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Citations for PerkinElmer :
851 - 900 of 1063 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 mL fractions containing soluble inositol phosphates were mixed with 3 mL scintillation cocktail (Ultima-Flo AP) and counted for 5 min in a liquid scintillation counter (Tri-Carb 2910 TR, Perkin Elmer). The lipid inositol extract was counted in a liquid scintillation counter ...
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bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
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bioRxiv - Microbiology 2022Quote: ... in the ratio of 5:95 and analyzed at mid-IR region (400–4000 cm−1) by FTIR using Spectrum GX (PerkinElmer, USA).
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bioRxiv - Microbiology 2022Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [α32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... TZM-bl target cells were seeded at a density of 5 × 103 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times for 5 min in TBS-T buffer and prior to chemiluminescence detection using Western Lightning chemiluminescent substrate (Perkin Elmer). Chemiluminescence signal was detected with an ECL-Imager (INTAS ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... and developed for 10 minutes with fluorescein- or Cy3-conjugated Tyramide amplication system (Perkin Elmer, Shelton, CT). Amplified staining for Aggrecan was performed without detergent (1X PBS only ...
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bioRxiv - Biochemistry 2022Quote: ... ATP concentration was varied from 0 to 1000 mM in presence of 10 nM [3H]ATP (PerkinElmer). The reaction was initiated by adding ATP to the reaction solution containing 0.005 mg/ml Cya ...
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bioRxiv - Neuroscience 2021Quote: ... and the conjugate was detected using the Fluorescein TSA Plus System (1:10, PerkinElmer, Waltham, MA, USA) for 2 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... and 2.5 μCi of [γ32P]-ATP (stock of 6000 Ci/mmol 10 mCi/mL, from Perkin Elmer). Autophosphorylation reactions were performed at 30 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Physiology 2022Quote: ... and 0.01% Triton X-100) in the presence of 10 μCi [32P]-γ-ATP (Perkin Elmer Company) with a final volume of 20 μl ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... Images were acquired within 10 minutes following intraperitoneal injection of 150mg/kg of D-luciferin (PerkinElmer #122799)
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc, Perkin Elmer) in 15μL reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... by intraperitoneal injection and then imaged 10 min later using an IVIS™ Spectrum imaging system (PerkinElmer). Body weight and tumor volume were measured 1-2x weekly.
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bioRxiv - Immunology 2023Quote: ... 10 µl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration ...
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bioRxiv - Neuroscience 2023Quote: ... 7 increasing concentrations (0.15 – 10 nM) of [3H]-SCH23390 (specific activity 83.6Ci/mmol, Perkin Elmer, #NET930025UC, Canada) for D1 binding to generate saturation curves ...
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bioRxiv - Cell Biology 2022Quote: ... were added to cells at a final concentration of 10 μM using a Janus Automated Workstation (PerkinElmer) equipped with a pin tool (V&P Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... for 10 minutes and then imaged using the In Vivo Imaging System (IVIS) Spectrum imaging system (PerkinElmer) and Living Image software (PerkinElmer).
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bioRxiv - Biochemistry 2024Quote: ... Mixture and gel slices were transferred to scintillation vials and 10 mL Hionic-Fluor scintillation fluid (PerkinElmer) was added ...
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bioRxiv - Cell Biology 2024Quote: ... were applied at 10 µg/mL and immediately imaged using Opera Phenix High Content Imaging System (PerkinElmer) at 5× magnification ...
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bioRxiv - Cell Biology 2024Quote: ... Cryosections of 10 μm were cut and the Spectrum Spotlight 400 FT-IR Imaging system (Perkin Elmer) was used to collect IR spectral imaging data in the mid-IR region from 4,000–750/cm at 8/cm spectral resolution and 25 μm spatial resolution ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded overnight with MEM +HIHS cell culture media supplemented with 4 μCi/mL D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET501001MC). Coverslips were washed in isosmotic Basal medium containing (in mM ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Microbiology 2020Quote: Purified Caulobacter ParB-His6 or TetR-His6 (final concentration: 25 μM) were incubated with 5 nM radiolabeled P32-α-CTP (Perkin Elmer), 30 μM of unlabeled cold CTP (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The receptor autoradiography procedure was performed using a linear V1a-R antagonist [125I]-d(CH2)5(Tyr[Me])-AVP (Perkin Elmer, USA) or a linear OXTR antagonist [125I]-d(CH2)5[Tyr(Me)2-Tyr-Nh2]9-OVT (Perkin Elmer ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Molecular Biology 2020Quote: 100 pmol of WT or mutant RNA oligonucleotides (synthesized by Integrated DNA Technologies, Inc.) were 5’- end labeled with [γ32P] ATP (Perkin Elmer, NEG035C001MC) and T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Tumors were isolated from mice and sectioned into 5 micrometer sections for staining with the desired markers (below) using Tyramide Signal Amplification (PerkinElmer, Waltham MA) per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The colonies were grown in 160 μL of TAP medium for 5 days in black clear bottom 96 well plates (CellCarrier-96 Black, 6005550, PerkinElmer Inc, USA), sealed with a paper tape ...
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Developmental Biology 2024Quote: ... Plates (with lid on) were placed in the Opera Phenix High-Content Screening System (Perkin Elmer; prewarmed to 37C and 5% CO2). Images were collected every 30 min for 24 hours.
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bioRxiv - Biophysics 2023Quote: ... stand and a 40 nt oligo-dT at the 5′ end was labeled at the 3′ terminus with [α-32P] dCTP (Perkin Elmer) and annealed to the single-stranded DNA M13mp18(24) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Determination of Gαi and Gαs selective signaling at the 5-HT1AR was performed applying an IP accumulation assay (IP-One HTRF®, PerkinElmer, Rodgau, Germany) according to the manufacturer’s protocol and in analogy to previously described protocols (15 ...
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bioRxiv - Immunology 2023Quote: ... Hybridization signals were detected by fluorescent signal using peroxidase-based Tyramide Signal Amplification-Plus Fluorescein or TSA Plus Cyanine 5 (Perkinelmer, Shelton, CT). Finally ...
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bioRxiv - Systems Biology 2024Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Immunology 2024Quote: ... Monocytes from five healthy donors were resuspended in an RPMI medium at 5 x 105 c/ml and distributed into the wells (100 μL) of white opaque 96-well microplates (Perkin Elmer, PKE_6005299). Macrophage cell cultures were generated by adherence of monocytes to cell culture plasticware as previously described (16) ...
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bioRxiv - Biochemistry 2024Quote: ... Hairpin substrates with a two-nucleotide 5′-overhang end for EM-SAs were assembled by self-annealing oligonucleotides that were 5′-end labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... solution (5 µg/l) was selected as the internal standard and added online during the ICP-MS analysis (NexION 2000, Perkin Elmer, USA). The method detection limits were also checked periodically ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 images of each section were randomly collected and analyzed by the Columbus v.2.4.2 software (Perkin Elmer) with an algorithm adapted for Oil Red-O stained sections ...
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bioRxiv - Cell Biology 2021Quote: ... 3 10-minute TBST washes were performed followed by Western Lightning plus ECL Enhanced Chemiluminescence (Perkin Elmer, MA). Proteins were detected on X-ray film (Fuji ...