Labshake search
Citations for PerkinElmer :
551 - 600 of 601 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
-
bioRxiv - Biochemistry 2024Quote: ... For competition binding experiments cell membranes expressing h5-HT3A WT or mutant receptors were incubated in 250 µL 10 mM HEPES buffer (pH 7.4) containing 0.3 nM [3H]-GR65630 (Perkin Elmer, Waltham, MA) and varying concentrations of VTX or analogues ...
-
bioRxiv - Cancer Biology 2022Quote: ... Image analysis was performed on the maximum projection image of 10 whole-well z-planes of the confocal imaging using Harmony software (Perkin Elmer).
-
bioRxiv - Biochemistry 2022Quote: ... then centrifuged at 61 g for 10 seconds and the data were acquired in a Victor X 5 plate reader (Perkin Elmer) with an excitation wavelength of 480 nm and an emission wavelength of 535 nm ...
-
bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
-
bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
-
bioRxiv - Plant Biology 2023Quote: ... Stained SDS-PAGE bands were excised and transferred to scintillation vials and emulsified in 10 ml of Ultima Gold XR scintillation liquid (Perkin Elmer) in darkness at room temperature overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... Dissociated cells were resuspended in 7.5 mg/mL BME-2 matrix gel at 2-5×104 cells/mL and seeded in 10 μL droplets to individual wells of 96-well Cell Carrier Ultra plates (Perkin Elmer). Once settled ...
-
bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
-
bioRxiv - Microbiology 2024Quote: ... 10 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) for until OD600 reached ∼1.5-2 ...
-
bioRxiv - Neuroscience 2024Quote: ... Homogenization buffer was prepared as a mix of 10% ‘Lysis & Detection Buffer’ and 90% ‘Stimulation Buffer’ 1× from the IP-One Gq kit (Perkin Elmer). While the exact formula of this buffers is proprietary ...
-
bioRxiv - Cancer Biology 2024Quote: ... the mice were intraperitoneally injected with 150mg/Kg of D-luciferin and 10 min later imaged using IVIS™ Spectrum imaging system (PerkinElmer) while under anesthesia (2% isoflurane inhalation) ...
-
bioRxiv - Biophysics 2024Quote: ... A six-point standard curve from 200 ppb to 1 ppb (200, 100, 50, 10, 5, 1) was prepared from elemental standards (Perkin Elmer), plus a 0-ppb blank ...
-
Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-viral hsa-miR-132 ProcessingbioRxiv - Molecular Biology 2024Quote: ... 1 µl of a 1 µM of miRNA working solution was incubated with 1.5 µl ATP ([γ-32P]-6000 Ci/mmol, 10 mCi/ml, Perkin Elmer), 1 µl RNase inhibitor (Promega ...
-
bioRxiv - Bioengineering 2024Quote: ... Biopsy samples were γ-counted using an open-window protocol for 10 min using a National Institute of Standards and Technology source– calibrated system (Wizard2; Perkin Elmer). These processed CT volumes and the activity measured from the gamma counter were then used as input for simulations of Radium-223 treatment.
-
bioRxiv - Cancer Biology 2021Quote: ... the mice underwent BLI beginning at 10 days after implantation and every week thereafter with the In Vivo Imaging System (IVIS, PerkinElmer, Waltham, MA), and luminescence flux was recorded to assess tumor growth ...
-
bioRxiv - Cell Biology 2020Quote: ... was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer, USA). Measurements were done at least in triplicates and relative luciferase units (RLU ...
-
bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
-
bioRxiv - Cancer Biology 2020Quote: Resulting cells were ultra-concentrated in 10 μl of DPBS and plated onto wells of a CellCarrier-96 Ultra Microplates (PerkinElmer, Cat# 6055302). Plates were then centrifuged at 1800g for 10 minutes to allow the cells stick to the surface of the wells ...
-
bioRxiv - Microbiology 2022Quote: ... an appropriate concentration of DSF/BDSF/trans-BDSF was mixed with the RpfC liposomes and the resulting solution was incubated with 100 µM ATP containing 10 µCi [γ-32P]ATP (PerkinElmer, USA) in autophosphorylation buffer (50 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2021Quote: ... Plates sat at room temperature for an additional 10 min in the dark before samples were read on an EnVision XCite plate reader (PerkinElmer # 2105-0020).
-
bioRxiv - Biochemistry 2021Quote: ... metabolic labeling was started by replacing the medium with phosphate-free DMEM containing 10% dialyzed FBS and 1mCi/mL 32P orthophosphate (PerkinElmer, Waltham, MA). After labeling for 8 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... Plates were shaking at 999 rpm and 28°C and growth was measured by monitoring OD600 every 2 h for 10 h using a plate reader (2300 EnSpire™, Perkin Elmer).
-
bioRxiv - Physiology 2023Quote: ... was prepared by mixing 40 µL homogenate with an equal volume Nanoluc reaction buffer (PBS: NanoGlo Buffer: NanoGlo substrate furimazine, 30: 10: 0.2) in a black perkin elmer plate (6065400, PerkinElmer, Waltham, MA, USA). Within 2 min of buffer addition ...
