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Citations for PerkinElmer :
551 - 600 of 1063 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Neuroscience 2024Quote: Free-floating sections were rinsed 3 times for 2 min each in 0.01 M PBS with 0.2% Triton X-100 and then were blocked for 20 min in TSA Blocking Reagent (Perkin Elmer). Sections were incubated with primary antibodies (Supplementary Table 2 ...
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bioRxiv - Physiology 2024Quote: ... 4 µL of homogenate was briefly incubated with each drug treatment (eight-fold serial dilution ranging from 8 µM to 62.5 nM) in a 0.1% DMSO/50 ppm tween-20/E2 solution in 96-well plates (Perkin Elmer 6005299). A solution of 30 µL PBS ...
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bioRxiv - Microbiology 2020Quote: ... we used THP-1 cells (4×105 cells/ml) adhered in an optical 96-well plate (PerkinElmer) with 50 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Cell Biology 2020Quote: ... 300 μl of each supernatant were mixed with 4 ml of Ultima Gold liquid scintillation cocktail (PerkinElmer) and run on a Hidex SL300 counter ...
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bioRxiv - Plant Biology 2023Quote: ... The same seeds were transferred into weighing cup and weighed with an AD-4 Autobalance (PerkinElmer, Inc.). Single seed weight was subsequently determined by dividing the total weight by the number of seeds.
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bioRxiv - Molecular Biology 2021Quote: ... 10 µM [3H]-SAM (50-80 Ci/mmol, Perkin Elmer Health Sciences), and enzymatic complexes were mixed in the Reaction Buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... then 25 μM GTP and 10 μCi [α32P]-GTP (PerkinElmer, Cat#BLU006H250UC) were added to start the reaction ...
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bioRxiv - Biochemistry 2021Quote: ... coli TrmD (10 μM) and [3H]-AdoMet (Perkin Elmer, 4200 DPM/pmol) at a final concentration of 15 μM in a buffer containing 100 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... Bioluminescence images were captured 10 min later using an imaging system (PerkinElmer). The Shandong University Animal Ethics Research Board approved the animal experiment procedures (SDULCLL2019-2-08).
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Developmental Biology 2020Quote: ... containing 10 μL of MCF 10A culture medium (PerkinElmer Janus with Nanohead). MCF 10A cells were then plated in 40 μL of MCF 10A culture medium to achieve a final concentration of 10 μM for each compound and 0.25 % (vol/vol ...
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bioRxiv - Neuroscience 2020Quote: ... section were incubated with TSA Cy3 (1:300, 10 min, Perkin Elmer). Finally ...
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bioRxiv - Physiology 2022Quote: ... then incubated for 10 min with radioactive 2-DG (PerkinElmer NEC 720A250UC). Cells were washed in cold Krebs-Ringer phosphate HEPES (KRPH) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 min with horseradish peroxidase-reactive OPAL fluorescent reagents (Perkin Elmer). Slides were washed between staining steps with Bond Wash (Leica ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was measured using an assay kit (Lot: 122799-10, PerkinElmer). Images of tobacco leaves were recorded by a CCD system (Feng et al. ...
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bioRxiv - Systems Biology 2020Quote: ... Half of each sample (5×103 cells) was transferred to a luminescence plate (PerkinElmer Optiplate-96). Cells from the remaining half were pelleted and equal volume (to match the sample ...
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bioRxiv - Developmental Biology 2021Quote: ... Indexed libraries were pooled and size-selected to 320 bp ± 5% with a LabChip XT (PerkinElmer). Libraries were loaded onto a single-read flow cell and amplified on a cBot (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... and tyramide signal amplification (TSA) of the TSA Plus Cyanine 5 (Cy5) detection kit (NEL745001KT, PerkinElmer) were used for a single color FISH experiment.
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to different concentrations of purified YegI (0.0625,0.125,0.25,0.5,1,2 μM ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to 1 μM of purified YegI in 10 μl of reaction buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1/20th of the annealed substrates were 5′-end-labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... Northern probes were 5’ 32P-end labeled with 0.3 mCi of y-32P ATP (Perkin Elmer) with 10 U of T4 Polynucleotide kinase (NEB ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 µL of Alamar Blue was dispensed with a FlexDrop precision dispenser (Perkin Elmer, Boston, MA). After an additional incubation of 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Carried out fluorescence sensitization by incubating the samples in 1% Cyanine 5 regent (Perkin Elmer FP1171) with amplification diluent (Perkin Elmer FP1135 ...
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bioRxiv - Cancer Biology 2024Quote: ... Carried out fluorescence sensitization by incubating the samples in 1% Cyanine 5 regent (Perkin Elmer FP1171) with amplification diluent (Perkin Elmer FP1135 ...
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bioRxiv - Microbiology 2020Quote: ... Further 160μl of DNS solution was added in each well and incubated at 100°C for 20 min followed by reading at 570nm in multimode plate reader (Perkin Elmer). All the quantitative assays were done in triplicates and the activity was estimated using the standard graph of glucose.
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bioRxiv - Developmental Biology 2022Quote: ... For signal detection sections were incubated with the channel specific HRP for 20 min at 40 °C and incubated with the respective fluorophores (Perkin Elmer: Fluorescein ...
