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Citations for PerkinElmer :
501 - 550 of 601 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and transfected cells were plated evenly across 10 wells of poly-D-lysine-coated 96-well PhenoPlates (PerkinElmer; Cat# 6055508). Half-media changes of BMDM medium containing 50ng/mL M-CSF were performed every other day and endpoints were assessed until 14 days after transfection.
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bioRxiv - Biochemistry 2024Quote: ... then incubated with Ham’s F-10 media supplemented with 1.5 mCi/well myo[2-3H(N)] inositol (Perkin Elmer/Revvity) for 16-18 h ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min before being imaged with an Ultra View VOX confocal laser scanning microscope (Perkin Elmer, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... across 25 x 25 fields of view with a 10% overlap were acquired per well on an Opera Phenix High Content Screening System (PerkinElmer) using a 40x or 63x/1.15 NA water-immersion objective ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg/mL of SYNs were added to hMG plated on a CellCarrier™-96 Ultra Microplate (Perkin Elmer®) and imaged using 20x objective of a Zeiss SP05 confocal microscopy ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... add 10 mL scintillation solution and measured the radioactivity retained on the membranes in liquid scintillation counter Tri-carb 4910TR (Perkin Elmer) [44].
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bioRxiv - Developmental Biology 2021Quote: ... 10-20 gastruloids per condition were then transferred into a well of a flat-bottom CellCarrier-96 ultra plate (PerkinElmer, 6007008) and imaged on an Opera Phenix HCS system (PerkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: ... the baseline BRET signals were read immediately at 470 nm and 535 nm for 10 cycles using an EnVision® multimode plate reader (PerkinElmer). Following the agonist addition ...
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bioRxiv - Biochemistry 2021Quote: ... All filters were dissolved in 10 ml Emulsifier Safe scintillation fluid and counted using a Tri-Carb A4810TR Liquid Scintillation Analyzer (Perkin Elmer). Experiments were performed in triplicate with fresh cultures.
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bioRxiv - Biochemistry 2021Quote: ... Each filter was placed in 10 ml Emulsifier Safe scintillation fluid and counted using a Tri-CarbA4810TR Liquid Scintillation Analyzer (Perkin Elmer). CodB concentration was quantified based on the GFP fluorescence ...
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bioRxiv - Cell Biology 2021Quote: ... Images (at least 10 fields per well) of hiPSC-CMs were acquired using Operetta® High-Content Imaging System (Perkin Elmer) with a 20x long working distance (WD ...
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bioRxiv - Biochemistry 2021Quote: 10 pmol of single-stranded DNA (Eurofins MWG Operon, Germany) were labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) by incubation with terminal deoxynucleotidyl transferase (TdT ...
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bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then treated for 10 minutes with indicated compounds before addition of 20 μCi of 32P-ortho-phosphoric acid (Perkin Elmer). Cells were incubated for 1 hour with media containing drugs and 32P-ortho-phosphoric acid ...
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bioRxiv - Bioengineering 2021Quote: ... Mice were administered 3.3 mg D-Luciferin in the morning of day 3 and luminescence was recorded after 10 minutes by an In Vivo Imaging System (IVIS, Perkin Elmer) followed by i.v ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 100 μL were transferred daily (up to 10 days post dormancy induction) to a 96 well plate where 5 μCi 3H-hypoxanthine (Perkin Elmer), 5 μCi 3H-adenosine (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... Solutions were then cleared by centrifugation at 4000 g for 10 min and the supernatant analyzed in a black 96 well microtiter plate using an EnSpire (PerkinElmer, USA) microplate reader with an Excitation/Emission wavelength of 630 nm/ 660 nm (with 9 nm band-passes around the maximum) ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Microbiology 2021Quote: ... The plate was shaken at 300 RPM at room temperature for 10 minutes after which RLUs were read on an EnSight (Perkin Elmer) microplate reader ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 minutes at room temperature and the luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Immunology 2021Quote: Vero-E6 were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20’000 cells per well ...
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bioRxiv - Neuroscience 2021Quote: ... was added to the chamber for 30 min to inhibit transcription after which slices were transferred to fresh aCSF containing ~10 mCi/ml [35S] Met/Cys (Perkin Elmer) for an additional 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CCK hybridization was revealed by a TSA reaction using fluorescein isothiocyanate (FITC)-labeled tyramide (1:100 for 10 minutes, NEL741001KT Perkin Elmer). After several washes ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were pulsed (0.45MBq/106 cells) for 10 min with 35S Cys/Met (EasyTagTM Express Protein Labelling, Perkin Elmer, Beaconsfield, UK) in DMEM without Cys/Met ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
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bioRxiv - Plant Biology 2020Quote: ... was incubated with oligonucleotide probes (0.01 pmol-10 fmol) 5’-radiolabeled with [y-32P]ATP (5000 Ci/mmol; PerkinElmer Life Science). Different dsDNA structures were prepared by annealing the radiolabeled sense oligonucleotides with a twofold excess of unlabeled complementary oligonucleotide and purified on non-denaturing polyacrylamide gels ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
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bioRxiv - Immunology 2021Quote: Vero-E6 and Vero E6-TMPRSS2 were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 2e4 cells per well ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: Vero E6 expressing TMPRSS2 or not were grown in DMEM supplemented with 10% FBS and seeded into white 96 well plates (PerkinElmer, 6005688) at a density of 20 thousand cells per well ...
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bioRxiv - Immunology 2021Quote: ... The plates were shaken at 300 rpm at room temperature for 10 minutes and relative light units (RLUs) were then read on an EnSight microplate reader (Perkin Elmer). Percent neutralization was determined by subtracting the mean background (uninfected cells with luciferase substrate alone ...
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bioRxiv - Immunology 2021Quote: ... incubated for 10 minutes at room temperature and the luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: Glassware and plasticware used for RNA gel electrophoresis were soaked in 10% bleach for 5 minutes before washed by COUNT-OFF™ Surface Cleaner (PerkinElmer) and liberally rinsing in double-distilled water ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... We verified that these cells did not lose viability after overnight culture in serum-free medium relative to medium with 10% serum as determined by cell-based measurements of CBG bioluminescence in an IVIS Lumina (Perkin Elmer). We injected 1 × 105 breast cancer cells per mouse (n = 5 per each cell type) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were carefully added and plates were incubated for 10 min before luminescence reading in the EnVision 2103 Multilabel Reader (Perkin Elmer).
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bioRxiv - Immunology 2023Quote: ... Iodine-125 (125I) labeling was performed by incubating DTPA-bsAbs in iodogen coated Eppendorf tubes with Na125I (10−5 M NaOH, Perkin Elmer) in 0.5 M phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were incubated for 10 min on a shaker in the dark before measuring luminescence on the EnVision 2102 Multilabel reader (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 minutes before changing to MOPS-buffered ‘Air medium’ with 10% serum but lacking luciferin and moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Cancer Biology 2024Quote: ... From each well was transferred 10 µL of the lysates to a 384-well Optiplate™ (Perkin Elmer, catalog no. 6007290). To each well was added Acceptor Mix (5 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng was used for random priming reactions in the presence of the radiolabeled nucleotide [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml, Perkin Elmer) using a random primer DNA labeling kit (TaKaRa ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were briefly vortexed and added to 20 mL liquid scintillation vials to which 10 mL of Ultima Gold was added (Perkin Elmer). The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer) ...