-
bioRxiv - Microbiology 2023Quote: ... The 3280 compounds were tested in a final concentration of 10 µM with the rhlA-gfp [30] reporter strain in 96 wells microtiter dish (Black Isoplate®, Perkin Elmer). The cells were added in a final concentration of OD600=0.1 and final volume of 200 µl in ABGC medium and incubated at 37°C with shaken (200 rpm) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The membranes were washed in TBS-T for 3x 10 minutes and developed with Western Lightning Plus-ECL reagent (Perkin Elmer NEL104). To produce in vitro the Sp5 protein ...
-
bioRxiv - Biochemistry 2024Quote: ... Fluorescence spectra between 470 to 600 nm at an excitation wavelength of 450 nm (Perkin Elmer LS 55, PM 750, slits 10), were immediately acquired after the dilution and homogenization steps ...
-
bioRxiv - Microbiology 2024Quote: ... RNA transcripts from the ffh and zapA promoters were labeled with 2– 10 µCi of [α-32P] ATP or [α-32P] UTP (PerkinElmer), respectively ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the plates incubated for 10 min prior to luminescent detection in a Victor 2 microplate reader (Perkin Elmer, Waltham, MA, USA) and the signal was quantified at 595 nm ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by 15 µL of 6 M sodium hydroxide for 10 min at room temperature before reading plates at 420 nm on an EnVision plate reader (PerkinElmer; Waltham, MA). Initial velocities for each inhibitory BCKAs concentration were derived by regression fitting of timepoints within distinct linear ranges of each time course ...
-
bioRxiv - Bioengineering 2021Quote: ... The volume of samples was adjusted to 10 mL by adding ultra-pure water and Gd concentration in each sample was measured using ICP-Mass Spectrometer (PerkinElmer, NexION® 2000). A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 80-120 mg/kg body weight) / xylazine (Xy, 5-10 mg/kg body weight) cocktail) using an IVIS Spectrum In Vivo Imaging System (Perkin Elmer, Rodgau, Germany) with images acquired using the Living Image® software package (Perkin Elmer).
-
bioRxiv - Plant Biology 2021Quote: ... Fluorescence spectra of solutions diluted 10-fold were recorded with 3.5 mL Suprasil® quartz optical cells of 10 mm pathlength using a LS-50B spectrofluorimeter monitored with UVWinlab 5.1 software (Perkin Elmer, Waltham USA). For each compound ...
-
bioRxiv - Plant Biology 2020Quote: ... detection limit 5 ng.L−1) and K+ content by ICP-AES (-Atomic Emission Spectrometry, OPTIMA 8300, Perkin Elmer, quantification limit 10 μg.L−1).
-
bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
-
bioRxiv - Genomics 2020Quote: Monocytes from three unrelated healthy participants were plated in 30 µL of RPMI 1640 and 10% FBS on CellCarrier-384 well Ultra Microplates coated with PDL (PerkinElmer; cat. no. 6057500) at a concentration of 100,000 cells/well ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μCi of radioactive Pi solution (10 μL from a 2 mCi K2H32PO4 at a concentration of 2 mM of K2H32PO4, PerkinElmer cat. no. NEX055) was added to 1 mL of cell suspension ...
-
bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
-
bioRxiv - Cancer Biology 2023Quote: ... was injected intraperitoneally at a dose of 150 μg/g and incubated for 10 min before scanning in the IVIS® Spectrum In Vivo Imaging System (PerkinElmer, Waltham, MS).
-
bioRxiv - Molecular Biology 2023Quote: ... The PCR tube was incubated at 80 °C for 10 minutes before cooling to 12 °C using a GeneAmp 2400 thermal cycler (Perkin Elmer, Waltham MA).
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... 5-HT uptake was initiated by the addition of 10 nM [3H] 5-HT (Cat No: NET498001MC; 24.82 Ci/mmol: Perkin Elmer, Santa Clara, CA). and the uptake was terminated at 5 min with the addition of fluoxetine followed by rapid filtration over GF-B filters ...
-
bioRxiv - Biochemistry 2024Quote: ... The lysed cells were diluted 1:2 with assay buffer after which 10 μL/well was pipetted to a white 384-well OptiPlate (PerkinElmer, Waltham, MA, USA). Next ...
-
bioRxiv - Bioengineering 2024Quote: ... MDA-MB-231 cells expressing HMGB1-GFP and CRT-RFP (stained MDA cells) were cultured in 100 µl of RPMI medium (10% FBS and 1% penicillin) in 96-well plate (PerkinElmer ViewPlate – 96 TC) at the density of 5 × 103 cells/well ...
-
bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
-
bioRxiv - Cancer Biology 2024Quote: ... The tumor burden was quantified weekly by bioluminescent signal intensity on isoflurane-anesthetized mice that received a subcutaneous injection of D-luciferin (150 μg luciferin/1 g mouse body weight) 10 min prior to IVIS® imaging (PerkinElmer, Waltham, MA,USA). Survival data were presented and reported in Kaplan–Meier plots.39
-
bioRxiv - Biophysics 2020Quote: ... and SYBR Gold to the specified dye concentrations (0.03 to 60 μM) and analyzed the sample in a 10 × 2 mm2 cuvette (Perkin Elmer UV/VIS Spectroscopy Cell B0631122) for 5 min for each condition ...