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• Surface-associated antigen induces B-cell permeabilization and lysosome exocytosis facilitating antigen uptake and presentation to T-cells

  Fernando Y. Maeda, et al., bioRxiv - Cell Biology 2021

  Quote: ... Cells were washed and imaged at 37°C with 5% CO2 in the presence of 50 μg/ml PI and 10 µg/ml FM1-43FX using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective). Images were acquired at 1 frame/30 s for 60 min and analyzed using Volocity (PerkinElmer).