... were detached by pipetting and 104 cells in 40 µl of medium were seeded per well of a 384-well plated (Perkin Elmer) and cultured for one additional day ...
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bioRxiv - Microbiology 2020Quote: ... log phase promastigotes were washed twice with PBS and resuspended at 5.0 x 107 cells/ml in PBS in the presence of 100 µM of D-[U-14C] glucose (250 mCi/mmol, PerkinElmer Inc.). The reaction mixture (200 µl each in triplicates ...
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bioRxiv - Systems Biology 2021Quote: ... cell cultures were seeded and infected at the desired MOI in black 96-well PerkinElmer CellCarrier ultra imaging plates (Perkin Elmer). Infected cells were fixed with 10% neutral buffered formalin (NBF ...
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bioRxiv - Microbiology 2021Quote: ... HEV replication was analyzed by determining the number of GFP-positive cells using fully automated confocal microscopy (Operetta CLS; PerkinElmer Devices).
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bioRxiv - Microbiology 2020Quote: ... CoV-infected cells and control mock-infected cells were incubated for different periods of time with tritiated uridine ([5-3H]uridine, 1 mCi/ml, Perkin Elmer), which was mixed in a 1:1 ratio with double-concentrated medium ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Cell Biology 2021Quote: Parental and SETD2-deleted U2OS cells were seeded into 384-well plates (750 cells in 75 μl per well) using the Janus Liquid Handling Workstation (Perkin Elmer) and incubated at 37°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... and dead cells (stained by PI+) were imaged every hour for the indicated time with the Operetta CLS High-Content microscope (Perkin Elmer) at 40x Air/0.6 NA ...
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bioRxiv - Cell Biology 2021Quote: ... samples of doublets were allocated by semi-automated 3D intensity quantification in the Wnt3a-bead proximal and -distal cell using Volocity (Perkin Elmer) or Fiji (ImageJ) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were imaged and percent of virus infected cells calculated using the Operetta High Content Imaging System and Harmony® High Content Imaging and Analysis Software (PerkinElmer).
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Cancer Biology 2022Quote: MDA-MB-231 cells expressing mTurquoise2-SLBP(18-126) and H1-Maroon1 were seeded on clear flat-bottom 96-well plates (Perkin Elmer), treated with DMSO or PXS5382 40μM ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
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bioRxiv - Neuroscience 2021Quote: ... Absorbance of 100 µl aliquots of solubilized cells was measured at 595 nm with Wallac Victor 1420 microplate reader (Perkin Elmer).
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bioRxiv - Neuroscience 2021Quote: ... the fluorescently labeled axon and dendrites were traced from the PV basket cell soma with a Nipkow spinning disk confocal microscope (Volocity, Perkin Elmer, equipped with an Orca camera ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plate was sealed with a transparent film and cells were imaged with the Operetta High Content Imager (PerkinElmer Life Sciences) using a 20x objective ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... tumors (ca 120,000 cells) consisting of H460 human NSCLC transfected with luciferase and the tdTomato gene (H460 Luc+ tdTomato+, Perkin Elmer France) were injected ectopically into the left flank of 90 mice ...
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bioRxiv - Molecular Biology 2019Quote: HUVEC cells were seeded in 6 cm2 plates (4 x 105 cells/well) and 8 hours after plating were pulsed for 2 hours with tritiated uridine (Uridine [5,6-3H]; Perkin Elmer; NET367001MC) at final concentration of 2 µCi/ml ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with an anti-ERα antibody (F-10) overnight followed by Cy3 Tyramide signal amplification (PerkinElmer-TSA-Plus-Cy3) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed with KREBS solution and fluorescence was read with the plate reader VICTOR® Multilabel Plate Reader (PerkinElmer). Data were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... For neutralization assays 104 293T-hACE2 cells were plated in 100uL media per well in 96 well white-wall white-bottom plates (Perkin Elmer) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
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bioRxiv - Biophysics 2020Quote: ... The undifferentiated cells were seeded to 60% confluency to adhere for 24 h in a CellCarrier-96 Ultra Microplates (Perkin Elmer), which was followed by the media replacement with a fresh media containing FCS reduced to 1% and supplemented with 10 μM trans-Retinoic acid (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
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bioRxiv - Cell Biology 2021Quote: The data was collected in a set of 2 experiments for 9 compounds as follows: 384 well plates (PE cell carrier ultra, PerkinElmer, 6057308) containing 12,000 cells per well in RPMI1640 medium (Sigma ...
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bioRxiv - Immunology 2022Quote: ... incubated for 45 minutes at 37 °C and added to pre-seeded 293 flpin-TMPRSS2-ACE2 cells (made by Adrian Creanga, VRC, NIH) in triplicate in 96-well white/black Isoplates (Perkin Elmer). Following 2 hours of incubation ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells were incubated with the AAVs for 24 hours at 37°C and assayed with Britelite plus (Perkin Elmer, cat#6066766) following the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 30,000 cells were seeded in each well of an ultra-low adhesive U-shaped 96-well plate (CellCarrier™ −96, PerkinElmer) with 200 ml of complete Neurobasal medium ...
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bioRxiv - Immunology 2022Quote: ... the medium was removed from all wells and cells were lysed by the addition of 100 µl of a 1:1 dilution of BriteLite assay readout solution (Perkin Elmer) and 1xDPBS (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... half the medium was removed from all wells and cells were lysed by the addition of 50 µl of BriteLite assay readout solution (Perkin Elmer) to make a a 1:1 dilution into each well ...
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bioRxiv - Biochemistry 2023Quote: ... Binding reactions were incubated for 2 hours and then terminated by washing the solutions over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by wash steps using ice-cold buffer [50 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded at 200 cells per well in 40 μL in the inner-312 wells of 384-well white-walled culture plates (Perkin Elmer) and allowed to attach for 24-hours prior to drug treatment ...
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bioRxiv - Cancer Biology 2023Quote: For 2D adherent studies cells were seeded at 500-1,000 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 48 hours prior to drug treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at 500 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 24 hours prior to drug treatment ...