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bioRxiv - Biochemistry 2022Quote: ... was licensed at a final concentration of 7.5 ng/µl for 20 min at room temperature in HSS supplemented with trace amounts of [α-32P]dATP (Perkin Elmer). DNA replication was initiated by addition of two volumes of NPE mixture to one volume of licensing mixture ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was started by incubating with 0.1 mM ATP containing 20 μCi of [γ-32P] ATP (3000 Ci/mmol; Perkin Elmer) containing 1 mM of cold ATP at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Michaelis-Menten kinetics of the untagged purified MtCM variants were determined by a continuous spectroscopic chorismate depletion assay (Lambda 20 UV/VIS spectrophotometer, Perkin Elmer). The enzymes were diluted to working stock solutions in 20 mM potassium phosphate ...
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bioRxiv - Synthetic Biology 2023Quote: ... female NMRI mice with a bodyweight of around 20 g were administered intraperitoneally with 150 mg/kg D-luciferin (PerkinElmer, 122799). After 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... images were taken with an Operetta® CLS High-Content Imaging System with 20-fold magnification and analyzed using the Harmony® High-Content and Imaging Analysis Software (PerkinElmer). 26 image fields per well were acquired.
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bioRxiv - Molecular Biology 2024Quote: ... tRNA aminoacylation was performed in buffer A supplemented with 1 mM ATP in the presence of 20 µM [3H]-phenylalanine (specific activity 136 dpm/picomole, Perkin Elmer) and either purified E ...
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bioRxiv - Biochemistry 2024Quote: We load equal amounts of 20 µl sample volume for the total protein concentration titration in 384 well plates (PhenoPlate, PerkinElmer, USA). The well plates were chosen due to the superior non adhesive properties that allow to assume spherical shape of the condensates ...
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bioRxiv - Cell Biology 2020Quote: ... and all qualitative imaging of Kif19 plasmids were conducted on a 4-D spinning-disk confocal microscope (PerkinElmer) with 60x (1.4 NA ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET measurements were routinely performed following overnight incubation at 4°C using an EnVision Reader (Perkin Elmer). Values were collected as the background subtracted ratio between fluorescence emission at 665 nm and 615 nm where the background signal corresponds to the ratio (665/615 ...
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bioRxiv - Neuroscience 2021Quote: ... the free-floating sections were incubated overnight at 4°C with Streptavidin-Texas Red (1:400, NEL721001EA PerkinElmer). Finally ...
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bioRxiv - Microbiology 2021Quote: KN99α was grown as described above in MM supplemented with 2 μCi/ml 3H-NE and 1.0 mM unlabeled NE or a mixture of 1.0 mM unlabeled NE and DA or with 4 μCi/mL (0.0001 mM) Dihydroxyphenylethylamine 3,4-[Ring-2,5,6-3H] (3H-DA, Perkin Elmer NET673250UC) and 1.0 mM unlabeled DA or 1.0 mM unlabeled NE and DA for 10 d at 30°C ...
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bioRxiv - Immunology 2022Quote: ... Prior to the immunization all mice were anesthetized by isoflurane inhalation with RAS-4 Rodent Anesthesia System (PerkinElmer). Mice were euthanized on day 28 for terminal blood collection and spleens were harvested for cellular assays ...
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bioRxiv - Plant Biology 2022Quote: ... dissolved in 0.1% nitric acid and 4 mL Optima Gold XR scintillant (Perkin Elmer, Glen Waverley, VIC, Australia) was added prior to counting for two minutes using a Liquid Scintillation Counter (LS6500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were fixed in 4% PFA and imaged with the Opera Phenix High Content Screening System (Perkin Elmer), acquiring at least 10 images per well ...
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bioRxiv - Cell Biology 2023Quote: ... Live imaging was done 4-8h later using a spinning disk confocal microscope (Perkin Elmer Ultra View ERS) equipped with a heating chamber (37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... or 4 µl of suspension was carefully distributed in the centre of an optical-bottom 96-well (PerkinElmer) for immunofluorescence experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... filters were placed into scintillation vials with 4 mL Emulsifier-safe liquid scintillation fluid (Perkin Elmer, Waltham, MA) and analyzed using a liquid scintillation counter ...
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bioRxiv - Cell Biology 2023Quote: ... the rabbit reticulocyte lysate master mix was supplemented with EasyTag L-35-Methionine (4% final reaction volume; PerkinElmer). Canine rough pancreatic microsomes (cRMs ...
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bioRxiv - Bioengineering 2022Quote: ... Protein A-conjugated acceptor beads (#6760617C, PerkinElmer©; 10 μL, 50 μg/mL) were then added to the vials ...
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bioRxiv - Bioengineering 2022Quote: ... Ni-NTA coated donor beads (6760619C, PerkinElmer©; 10 μL, 50 μg/mL) were added and the plate was further incubated at 23 °C for 60 min in a dark box ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were started by adding 10 μCi of [γ-32P]ATP (PerkinElmer, BLU002A500UC), and 10 μl samples were removed at different times ...
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bioRxiv - Microbiology 2020Quote: ... 50 µM GTP and 10 µCi of α-32P labelled GTP (Perkin Elmer).