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Citations for PerkinElmer :
551 - 587 of 587 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
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bioRxiv - Cell Biology 2020Quote: ... Five μl of this sample was then added to a microscope slide and imaged using an Ultraview Vox spinning-disc confocal microscope (PerkinElmer, Waltham, MA) with a 60× objective lens.
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bioRxiv - Plant Biology 2020Quote: ... the caps were screwed on and they were loaded into the microwave (Titan MPS Microwave Sample Preparation System, PerkinElmer, Waltham, MA, USA). Samples were processed in random batches of 15 plus a blank (capacity of the Titan MPS system) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Radioactively labelled protein samples were then precipitated using trichloroacetic acid (TCA) and their radioactive counts measured by liquid scintillation using a MicroBeta2 (PerkinElmer, Waltham, MA) to quantify soluble and total luciferase yields as previously reported (Jewett et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates sat at room temperature for an additional 10 min in the dark before samples were read on an EnVision XCite plate reader (PerkinElmer # 2105-0020).
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bioRxiv - Plant Biology 2022Quote: ... and the eluted DNA quality was assessed with Caliper LabChip GX for DNA samples using the High Sensitivity Assay (PerkinElmer, Inc. USA) and quantified using a Quant-iT dsDNA High Sensitivity Assay Kit on a Qubit fluorometer (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... decays per minute (DPMs) of radioactivity in each sample was read by a Tri Carb 4910 TR Liquid Scintillation Counter (PerkinElmer, Waltham, MA). Specific binding of [35S]GTPγS to G-proteins was calculated for each sample by subtracting the number of DPMs quantified in non-specific binding samples (i.e ...
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An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08) as per the manufacturer’s protocol and sequenced on the Nextseq 500 instrument (Illumina ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were counterstained with DAPI nuclear counterstain and imaged using the Vectra Polaris multispectral imaging system (Perkin Elmer, Life Sciences, Zaventem, Belgium) and analysed using QuPath ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the concentration of each sample was determined using Lunatic plates (Unchained Labs, Pleasanton, CA) read on a DropletQuant spectrophotometer (PerkinElmer, Waltham, MA).
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bioRxiv - Molecular Biology 2023Quote: ... Root and shoot samples were collected separately and Al content qualified by inductively coupled plasma mass spectrometry (ICP-MS, PerkinElmer, NexlON 2000).
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bioRxiv - Physiology 2024Quote: ... All metal concentrations in each water sample were measured using the Perkin Elmer Optima 8000 Inductively Coupled Plasma Optical Emission Spectrometer (PerkinElmer, Connecticut, USA). The standards used ranged from 7 to 1000 µg Nd/L (Inorganic Ventures ...
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bioRxiv - Neuroscience 2020Quote: ... Reaction termination and detection were performed using 1X LANCE detection buffer with 20 mM EDTA and 2 nM LANCE Ultra Europium anti-phospho Glycogen Synthase (Ser641) (Perkin Elmer #TRF0220-M).
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bioRxiv - Biochemistry 2023Quote: ... Ten μl of these dilutions or 10 μl of G protein storage buffer without nucleotides were pipetted into the corner of a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290). Ten 10 μl of 0.1 mM Coelenterezine 400a diluted in BRET buffer (140 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... The lysed cells were diluted 1:2 with assay buffer after which 10 μL/well was pipetted to a white 384-well OptiPlate (PerkinElmer, Waltham, MA, USA). Next ...
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bioRxiv - Bioengineering 2021Quote: ... The volume of samples was adjusted to 10 mL by adding ultra-pure water and Gd concentration in each sample was measured using ICP-Mass Spectrometer (PerkinElmer, NexION® 2000). A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min ...
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bioRxiv - Bioengineering 2021Quote: ... the absorbance of the samples and controls was measured at a wavelength of 570 nm using a plate reader (Multilabel Reader, Perkin Elmer, Waltham, USA). The average of the absorbance values of the 0 % viability control was subtracted from all other values which were then normalized to the growth control (100 % viability control) ...
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bioRxiv - Molecular Biology 2022Quote: ... was added for 20 min, and glucose transport was initiated by addition of [3H]2DG (0.25 μCi, 50 μM) and [14C]mannitol (Perkin Elmer, 0.036 μCi/sample) for the final 5 min of the assay to measure steady-state rates of 2DG uptake ...
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Zoology 2021Quote: ... was then quantitatively evaluated simultaneously in all samples of both species for any given incubation time using the ATPlite assay (PerkinElmer, catalog no. 6016943) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Quality and quantity of the extracted RNA samples were analyzed with LabChip GX Touch HT RNA Assay Reagent Kit (PerkinElmer, Waltham, MA, USA) and Qubit RNA BR kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... samples were diluted 1:10 in reagent-grade H2O and iron concentrations were quantified from the diluted samples using a Perkin Elmer Avio 200 mass spectrometer (Perkin Elmer, Waltham, MA) and compared to a standard curve ...
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bioRxiv - Biochemistry 2023Quote: ... Each of the tissue samples were transferred to gamma counter tubes and counted on a Wallac Wizard 1480 Gamma Counter (Perkin Elmer, Waltham, MA). Count data were converted to percent injected dose per gram using a dose calibration curve.
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bioRxiv - Neuroscience 2023Quote: ... the same cell samples were further lysed and digested with 2% HNO3 for intracellular iron content quantification using an ICP-AES instrument (Perkin Elmer Optima 8000).
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bioRxiv - Bioengineering 2023Quote: ... Cardiomyocyte area and cardiomyocyte number were quantified by identifying cell membranes through staining tissue samples with fluorescent-tagged wheat germ agglutinin (WGA) and quantified using Mantra™ quantitative pathology workstation (PerKinElmer, Waltham, MA)17 ...
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bioRxiv - Genetics 2024Quote: ... The samples were stored at -20°C until genomic DNA was extracted using the semi-automated Chemagen extraction robot (PerkinElmer Chemagen Technologie GmbH). DNA concentration was determined by either DeNovix DS-11 Spectrophotometer (DeNovix Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... with or without EcoRI hairpin oligo or NAD+ in histone methyltransferase buffer and 6 µM of radiolabeled [3H] AdoMet (Perkin Elmer Life Science # NET155V001MC). Recombinant human histone H4 (New England Biolabs # M2504S ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Microbiology 2020Quote: ... signals detected in four spots per well from three independent samples was quantified using the Operetta high content screening system (Perkin Elmer, Waltham, MA, USA) and the built-in Harmony software.
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bioRxiv - Neuroscience 2022Quote: ... Cell counts of brain sections and cultured cells were performed in a blinded and randomized manner in control or experimental samples using Volocity (PerkinElmer Demo Version 6.0.1, RRID:SCR_002668). For brain sections ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was then extracted from 2 ug of total RNA per sample using NEXTFLEX® Poly(A) Beads 2.0 (Perkin Elmer, Austin, Texas, USA). Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... in the digested samples were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) using an 5300 Optima DV spectrometer (Perkin Elmer, Shelton, CT, United States), while trace metals and metalloids by inductively coupled plasma mass-spectrometry (ICP-MS ...
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bioRxiv - Biophysics 2020Quote: ... and SYBR Gold to the specified dye concentrations (0.03 to 60 μM) and analyzed the sample in a 10 × 2 mm2 cuvette (Perkin Elmer UV/VIS Spectroscopy Cell B0631122) for 5 min for each condition ...
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Element partitioning, element localisation and transcriptome responses of date palms exposed to NaClbioRxiv - Plant Biology 2024Quote: ... concentrations of digested samples were determined by ICP-MS (ELAN DRCe, PerkinElmer, Waltham, MA, USA. [Experiment 1] or Nexion 1000, PerkinElmer, Waltham, MA, USA [Experiment 2]). Blank digestions were performed to determine background concentrations of elements and a tomato leaf standard (Reference 1573a ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... collected water samples (45 ml in 50 ml Falcon tubes, with small aerobic headspace) were spiked with 5 µCi of NaH14CO3 (Perkin Elmer, specific activity 56 mCi mmol−1). The samples were incubated for 24 h at ∼ 20 °C in the dark ...